Ctcf
entrez
13018
canonical
uniprot
Q61164
canonical
ensembl
ENSMUSG00000005698
resolver
ensp
ENSMUSP00000005841
resolver
ensp
ENSMUSP00000268288
resolver
ensp
ENSMUSP00000268289
resolver
ensp
ENSMUSP00000268290
resolver
ensp
ENSMUSP00000268291
resolver
ensp
ENSMUSP00000268293
resolver
ensp
ENSMUSP00000268298
resolver
ensp
ENSMUSP00000268299
resolver
enst
ENSMUST00000005841
resolver
genesymbol
Ctcf
resolver
uniprot_entry
CTCF_MOUSE
resolver
activity_regulation
ACTIVITY REGULATION: DNA-binding selectivity is regulated by Jpx RNA, which targets low-affinity CTCF-binding sites and displaces CTCF from DNA by competitive inhibition CTCF. {ECO:0000269|PubMed:34856126}.
uniprot
proteins
amino_acid_sequence
MEGEAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDSSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKKGVKKTFQCELCSYTCPRRSNLDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKPFKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSGTMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHKNEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKCGKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSEENAEPDLDDNEEEEEPAVEIEPEPEPQPQPPPPPQPVAPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKEPDAEPAEGEEEEAQAATTDAPNGDLTPEMILSMMDR
uniprot
proteins
function
FUNCTION: Chromatin binding factor that binds to DNA sequence specific sites and regulates the 3D structure of chromatin (PubMed:26276636, PubMed:26527277, PubMed:28388437, PubMed:34856126). Binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures, such as the nuclear lamina (PubMed:26276636, PubMed:26527277, PubMed:28388437, PubMed:34856126). Defines the boundaries between active and heterochromatic DNA via binding to chromatin insulators, thereby preventing interaction between promoter and nearby enhancers and silencers (PubMed:28388437). Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus (PubMed:16614224). On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2 (PubMed:16614224). Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory (PubMed:16614224). Regulates asynchronous replication of IGF2/H19 (PubMed:17329968). Plays a critical role in gene silencing over considerable distances in the genome (PubMed:16951251). Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones (By similarity). Inversely, binding to target sites is prevented by CpG methylation (By similarity). Plays an important role in chromatin remodeling (By similarity). Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping (By similarity). Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription (PubMed:16951251). When bound to chromatin, it provides an anchor point for nucleosomes positioning (By similarity). Seems to be essential for homologous X-chromosome pairing (PubMed:17952071). May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation (PubMed:11743158, PubMed:15669143, PubMed:17952071). May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation (PubMed:11743158, PubMed:15669143, PubMed:17952071). Involved in sister chromatid cohesion (By similarity). Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites (By similarity). Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis (By similarity). Acts as a transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene (By similarity). Also binds to the PLK and PIM1 promoters (By similarity). Acts as a transcriptional activator of APP (By similarity). Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression (By similarity). Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription (PubMed:18614575). Seems to act as tumor suppressor (By similarity). {ECO:0000250|UniProtKB:P49711, ECO:0000269|PubMed:11743158, ECO:0000269|PubMed:15669143, ECO:0000269|PubMed:16614224, ECO:0000269|PubMed:16951251, ECO:0000269|PubMed:17329968, ECO:0000269|PubMed:17952071, ECO:0000269|PubMed:18614575, ECO:0000269|PubMed:26276636, ECO:0000269|PubMed:26527277, ECO:0000269|PubMed:28388437, ECO:0000269|PubMed:34856126}.
uniprot
proteins
mass_dalton
83745
uniprot
proteins
mutagenesis
MUTAGEN 74; /note="K->R: No sumoylation."; /evidence="ECO:0000269|PubMed:19029252"; MUTAGEN 698; /note="K->R: No sumoylation."; /evidence="ECO:0000269|PubMed:19029252
uniprot
proteins
post_translational_modification
PTM: Sumoylated on Lys-74 and Lys-698; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter. {ECO:0000269|PubMed:19029252}.
uniprot
proteins
protein_family
CTCF zinc-finger protein family
uniprot
proteins
pubmed
11743158
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15669143
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16614224
uniprot
proteins
pubmed
16949368
uniprot
proteins
pubmed
16951251
uniprot
proteins
pubmed
17329968
uniprot
proteins
pubmed
17952071
uniprot
proteins
pubmed
18614575
uniprot
proteins
pubmed
19029252
uniprot
proteins
pubmed
26276636
uniprot
proteins
pubmed
26527277
uniprot
proteins
pubmed
26961175
uniprot
proteins
pubmed
28388437
uniprot
proteins
pubmed
34856126
uniprot
proteins
pubmed
8649389
uniprot
proteins
sequence_length
736
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000305|PubMed:8649389}. Chromosome {ECO:0000250|UniProtKB:P49711}. Chromosome, centromere {ECO:0000250|UniProtKB:P49711}. Note=May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. {ECO:0000250|UniProtKB:P49711}.
uniprot
proteins