Gcnt1
entrez
14537
canonical
uniprot
Q09324
canonical
ensembl
ENSMUSG00000038843
resolver
ensp
ENSMUSP00000127835
resolver
ensp
ENSMUSP00000133935
resolver
ensp
ENSMUSP00000157544
resolver
ensp
ENSMUSP00000157681
resolver
ensp
ENSMUSP00000263565
resolver
ensp
ENSMUSP00000326026
resolver
enst
ENSMUST00000169897
resolver
enst
ENSMUST00000174236
resolver
enst
ENSMUST00000235184
resolver
enst
ENSMUST00000236139
resolver
genesymbol
Gcnt1
resolver
uniprot
O35981
resolver
uniprot
Q8BRB2
resolver
uniprot_entry
GCNT1_MOUSE
resolver
uniprot_sec
O35981
resolver
uniprot_sec
Q8BRB2
resolver
activity_regulation
ACTIVITY REGULATION: Inactivated by thiol-reactive agents. Inhibited by free UDP. {ECO:0000269|PubMed:12954635}.
uniprot
proteins
amino_acid_sequence
MLRNLFRRRLFSCPTKYYFMLLVLSLITFSVLRIHQKPEFFSVRHLELAGDDPYSNVNCTKILQGDPEEIQKVKLEILTVQFKKRPRRTPHDYINMTRDCASFIRTRKYIVEPLTKEEVGFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDRKAEESFLAAVQGIASCFDNVFVASQLESVVYASWSRVKADLNCMKDLYRMNANWKYLINLCGMDFPIKTNLEIVRKLKCSTGENNLETEKMPPNKEERWKKRYTVVDGKLTNTGIVKAPPPLKTPLFSGSAYFVVTREYVGYVLENENIQKLMEWAQDTYSPDEFLWATIQRIPEVPGSFPSSNKYDLSDMNAIARFVKWQYFEGHVSNGAPYPPCSGVHVRSVCVFGAGDLSWMLRQHHLFANKFDMDVDPFAIQCLDEHLRHKALENLEH
uniprot
proteins
ec_number
2.4.1.102
uniprot
proteins
function
FUNCTION: Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine (GlcNAc) moiety in beta1-6 linkage from UDP-GlcNAc onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan. The catalysis is metal ion-independent and occurs with inversion of the anomeric configuration of sugar donor (PubMed:12954635, PubMed:22056345, PubMed:9881978). Selectively involved in synthesis of mucin-type core 2 O-glycans that serve as scaffolds for the display of selectin ligand sialyl Lewis X epitope by myeloid cells, with an impact on homeostasis and recruitment to inflammatory sites (PubMed:9881978). Can also act on glycolipid substrates. Transfers GlcNAc moiety to GalGb4Cer globosides in a reaction step to the synthesis of stage-specific embryonic antigen 1 (SSEA-1) determinant (PubMed:7983056). Can use Galbeta1-3GalNAcalpha1-R and Galbeta1-3GalNAcbeta1-R oligosaccharide derivatives as acceptor substrates (PubMed:7983056). {ECO:0000269|PubMed:12954635, ECO:0000269|PubMed:22056345, ECO:0000269|PubMed:7983056, ECO:0000269|PubMed:9881978}.
uniprot
proteins
mass_dalton
49839
uniprot
proteins
mutagenesis
MUTAGEN 217; /note="C->S: Protects from inactivation caused by air oxidation or thiol-reactive agents. Reduces the affinity for UDP-GlcNAc; when associated with A-378. Abolishes binding to UDP-GlcNAc; when associated with A-401. Loss of catalytic activity; when associated with A-378 and A-401."; /evidence="ECO:0000269|PubMed:12954635, ECO:0000269|PubMed:22056345"; MUTAGEN 378; /note="R->A: Loss of catalytic activity; when associated with S-217 and A-401. Reduces the affinity for UDP-GlcNAc; when associated with S-217."; /evidence="ECO:0000269|PubMed:22056345"; MUTAGEN 401; /note="K->A: Loss of catalytic activity; when associated with S-217 and A-378. Abolishes binding to UDP-GlcNAc; when associated with S-217."; /evidence="ECO:0000269|PubMed:22056345
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein glycosylation. {ECO:0000269|PubMed:9881978}.; PATHWAY: Glycolipid biosynthesis. {ECO:0000269|PubMed:7983056}.
uniprot
proteins
post_translational_modification
PTM: N-glycosylated. {ECO:0000269|PubMed:12954635}.
uniprot
proteins
protein_family
Glycosyltransferase 14 family
uniprot
proteins
pubmed
12954635
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16829524
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22056345
uniprot
proteins
pubmed
7983056
uniprot
proteins
pubmed
9341170
uniprot
proteins
pubmed
9881978
uniprot
proteins
sequence_length
428
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q02742}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q02742}. Note=Also detected in the trans-Golgi network. {ECO:0000250|UniProtKB:Q02742}.
uniprot
proteins
transmembrane_region
TRANSMEM 10..32; /note="Helical; Signal-anchor for type II membrane protein"; /evidence="ECO:0000255
uniprot
proteins