NEK2
entrez
4751
canonical
uniprot
P51955
canonical
ensembl
ENSG00000117650
resolver
ensp
ENSP00000355965
resolver
ensp
ENSP00000355966
resolver
ensp
ENSP00000440237
resolver
enst
ENST00000540251
resolver
genesymbol
NEK2
resolver
genesymbol_syn
NEK2A
resolver
genesymbol_syn
NLK1
resolver
hgnc
HGNC:7745
resolver
uniprot
F6U4U2
resolver
uniprot
Q53FD6
resolver
uniprot
Q5I1Z9
resolver
uniprot
Q5VXZ1
resolver
uniprot
Q6NZX8
resolver
uniprot
Q7Z634
resolver
uniprot
Q86XH2
resolver
uniprot
Q96QN9
resolver
uniprot_entry
F6U4U2_HUMAN
resolver
uniprot_entry
NEK2_HUMAN
resolver
uniprot_sec
Q53FD6
resolver
uniprot_sec
Q5I1Z9
resolver
uniprot_sec
Q5VXZ1
resolver
uniprot_sec
Q6NZX8
resolver
uniprot_sec
Q7Z634
resolver
uniprot_sec
Q86XH2
resolver
uniprot_sec
Q96QN9
resolver
activity_regulation
ACTIVITY REGULATION: Isoform 1 is inhibited by ionizing radiation in the presence of PPP1CA. Its catalytic activity is inhibited by the inhibitor CCT241950. In the presence of this inhibitor, displays an autoinhibited conformation: Tyr-70 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. {ECO:0000269|PubMed:15161910, ECO:0000269|PubMed:17283141, ECO:0000269|PubMed:19941817}.
uniprot
proteins
amino_acid_sequence
MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR
uniprot
proteins
disease_involvement
DISEASE: Retinitis pigmentosa 67 (RP67) [MIM:615565]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. {ECO:0000269|PubMed:24043777}. Note=The disease is caused by variants affecting the gene represented in this entry.
uniprot
proteins
ec_number
2.7.11.1
uniprot
proteins
function
FUNCTION: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856). Phosphorylates CCDC102B either directly or indirectly which causes CCDC102B to dissociate from the centrosome and allows for centrosome separation (PubMed:30404835). {ECO:0000269|PubMed:11742531, ECO:0000269|PubMed:12857871, ECO:0000269|PubMed:14978040, ECO:0000269|PubMed:15358203, ECO:0000269|PubMed:15388344, ECO:0000269|PubMed:17283141, ECO:0000269|PubMed:17621308, ECO:0000269|PubMed:17626005, ECO:0000269|PubMed:18086858, ECO:0000269|PubMed:18297113, ECO:0000269|PubMed:20034488, ECO:0000269|PubMed:21076410, ECO:0000269|PubMed:24554434, ECO:0000269|PubMed:25704143, ECO:0000269|PubMed:26220856, ECO:0000269|PubMed:30404835}.; FUNCTION: [Isoform 1]: Phosphorylates and activates NEK11 in G1/S-arrested cells. {ECO:0000269|PubMed:15161910}.; FUNCTION: [Isoform 2]: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells. {ECO:0000269|PubMed:15161910}.
uniprot
proteins
mass_dalton
51763
uniprot
proteins
mutagenesis
MUTAGEN 37; /note="K->R: Loss of kinase activity and of ability to activate NEK11. Loss of phosphorylation of CCDC102B."; /evidence="ECO:0000269|PubMed:15161910, ECO:0000269|PubMed:17197699, ECO:0000269|PubMed:30404835"; MUTAGEN 141; /note="D->A: Loss of autophosphorylation."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 170; /note="T->A: No effect on kinase activity."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 170; /note="T->E: Kinase activity increased by two fold."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 171; /note="S->A: No effect on kinase activity."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 171; /note="S->D: Kinase activity increased by two fold."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 175; /note="T->A: Kinase activity decreased by two fold."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 175; /note="T->E: Kinase activity increased by two fold."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 179; /note="T->A: Loss of kinase activity."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 179; /note="T->E: Loss of kinase activity."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 241; /note="S->A: Loss of kinase activity."; /evidence="ECO:0000269|PubMed:17197699"; MUTAGEN 241; /note="S->D: Loss of kinase activity."; /evidence="ECO:0000269|PubMed:17197699
uniprot
proteins
post_translational_modification
PTM: Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome. {ECO:0000269|PubMed:17197699, ECO:0000269|PubMed:21076410}.
uniprot
proteins
protein_family
NEK Ser/Thr protein kinase family
uniprot
proteins
protein_family
NIMA subfamily
uniprot
proteins
protein_family
Protein kinase superfamily
uniprot
proteins
pubmed
11742531
uniprot
proteins
pubmed
12857871
uniprot
proteins
pubmed
14654843
uniprot
proteins
pubmed
14978040
uniprot
proteins
pubmed
15161910
uniprot
proteins
pubmed
15358203
uniprot
proteins
pubmed
15388344
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15659832
uniprot
proteins
pubmed
16280549
uniprot
proteins
pubmed
16710414
uniprot
proteins
pubmed
17197699
uniprot
proteins
pubmed
17283141
uniprot
proteins
pubmed
17344846
uniprot
proteins
pubmed
17621308
uniprot
proteins
pubmed
17626005
uniprot
proteins
pubmed
18086858
uniprot
proteins
pubmed
18088087
uniprot
proteins
pubmed
18297113
uniprot
proteins
pubmed
18691976
uniprot
proteins
pubmed
19369195
uniprot
proteins
pubmed
19941817
uniprot
proteins
pubmed
20034488
uniprot
proteins
pubmed
20599736
uniprot
proteins
pubmed
21076410
uniprot
proteins
pubmed
21669869
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24043777
uniprot
proteins
pubmed
24554434
uniprot
proteins
pubmed
25704143
uniprot
proteins
pubmed
26220856
uniprot
proteins
pubmed
26471995
uniprot
proteins
pubmed
30404835
uniprot
proteins
pubmed
7522034
uniprot
proteins
pubmed
8274451
uniprot
proteins
sequence_length
445
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: [Isoform 1]: Nucleus. Nucleus, nucleolus {ECO:0000269|PubMed:15161910}. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:14654843, ECO:0000269|PubMed:26220856}. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere {ECO:0000250}. Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGO1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm. Note=Predominantly cytoplasmic.; SUBCELLULAR LOCATION: [Isoform 4]: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear.
uniprot
proteins