Gpn1
entrez
74254
canonical
uniprot
Q8VCE2
canonical
ensembl
ENSMUSG00000064037
resolver
ensp
ENSMUSP00000076217
resolver
enst
ENSMUST00000076949
resolver
genesymbol
Gpn1
resolver
genesymbol_syn
Mbdin
resolver
genesymbol_syn
Xab1
resolver
uniprot
Q4VAB2
resolver
uniprot_entry
GPN1_MOUSE
resolver
uniprot_entry
Q4VAB2_MOUSE
resolver
activity_regulation
ACTIVITY REGULATION: Adopts a GDP-bound closed conformation and a GTP-bound open conformation with a hydrophobic pocket and switch from one to another by the binding of RNAPII subunit-derived hydrophobic peptides that are located at subunit interfaces. Peptide binding is thought to allosterically alter the active site, decreasing GDP affinity, promoting GDP release, and increasing GTP affinity to facilitate GTP rebinding. {ECO:0000250|UniProtKB:P47122}.
uniprot
proteins
amino_acid_sequence
MAAPVAPSEPQASRAPQPPVCLLVLGMAGSGKTTFVQRLTGHLHNKGCPPYVINLDPAVHEVPFPANIDIRDTVKYKEVMKQYGLGPNGGIVTSLNLFATRFDQVMKFIEKAQNTFRYVLIDTPGQIEVFTWSASGTIITEALASSFPTVVIYVMDTSRSTNPVTFMSNMLYACSILYKTKLPFIVVMNKTDIIDHSFAVEWMQDFEAFQDALNQETTYVSNLTRSMSLVLDEFYSSLRVVGVSAVVGTGFDELCTQVTSAAEEYEREYRPEYERLKKSLANAQSNQQKEQLERLRKDMGSVALDPEAGKGNASPVLDPSDLILTRGTLDEEDEEADSDTDDIDHRVTEESREEPAFQNFMEESMAHWKRNK
uniprot
proteins
ec_number
3.6.5.-
uniprot
proteins
function
FUNCTION: Small GTPase involved in the correct assembly of RNA polymerase II (RNAPII) complex, ensuring proper nuclear import of RNAPII (By similarity). In vitro, exhibits a chaperone-like activity and a chaperone substrate protein can stimulate its GTPase activity. It is proposed to bind exposed hydrophobic peptide regions of newly synthesized RNAP II subunit, triggering the opening of a hydrophobic pocket in its GDP-bound state. This interaction likely traps exposed hydrophobic regions, preventing misassembly and providing a time window for association with the cognate RNAP II subunit. Peptide binding promotes GDP release, and increasing GTP affinity to facilitate GTP rebinding. Subsequent GTP hydrolysis would then lead to the release of the bound RNAP II subunit to enable association with cognate subunits and RNAP II assembly (By similarity). Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. May be involved in nuclear localization of XPA (By similarity). {ECO:0000250|UniProtKB:P47122, ECO:0000250|UniProtKB:Q9HCN4}.
uniprot
proteins
mass_dalton
41598
uniprot
proteins
protein_family
GPN-loop GTPase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17242355
uniprot
proteins
pubmed
21183079
uniprot
proteins
sequence_length
372
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9HCN4}. Nucleus {ECO:0000250|UniProtKB:Q9HCN4}. Note=Shuttles with GPN3 between the nucleus and the cytoplasm. {ECO:0000250|UniProtKB:Q9HCN4}.
uniprot
proteins