RBX1
entrez
9978
canonical
uniprot
P62877
canonical
ensembl
ENSG00000100387
resolver
ensp
ENSP00000216225
resolver
enst
ENST00000216225
resolver
genesymbol
RBX1
resolver
genesymbol_syn
RNF75
resolver
genesymbol_syn
ROC1
resolver
hgnc
HGNC:9928
resolver
uniprot
B2RDY1
resolver
uniprot
Q8N6Z8
resolver
uniprot
Q9D1S2
resolver
uniprot
Q9WUK9
resolver
uniprot
Q9Y254
resolver
uniprot_entry
RBX1_HUMAN
resolver
uniprot_sec
B2RDY1
resolver
uniprot_sec
Q8N6Z8
resolver
uniprot_sec
Q9D1S2
resolver
uniprot_sec
Q9WUK9
resolver
uniprot_sec
Q9Y254
resolver
amino_acid_sequence
MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
ec_number
2.3.2.32
uniprot
proteins
function
FUNCTION: E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair (PubMed:10230407, PubMed:10579999, PubMed:11961546, PubMed:15983046, PubMed:16678110, PubMed:19112177, PubMed:19679664, PubMed:22748924, PubMed:23455478, PubMed:27565346, PubMed:29769719, PubMed:32355176, PubMed:33417871, PubMed:37844242, PubMed:38326650, PubMed:39504960, PubMed:39667934, PubMed:38316879). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (PubMed:27565346). The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex mediates ubiquitination of Pol II subunit POLR2A at 'Lys-1268', a critical TC-NER checkpoint (PubMed:32355176, PubMed:34526721). Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:35982156). Core component of a Cul9-RING ubiquitin ligase complex composed of CUL9 and RBX1, which mediates mono-ubiquitination of p53/TP53 (PubMed:38605244). Recruits the E2 ubiquitin-conjugating enzyme CDC34 to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. As part of a multisubunit complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5; polyubiquitinates monoubiquitinated POLR2A (PubMed:19920177). {ECO:0000269|PubMed:10230407, ECO:0000269|PubMed:10579999, ECO:0000269|PubMed:11027288, ECO:0000269|PubMed:11961546, ECO:0000269|PubMed:15983046, ECO:0000269|PubMed:16678110, ECO:0000269|PubMed:16751180, ECO:0000269|PubMed:18397884, ECO:0000269|PubMed:19112177, ECO:0000269|PubMed:19679664, ECO:0000269|PubMed:19920177, ECO:0000269|PubMed:22748924, ECO:0000269|PubMed:23455478, ECO:0000269|PubMed:27565346, ECO:0000269|PubMed:29769719, ECO:0000269|PubMed:32355176, ECO:0000269|PubMed:33417871, ECO:0000269|PubMed:34526721, ECO:0000269|PubMed:35982156, ECO:0000269|PubMed:37844242, ECO:0000269|PubMed:38316879, ECO:0000269|PubMed:38326650, ECO:0000269|PubMed:38605244, ECO:0000269|PubMed:39504960, ECO:0000269|PubMed:39667934}.
uniprot
proteins
mass_dalton
12274
uniprot
proteins
mutagenesis
MUTAGEN 53; /note="C->A: Strong reduction in ligase activity; when associated with A-56."; /evidence="ECO:0000269|PubMed:10230407"; MUTAGEN 56; /note="C->A: Strong reduction in ligase activity; when associated with A-53."; /evidence="ECO:0000269|PubMed:10230407"; MUTAGEN 75; /note="C->A: Strong reduction in ligase activity; when associated with A-77."; /evidence="ECO:0000269|PubMed:10230407"; MUTAGEN 77; /note="H->A: Strong reduction in ligase activity; when associated with A-75."; /evidence="ECO:0000269|PubMed:10230407"; MUTAGEN 91; /note="R->E: Impairs UBE2R2 activation by neddylated Cul2-RING(FEM1C) complex."; /evidence="ECO:0000269|PubMed:38326650
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:10230407, ECO:0000269|PubMed:11027288, ECO:0000269|PubMed:22748924, ECO:0000269|PubMed:37844242, ECO:0000269|PubMed:38326650, ECO:0000269|PubMed:38605244, ECO:0000269|PubMed:39504960, ECO:0000269|PubMed:39667934}.; PATHWAY: Protein modification; protein neddylation. {ECO:0000269|PubMed:11027288}.
uniprot
proteins
protein_family
RING-box family
uniprot
proteins
pubmed
10213691
uniprot
proteins
pubmed
10230406
uniprot
proteins
pubmed
10230407
uniprot
proteins
pubmed
10579999
uniprot
proteins
pubmed
10591208
uniprot
proteins
pubmed
10643962
uniprot
proteins
pubmed
11027288
uniprot
proteins
pubmed
11337588
uniprot
proteins
pubmed
11384984
uniprot
proteins
pubmed
11961546
uniprot
proteins
pubmed
12149480
uniprot
proteins
pubmed
12481031
uniprot
proteins
pubmed
12732143
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
14739464
uniprot
proteins
pubmed
15461802
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15983046
uniprot
proteins
pubmed
16678110
uniprot
proteins
pubmed
16751180
uniprot
proteins
pubmed
18397884
uniprot
proteins
pubmed
18691976
uniprot
proteins
pubmed
18809579
uniprot
proteins
pubmed
19112177
uniprot
proteins
pubmed
19250909
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19679664
uniprot
proteins
pubmed
19920177
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
20596027
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22223895
uniprot
proteins
pubmed
22748924
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
23274085
uniprot
proteins
pubmed
23455478
uniprot
proteins
pubmed
24192928
uniprot
proteins
pubmed
25349211
uniprot
proteins
pubmed
25684205
uniprot
proteins
pubmed
26906416
uniprot
proteins
pubmed
27565346
uniprot
proteins
pubmed
29149593
uniprot
proteins
pubmed
29769719
uniprot
proteins
pubmed
32355176
uniprot
proteins
pubmed
33417871
uniprot
proteins
pubmed
34526721
uniprot
proteins
pubmed
34700328
uniprot
proteins
pubmed
35687106
uniprot
proteins
pubmed
35982156
uniprot
proteins
pubmed
37844242
uniprot
proteins
pubmed
38225631
uniprot
proteins
pubmed
38316879
uniprot
proteins
pubmed
38326650
uniprot
proteins
pubmed
38605244
uniprot
proteins
pubmed
39504960
uniprot
proteins
pubmed
39667934
uniprot
proteins
pubmed
39880951
uniprot
proteins
sequence_length
108
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11027288}. Nucleus {ECO:0000269|PubMed:11027288}.
uniprot
proteins