Slc12a1
entrez
20495
canonical
uniprot
P55014
canonical
ensembl
ENSMUSG00000027202
resolver
ensp
ENSMUSP00000028630
resolver
ensp
ENSMUSP00000106120
resolver
ensp
ENSMUSP00000106121
resolver
enst
ENSMUST00000028630
resolver
enst
ENSMUST00000110494
resolver
enst
ENSMUST00000110495
resolver
genesymbol
Slc12a1
resolver
genesymbol_syn
Nkcc2
resolver
uniprot
A2AQ50
resolver
uniprot
A2AQ51
resolver
uniprot
A2AQ52
resolver
uniprot
O35938
resolver
uniprot
Q91W55
resolver
uniprot
Q9Z1E0
resolver
uniprot_entry
A2AQ50_MOUSE
resolver
uniprot_entry
A2AQ51_MOUSE
resolver
uniprot_entry
A2AQ52_MOUSE
resolver
uniprot_entry
S12A1_MOUSE
resolver
uniprot_sec
O35938
resolver
uniprot_sec
Q91W55
resolver
uniprot_sec
Q9Z1E0
resolver
activity_regulation
ACTIVITY REGULATION: Activated following phosphorylation by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4). {ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:21972418}.; ACTIVITY REGULATION: [Isoform A]: Inhibited by mercury dichloride and diuretic drug bumetaide. Inactive in isotonic conditions. {ECO:0000269|PubMed:11790783}.; ACTIVITY REGULATION: [Isoform B]: Inhibited by mercury dichloride and diuretic drug bumetaide. Inactive in isotonic conditions. {ECO:0000269|PubMed:11790783}.; ACTIVITY REGULATION: [Isoform F]: Inhibited by mercury dichloride and diuretic drug bumetaide. Inactive in isotonic conditions. {ECO:0000269|PubMed:11790783}.
uniprot
proteins
amino_acid_sequence
MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS
uniprot
proteins
function
FUNCTION: Renal sodium, potassium and chloride non-electrogenic ion symporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (Probable) (PubMed:11790783). It can substitute NH4(+) for K(+), enabling NH4(+) apical transmembrane transport in the medullary thick ascending limb (MTAL). This function is crucial for maintaining ammonium homeostasis by the kidney, particularly during metabolic acidosis (By similarity). {ECO:0000250|UniProtKB:P55015, ECO:0000269|PubMed:11790783, ECO:0000305|PubMed:25967121}.; FUNCTION: [Isoform A]: High affinity, high capacity cotransporter for sodium, potassium and chloride ions, with a coupling ratio 1Na(+):1K(+):2Cl(-). {ECO:0000269|PubMed:11790783}.; FUNCTION: [Isoform B]: High affinity, low capacity cotransporter for sodium, potassium and chloride ions, with a coupling ratio 1Na(+):1K(+):2Cl(-). {ECO:0000269|PubMed:11790783}.; FUNCTION: [Isoform F]: Low affinity, low capacity cotransporter for sodium, potassium and chloride ions, with a coupling ratio 1Na(+):1K(+):2Cl(-). {ECO:0000269|PubMed:11790783}.
uniprot
proteins
mass_dalton
120355
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Ser-87, Thr-96 and Thr-101 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity (PubMed:21321328). Short-term cyclosporine administration increases SLC12A1 phosphorylation in kidney thick ascending limb, possibly through the inhibition of PPP3CB/calcineurin A beta phosphatase (PubMed:25967121). {ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:25967121}.
uniprot
proteins
protein_family
SLC12A transporter family
uniprot
proteins
pubmed
10070158
uniprot
proteins
pubmed
11790783
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
20385770
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21321328
uniprot
proteins
pubmed
21972418
uniprot
proteins
pubmed
25967121
uniprot
proteins
pubmed
29993276
uniprot
proteins
pubmed
7573490
uniprot
proteins
sequence_length
1095
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000269|PubMed:29993276}; Multi-pass membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 174..194; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 198..218; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 256..276; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 299..319; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 324..344; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 376..396; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 414..434; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 547..567; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 568..588; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 606..626; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins