CLSPN
entrez
63967
canonical
uniprot
Q9HAW4
canonical
ensembl
ENSG00000092853
resolver
ensp
ENSP00000251195
resolver
ensp
ENSP00000312995
resolver
ensp
ENSP00000362317
resolver
genesymbol
CLSPN
resolver
hgnc
HGNC:19715
resolver
uniprot
A6NFL4
resolver
uniprot
Q1RMC6
resolver
uniprot
Q2KHM3
resolver
uniprot
Q5VYG0
resolver
uniprot
Q6P6H5
resolver
uniprot
Q8IWI1
resolver
uniprot_entry
CLSPN_HUMAN
resolver
uniprot_sec
A6NFL4
resolver
uniprot_sec
Q1RMC6
resolver
uniprot_sec
Q2KHM3
resolver
uniprot_sec
Q5VYG0
resolver
uniprot_sec
Q6P6H5
resolver
uniprot_sec
Q8IWI1
resolver
amino_acid_sequence
MTGEVGSEVHLEINDPNVISQEEADSPSDSGQGSYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSETEDTNASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSLYQENLEAQVKPCLELSLQSGNSTDFTTDRKSSKKHIHDKEGTAGKAKVKSKRRLEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLVDKDLFETGLEDENNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSKGTTRKERKAARLSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFKRKPRPTCHGNAMALLKSSKYQSSHHKEIIDTANTTEMNSDHHSKGSEQTTGAENEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIRPSPGDSSVLQQESNFLGNNHSEECQVGGLVAFEPHALEGEGPQNPEETDEKVEEPEQQNKSSAVGPPEKVRRFTLDRLKQLGVDVSIKPRLGADEDSFVILEPETNRELEALKQRFWKHANPAAKPRAGQTVNVNVIVKDMGTDGKEELKADVVPVTLAPKKLDGASHTKPGEKLQVLKAKLQEAMKLRRFEERQKRQALFKLDNEDGFEEEEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEEEEEGNQETAEFLLSSEEIETKDEKEMDKENNDGSSEIGKAVGFLSVPKSLSSDSTLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCSLLTKESSHNSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLVSSASKSSGKLSEPSLPIEDSQDLYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVRNHRNQYQALKPRLPLASMDENAMDANMDELLDLCTGKFTSQAEKHLPRKSDKKENMEELLNLCSGKFTSQDASTPASSELNKQEKESSMGDPMEEALALCSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSDSGNDLALEDHEDDDEEELLKRSEKLKRQMRLRKYLEDEAEVSGSDVGSEDEYDGEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQLRLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESEARWRKERIEREQWLRDMAQQGKITAEEEEEIGEDSQFMILAKKVTAKALQKNASRPMVIQESKSLLRNPFEAIRPGSAQQVKTGSLLNQPKAVLQKLAALSDHNPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKHLKTDDSTSGLTRSIFKYLES
uniprot
proteins
function
FUNCTION: Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation (PubMed:12766152, PubMed:15190204, PubMed:15707391, PubMed:16123041). Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins (PubMed:12766152, PubMed:15096610, PubMed:15707391, PubMed:16123041). Also required to maintain normal rates of replication fork progression during unperturbed DNA replication. Binds directly to DNA, with particular affinity for branched or forked molecules and interacts with multiple protein components of the replisome such as the MCM2-7 complex and TIMELESS (PubMed:15226314, PubMed:34694004, PubMed:35585232). Important for initiation of DNA replication, recruits kinase CDC7 to phosphorylate MCM2-7 components (PubMed:27401717). {ECO:0000269|PubMed:12766152, ECO:0000269|PubMed:15096610, ECO:0000269|PubMed:15190204, ECO:0000269|PubMed:15226314, ECO:0000269|PubMed:15707391, ECO:0000269|PubMed:16123041, ECO:0000269|PubMed:27401717, ECO:0000269|PubMed:34694004, ECO:0000269|PubMed:35585232}.
uniprot
proteins
mass_dalton
151094
uniprot
proteins
mutagenesis
MUTAGEN 311..318; /note="NKTIHDFF->AKTAHDAA: Strongly decreases interaction with PCNA."; /evidence="ECO:0000269|PubMed:27401717"; MUTAGEN 841; /note="D->A: Does not affect cleavage by caspase-7 (CASP7)."; /evidence="ECO:0000269|PubMed:16123041"; MUTAGEN 916; /note="T->A: Impairs interaction with CHEK1."; /evidence="ECO:0000269|PubMed:15707391"; MUTAGEN 945; /note="S->A: Impairs interaction with CHEK1."; /evidence="ECO:0000269|PubMed:15707391"; MUTAGEN 982; /note="S->A: No effect on interaction with CHEK1."; /evidence="ECO:0000269|PubMed:15707391"; MUTAGEN 1031; /note="D->A: Does not affect cleavage by caspase-7 (CASP7)."; /evidence="ECO:0000269|PubMed:16123041"; MUTAGEN 1072; /note="D->A: Abolished cleavage by caspase-7 (CASP7)."; /evidence="ECO:0000269|PubMed:16123041"; MUTAGEN 1085; /note="D->A: Does not affect cleavage by caspase-7 (CASP7)."; /evidence="ECO:0000269|PubMed:16123041"; MUTAGEN 1158; /note="D->A: Does not affect cleavage by caspase-7 (CASP7)."; /evidence="ECO:0000269|PubMed:16123041
uniprot
proteins
post_translational_modification
PTM: Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding (PubMed:12766152, PubMed:15096610, PubMed:16963448, PubMed:21680713). Phosphorylated by CDC7 during DNA replication, phosphorylation inhibits interaction between the acidic patch and N-terminal segments leading to increased binding to DNA and PCNA (PubMed:27401717). {ECO:0000269|PubMed:12766152, ECO:0000269|PubMed:15096610, ECO:0000269|PubMed:16963448, ECO:0000269|PubMed:21680713, ECO:0000269|PubMed:27401717}.; PTM: Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation. {ECO:0000269|PubMed:16901786, ECO:0000269|PubMed:18662541}.; PTM: Proteolytically cleaved by caspase-7 (CASP7) in response to apoptosis, leading to its inactivation. {ECO:0000269|PubMed:16123041}.
uniprot
proteins
protein_family
Claspin family
uniprot
proteins
pubmed
11090622
uniprot
proteins
pubmed
12766152
uniprot
proteins
pubmed
15096610
uniprot
proteins
pubmed
15190204
uniprot
proteins
pubmed
15226314
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15707391
uniprot
proteins
pubmed
16123041
uniprot
proteins
pubmed
16710414
uniprot
proteins
pubmed
16901786
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
16963448
uniprot
proteins
pubmed
17081983
uniprot
proteins
pubmed
17141802
uniprot
proteins
pubmed
17525332
uniprot
proteins
pubmed
18662541
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19608861
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
21680713
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
27401717
uniprot
proteins
pubmed
34694004
uniprot
proteins
pubmed
34700328
uniprot
proteins
pubmed
35585232
uniprot
proteins
sequence_length
1339
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12766152}.
uniprot
proteins