TRUB1
entrez
142940
canonical
uniprot
Q8WWH5
canonical
ensembl
ENSG00000165832
resolver
ensp
ENSP00000298746
resolver
enst
ENST00000298746
resolver
genesymbol
TRUB1
resolver
genesymbol_syn
PUS4
resolver
hgnc
HGNC:16060
resolver
uniprot
B2R716
resolver
uniprot
Q53ES2
resolver
uniprot_entry
TRUB1_HUMAN
resolver
uniprot_sec
B2R716
resolver
uniprot_sec
Q53ES2
resolver
amino_acid_sequence
MAASEAAVVSSPSLKTDTSPVLETAGTVAAMAATPSARAAAAVVAAAARTGSEARVSKAALATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTSMLSGSKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELALKKSKPESNEQVLSCEYITLNEPKREDDVIKTC
uniprot
proteins
ec_number
5.4.99.-
uniprot
proteins
ec_number
5.4.99.25
uniprot
proteins
function
FUNCTION: Pseudouridine synthase that catalyzes pseudouridylation of mRNAs and tRNAs (PubMed:28073919, PubMed:31477916, PubMed:32926445). Mediates pseudouridylation of mRNAs with the consensus sequence 5'-GUUCNANNC-3', harboring a stem-loop structure (PubMed:28073919, PubMed:31477916). Constitutes the major pseudouridine synthase acting on mRNAs (PubMed:28073919). Also catalyzes pseudouridylation of some tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the psi GC loop of a subset of tRNAs (PubMed:32926445, PubMed:33023933). Promotes the processing of pri-let-7 microRNAs (pri-miRNAs) independently of its RNA pseudouridylate synthase activity (PubMed:32926445). Acts by binding to the stem-loop structure on pri-let-7, preventing LIN28-binding (LIN28A and/or LIN28B), thereby enhancing the interaction between pri-let-7 and the microprocessor DGCR8, which mediates miRNA maturation (PubMed:32926445). {ECO:0000269|PubMed:28073919, ECO:0000269|PubMed:31477916, ECO:0000269|PubMed:32926445, ECO:0000269|PubMed:33023933}.
uniprot
proteins
mass_dalton
37253
uniprot
proteins
mutagenesis
MUTAGEN 121; /note="D->A: Abolished pseudouridine synthase activity without affecting ability to promote processing of let-7 miRNAs; when associated with A-163."; /evidence="ECO:0000269|PubMed:32926445"; MUTAGEN 147; /note="K->E: Abolished RNA-binding and ability to promote processing of let-7 miRNAs."; /evidence="ECO:0000269|PubMed:32926445"; MUTAGEN 163; /note="D->A: Abolished pseudouridine synthase activity without affecting ability to promote processing of let-7 miRNAs; when associated with A-121."; /evidence="ECO:0000269|PubMed:32926445
uniprot
proteins
protein_family
Pseudouridine synthase TruB family
uniprot
proteins
pubmed
12736709
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15164054
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
17974005
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22223895
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
28073919
uniprot
proteins
pubmed
31477916
uniprot
proteins
pubmed
32926445
uniprot
proteins
pubmed
33023933
uniprot
proteins
sequence_length
349
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28073919, ECO:0000269|PubMed:33023933}. Cytoplasm, cytosol {ECO:0000269|PubMed:28073919}. Note=Catalyzes pseudouridylation of mRNAs in the nucleus. {ECO:0000269|PubMed:28073919}.
uniprot
proteins