O00370
uniprot
O00370
canonical
amino_acid_sequence
MTGSNSHITILTLNVNGLNSPIKRHRLASWIKSQDPSVCCIQETHLTCRDTHRLKIKGWRKIYQANGKQKKAGVAILVSDKTDFKPTKIKRDKEGHYIMVKGSIQQEELTILNIYAPNTGAPRFIKQVLSDLQRDLDSHTLIMGDFNTPLSILDRSTRQKVNKDTQELNSALHQTDLIDIYRTLHPKSTEYTFFSAPHHTYSKIDHIVGSKALLSKCKRTEIITNYLSDHSAIKLELRIKNLTQSRSTTWKLNNLLLNDYWVHNEMKAEIKMFFETNENKDTTYQNLWDAFKAVCRGKFIALNAYKRKQERSKIDTLTSQLKELEKQEQTHSKASRRQEITKIRAELKEIETQKTLQKINESRSWFFERINKIDRPLARLIKKKREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLPRLNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEKEIKGIQLGKEEVKLSLFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYNNNRQTESQIMGELPFTIASKRIKYLGIQLTRDVKDLFKENYKPLLKEIKEDTNKWKNIPCSWVGRINIVKMAILPKVIYRFNAIPIKLPMTFFTELEKTTLKFIWNQKRARIAKSILSQKNKAGGITLPDFKLYYKATVTKTAWYWYQNRDIDQWNRTEPSEIMPHIYNYLIFDKPEKNKQWGKDSLLNKWCWENWLAICRKLKLDPFLTPYTKINSRWIKDLNVKPKTIKTLEENLGITIQDIGVGKDFMSKTPKAMATKDKIDKWDLIKLKSFCTAKETTIRVNRQPTTWEKIFATYSSDKGLISRIYNELKQIYKKKTNNPIKKWAKDMNRHFSKEDIYAAKKHMKKCSSSLAIREMQIKTTMRYHLTPVRMAIIKKSGNNRCWRGCGEIGTLVHCWWDCKLVQPLWKSVWRFLRDLELEIPFDPAIPLLGIYPKDYKSCCYKDTCTRMFIAALFTIAKTWNQPNCPTMIDWIKKMWHIYTMEYYAAIKNDEFISFVGTWMKLETIILSKLSQEQKTKHRIFSLIGGN
uniprot
proteins
ec_number
2.7.7.49
uniprot
proteins
ec_number
3.1.21.-
uniprot
proteins
function
FUNCTION: Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition (PubMed:38096901, PubMed:38096902, PubMed:7516468, PubMed:9140393). Selectively binds and reversely transcribes RNA with a poly(A) tail consisting of at least 20 adenosines (PubMed:38096901). Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA (PubMed:17626046, PubMed:34554261, PubMed:38096901, PubMed:38096902, PubMed:8945517). Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to the sites of LINE-1 integration in the genome (PubMed:8945517). Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition (PubMed:17626046, PubMed:34554261). Unlike related endonucleases, does not bend the DNA helix but causes compression near the cleavage site (PubMed:34554261). {ECO:0000269|PubMed:17626046, ECO:0000269|PubMed:34554261, ECO:0000269|PubMed:38096901, ECO:0000269|PubMed:38096902, ECO:0000269|PubMed:7516468, ECO:0000269|PubMed:8945517, ECO:0000269|PubMed:9140393}.
uniprot
proteins
mass_dalton
149012
uniprot
proteins
mutagenesis
MUTAGEN 14; /note="N->A: Loss of endonuclease activity and reduced transposition efficiency."; /evidence="ECO:0000269|PubMed:8945517"; MUTAGEN 43; /note="E->A: Loss of endonuclease activity."; /evidence="ECO:0000269|PubMed:8945517"; MUTAGEN 145; /note="D->A: Loss of endonuclease activity and reduced transposition efficiency. Reduced transposition efficiency; when associated with A-147."; /evidence="ECO:0000269|PubMed:17626046, ECO:0000269|PubMed:34554261, ECO:0000269|PubMed:8945517"; MUTAGEN 147; /note="N->A: Reduced transposition efficiency; when associated with A-145."; /evidence="ECO:0000269|PubMed:17626046"; MUTAGEN 155; /note="R->A: Reduced DNA nicking activity and reduced transposition efficiency."; /evidence="ECO:0000269|PubMed:17626046"; MUTAGEN 192; /note="T->V: Reduced transposition efficiency."; /evidence="ECO:0000269|PubMed:17626046"; MUTAGEN 202; /note="S->A: Reduced DNA nicking activity and reduced transposition efficiency."; /evidence="ECO:0000269|PubMed:17626046"; MUTAGEN 204; /note="I->Y: Reduced DNA nicking activity and reduced transposition efficiency."; /evidence="ECO:0000269|PubMed:17626046"; MUTAGEN 205; /note="D->G: Loss of endonuclease activity and reduced transposition efficiency."; /evidence="ECO:0000269|PubMed:8945517"; MUTAGEN 226; /note="Y->K: Increased endonuclease activity."; /evidence="ECO:0000269|PubMed:34554261"; MUTAGEN 230; /note="H->A: Loss of endonuclease activity and reduced transposition efficiency."; /evidence="ECO:0000269|PubMed:17626046, ECO:0000269|PubMed:8945517"; MUTAGEN 701; /note="A->M: Reduces transposition efficiency."; /evidence="ECO:0000269|PubMed:38096902"; MUTAGEN 703; /note="D->Y: Reduced transposition efficiency."; /evidence="ECO:0000269|PubMed:8945517"; MUTAGEN 1130; /note="C->R: No effect on binding to RNA; when associated with R-1134, R-1143 and R-1147."; /evidence="ECO:0000269|PubMed:24251107"; MUTAGEN 1134; /note="C->R: No effect on binding to RNA; when associated with R-1130, R-1143 and R-1147."; /evidence="ECO:0000269|PubMed:24251107"; MUTAGEN 1143; /note="C->R: No effect on binding to RNA; when associated with R-1130, R-1134 and R-1147."; /evidence="ECO:0000269|PubMed:24251107"; MUTAGEN 1147; /note="C->R: No effect on binding to RNA; when associated with R-1130, R-1134 and R-1143."; /evidence="ECO:0000269|PubMed:24251107
uniprot
proteins
pubmed
15274918
uniprot
proteins
pubmed
17626046
uniprot
proteins
pubmed
24251107
uniprot
proteins
pubmed
34554261
uniprot
proteins
pubmed
35760782
uniprot
proteins
pubmed
36639728
uniprot
proteins
pubmed
38096901
uniprot
proteins
pubmed
38096902
uniprot
proteins
pubmed
7516468
uniprot
proteins
pubmed
8945517
uniprot
proteins
pubmed
9140393
uniprot
proteins
sequence_length
1275
uniprot
proteins