HCST
entrez
10870
canonical
uniprot
Q9UBK5
canonical
ensembl
ENSG00000126264
resolver
ensp
ENSP00000246551
resolver
ensp
ENSP00000400516
resolver
ensp
ENSP00000678830
resolver
ensp
ENSP00000678831
resolver
genesymbol
HCST
resolver
genesymbol_syn
DAP10
resolver
genesymbol_syn
KAP10
resolver
genesymbol_syn
PIK3AP
resolver
hgnc
HGNC:16977
resolver
uniprot
Q9UBS1
resolver
uniprot
Q9Y3Y0
resolver
uniprot_entry
HCST_HUMAN
resolver
uniprot_sec
Q9UBS1
resolver
uniprot_sec
Q9Y3Y0
resolver
amino_acid_sequence
MIHLGHILFLLLLPVAAAQTTPGERSSLPAFYPGTSGSCSGCGSLSLPLLAGLVAADAVASLLIVGAVFLCARPRRSPAQEDGKVYINMPGRG
uniprot
proteins
function
FUNCTION: Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as a docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells. {ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:10528161, ECO:0000269|PubMed:11015446, ECO:0000269|PubMed:11777960, ECO:0000269|PubMed:12740575, ECO:0000269|PubMed:12740576, ECO:0000269|PubMed:16002667, ECO:0000269|PubMed:16339517, ECO:0000269|PubMed:16582911, ECO:0000269|PubMed:18097042}.
uniprot
proteins
mass_dalton
9489
uniprot
proteins
mutagenesis
MUTAGEN 57; /note="D->A: Abolishes stable interaction with KLRK1."; /evidence="ECO:0000269|PubMed:10426994"; MUTAGEN 88; /note="N->Q: Abrogates cell killing and interaction with GRB2. No effect on interaction with PIK3R1."; /evidence="ECO:0000269|PubMed:16582911"; MUTAGEN 89; /note="M->Q: Abrogates cell killing and interaction with PIK3R1. No effect on interaction with GRB2."; /evidence="ECO:0000269|PubMed:16582911
uniprot
proteins
post_translational_modification
PTM: Phosphorylated; PIK3R1 and GRB2 associate specifically with tyrosine-phosphorylated HCST. {ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:10528161, ECO:0000269|PubMed:16582911}.; PTM: O-glycosylated. {ECO:0000269|PubMed:10426994}.
uniprot
proteins
protein_family
DAP10 family
uniprot
proteins
pubmed
10426994
uniprot
proteins
pubmed
10528161
uniprot
proteins
pubmed
11015446
uniprot
proteins
pubmed
11398969
uniprot
proteins
pubmed
11777960
uniprot
proteins
pubmed
12740575
uniprot
proteins
pubmed
12740576
uniprot
proteins
pubmed
12975309
uniprot
proteins
pubmed
15294961
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15894612
uniprot
proteins
pubmed
16002667
uniprot
proteins
pubmed
16339517
uniprot
proteins
pubmed
16424177
uniprot
proteins
pubmed
16582911
uniprot
proteins
pubmed
17974005
uniprot
proteins
pubmed
18097042
uniprot
proteins
pubmed
26221034
uniprot
proteins
sequence_length
93
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:10426994}; Single-pass type I membrane protein {ECO:0000269|PubMed:10426994}.
uniprot
proteins
transmembrane_region
TRANSMEM 49..69; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins