Ap2m1
entrez
11773
canonical
uniprot
P84091
canonical
ensembl
ENSMUSG00000022841
resolver
ensp
ENSMUSP00000007216
resolver
ensp
ENSMUSP00000087477
resolver
enst
ENSMUST00000007216
resolver
enst
ENSMUST00000090023
resolver
genesymbol
Ap2m1
resolver
genesymbol_syn
Clapm1
resolver
genesymbol_syn
mKIAA0109
resolver
uniprot
F8WGI3
resolver
uniprot
P20172
resolver
uniprot
P53679
resolver
uniprot
Q3TWV4
resolver
uniprot
Q5FWI9
resolver
uniprot
Q6A0C9
resolver
uniprot_entry
AP2M1_MOUSE
resolver
uniprot_entry
F8WGI3_MOUSE
resolver
uniprot_entry
Q3TWV4_MOUSE
resolver
uniprot_entry
Q5FWI9_MOUSE
resolver
uniprot_entry
Q6A0C9_MOUSE
resolver
uniprot_sec
P20172
resolver
uniprot_sec
P53679
resolver
amino_acid_sequence
MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC
uniprot
proteins
function
FUNCTION: Component of the adaptor protein complex 2 (AP-2) (PubMed:14745134, PubMed:15473838). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways (PubMed:14745134, PubMed:15473838). Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation (PubMed:14745134, PubMed:15473838). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome (PubMed:14745134, PubMed:15473838). The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components (PubMed:14745134, PubMed:15473838). Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation (By similarity). AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis (By similarity). AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface (By similarity). AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules (By similarity). AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway (By similarity). During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs (By similarity). The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2 (By similarity). The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity). {ECO:0000250|UniProtKB:P84092, ECO:0000250|UniProtKB:Q96CW1, ECO:0000269|PubMed:14745134, ECO:0000269|PubMed:15473838, ECO:0000269|PubMed:23676497}.
uniprot
proteins
mass_dalton
49655
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Thr-156 increases the affinity of the AP-2 complex for cargo membrane proteins during the initial stages of endocytosis. {ECO:0000250|UniProtKB:Q96CW1}.
uniprot
proteins
protein_family
Adaptor complexes medium subunit family
uniprot
proteins
pubmed
10910768
uniprot
proteins
pubmed
14530274
uniprot
proteins
pubmed
14745134
uniprot
proteins
pubmed
15473838
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16227583
uniprot
proteins
pubmed
16707488
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
23676497
uniprot
proteins
pubmed
7569928
uniprot
proteins
pubmed
9573360
uniprot
proteins
sequence_length
435
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q96CW1}. Membrane, coated pit {ECO:0000250|UniProtKB:Q96CW1}; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. {ECO:0000305|PubMed:14530274}.
uniprot
proteins