P35281
uniprot
P35281
canonical
activity_regulation
ACTIVITY REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) DENND4C and RABIF which promote the exchange of bound GDP for free GTP. Regulated by GTPase activating proteins (GAPs) including TBC1D21 which increase the GTP hydrolysis activity. Inhibited by GDP dissociation inhibitors GDI1 and GDI2 which prevent Rab-GDP dissociation. {ECO:0000250|UniProtKB:P61026}.
uniprot
proteins
amino_acid_sequence
MAKKTYDLLFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIEIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDQHANEDVERMLLRNKCDMDHKRVVPKGKGEQIAREHRIRFFETSAKANINIEKAFLTLPEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC
uniprot
proteins
ec_number
3.6.5.2
uniprot
proteins
function
FUNCTION: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:24662485). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB10 is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane (By similarity). Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane (By similarity). In parallel, RAB10 regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response (By similarity). Also plays a specific role in asymmetric protein transport to the plasma membrane (By similarity). In neurons, involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane (PubMed:21856246). In epithelial cells, regulates transport from the Golgi to the basolateral membrane (By similarity). May play a role in the basolateral recycling pathway and in phagosome maturation (By similarity). May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Together with LRRK2, RAB8A, and RILPL1, regulates ciliogenesis. When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis. Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route. Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation where it promotes the extracellular release of lysosomal content through EHBP1 and EHNP1L1 effector proteins (By similarity). {ECO:0000250|UniProtKB:P24409, ECO:0000250|UniProtKB:P61026, ECO:0000250|UniProtKB:P61027, ECO:0000269|PubMed:21856246, ECO:0000269|PubMed:24662485}.
uniprot
proteins
mass_dalton
22858
uniprot
proteins
post_translational_modification
PTM: Phosphorylation of Thr-73 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM and RAB GDP dissociation inhibitors GDI1 and GDI2 (By similarity). Phosphorylation of Thr-73 by LRRK2 is stimulated by RAB29 and RAB32 (By similarity). Phosphorylation by LRRK2 is required for localization to stressed lysosomes (By similarity). {ECO:0000250|UniProtKB:P61026}.
uniprot
proteins
protein_family
Rab family
uniprot
proteins
protein_family
Small GTPase superfamily
uniprot
proteins
pubmed
1313420
uniprot
proteins
pubmed
20576682
uniprot
proteins
pubmed
21856246
uniprot
proteins
pubmed
24662485
uniprot
proteins
sequence_length
200
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000305}; Lipid-anchor {ECO:0000305|PubMed:20576682}; Cytoplasmic side {ECO:0000305|PubMed:20576682}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:P24409}. Endosome membrane {ECO:0000250|UniProtKB:P61026}. Recycling endosome membrane {ECO:0000250|UniProtKB:P24409}. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:P24409}. Cell projection, cilium {ECO:0000269|PubMed:20576682}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P61027}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P61027}. Lysosome {ECO:0000250|UniProtKB:P61027}. Note=Associates with SLC2A4/GLUT4 storage vesicles (By similarity). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (By similarity). Localizes to enlarged lysosomes through LRRK2 phosphorylation (By similarity). {ECO:0000250|UniProtKB:P24409, ECO:0000250|UniProtKB:P61026, ECO:0000269|PubMed:20576682}.
uniprot
proteins