Axin1
entrez
12005
canonical
uniprot
O35625
canonical
ensembl
ENSMUSG00000024182
resolver
ensp
ENSMUSP00000073974
resolver
ensp
ENSMUSP00000305591
resolver
ensp
ENSMUSP00000305592
resolver
ensp
ENSMUSP00001091662
resolver
enst
ENSMUST00000074370
resolver
genesymbol
Axin1
resolver
genesymbol_syn
Axin
resolver
genesymbol_syn
Fu
resolver
uniprot
E9QMJ8
resolver
uniprot
Q14DJ8
resolver
uniprot_entry
AXIN1_MOUSE
resolver
uniprot_entry
E9QMJ8_MOUSE
resolver
uniprot_entry
Q14DJ8_MOUSE
resolver
amino_acid_sequence
MNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRYVQAVMQRGRTCVRPACAPVLSVVPAVSDLELSETETKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD
uniprot
proteins
function
FUNCTION: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed:12223491, PubMed:17681137). In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (By similarity). Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity). Also a component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed:15526030). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed:15526030). Activates the AMP-activated protein kinase (AMPK) in response to calorie restriction by acting as a molecular adapter: recruited to late endosome membrane by the V-ATPase complex downstream of TULP3, and mediates association between STK11 and its AMPK substrates PRKAA1 and/or PRKAA2, leading to AMPK activation (PubMed:24093678, PubMed:25002183). {ECO:0000250|UniProtKB:O15169, ECO:0000269|PubMed:12223491, ECO:0000269|PubMed:15526030, ECO:0000269|PubMed:17681137, ECO:0000269|PubMed:24093678, ECO:0000269|PubMed:25002183}.
uniprot
proteins
mass_dalton
96276
uniprot
proteins
mutagenesis
MUTAGEN 480; /note="T->A: Greatly reduced GSK3B-mediated phosphorylation; when associated with A-485 and A-492."; /evidence="ECO:0000269|PubMed:10581160"; MUTAGEN 485; /note="S->A: Greatly reduced GSK3B-mediated phosphorylation and large effect on the inhibitory activity in Wnt-signaling; when associated with A-480 and A-492."; /evidence="ECO:0000269|PubMed:10581160"; MUTAGEN 492; /note="S->A: Greatly reduced GSK3B-mediated phosphorylation; when associated with A-480 and A-485."; /evidence="ECO:0000269|PubMed:10581160"; MUTAGEN 492; /note="S->I: Little effect on inhibitory activity on Wnt-signaling."; /evidence="ECO:0000269|PubMed:10581160"; MUTAGEN 495; /note="S->A: No effect on phosphorylation. Little effect on inhibitory activity on Wnt-signaling."; /evidence="ECO:0000269|PubMed:10581160"; MUTAGEN 858..863; /note="Missing: Abolishes binding of PIAS1 and PIAS2. Dramatically reduces sumoylation and abolishes AXIN1-mediated JNK activation. No effect on homodimerization nor on Wnt-signaling."; /evidence="ECO:0000269|PubMed:12223491"; MUTAGEN 858; /note="K->A: Decreased sumoylation followed by increased ubiquitination; when associated with A-861."; /evidence="ECO:0000269|PubMed:18632848"; MUTAGEN 861; /note="K->A: Decreased sumoylation followed by increased ubiquitination; when associated with A-858."; /evidence="ECO:0000269|PubMed:18632848
uniprot
proteins
post_translational_modification
PTM: Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1 (By similarity). Also phosphorylated by CDK2 which regulates interaction with CTNBB1. {ECO:0000250, ECO:0000269|PubMed:10581160, ECO:0000269|PubMed:15063782}.; PTM: ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway (By similarity). {ECO:0000250}.; PTM: Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription (By similarity). Sumoylation at Lys-858 and Lys-861 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Ubiquitination by SIAH1 and SIAH2 induces its proteasomal degradation as part of the activation of the Wnt signaling pathway (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:O15169, ECO:0000269|PubMed:12223491, ECO:0000269|PubMed:18632848}.
uniprot
proteins
pubmed
10581160
uniprot
proteins
pubmed
11336703
uniprot
proteins
pubmed
12183362
uniprot
proteins
pubmed
12223491
uniprot
proteins
pubmed
15063782
uniprot
proteins
pubmed
15262978
uniprot
proteins
pubmed
15526030
uniprot
proteins
pubmed
16815997
uniprot
proteins
pubmed
17681137
uniprot
proteins
pubmed
18632848
uniprot
proteins
pubmed
19141611
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
19581931
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
24093678
uniprot
proteins
pubmed
25002183
uniprot
proteins
pubmed
9230313
uniprot
proteins
sequence_length
863
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16815997, ECO:0000269|PubMed:19141611}. Nucleus {ECO:0000250|UniProtKB:O15169}. Cell membrane {ECO:0000269|PubMed:16815997}. Membrane {ECO:0000269|PubMed:19141611}. Late endosome {ECO:0000269|PubMed:25002183}. Note=On UV irradiation, translocates to the nucleus and colocalizes with DAAX (By similarity). MACF1 is required for its translocation to cell membrane (PubMed:16815997). {ECO:0000250|UniProtKB:O15169, ECO:0000269|PubMed:16815997}.
uniprot
proteins