Nsfl1c
entrez
83809
canonical
uniprot
O35987
canonical
ensembl
ENSRNOG00000008604
resolver
ensp
ENSRNOP00000074814
resolver
genesymbol
Nsfl1c
resolver
uniprot_entry
NSF1C_RAT
resolver
amino_acid_sequence
MAEERQDALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEEEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGERRRHSGQDVHVVLKLWKTGFSLDNGDLRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLNTSSPAQQAENEAKASSSILINEAEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFVLMTTFPNKELADENQTLKEANLLNAVIVQRLT
uniprot
proteins
function
FUNCTION: Reduces the ATPase activity of VCP (PubMed:9214505, PubMed:9824302). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (PubMed:12411482). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (PubMed:10930451). Inhibits the activity of CTSL (in vitro) (By similarity). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (By similarity). Also, regulates spindle orientation during mitosis (By similarity). {ECO:0000250|UniProtKB:Q9UNZ2, ECO:0000269|PubMed:10930451, ECO:0000269|PubMed:12411482, ECO:0000269|PubMed:9214505, ECO:0000269|PubMed:9824302}.
uniprot
proteins
mass_dalton
40680
uniprot
proteins
mutagenesis
MUTAGEN 41; /note="F->A: Reduces ubiquitin binding and Golgi reassembly."; /evidence="ECO:0000269|PubMed:12411482"; MUTAGEN 57; /note="T->A: No effect on phosphorylation."; /evidence="ECO:0000269|PubMed:12810701"; MUTAGEN 112; /note="K->T: Strongly reduces nuclear location."; /evidence="ECO:0000269|PubMed:12810701"; MUTAGEN 114; /note="S->A: No effect on phosphorylation."; /evidence="ECO:0000269|PubMed:12810701"; MUTAGEN 140; /note="S->A: Abolishes phosphorylation by CDK1."; /evidence="ECO:0000269|PubMed:12810701"; MUTAGEN 140; /note="S->D: Strongly reduces binding to Golgi membranes."; /evidence="ECO:0000269|PubMed:12810701"; MUTAGEN 173; /note="R->T: Strongly reduces nuclear location."; /evidence="ECO:0000269|PubMed:12810701"; MUTAGEN 272; /note="S->A: No effect on phosphorylation."; /evidence="ECO:0000269|PubMed:12810701"; MUTAGEN 301; /note="R->A: Reduced interaction with VCP."; /evidence="ECO:0000269|PubMed:14988733"; MUTAGEN 342..345; /note="TFPN->AG: Strongly reduced interaction with VCP."; /evidence="ECO:0000269|PubMed:14988733"; MUTAGEN 343; /note="F->S: Reduced interaction with VCP."; /evidence="ECO:0000269|PubMed:14988733"; MUTAGEN 345; /note="N->A: Reduced interaction with VCP."; /evidence="ECO:0000269|PubMed:14988733
uniprot
proteins
post_translational_modification
PTM: Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis. {ECO:0000269|PubMed:12810701}.
uniprot
proteins
protein_family
NSFL1C family
uniprot
proteins
pubmed
10811609
uniprot
proteins
pubmed
10930451
uniprot
proteins
pubmed
11478859
uniprot
proteins
pubmed
12146947
uniprot
proteins
pubmed
12411482
uniprot
proteins
pubmed
12473691
uniprot
proteins
pubmed
12810701
uniprot
proteins
pubmed
1495983
uniprot
proteins
pubmed
14988733
uniprot
proteins
pubmed
15029246
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16641100
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
23649807
uniprot
proteins
pubmed
9214505
uniprot
proteins
pubmed
9297509
uniprot
proteins
pubmed
9506515
uniprot
proteins
pubmed
9824302
uniprot
proteins
sequence_length
370
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12810701}. Golgi apparatus, Golgi stack {ECO:0000269|PubMed:12810701, ECO:0000269|PubMed:9214505}. Chromosome {ECO:0000269|PubMed:1495983}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:23649807}. Note=Predominantly nuclear in interphase cells (PubMed:12810701). Bound to the axial elements of sex chromosomes in pachytene spermatocytes (PubMed:1495983). A small proportion of the protein is cytoplasmic, associated with Golgi stacks (PubMed:12810701). Localizes to centrosome during mitotic prophase and metaphase (PubMed:23649807). {ECO:0000269|PubMed:12810701, ECO:0000269|PubMed:1495983, ECO:0000269|PubMed:23649807}.
uniprot
proteins