Shtn1
entrez
292139
canonical
uniprot
A0MZ67
canonical
ensembl
ENSRNOG00000018350
resolver
ensp
ENSRNOP00000061843
resolver
ensp
ENSRNOP00000074944
resolver
genesymbol
Shtn1
resolver
uniprot
A0MZ64
resolver
uniprot_entry
SHOT1_RAT
resolver
uniprot_sec
A0MZ64
resolver
amino_acid_sequence
MNSSDEEKQLQLITSLKEQAIGEYEDLRAENQKTKETCDKIRQERDEAVKKLEEFQKISHMVIEEVNFMQNHLEIEKTCRESAEALATKLNKENKTLKRISMLYMAKLGPDVITEEINIDDDDPGTDTDAAAETCVSVQCQKQIKELRDQIVSVQEEKKVLAIELESLKSKLGEVMEEVNKVKQEKAVLNSEVLEQRKVLEKCNRVSVLAVEEYEELQVNLELEKDLRKKAESFAQEMFIEQNKLKRQSHLLLQSSLPDQQLLKALDENAKLIQQLEEERIQHQQKVKELEERLENEALHKEIHNLRQQLELLEDDKRELEQKYQSSEEKARNLKHSVDELQKRVNQSENSVPPPPPPPPPLPPPPPNPIRSLMSMIRKRSHPSGGSTKKEKATQPETAEEVTDLKRQAVEEMMDRIKKGVHLRPVNQTARPKAKPDSLKGSESAVDELKGILGTLNKSTSSRSLKSLGPENSETELERILRRRKLTAEADSSSPTGILATSESKSMPVLGSVSSVTKSALNKKTLEAEFNNPCPLTPEPGEGPRKLEGCTNSKVTFQPPSKGGYRRKCVGSENQSEPVVVLDPVSTHEPQTKDQAAEKDPTQCKEEERGETQPEFKEDSSGGKTGETDSSNC
uniprot
proteins
function
FUNCTION: Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth (PubMed:17030985, PubMed:17439943, PubMed:18519736, PubMed:20664640). Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth (PubMed:18519736, PubMed:23453953). Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone (PubMed:17030985). Also plays a role in regenerative neurite outgrowth (PubMed:20664640). In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons (By similarity). {ECO:0000250|UniProtKB:Q8K2Q9, ECO:0000269|PubMed:17030985, ECO:0000269|PubMed:17439943, ECO:0000269|PubMed:18519736, ECO:0000269|PubMed:20664640, ECO:0000269|PubMed:23453953}.
uniprot
proteins
mass_dalton
71446
uniprot
proteins
mutagenesis
MUTAGEN 101; /note="S->A: Inhibits F-actin retrograde flow at the peripheral region of growth cones; when associated with A-249."; /evidence="ECO:0000269|PubMed:23453953"; MUTAGEN 101; /note="S->D: Does not inhibit F-actin retrograde flow at the peripheral region of growth cones; when associated with D-249."; /evidence="ECO:0000269|PubMed:23453953"; MUTAGEN 249; /note="S->A: Inhibits F-actin retrograde flow at the peripheral region of growth cones; when associated with A-101."; /evidence="ECO:0000269|PubMed:23453953"; MUTAGEN 249; /note="S->D: Does not inhibit F-actin retrograde flow at the peripheral region of growth cones; when associated with D-101."; /evidence="ECO:0000269|PubMed:23453953
uniprot
proteins
post_translational_modification
PTM: Phosphorylated on Ser-101 and Ser-249 by PAK1 through a CDC42- and RAC1-dependent signaling pathway, which enhances its association with F-actin retrograde flow in filopodia and lamellipodia of axonal growth cones (PubMed:23453953). Phosphorylation on Ser-101 and Ser-249 is increased by netrin-1 (PubMed:23453953). {ECO:0000269|PubMed:23453953}.
uniprot
proteins
protein_family
Shootin family
uniprot
proteins
pubmed
17030985
uniprot
proteins
pubmed
17439943
uniprot
proteins
pubmed
18519736
uniprot
proteins
pubmed
20664640
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
23408951
uniprot
proteins
pubmed
23453953
uniprot
proteins
sequence_length
633
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Perikaryon {ECO:0000269|PubMed:17030985, ECO:0000269|PubMed:20664640}. Cell projection, axon {ECO:0000269|PubMed:17030985, ECO:0000269|PubMed:20664640}. Cell projection, growth cone {ECO:0000269|PubMed:17030985, ECO:0000269|PubMed:18519736, ECO:0000269|PubMed:20664640, ECO:0000269|PubMed:23453953}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q8K2Q9}. Cell projection, filopodium {ECO:0000269|PubMed:18519736, ECO:0000269|PubMed:23453953}. Cell projection, lamellipodium {ECO:0000269|PubMed:18519736, ECO:0000269|PubMed:23453953}. Note=Localizes in multiple growth cones at neurite tips before the neuronal symmetry-breaking step (PubMed:23453953). Accumulates in growth cones of a single nascent axon in a neurite length-dependent manner during the neuronal symmetry-breaking step; when absent from the nascent axon's siblings, probably due to competitive transport, prevents the formation of surplus axons (PubMed:17030985, PubMed:23453953). Transported anterogradely from the soma to the axon growth cone in an actin and myosin-dependent manner and passively diffuses back to the cell bodies (PubMed:23453953). Colocalized with L1CAM in close apposition with actin filaments in filopodia and lamellipodia of axonal growth cones in hippocampal neurons (PubMed:18519736). Exhibits retrograde movements in filopodia and lamellopodia of axonal growth cones (PubMed:18519736). Colocalized with KIF20B along microtubules to the tip of the growing cone in primary hippocampal neurons. Recruited to the growth cone of developing axon in a KIF20B- and microtubule-dependent manner (By similarity). {ECO:0000250|UniProtKB:Q8K2Q9, ECO:0000269|PubMed:17030985, ECO:0000269|PubMed:18519736, ECO:0000269|PubMed:23453953}.
uniprot
proteins