Diaph3
entrez
56419
canonical
uniprot
Q9Z207
canonical
ensembl
ENSMUSG00000022021
resolver
ensp
ENSMUSP00000129420
resolver
enst
ENSMUST00000168889
resolver
genesymbol
Diaph3
resolver
genesymbol_syn
Diap3
resolver
uniprot_entry
DIAP3_MOUSE
resolver
amino_acid_sequence
MERHRARALGRDSKSSRRKGLQSAPPAGPYEPGEKRPKLHLNIRTLTDDMLDKFASIRIPGSKKERPPLPHLKTVSGISDSSSLSSETMENNPKALPESEVLKLFEKMMEDMNLNEDKKAPLREKDFGIKKEMVMQYINTASKTGSLRSSRQISPQEFLHELKMGYTDERLFTYLESLRVSLTSHPVSWVQSFGHEGLGLLLDILEKLINGQIQEKVVKKTQHKVIQCLRALMNTQYGLERIMSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHNSVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEFFHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRDGTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAELQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPPPPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLENTFCCQEKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESMIQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEKHADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSSFVISANEQYEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKERAEKERLERQQEKKRLLEMKTEGDETGVMDSLLEALQSGAAFRDRRKRTPKLKDIRQSLSPMSQRPVLKVCNHENQKMQLTEGSRPHHSINCNSTRTPVAKELNYNLDTHASTGRIKAVEKEACNAESNKKKEMELLGSVAKSESVPEVEALLARLRAL
uniprot
proteins
function
FUNCTION: Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:23558171). Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:23558171). Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization (PubMed:10678165). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171). {ECO:0000269|PubMed:10678165, ECO:0000269|PubMed:23558171}.
uniprot
proteins
mass_dalton
133686
uniprot
proteins
mutagenesis
MUTAGEN 18..19; /note="RK->AA: Decreased accumulation in the nucleus."; /evidence="ECO:0000269|PubMed:19117945"; MUTAGEN 35..36; /note="KR->AA: Abolished accumulation in the nucleus."; /evidence="ECO:0000269|PubMed:19117945"; MUTAGEN 118; /note="K->R: No loss of ubiquitination; when associated with R-119, R-493 and R-494."; /evidence="ECO:0000269|PubMed:19457867"; MUTAGEN 119; /note="K->R: No loss of ubiquitination; when associated with R-118, R-493 and R-494."; /evidence="ECO:0000269|PubMed:19457867"; MUTAGEN 268..269; /note="VK->AA: Does not affect subcellular location."; /evidence="ECO:0000269|PubMed:19117945"; MUTAGEN 274..277; /note="VCIV->ACIA: Does not affect subcellular location."; /evidence="ECO:0000269|PubMed:19117945"; MUTAGEN 493; /note="K->R: No loss of ubiquitination; when associated with R-118, R-119 and R-494."; /evidence="ECO:0000269|PubMed:19457867"; MUTAGEN 494; /note="K->R: No loss of ubiquitination; when associated with R-118, R-119 and R-493."; /evidence="ECO:0000269|PubMed:19457867"; MUTAGEN 1041; /note="M->A: Impaired ability to release autoinhibition, leading to defects in actin nucleation and elongation factor activity in the nucleus."; /evidence="ECO:0000269|PubMed:23558171"; MUTAGEN 1168; /note="L->G: Accumulation in the nucleus due to defects in nuclear export."; /evidence="ECO:0000269|PubMed:19117945, ECO:0000269|PubMed:23558171
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated. {ECO:0000269|PubMed:19457867}.
uniprot
proteins
protein_family
Diaphanous subfamily
uniprot
proteins
protein_family
Formin homology family
uniprot
proteins
pubmed
10678165
uniprot
proteins
pubmed
19117945
uniprot
proteins
pubmed
19457867
uniprot
proteins
pubmed
23558171
uniprot
proteins
pubmed
9535835
uniprot
proteins
sequence_length
1171
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19117945, ECO:0000269|PubMed:23558171}. Nucleus {ECO:0000269|PubMed:19117945, ECO:0000269|PubMed:23558171}. Note=During mitosis, co-localizes with the actin-rich cleavage furrow and with the microtubule-rich central spindle during cytokinesis (By similarity). Shuttles between the cytoplasm and the nucleus (PubMed:19117945). {ECO:0000250|UniProtKB:Q9NSV4, ECO:0000269|PubMed:19117945}.
uniprot
proteins