Q63744
uniprot
Q63744
canonical
amino_acid_sequence
MCRDEPDTMILTQIEAKEACDWLRVTGFPQYAQLYEDLLFPIDIALVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEEEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDNSRLQSATSRESMLTDLSEHQEVSSIRSLSSTSSSAPTHVPHSGEATTPRTNSVISVCSSSHFVGNEDSFSSLPSPKELSSFSFSMKGHEKNTKSKTRSLLKRMESLKLKGSHHSKHKAPSKLGLIISAPILQEGMDEEKLKQLNCVEISALNGNHINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSTCNKRVGMYLEGFDPFSQSAFNNVTEQNYKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFCPSGNSSVNWRTGSFHGPGHLSLRRENSSDSPKELKRRNSSSSVSSRMSIYDNVPGSILYSSSGELADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDHDRRTPSDLDSTGNSLNEPEEPTDIPERRDSGVGASLTRCNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRSVFGVPLTVNVQRSGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESAEDYVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSHVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLSNDQPADYRHFLQDCVDGLFKEVKEKFKGWVSYPTSEQAELSYKKVSEGPPLRLWRATIEVPAAPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPRGACALLFTSVDHDRAPVAGVRVNVLLSRYLIEPCGSGKSKLTYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQSTESKDTRSR
uniprot
proteins
function
FUNCTION: Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality (By similarity). Required for growth factor-induced epithelial cell migration; in resting cells, interacts with TNS3 while PTEN interacts with the p85 regulatory subunit of the PI3K kinase complex but growth factor stimulation induces phosphorylation of TNS3 and PTEN, causing them to change their binding preference so that PTEN interacts with DLC1 and TNS3 interacts with p85 (By similarity). The PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA while the TNS3-p85 complex translocates to the leading edge of migrating cells to promote RAC1 activation (By similarity). {ECO:0000250|UniProtKB:Q96QB1, ECO:0000269|PubMed:10364218}.
uniprot
proteins
mass_dalton
123414
uniprot
proteins
mutagenesis
MUTAGEN 677; /note="R->E: Loss of activity."; /evidence="ECO:0000269|PubMed:10364218"; MUTAGEN 714; /note="K->E: Loss of activity."; /evidence="ECO:0000269|PubMed:10364218"; MUTAGEN 718; /note="R->E: Loss of activity."; /evidence="ECO:0000269|PubMed:10364218"; MUTAGEN 787; /note="N->V: No loss of activity."; /evidence="ECO:0000269|PubMed:10364218
uniprot
proteins
pubmed
10364218
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
7835339
uniprot
proteins
sequence_length
1091
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q96QB1}. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Colocalizes with EF1A1 at actin-rich regions in the cell periphery. {ECO:0000250}.
uniprot
proteins