ALKBH3
entrez
221120
canonical
uniprot
Q96Q83
canonical
ensembl
ENSG00000166199
resolver
ensp
ENSP00000302232
resolver
genesymbol
ALKBH3
resolver
genesymbol_syn
ABH3
resolver
genesymbol_syn
DEPC1
resolver
hgnc
HGNC:30141
resolver
uniprot
A6NDJ1
resolver
uniprot
Q3SYI0
resolver
uniprot
Q6NX57
resolver
uniprot
Q96BU8
resolver
uniprot_entry
ALKB3_HUMAN
resolver
uniprot_sec
A6NDJ1
resolver
uniprot_sec
Q3SYI0
resolver
uniprot_sec
Q6NX57
resolver
uniprot_sec
Q96BU8
resolver
activity_regulation
ACTIVITY REGULATION: Activated by ascorbate. {ECO:0000269|PubMed:12486230}.
uniprot
proteins
amino_acid_sequence
MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRVIDREGVYEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDITYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPDPRGAPW
uniprot
proteins
ec_number
1.14.11.33
uniprot
proteins
ec_number
1.14.11.54
uniprot
proteins
function
FUNCTION: Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:20714506). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA (PubMed:25797601). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:16858410, PubMed:26863196, PubMed:26863410). Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:16858410, PubMed:22055184). {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:16858410, ECO:0000269|PubMed:22055184, ECO:0000269|PubMed:25797601, ECO:0000269|PubMed:25944111, ECO:0000269|PubMed:26863196, ECO:0000269|PubMed:26863410}.
uniprot
proteins
mass_dalton
33375
uniprot
proteins
mutagenesis
MUTAGEN 122..124; /note="RED->VGF: Acquires the capacity to efficiently repair N1-methyladenine adduct in dsDNA."; /evidence="ECO:0000269|PubMed:20714506"; MUTAGEN 122; /note="R->A: Decreases activity towards ssDNA by 25%. Loss of activity towards dsDNA."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 123; /note="E->A: Strongly increases activity towards dsDNA, possibly by facilitating access to the active site."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 131; /note="R->A: Loss of activity."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 177; /note="L->A,N: Loss of activity against N1-methyladenine."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 177; /note="L->E,Q: Loss of activity."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 177; /note="L->I: Decreases activity against N1-methyladenine."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 177; /note="L->M: No effect."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 179; /note="N->A: Decreases activity by about 60%."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 181; /note="Y->A: Strong decrease of activity."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 189; /note="D->A: Strongly increases activity towards dsDNA, possibly by facilitating access to the active site."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 191; /note="H->A: Loss of activity."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 193; /note="D->A: Loss of activity."; /evidence="ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:26863196"; MUTAGEN 257; /note="H->A: Decreases activity by about 65%."; /evidence="ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:22055184"; MUTAGEN 269; /note="R->A: Strong decrease of activity."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 271; /note="N->A: No effect."; /evidence="ECO:0000269|PubMed:16858410"; MUTAGEN 275; /note="R->A: Loss of activity."; /evidence="ECO:0000269|PubMed:16858410
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination. OTUD4 promotes USP7 and USP9X-dependent deubiquitination of 'Lys-48'-polyubiquitinated ALKBH3 promoting the repair of alkylated DNA lesions. {ECO:0000269|PubMed:25944111}.
uniprot
proteins
protein_family
AlkB family
uniprot
proteins
pubmed
12486230
uniprot
proteins
pubmed
12594517
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16174769
uniprot
proteins
pubmed
16554811
uniprot
proteins
pubmed
16858410
uniprot
proteins
pubmed
17979886
uniprot
proteins
pubmed
20714506
uniprot
proteins
pubmed
22055184
uniprot
proteins
pubmed
25797601
uniprot
proteins
pubmed
25944111
uniprot
proteins
pubmed
26863196
uniprot
proteins
pubmed
26863410
uniprot
proteins
pubmed
29144457
uniprot
proteins
sequence_length
286
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:17979886, ECO:0000269|PubMed:29144457}. Cytoplasm {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:17979886}. Note=Colocalizes with ASCC2 and ASCC3 in nuclear foci when cells have been exposed to alkylating agents that cause DNA damage (PubMed:29144457). Predominantly localizes to the nucleus. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:17979886, ECO:0000269|PubMed:29144457}.
uniprot
proteins