GEN1
entrez
348654
canonical
uniprot
Q17RS7
canonical
ensembl
ENSG00000178295
resolver
ensp
ENSP00000318977
resolver
ensp
ENSP00000370653
resolver
ensp
ENSP00000532204
resolver
ensp
ENSP00000532205
resolver
ensp
ENSP00000532206
resolver
ensp
ENSP00000532207
resolver
ensp
ENSP00000582318
resolver
ensp
ENSP00000582319
resolver
ensp
ENSP00000582320
resolver
ensp
ENSP00000582321
resolver
enst
ENST00000317402
resolver
enst
ENST00000381254
resolver
enst
ENST00000862145
resolver
enst
ENST00000862146
resolver
enst
ENST00000862147
resolver
enst
ENST00000862148
resolver
enst
ENST00000912259
resolver
enst
ENST00000912260
resolver
enst
ENST00000912261
resolver
enst
ENST00000912262
resolver
genesymbol
GEN1
resolver
hgnc
HGNC:26881
resolver
uniprot
Q17RS9
resolver
uniprot
Q6ZN37
resolver
uniprot_entry
GEN_HUMAN
resolver
uniprot_sec
Q17RS9
resolver
uniprot_sec
Q6ZN37
resolver
amino_acid_sequence
MGVNDLWQILEPVKQHIPLRNLGGKTIAVDLSLWVCEAQTVKKMMGSVMKPHLRNLFFRISYLTQMDVKLVFVMEGEPPKLKADVISKRNQSRYGSSGKSWSQKTGRSHFKSVLRECLHMLECLGIPWVQAAGEAEAMCAYLNAGGHVDGCLTNDGDTFLYGAQTVYRNFTMNTKDPHVDCYTMSSIKSKLGLDRDALVGLAILLGCDYLPKGVPGVGKEQALKLIQILKGQSLLQRFNRWNETSCNSSPQLLVTKKLAHCSVCSHPGSPKDHERNGCRLCKSDKYCEPHDYEYCCPCEWHRTEHDRQLSEVENNIKKKACCCEGFPFHEVIQEFLLNKDKLVKVIRYQRPDLLLFQRFTLEKMEWPNHYACEKLLVLLTHYDMIERKLGSRNSNQLQPIRIVKTRIRNGVHCFEIEWEKPEHYAMEDKQHGEFALLTIEEESLFEAAYPEIVAVYQKQKLEIKGKKQKRIKPKENNLPEPDEVMSFQSHMTLKPTCEIFHKQNSKLNSGISPDPTLPQESISASLNSLLLPKNTPCLNAQEQFMSSLRPLAIQQIKAVSKSLISESSQPNTSSHNISVIADLHLSTIDWEGTSFSNSPAIQRNTFSHDLKSEVESELSAIPDGFENIPEQLSCESERYTANIKKVLDEDSDGISPEEHLLSGITDLCLQDLPLKERIFTKLSYPQDNLQPDVNLKTLSILSVKESCIANSGSDCTSHLSKDLPGIPLQNESRDSKILKGDQLLQEDYKVNTSVPYSVSNTVVKTCNVRPPNTALDHSRKVDMQTTRKILMKKSVCLDRHSSDEQSAPVFGKAKYTTQRMKHSSQKHNSSHFKESGHNKLSSPKIHIKETEQCVRSYETAENEESCFPDSTKSSLSSLQCHKKENNSGTCLDSPLPLRQRLKLRFQST
uniprot
proteins
ec_number
3.1.-.-
uniprot
proteins
function
FUNCTION: Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation (PubMed:19020614, PubMed:26682650). Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction (PubMed:26578604). Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA (PubMed:28049850). Also has endonuclease activity on 5'-flap and replication fork (RF) DNA substrates (PubMed:26578604). {ECO:0000269|PubMed:19020614, ECO:0000269|PubMed:26578604, ECO:0000269|PubMed:26682650, ECO:0000269|PubMed:28049850}.
uniprot
proteins
mass_dalton
102884
uniprot
proteins
mutagenesis
MUTAGEN 30; /note="D->N: Abolishes endonuclease activity on both Hollyday junctions and 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 36; /note="C->E: Abolishes endonuclease activity on both Hollyday junctions and 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 54; /note="R->E: Reduces by 50% endonuclease activity on both Hollyday junctions and 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 89; /note="R->E: No effect on endonuclease activity on Hollyday junctions. Slightly reduces endonuclease activity on 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 93; /note="R->E: No effect on endonuclease activity on Hollyday junctions. Slightly reduces endonuclease activity on 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 109; /note="H->E: Strongly reduces endonuclease activity on both Hollyday junctions and 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 110; /note="F->E: Reduces by 25% endonuclease activity on Hollyday junctions and by 65% on 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 134..136; /note="EAE->AAA: Abolishes endonuclease activity."; /evidence="ECO:0000269|PubMed:26578604"; MUTAGEN 380; /note="T->E: No effect on endonuclease activity on both Hollyday junctions and 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 404; /note="K->E: No effect on endonuclease activity on both Hollyday junctions and 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 406; /note="R->E: No effect on endonuclease activity on both Hollyday junctions and 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650"; MUTAGEN 438; /note="T->E: No effect on endonuclease activity on both Hollyday junctions and 5' flap substrates."; /evidence="ECO:0000269|PubMed:26682650
uniprot
proteins
protein_family
GEN subfamily
uniprot
proteins
protein_family
XPG/RAD2 endonuclease family
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15815621
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
19020614
uniprot
proteins
pubmed
21248752
uniprot
proteins
pubmed
26578604
uniprot
proteins
pubmed
26682650
uniprot
proteins
pubmed
28049850
uniprot
proteins
sequence_length
908
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:26578604, ECO:0000305|PubMed:28049850}.
uniprot
proteins