Nfs1
entrez
18041
canonical
uniprot
Q9Z1J3
canonical
ensembl
ENSMUSG00000027618
resolver
ensp
ENSMUSP00000029147
resolver
ensp
ENSMUSP00000105229
resolver
ensp
ENSMUSP00000248495
resolver
ensp
ENSMUSP00000248521
resolver
enst
ENSMUST00000029147
resolver
enst
ENSMUST00000109600
resolver
genesymbol
Nfs1
resolver
genesymbol_syn
Nifs
resolver
uniprot
A6QRH3
resolver
uniprot
Q8C6I5
resolver
uniprot
Q9CTB2
resolver
uniprot_entry
A6QRH3_MOUSE
resolver
uniprot_entry
NFS1_MOUSE
resolver
uniprot_entry
Q9CTB2_MOUSE
resolver
uniprot_sec
Q8C6I5
resolver
activity_regulation
ACTIVITY REGULATION: Active only in complex with LYRM4. {ECO:0000250|UniProtKB:Q9Y697}.
uniprot
proteins
amino_acid_sequence
MVGSVAGNMLLRAAWRRASLAATSLALGRSSVPTRGLRLRVVDHGPHSPVHSEAEAVLRPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQNIMKNLPDVVMNGDPKQHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH
uniprot
proteins
ec_number
2.8.1.7
uniprot
proteins
function
FUNCTION: Mitochondrial cysteine desulfurase, of the core iron-sulfur cluster (ISC) assembly complex, that catalyzes the desulfuration of L-cysteine to L-alanine, as component of the cysteine desulfurase complex, leading to the formation of a cysteine persulfide intermediate at the active site cysteine residue and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed:25597503). The persulfide is then transferred on the flexible Cys loop from the catalytic site of NFS1 to the surface of NFS1 (By similarity). After the NFS1-linked persulfide sulfur is transferred to one of the conserved Cys residues of the scaffold, a reaction assisted by FXN (PubMed:25597503). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity). {ECO:0000250|UniProtKB:Q9H1K1, ECO:0000250|UniProtKB:Q9Y697, ECO:0000269|PubMed:25597503}.
uniprot
proteins
mass_dalton
50570
uniprot
proteins
post_translational_modification
PTM: N-gluconoylated. {ECO:0000269|PubMed:25597503}.; PTM: Cysteine persulfide intermediate is reduced by thiol-containing molecules like glutathione and L-cysteine (PubMed:25597503). Persulfide reduction is a rate-limiting step of cysteine desulfurase catalytic cycle (PubMed:25597503). {ECO:0000269|PubMed:25597503}.
uniprot
proteins
protein_family
Class-V pyridoxal-phosphate-dependent aminotransferase family
uniprot
proteins
protein_family
NifS/IscS subfamily
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21298097
uniprot
proteins
pubmed
25597503
uniprot
proteins
pubmed
9738949
uniprot
proteins
sequence_length
459
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:9738949}.
uniprot
proteins