HELQ
entrez
113510
canonical
uniprot
Q8TDG4
canonical
ensembl
ENSG00000163312
resolver
ensp
ENSP00000295488
resolver
ensp
ENSP00000424539
resolver
ensp
ENSP00000780603
resolver
enst
ENST00000510985
resolver
genesymbol
HELQ
resolver
genesymbol_syn
HEL308
resolver
hgnc
HGNC:18536
resolver
uniprot
E3W980
resolver
uniprot
Q05DF9
resolver
uniprot
Q502W9
resolver
uniprot
Q659B8
resolver
uniprot
Q6ZQX4
resolver
uniprot
Q6ZTS4
resolver
uniprot
Q96EX7
resolver
uniprot_entry
E3W980_HUMAN
resolver
uniprot_entry
HELQ_HUMAN
resolver
uniprot_sec
Q05DF9
resolver
uniprot_sec
Q502W9
resolver
uniprot_sec
Q659B8
resolver
uniprot_sec
Q6ZQX4
resolver
uniprot_sec
Q6ZTS4
resolver
uniprot_sec
Q96EX7
resolver
activity_regulation
ACTIVITY REGULATION: ATPase activity is strongly stimulated by single-stranded DNA (PubMed:11751861). Presence of ATP and Mg cofactor are required for helicase activity allowing to unwind duplex oligonucleotides up to 60-70-mer (PubMed:11751861). This helicase activity is stimulated by replication protein A (RPA/RP-A) complex that binds to unwound regions and inhibits re-annealing (PubMed:11751861). {ECO:0000269|PubMed:11751861}.
uniprot
proteins
amino_acid_sequence
MDECGSRIRRRVSLPKRNRPSLGCIFGAPTAAELVPGDEGKEEEEMVAENRRRKTAGVLPVEVQPLLLSDSPECLVLGGGDTNPDLLRHMPTDRGVGDQPNDSEVDMFGDYDSFTENSFIAQVDDLEQKYMQLPEHKKHATDFATENLCSESIKNKLSITTIGNLTELQTDKHTENQSGYEGVTIEPGADLLYDVPSSQAIYFENLQNSSNDLGDHSMKERDWKSSSHNTVNEELPHNCIEQPQQNDESSSKVRTSSDMNRRKSIKDHLKNAMTGNAKAQTPIFSRSKQLKDTLLSEEINVAKKTVESSSNDLGPFYSLPSKVRDLYAQFKGIEKLYEWQHTCLTLNSVQERKNLIYSLPTSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKGRFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMTLAKILYTSKTTQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSKAENGMTFSRLLNYKYSDTLKKMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKFLSKEYLKHKEKEKCEVIKNLKNIGNGNLCPVLKRTIPFGVAYHHSGLTSDERKLLEEAYSTGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQYKQMIGRAGRAGIDTIGESILILQEKDKQQVLELITKPLENCYSHLVQEFTKGIQTLFLSLIGLKIATNLDDIYHFMNGTFFGVQQKVLLKEKSLWEITVESLRYLTEKGLLQKDTIYKSEEEVQYNFHITKLGRASFKGTIDLAYCDILYRDLKKGLEGLVLESLLHLIYLTTPYDLVSQCNPDWMIYFRQFSQLSPAEQNVAAILGVSESFIGKKASGQAIGKKVDKNVVNRLYLSFVLYTLLKETNIWTVSEKFNMPRGYIQNLLTGTASFSSCVLHFCEELEEFWVYRALLVELTKKLTYCVKAELIPLMEVTGVLEGRAKQLYSAGYKSLMHLANANPEVLVRTIDHLSRRQAKQIVSSAKMLLHEKAEALQEEVEELLRLPSDFPGAVASSTDKA
uniprot
proteins
ec_number
5.6.2.4
uniprot
proteins
function
FUNCTION: Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair (PubMed:11751861, PubMed:19995904, PubMed:21398521, PubMed:24005041, PubMed:24005565, PubMed:34316696, PubMed:34937945). Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA) (PubMed:34937945). Possesses both DNA unwinding and annealing activities (PubMed:34937945). Forms a complex with RAD51, stimulating HELQ DNA helicase activity and ability to unwing DNA (PubMed:34937945). Efficiently unwinds substrates containing 3' overhangs or a D-loop (PubMed:21398521, PubMed:34937945). In contrast, interaction with the replication protein A (RPA/RP-A) complex inhibits DNA unwinding by HELQ but strongly stimulates DNA strand annealing (PubMed:34937945). Triggers displacement of RPA from single-stranded DNA to facilitate annealing of complementary sequences (PubMed:34316696, PubMed:34937945). {ECO:0000269|PubMed:11751861, ECO:0000269|PubMed:19995904, ECO:0000269|PubMed:21398521, ECO:0000269|PubMed:24005041, ECO:0000269|PubMed:24005565, ECO:0000269|PubMed:34316696, ECO:0000269|PubMed:34937945}.
uniprot
proteins
mass_dalton
124131
uniprot
proteins
mutagenesis
MUTAGEN 365; /note="K->M: Abolishes ATPase and DNA helicase activity."; /evidence="ECO:0000269|PubMed:11751861, ECO:0000269|PubMed:34937945"; MUTAGEN 463; /note="D->A: Abolished ATPase and DNA helicase activity."; /evidence="ECO:0000269|PubMed:34316696"; MUTAGEN 818..819; /note="YK->SD: Abolished double-stranded DNA-binding."; /evidence="ECO:0000269|PubMed:28738244
uniprot
proteins
protein_family
Helicase family
uniprot
proteins
protein_family
SKI2 subfamily
uniprot
proteins
pubmed
11751861
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15815621
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
17974005
uniprot
proteins
pubmed
19995904
uniprot
proteins
pubmed
21398521
uniprot
proteins
pubmed
24005041
uniprot
proteins
pubmed
24005565
uniprot
proteins
pubmed
28738244
uniprot
proteins
pubmed
34316696
uniprot
proteins
pubmed
34937945
uniprot
proteins
sequence_length
1101
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21398521}. Chromosome {ECO:0000269|PubMed:21398521, ECO:0000269|PubMed:34937945}. Note=Localizes to sites of DNA damage; localizes to damaged replication forks. {ECO:0000269|PubMed:21398521, ECO:0000269|PubMed:34937945}.
uniprot
proteins