Rb1
entrez
19645
canonical
uniprot
P13405
canonical
ensembl
ENSMUSG00000022105
resolver
ensp
ENSMUSP00000022701
resolver
enst
ENSMUST00000022701
resolver
genesymbol
Rb1
resolver
genesymbol_syn
Rb-1
resolver
uniprot
Q4VA62
resolver
uniprot_entry
RB_MOUSE
resolver
uniprot_sec
Q4VA62
resolver
amino_acid_sequence
MPPKAPRRAAAAEPPPPPPPPPREDDPAQDSGPEELPLARLEFEEIEEPEFIALCQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDNAMSRLLKKYNVLCALYSKLERTCELIYLTQPSSALSTEINSMLVLKISWITFLLAKGEVLQMEDDLVISFQLMLCVVDYFIKFSPPALLREPYKTAAIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFINSLGIVSSNGLPEVESLSKRYEEVYLKNKDLDARLFLDHDKTLQTDPIDSFETERTPRKNNPDEEANVVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFNNCTVNPKENILKRVKDVGHIFKEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTLQHLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDNLEPACPLSLPLQGNHTAADMYLSPLRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLALFYKKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGADEADGSKHLPAESKFQQKLAEMTSTRTRMQKQRMNESKDVSNKEEK
uniprot
proteins
function
FUNCTION: Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:8336704). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes. Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription. Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase. RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation (PubMed:15750587). Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation (PubMed:16612004). Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity) (PubMed:15750587, PubMed:16612004, PubMed:8336704). {ECO:0000250|UniProtKB:P06400, ECO:0000250|UniProtKB:P33568, ECO:0000269|PubMed:15750587, ECO:0000269|PubMed:16612004, ECO:0000269|PubMed:8336704}.
uniprot
proteins
mass_dalton
105367
uniprot
proteins
mutagenesis
MUTAGEN 733..769; /note="Missing: Loss of exclusive nuclear localization. Complete loss of nuclear localization, loss of growth inhibition, when transfected in Saos-2 cells and loss of interaction with SV40 large T antigen, adenovirus E1a and E2F1; when associated with 866-N--Q-869."; /evidence="ECO:0000269|PubMed:8336704"; MUTAGEN 746; /note="I->A: Abolishes the interaction with many chromatin regulators but not that with KMT5B and KMT5C; when associated with A-750 and A-754."; /evidence="ECO:0000269|PubMed:16612004"; MUTAGEN 750; /note="N->A: Abolishes the interaction with many chromatin regulators but not that with KMT5B and KMT5C; when associated with A-746 and A-754."; /evidence="ECO:0000269|PubMed:16612004"; MUTAGEN 754; /note="M->A: Abolishes the interaction with many chromatin regulators but not that with KMT5B and KMT5C; when associated with A-746 and A-750."; /evidence="ECO:0000269|PubMed:16612004"; MUTAGEN 769..872; /note="Missing: Loss of exclusive nuclear localization and loss of growth inhibition, when transfected in Saos-2 cells. No effect on the interaction with SV40 large T antigen."; /evidence="ECO:0000269|PubMed:8336704"; MUTAGEN 853..869; /note="Missing: Loss of exclusive nuclear localization. Decreased growth inhibition activity, when transfected in Saos-2 cells. No effect on the interaction with SV40 large T antigen, adenovirus E1a, nor E2F1."; /evidence="ECO:0000269|PubMed:8336704"; MUTAGEN 866..869; /note="KKLR->NKLQ: Loss of exclusive nuclear localization, no effect on the interaction with SV40 large T antigen, adenovirus E1a, nor E2F1. Decreased growth inhibition activity, when transfected in Saos-2 cells. Complete loss of nuclear localization, loss of growth inhibition, when transfected in Saos-2 cells and loss of interaction with SV40 large T antigen, adenovirus E1a and E2F1; when associated with 733-K--P-769."; /evidence="ECO:0000269|PubMed:8336704
uniprot
proteins
post_translational_modification
PTM: Phosphorylated (PubMed:8336704). Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-561 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. Phosphorylation of threonine residues in domain C promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-788 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-800 and Ser-804 is required for G0-G1 transition (By similarity). Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity). {ECO:0000250, ECO:0000269|PubMed:8336704}.; PTM: Monomethylation at Lys-803 by SMYD2 enhances phosphorylation at Ser-800 and Ser-804, and promotes cell cycle progression. Monomethylation at Lys-853 by SMYD2 promotes interaction with L3MBTL1 (By similarity). N-terminus is methylated by METTL11A/NTM1. {ECO:0000250, ECO:0000269|PubMed:20668449}.; PTM: Acetylated in the skin (PubMed:20940255). Acetylation at Lys-866 and Lys-867 regulates subcellular localization during keratinocytes differentiation (By similarity). {ECO:0000250|UniProtKB:P06400, ECO:0000269|PubMed:20940255}.
uniprot
proteins
protein_family
Retinoblastoma protein (RB) family
uniprot
proteins
pubmed
10869426
uniprot
proteins
pubmed
10888886
uniprot
proteins
pubmed
11571651
uniprot
proteins
pubmed
12095676
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15750587
uniprot
proteins
pubmed
15983387
uniprot
proteins
pubmed
16612004
uniprot
proteins
pubmed
16616919
uniprot
proteins
pubmed
20668449
uniprot
proteins
pubmed
20940255
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
2671991
uniprot
proteins
pubmed
36689330
uniprot
proteins
pubmed
8336704
uniprot
proteins
sequence_length
921
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:36689330, ECO:0000269|PubMed:8336704}. Cytoplasm {ECO:0000269|PubMed:36689330}. Note=During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization (By similarity). Localizes to the cytoplasm when hyperphosphorylated (PubMed:36689330). {ECO:0000250|UniProtKB:P06400, ECO:0000269|PubMed:36689330}.
uniprot
proteins