RTCB
entrez
51493
canonical
uniprot
Q9Y3I0
canonical
ensembl
ENSG00000100220
resolver
ensp
ENSP00000216038
resolver
enst
ENST00000216038
resolver
genesymbol
RTCB
resolver
genesymbol_syn
C22orf28
resolver
hgnc
HGNC:26935
resolver
uniprot
B2R6A8
resolver
uniprot
Q6IAI0
resolver
uniprot
Q9BWL4
resolver
uniprot
Q9NTH1
resolver
uniprot
Q9P037
resolver
uniprot
Q9P0J3
resolver
uniprot_entry
RTCB_HUMAN
resolver
uniprot_sec
B2R6A8
resolver
uniprot_sec
Q6IAI0
resolver
uniprot_sec
Q9BWL4
resolver
uniprot_sec
Q9NTH1
resolver
uniprot_sec
Q9P037
resolver
uniprot_sec
Q9P0J3
resolver
activity_regulation
ACTIVITY REGULATION: ZBTB8OS/Archease stimulates the activity of the tRNA ligase complex by participating to RTCB active site guanylylation reaction (PubMed:24870230). While Mg(2+), Ca(2+) and to a lesser extent Co(2+) show low levels of covalent incorporation of GMP the strongest guanylylation occurs in presence of Mn(2+) ions (PubMed:38493148). {ECO:0000269|PubMed:24870230, ECO:0000269|PubMed:38493148}.
uniprot
proteins
amino_acid_sequence
MSRSYNDELQFLEKINKNCWRIKKGFVPNMQVEGVFYVNDALEKLMFEELRNACRGGGVGGFLPAMKQIGNVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDPEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVKEQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSARAKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGISKKAIKLRPIAVIKG
uniprot
proteins
ec_number
6.5.1.8
uniprot
proteins
function
FUNCTION: 3'-5' RNA ligase, catalytic subunit of the tRNA-splicing ligase complex (tRNA-LC), which is involved in the enzyme-dependent maturation of intron-containing pre-tRNAs. Functions downstream of the tRNA-splicing endonuclease that removes introns, ligating the two generated halves via phosphodiester bond formation (PubMed:40069351). The ligation reaction, which requires guanosine triphosphate (GTP) and Mn(2+), proceeds through three metal-dependent steps. The first step requires ZBTB8OS/Archease and involves the guanylylation of RTCB at its active site histidine, forming a covalent GMP-histidine intermediate. Before the second step, RTCB also hydrolyzes the 2',3'-cyclic phosphate (cP) at the 3' end of the 5' tRNA exon, typically generated by the tRNA-splicing endonuclease, producing a 3' phosphate. The covalently bound GMP is then transferred to this 3' phosphate to form an RNA(3')-P-P-(5')G intermediate. In the final step, the 5'-OH of the 3' exon attacks the activated 3' end of the 5' exon, forming a 3'-5' phosphodiester bond and releasing GMP (PubMed:24870230, PubMed:38493148). RTCB also functions in non-canonical, spliceosome-independent, cytoplasmic splicing of XBP1 mRNAs during the unfolded protein response (UPR). Upon endoplasmic reticulum (ER) stress, the endoribonuclease IRE1/ERN1 excises a short intron, generating free exon ends that are aligned by RNA-intrinsic, zipper-like stem-loop structures. These exon ends are then recognized and ligated by RTCB. This splicing event yields the active XBP1s transcription factor, which induces genes required to resolve protein folding defects in the endoplasmic reticulum (PubMed:25378478, PubMed:26483401, PubMed:38493148). {ECO:0000269|PubMed:24870230, ECO:0000269|PubMed:25378478, ECO:0000269|PubMed:26483401, ECO:0000269|PubMed:38493148, ECO:0000269|PubMed:40069351}.
uniprot
proteins
mass_dalton
55210
uniprot
proteins
mutagenesis
MUTAGEN 119; /note="D->A: Loss of RNA ligase (GTP) activity."; /evidence="ECO:0000269|PubMed:38493148"; MUTAGEN 122; /note="C->A: Loss of RNA ligase (GTP) activity."; /evidence="ECO:0000269|PubMed:21311021, ECO:0000269|PubMed:38493148"; MUTAGEN 227; /note="H->A: Loss of RNA ligase (GTP) activity."; /evidence="ECO:0000269|PubMed:38493148"; MUTAGEN 259; /note="H->A: Loss of RNA ligase (GTP) activity."; /evidence="ECO:0000269|PubMed:38493148"; MUTAGEN 353; /note="H->A: Loss of RNA ligase (GTP) activity."; /evidence="ECO:0000269|PubMed:38493148
uniprot
proteins
protein_family
RtcB family
uniprot
proteins
pubmed
10591208
uniprot
proteins
pubmed
10931946
uniprot
proteins
pubmed
11042152
uniprot
proteins
pubmed
12529303
uniprot
proteins
pubmed
14654843
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15312650
uniprot
proteins
pubmed
15461802
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16236267
uniprot
proteins
pubmed
17974005
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21311021
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
24608264
uniprot
proteins
pubmed
24870230
uniprot
proteins
pubmed
25378478
uniprot
proteins
pubmed
25944712
uniprot
proteins
pubmed
26483401
uniprot
proteins
pubmed
28112733
uniprot
proteins
pubmed
38493148
uniprot
proteins
pubmed
40069351
uniprot
proteins
sequence_length
505
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24608264, ECO:0000269|PubMed:25378478}. Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03144, ECO:0000269|PubMed:24608264, ECO:0000269|PubMed:25378478}. Note=Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low. {ECO:0000269|PubMed:24608264}.
uniprot
proteins