Rfwd3
entrez
234736
canonical
uniprot
Q8CIK8
canonical
ensembl
ENSMUSG00000033596
resolver
ensp
ENSMUSP00000043780
resolver
enst
ENSMUST00000038739
resolver
genesymbol
Rfwd3
resolver
genesymbol_syn
Rnf201
resolver
uniprot
Q3UV90
resolver
uniprot
Q6PJA8
resolver
uniprot
Q8R2T9
resolver
uniprot
Q8R351
resolver
uniprot_entry
RFWD3_MOUSE
resolver
uniprot_sec
Q3UV90
resolver
uniprot_sec
Q6PJA8
resolver
uniprot_sec
Q8R2T9
resolver
uniprot_sec
Q8R351
resolver
amino_acid_sequence
MAEVAVGQASDLVPSEMDHEVIYSHLQGPLEGTIEPATPTEVVSNGAPLQPAPAELANSQGGAHVQPAPAEVVSSQDGLPTLQPLPPASIDLTEEVQPSEENMEVVNPGTSEEPSQGSGANPTAGAARSVSMNNFISGLQRLHNMLELLRPPPADHSVGPIRTRRRMAPILRARAGESQRQDNGRYVPHTPLYAYFQVSRSQPYPLPAAHDSETRNPPSGTDSDSDGSAEDEEVVVQAEEPEANIPEQGVIATDQEATSVTGDDAVPKESPQKPNMLSAMEDEEGETCTICLEQWTNAGDHRISALRCGHLFGFRCISKWLKGQTRKCPQCNKKAKHSDIVVIYARSLRALDTSEQERMKSDLLNEQMLRKQAELESAQCRLQLQVLIDKCTKLNSRVQDLEKFVVRHQNSAAQQCSRSKALCGKCLSSSQSPGKYSSEKTFTISQTGKCRILAYCDGLSCLVASQPSPQASFLPGFGVKMLSTANMKSSQYIPMHNKQIRGLSFSSQSKGLLLSASLDCTIKLTSLETNTVVQTYNTGRPVWSCCWCLDENNYVYAGLASGSILIYDLRNTNTYIQELVPQKARCPLVSLSYLPKAAVAAFPYGGVLAGTLENASFWELKSDFSHKPHVLSLEPGGFVDFQTESSTRHCLVTYRPDKSHNTVRTVLLEMSYKLDDAGEPVCSCLPVQTFLGGPTCKLLTKSAIFQSPENNGNVLVCTGDEASQSTLLWNAGSGLLLQNLKAGEPVLDICPFETNQNSYLATLTEKTVHMYKWE
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
function
FUNCTION: E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Required to translesion DNA synthesis across DNA-protein cross-link adducts by catalyzing ubiquitination of proteins on single-stranded DNA (ssDNA). {ECO:0000250|UniProtKB:Q6PCD5}.
uniprot
proteins
mass_dalton
84312
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination. {ECO:0000250|UniProtKB:Q6PCD5}.
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Ser-59 and Ser-75 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. Part of the phosphorylation depends upon RPA2 presence. {ECO:0000250|UniProtKB:Q6PCD5}.
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
28691929
uniprot
proteins
sequence_length
774
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q6PCD5}. Nucleus, PML body {ECO:0000250|UniProtKB:Q6PCD5}. Cytoplasm {ECO:0000250|UniProtKB:Q6PCD5}. Note=In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies. In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair. This recruitment depends upon RPA2. {ECO:0000250|UniProtKB:Q6PCD5}.
uniprot
proteins