Arf6
entrez
11845
canonical
uniprot
P62331
canonical
ensembl
ENSMUSG00000044147
resolver
ensp
ENSMUSP00000055862
resolver
enst
ENSMUST00000050063
resolver
genesymbol
Arf6
resolver
uniprot
P26438
resolver
uniprot
Q3U0D7
resolver
uniprot_entry
ARF6_MOUSE
resolver
uniprot_entry
Q3U0D7_MOUSE
resolver
uniprot_sec
P26438
resolver
activity_regulation
ACTIVITY REGULATION: Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by GTPases activating protein (GAP). Inactivated by ACAP1 and ACAP2 (By similarity). Activated by NGF via NTRK1 (By similarity). {ECO:0000250|UniProtKB:P62330, ECO:0000250|UniProtKB:P62332}.
uniprot
proteins
amino_acid_sequence
MGKVLSKIFGNKEMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLTSNYKS
uniprot
proteins
ec_number
3.6.5.2
uniprot
proteins
function
FUNCTION: GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11950392). Required for normal completion of mitotic cytokinesis. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (PubMed:20080746, PubMed:29420262). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity). {ECO:0000250|UniProtKB:P62332, ECO:0000269|PubMed:11950392, ECO:0000269|PubMed:16325184, ECO:0000269|PubMed:20080746, ECO:0000269|PubMed:22522702, ECO:0000269|PubMed:23572513, ECO:0000269|PubMed:29420262}.
uniprot
proteins
mass_dalton
20082
uniprot
proteins
mutagenesis
MUTAGEN 27; /note="T->N: Loss of activity; delays formation of epithelial polarity."; /evidence="ECO:0000269|PubMed:20080746, ECO:0000269|PubMed:22522702"; MUTAGEN 44; /note="T->N: Inactive GDP-bound form which does not bind TJAP1."; /evidence="ECO:0000269|PubMed:22841714"; MUTAGEN 67; /note="Q->L: Probable constitutively active mutant that prevents EHD1 localization to endosome membranes. No effect on interaction with TJAP1."; /evidence="ECO:0000269|PubMed:22522702, ECO:0000269|PubMed:22841714, ECO:0000269|PubMed:23572513"; MUTAGEN 76; /note="H->A: Slightly impaired interaction with KIF23. Abolishes interaction with GGA1, SPAG9 and RAB11FIP4."; /evidence="ECO:0000269|PubMed:22522702"; MUTAGEN 77; /note="Y->A: Loss of interaction with KIF23, GGA1, SPAG9 and RAB11FIP4."; /evidence="ECO:0000269|PubMed:22522702
uniprot
proteins
post_translational_modification
PTM: GTP-bound form is myristoylated on Lys-3 by NMT1 and NMT2, allowing ARF6 to remain on membranes during the GTPase cycle, thereby promoting its activity. GDP-bound inactive form is demyristoylated on Lys-3 by SIRT2 at early endosomes or endocytic recycling compartment to allow its efficient activation by a guanine exchange factor (GEF) after GDP release. {ECO:0000250|UniProtKB:P62330}.
uniprot
proteins
protein_family
Arf family
uniprot
proteins
protein_family
Small GTPase superfamily
uniprot
proteins
pubmed
11950392
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16325184
uniprot
proteins
pubmed
20080746
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22522702
uniprot
proteins
pubmed
22841714
uniprot
proteins
pubmed
23572513
uniprot
proteins
pubmed
27723745
uniprot
proteins
pubmed
29420262
uniprot
proteins
pubmed
8947846
uniprot
proteins
sequence_length
175
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P62330}. Cell membrane {ECO:0000269|PubMed:29420262}; Lipid-anchor {ECO:0000250|UniProtKB:P62330}. Endosome membrane; Lipid-anchor {ECO:0000250|UniProtKB:P62330}. Recycling endosome membrane {ECO:0000250|UniProtKB:P62330}; Lipid-anchor {ECO:0000250|UniProtKB:P62330}. Cell projection, filopodium membrane {ECO:0000250|UniProtKB:P62330}; Lipid-anchor {ECO:0000250|UniProtKB:P62330}. Cell projection, ruffle {ECO:0000250|UniProtKB:P62330}. Cleavage furrow {ECO:0000250|UniProtKB:P62330}. Midbody, Midbody ring {ECO:0000269|PubMed:22522702}. Early endosome membrane {ECO:0000269|PubMed:11950392}; Lipid-anchor {ECO:0000305}. Golgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:11950392}; Lipid-anchor {ECO:0000305}. Note=Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase (By similarity). Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression (By similarity). In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (By similarity). Recruitment to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1 (By similarity). After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (PubMed:22522702). Recruited to the cell membrane in association with CYTH2 and ARL4C (By similarity). Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles (By similarity). Myristoylation is required for proper localization to membranes: myristoylation on Lys-3 allows ARF6 to remain on membranes during the GTPase cycle (By similarity). {ECO:0000250|UniProtKB:P62330, ECO:0000269|PubMed:22522702}.
uniprot
proteins