Spef1
entrez
70997
canonical
uniprot
Q99JL1
canonical
ensembl
ENSMUSG00000027329
resolver
ensp
ENSMUSP00000028801
resolver
ensp
ENSMUSP00000105847
resolver
enst
ENSMUST00000028801
resolver
enst
ENSMUST00000110218
resolver
genesymbol
Spef1
resolver
genesymbol_syn
Clamp
resolver
uniprot
A2ANS9
resolver
uniprot
Q543S5
resolver
uniprot
Q9D4J8
resolver
uniprot_entry
SPEF1_MOUSE
resolver
uniprot_sec
A2ANS9
resolver
uniprot_sec
Q543S5
resolver
uniprot_sec
Q9D4J8
resolver
amino_acid_sequence
MESSVDEEALHQLYLWVDNIPLSRPKRNLSRDFSDGVLVAELIKFYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLNKLNFSVPDDVMRKIAQCSPGVVELVLIPLRQRLEERQRRQKLGVGSLQELAPQDSSGYMDMGLPQKVRGEGAPALGEQLREGRPLASRPPGYNQALQGDPSFVLQIAEKEQELLASQETVQVLQMKVKRLEHLLQLKNVRIDDLSRRLQQAERKQR
uniprot
proteins
function
FUNCTION: Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (PubMed:16206169). Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity). Essential for ciliary central apparatus formation which requires both its microtubule-binding and bundling activities and for ciliary localization of HYDIN and SPAG6 in ependymal cilia (PubMed:30535028). Binds actin in intestinal epithelial cells (IECs), essential for IECs survival and contributes to formation of filopodia and lamellipodia in migrating IECs (By similarity). Regulates planar cell polarity signaling pathway and asymmetric microtubule accumulation in ciliated epithelia (By similarity). {ECO:0000250|UniProtKB:Q0IH24, ECO:0000269|PubMed:16206169, ECO:0000269|PubMed:30535028}.
uniprot
proteins
mass_dalton
26839
uniprot
proteins
mutagenesis
MUTAGEN 31; /note="R->A: Disruption of its microtubule-binding and microtubule bundling activities. Failure to rescue ciliary central apparatus formation in SPEF1-depleted ependymal cilia."; /evidence="ECO:0000269|PubMed:30535028
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15979255
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16206169
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
30535028
uniprot
proteins
sequence_length
234
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15979255}. Cell projection, cilium, flagellum {ECO:0000269|PubMed:15979255}. Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000269|PubMed:30535028}. Apical cell membrane {ECO:0000250|UniProtKB:Q0IH24}. Basolateral cell membrane {ECO:0000250|UniProtKB:Q9Y4P9}. Cytoplasm, cytoskeleton, stress fiber {ECO:0000250|UniProtKB:Q9Y4P9}. Cell projection, microvillus {ECO:0000250|UniProtKB:Q9Y4P9}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:Q9Y4P9}. Cell projection, filopodium {ECO:0000250|UniProtKB:Q9Y4P9}. Note=Also found at the apical tip of cilia (PubMed:30535028). Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum (PubMed:15979255). Colocalizes with TJP1 and CGN at sites of cell-cell contact in intestinal epithelial cells (By similarity). {ECO:0000250|UniProtKB:Q9Y4P9, ECO:0000269|PubMed:15979255, ECO:0000269|PubMed:30535028}.
uniprot
proteins