Mrtfa
entrez
223701
canonical
uniprot
Q8K4J6
canonical
ensembl
ENSMUSG00000042292
resolver
ensp
ENSMUSP00000105207
resolver
ensp
ENSMUSP00000119530
resolver
ensp
ENSMUSP00000159052
resolver
enst
ENSMUST00000134469
resolver
enst
ENSMUST00000239114
resolver
genesymbol
Mrtfa
resolver
genesymbol_syn
Bsac
resolver
genesymbol_syn
Mal
resolver
genesymbol_syn
Mkl1
resolver
uniprot
A0A5F8MQ33
resolver
uniprot
Q642U1
resolver
uniprot_entry
A0A5F8MQ33_MOUSE
resolver
uniprot_entry
MRTFA_MOUSE
resolver
uniprot_sec
Q642U1
resolver
amino_acid_sequence
MTLLEPEMLMMAVQSVLQLKLQQRRTREELVSQGIMPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERAELVRMHILEETSAEPSLQAKQLKLKRARLADDLNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSPLESRVSDPLPSATSISPTQVLSQLPMAPDPGETLFLAEQPPLPPAPLLPPSLANGSIVPTAKPAPTLIKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDKGAPAMDSSYAKILQQQQLFLQLQILNQQQQQQQQQHYNYQAILPAPPKPSAETPGSSAPTPSRSLSTSSSPSSGTPGPSGLARQSSTALAAKPGALPANLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQVSPAPGAPKAPATTSVLSKAGEVVVAFPAALLSTGSALVTAGLAPAEMVVATVTSNGMVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDAFGEMVTSPLTQLTLQASPLQIVKEEGARAASCCLSPGARAELEGLDKDQMLQEKDKQIEELTRMLQQKQQLVELLRLQLEQQKRAQQPAPASSPVKRESGFSSCQLSCQPQGSAHAFGSGLVVPTTNHGDTQAPAPESPPVVVKQEAGPPEPDLAPSSQLLLGSQGTSFLKRVSPPTLVTDSTGTHLILTVTNKSADGPGLPAGSPQQPLSQPGSPAPGPPAQMDLEHPPQPPFATPTSLLKKEPPGYEETVTQQPKQQENGSSSQHMDDLFDILIQSGEISADFKEPPSLPGKEKSPPAAAAYGPPLTPQPSPLSELPQAAPPPGSPTLPGRLEDFLESSTGLPLLTSGHEGPEPLSLIDDLHSQMLSSSAILDHPPSPMDTSELHFAPEPSSGMGLDLAVGHLDSMDWLELSSGGPVLSLAPLSTAAPSLFSMDFLDGHDLQLHWDSCL
uniprot
proteins
function
FUNCTION: Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12019265, PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex (PubMed:12732141, PubMed:17588931). Activity is also regulated by filamentous actin (F-actin) in the nucleus (PubMed:23558171, PubMed:25759381). {ECO:0000269|PubMed:12019265, ECO:0000269|PubMed:12732141, ECO:0000269|PubMed:17588931, ECO:0000269|PubMed:19350017, ECO:0000269|PubMed:23558171, ECO:0000269|PubMed:24732378, ECO:0000269|PubMed:25759381}.
uniprot
proteins
mass_dalton
102546
uniprot
proteins
mutagenesis
MUTAGEN 24; /note="R->A: In 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-68 and A-112."; /evidence="ECO:0000269|PubMed:17588931"; MUTAGEN 45; /note="F->A,D: Induces a nuclear accumulation in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 48; /note="Q->A,D: Induces a nuclear accumulation in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 52; /note="L->A,D: Induces a nuclear accumulation in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 54; /note="R->A: Impaired interaction with G-actin, leading to nuclear accumulation in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 56; /note="R->A: Impaired interaction with G-actin, leading to nuclear accumulation in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 62..64; /note="KRK->AAA: Impaired localization to the nucleus."; /evidence="ECO:0000269|PubMed:20818336"; MUTAGEN 68; /note="R->A: In 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-24 and A-112."; /evidence="ECO:0000269|PubMed:17588931"; MUTAGEN 89; /note="L->A,D: Does not induce a nuclear accumulation in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 92; /note="K->A,D: Does not induce a nuclear accumulation in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 95..97; /note="KLK->AAA: Impaired localization to the nucleus."; /evidence="ECO:0000269|PubMed:20818336"; MUTAGEN 96; /note="L->A: Induces a nuclear accumulation in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 96; /note="L->D: Induces a nuclear decrease in unstimulated cells."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 98; /note="R->A: Impaired interaction with G-actin, leading to cytoplasmic accumulation."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 100; /note="R->A: Impaired interaction with G-actin, leading to cytoplasmic accumulation."; /evidence="ECO:0000269|PubMed:21673315"; MUTAGEN 112; /note="R->A: In 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-24 and A-68."; /evidence="ECO:0000269|PubMed:17588931
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Ser-41 by Erk inhibits binding of globular actin (G-actin), unmasking the nuclear localization signal (NLS) and promoting nuclear import. {ECO:0000269|PubMed:27304076}.
uniprot
proteins
pubmed
12019265
uniprot
proteins
pubmed
12397177
uniprot
proteins
pubmed
12732141
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17588931
uniprot
proteins
pubmed
19008859
uniprot
proteins
pubmed
19350017
uniprot
proteins
pubmed
20818336
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21673315
uniprot
proteins
pubmed
23558171
uniprot
proteins
pubmed
24732378
uniprot
proteins
pubmed
25759381
uniprot
proteins
pubmed
27304076
uniprot
proteins
sequence_length
964
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12732141, ECO:0000269|PubMed:17588931, ECO:0000269|PubMed:19008859, ECO:0000269|PubMed:21673315, ECO:0000269|PubMed:25759381, ECO:0000269|PubMed:27304076}. Nucleus {ECO:0000269|PubMed:12019265, ECO:0000269|PubMed:12732141, ECO:0000269|PubMed:17588931, ECO:0000269|PubMed:19008859, ECO:0000269|PubMed:20818336, ECO:0000269|PubMed:21673315, ECO:0000269|PubMed:25759381, ECO:0000269|PubMed:27304076}. Note=Subcellular location is tightly regulated by actin both in cytoplasm and nucleus: high levels of G-actin in the nucleus observed during serum deprivation lead to low levels of nuclear MRTFA, while reduced levels of nuclear G-actin result in accumulation of MRTFA in the nucleus (PubMed:17588931, PubMed:21673315). G-actin-binding in the cytoplasm inhibits nuclear import by masking the nuclear localization signal (NLS) (PubMed:17588931, PubMed:20818336, PubMed:21673315). In contrast, binding to nuclear globular actin (G-actin) promotes nuclear export to the cytoplasm (PubMed:17588931). Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear actin, which decreases nuclear G-actin pool, thereby promoting retention of MRTFA in the nucleus and subsequent formation of an active complex with SRF (By similarity). Nuclear import is mediated by importins KPNA4 and KPNB1 (PubMed:20818336). {ECO:0000250|UniProtKB:Q969V6, ECO:0000269|PubMed:17588931, ECO:0000269|PubMed:20818336, ECO:0000269|PubMed:21673315}.
uniprot
proteins