Hint1
entrez
15254
canonical
uniprot
P70349
canonical
ensembl
ENSMUSG00000020267
resolver
ensp
ENSMUSP00000020504
resolver
enst
ENSMUST00000020504
resolver
genesymbol
Hint1
resolver
genesymbol_syn
Hint
resolver
genesymbol_syn
Pkci
resolver
genesymbol_syn
Pkci1
resolver
genesymbol_syn
Prkcnh1
resolver
uniprot_entry
HINT1_MOUSE
resolver
activity_regulation
ACTIVITY REGULATION: Desumoylase activity is inhibited by zinc ions, and inhibition is released by nitric oxide or calcium-activated calmodulin. {ECO:0000269|PubMed:31088288}.
uniprot
proteins
amino_acid_sequence
MADEIAKAQVAQPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVADDDDESLLGHLMIVGKKCAADLGLKRGYRMVVNEGADGGQSVYHIHLHVLGGRQMNWPPG
uniprot
proteins
ec_number
3.4.22.-
uniprot
proteins
ec_number
3.9.1.-
uniprot
proteins
function
FUNCTION: Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2 (By similarity). Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate) (By similarity). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester (By similarity). Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide (By similarity). In addition, functions as a scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1 (By similarity). Modulates p53/TP53 levels and p53/TP53-mediated apoptosis (PubMed:35605301). Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (By similarity). Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RANGAP1 and RGS17 (PubMed:31088288). {ECO:0000250|UniProtKB:P49773, ECO:0000269|PubMed:31088288, ECO:0000269|PubMed:35605301}.
uniprot
proteins
mass_dalton
13777
uniprot
proteins
mutagenesis
MUTAGEN 17; /note="T->A: Loss of interaction with CALM1. No effect on desumoylase activity."; /evidence="ECO:0000269|PubMed:31088288"; MUTAGEN 38; /note="C->S: No effect on desumoylase activity."; /evidence="ECO:0000269|PubMed:31088288"; MUTAGEN 84; /note="C->S: Loss of desumoylase activity."; /evidence="ECO:0000269|PubMed:31088288"; MUTAGEN 87; /note="D->V: Loss of desumoylase activity."; /evidence="ECO:0000269|PubMed:31088288"; MUTAGEN 112; /note="H->N: Reduced interaction with SUMO2."; /evidence="ECO:0000269|PubMed:31088288"; MUTAGEN 114; /note="H->R: Reduced interaction with SUMO2."; /evidence="ECO:0000269|PubMed:31088288"; MUTAGEN 115; /note="V->D: Significantly reduced interaction with SUMO2. Loss of desumoylase activity."; /evidence="ECO:0000269|PubMed:31088288"; MUTAGEN 116; /note="L->Q: Significantly reduced interaction with SUMO2."; /evidence="ECO:0000269|PubMed:31088288
uniprot
proteins
post_translational_modification
PTM: Deubiquitinated by USP40, leading to stabilization. {ECO:0000269|PubMed:35605301}.
uniprot
proteins
protein_family
HINT family
uniprot
proteins
pubmed
12810953
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
23806337
uniprot
proteins
pubmed
31088288
uniprot
proteins
pubmed
35605301
uniprot
proteins
pubmed
9770345
uniprot
proteins
sequence_length
126
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:35605301}. Nucleus {ECO:0000250|UniProtKB:P49773}.
uniprot
proteins