Clnk
entrez
27278
canonical
uniprot
Q9QZE2
canonical
ensembl
ENSMUSG00000039315
resolver
ensp
ENSMUSP00000128473
resolver
genesymbol
Clnk
resolver
genesymbol_syn
Mist
resolver
uniprot
Q8C479
resolver
uniprot
Q9JMJ3
resolver
uniprot_entry
CLNK_MOUSE
resolver
uniprot_sec
Q8C479
resolver
uniprot_sec
Q9JMJ3
resolver
amino_acid_sequence
MTSQGNKRTTKEGFGDLRFQNVSLLKNRSWPSLSSAKGRCRAVLEPLPDHRRNLAGVPGGEKCNSNNDYEDPEFQLLKAWPSMKILPARPIQESEYADTRYFQDTMEAPLLLPPKASVSTERQTRDVRMTHLEEVDKPTFKDVRSQRFKGFKYTKINKTPLPPPRPAITLPKKYQPLPPAPPEESSAYFAPKPTFPEVQRGPRQRSAKDFSRVLGAEEESHHQTKPESSCPSSNQNTQKSPPAIASSSYMPGKHSIQARDHTGSMQHCPAQRCQAAASHSPRMLPYENTNSEKPDPTKPDEKDVWQNEWYIGEYSRQAVEDVLMKENKDGTFLVRDCSTKSKAEPYVLVVFYGNKVYNVKIRFLESNQQFALGTGLRGNEMFDSVEDIIEHYTYFPILLIDGKDKAARRKQCYLTQPLPLARLLLTQYSSQALHE
uniprot
proteins
function
FUNCTION: An adapter protein which plays a role in the regulation of immunoreceptor signaling, including PLC-gamma-mediated B-cell antigen receptor (BCR) signaling and FC-epsilon R1-mediated mast cell degranulation (PubMed:10562326, PubMed:10744659, PubMed:11509653, PubMed:12681493). Together with FGR, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production (PubMed:15199160, PubMed:16439675). Acts as a positive regulator of both T-cell receptor and natural killer T (NKT) cell receptor signaling in CD4-positive NKT cells (PubMed:16439675). Together with MAP4K1, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (PubMed:11509653). May be involved in tumor necrosis factor induced cell death by promoting reactive oxidative species generation, and MLKL oligomerization, ultimately leading to necrosis (PubMed:26009488). Involved in phosphorylation of LAT (PubMed:11463797). May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells (PubMed:12681493). {ECO:0000269|PubMed:10562326, ECO:0000269|PubMed:10744659, ECO:0000269|PubMed:11463797, ECO:0000269|PubMed:11509653, ECO:0000269|PubMed:12681493, ECO:0000269|PubMed:15199160, ECO:0000269|PubMed:16439675, ECO:0000269|PubMed:26009488}.
uniprot
proteins
mass_dalton
49492
uniprot
proteins
mutagenesis
MUTAGEN 69; /note="Y->F: Loss of phosphorylation and loss of interaction with PLCG1, PLCG2 and VAV. No effect on the suppression of natural killer cell activation and suppression of interferon-gamma production; when associated with F-96, F-101, F-153, F-174 and F-188."; /evidence="ECO:0000269|PubMed:10744659, ECO:0000269|PubMed:11463797, ECO:0000269|PubMed:16439675"; MUTAGEN 96; /note="Y->F: Loss of phosphorylation and loss of interaction with PLCG1, PLCG2 and VAV. No effect on the suppression of natural killer cell activation and suppression of interferon-gamma production; when associated with F-69, F-101, F-153, F-174 and F-188."; /evidence="ECO:0000269|PubMed:10744659, ECO:0000269|PubMed:11463797, ECO:0000269|PubMed:16439675"; MUTAGEN 101; /note="Y->F: Loss of phosphorylation and loss of interaction with PLCG1, PLCG2 and VAV. No effect on the suppression of natural killer cell activation and suppression of interferon-gamma production; when associated with F-69, F-96, F-153, F-174 and F-188."; /evidence="ECO:0000269|PubMed:10744659, ECO:0000269|PubMed:11463797, ECO:0000269|PubMed:16439675"; MUTAGEN 153; /note="Y->F: Loss of phosphorylation and loss of interaction with PLCG1, PLCG2 and VAV. No effect on the suppression of natural killer cell activation and suppression of interferon-gamma production; when associated with F-69, F-96, F-101, F-174 and F-188."; /evidence="ECO:0000269|PubMed:10744659, ECO:0000269|PubMed:11463797, ECO:0000269|PubMed:16439675"; MUTAGEN 160..165; /note="Missing: Slightly increases natural killer cell activation and interferon-gamma production."; /evidence="ECO:0000269|PubMed:16439675"; MUTAGEN 174; /note="Y->F: Loss of phosphorylation and loss of interaction with PLCG1, PLCG2 and VAV. No effect on the suppression of natural killer cell activation and suppression of interferon-gamma production; when associated with F-69, F-96, F-101, F-153 and F-188."; /evidence="ECO:0000269|PubMed:10744659, ECO:0000269|PubMed:11463797, ECO:0000269|PubMed:16439675"; MUTAGEN 178..182; /note="Missing: Increases natural killer cell activation and interferon-gamma production. Loss of FGR binding."; /evidence="ECO:0000269|PubMed:16439675"; MUTAGEN 188; /note="Y->F: Loss of phosphorylation and loss of interaction with PLCG1, PLCG2 and VAV. No effect on the suppression of natural killer cell activation and suppression of interferon-gamma production; when associated with F-69, F-96, F-101, F-153 and F-174."; /evidence="ECO:0000269|PubMed:10744659, ECO:0000269|PubMed:11463797, ECO:0000269|PubMed:16439675"; MUTAGEN 335; /note="R->K: Loss of binding to FYB1 and MAP4K1. Reduced tyrosine phosphorylation. No effect on the suppression of natural killer cell activation and suppression of interferon-gamma production."; /evidence="ECO:0000269|PubMed:12681493, ECO:0000269|PubMed:16439675
uniprot
proteins
post_translational_modification
PTM: Tyrosine-phosphorylated upon BCR cross-linking (PubMed:10744659, PubMed:11463797). Tyrosine phosphorylation at both Tyr-69 and Tyr-96 are required for BCR-induced calcium response and are essential to restore PLCG2-mediated signaling in BLNK-deficient DT40 cells, but this phosphorylation is dispensable in cells expressing LAT (PubMed:10744659, PubMed:11463797). Interacts with the SH2 domain of PLCG1 via phosphorylated Tyr-96 (PubMed:10744659, PubMed:11463797). Tyrosine phosphorylation is increased when complexed with SKAP1 and FYB1 (PubMed:12681493). {ECO:0000269|PubMed:10744659, ECO:0000269|PubMed:11463797, ECO:0000269|PubMed:12681493}.
uniprot
proteins
pubmed
10562326
uniprot
proteins
pubmed
10744659
uniprot
proteins
pubmed
11463797
uniprot
proteins
pubmed
11509653
uniprot
proteins
pubmed
12681493
uniprot
proteins
pubmed
15199160
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16439675
uniprot
proteins
pubmed
17947660
uniprot
proteins
pubmed
26009488
uniprot
proteins
sequence_length
435
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q7Z7G1}.
uniprot
proteins