DGCR8
entrez
54487
canonical
uniprot
Q8WYQ5
canonical
ensembl
ENSG00000128191
resolver
ensp
ENSP00000263209
resolver
ensp
ENSP00000384726
resolver
ensp
ENSP00000538542
resolver
ensp
ENSP00000538547
resolver
genesymbol
DGCR8
resolver
genesymbol_syn
C22orf12
resolver
genesymbol_syn
DGCRK6
resolver
hgnc
HGNC:2847
resolver
uniprot
B2R8G1
resolver
uniprot
Q6DCB2
resolver
uniprot
Q6MZE9
resolver
uniprot
Q6Y2L0
resolver
uniprot
Q96G39
resolver
uniprot
Q96GP8
resolver
uniprot
Q9H6L8
resolver
uniprot
Q9H6T7
resolver
uniprot
Q9NRW2
resolver
uniprot_entry
DGCR8_HUMAN
resolver
uniprot_sec
B2R8G1
resolver
uniprot_sec
Q6DCB2
resolver
uniprot_sec
Q6MZE9
resolver
uniprot_sec
Q6Y2L0
resolver
uniprot_sec
Q96G39
resolver
uniprot_sec
Q96GP8
resolver
uniprot_sec
Q9H6L8
resolver
uniprot_sec
Q9H6T7
resolver
uniprot_sec
Q9NRW2
resolver
amino_acid_sequence
METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV
uniprot
proteins
function
FUNCTION: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity). Also plays a role in DNA repair by promoting the recruitment of RNF168 to RNF8 and MDC1 at DNA double-strand breaks and subsequently the clearance of DNA breaks (PubMed:34188037). {ECO:0000250|UniProtKB:Q9EQM6, ECO:0000269|PubMed:15531877, ECO:0000269|PubMed:15574589, ECO:0000269|PubMed:15589161, ECO:0000269|PubMed:16751099, ECO:0000269|PubMed:16906129, ECO:0000269|PubMed:16963499, ECO:0000269|PubMed:17159994, ECO:0000269|PubMed:25799998, ECO:0000269|PubMed:26027739, ECO:0000269|PubMed:26748718}.
uniprot
proteins
mass_dalton
86045
uniprot
proteins
mutagenesis
MUTAGEN 352; /note="C->A,H: Inhibits heme-binding and dimerization."; /evidence="ECO:0000269|PubMed:17159994"; MUTAGEN 430; /note="C->A: Does not inhibit heme-binding and dimerization."; /evidence="ECO:0000269|PubMed:17159994"; MUTAGEN 561..565; /note="KKLAK->AALAA: Strongly reduces pri-miRNA binding affinity."; /evidence="ECO:0000269|PubMed:17704815"; MUTAGEN 568..569; /note="AA->KK: Reduces pri-miRNA binding affinity and pri-miRNA processing activity. Does not inhibit interaction with DROSHA. When associated with A-676 and S-677, strongly reduces binding affinity and pri-miRNA processing activity."; /evidence="ECO:0000269|PubMed:16963499"; MUTAGEN 669..673; /note="KRVGK->AAVGA: Strongly reduces pri-miRNA binding affinity."; /evidence="ECO:0000269|PubMed:17704815"; MUTAGEN 676..677; /note="AS->KK: Reduces pri-miRNA binding affinity and pri-miRNA processing activity. Slightly inhibits interaction with DROSHA. When associated with A-568 and A-568, strongly reduces binding affinity and pri-miRNA processing activity."; /evidence="ECO:0000269|PubMed:16963499
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Ser-677 by ATM upon radiation, which is crucial for its stability. {ECO:0000269|PubMed:34188037}.; PTM: Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization. {ECO:0000269|PubMed:34188037}.
uniprot
proteins
pubmed
10591208
uniprot
proteins
pubmed
12705904
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15461802
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15498874
uniprot
proteins
pubmed
15531877
uniprot
proteins
pubmed
15574589
uniprot
proteins
pubmed
15589161
uniprot
proteins
pubmed
16751099
uniprot
proteins
pubmed
16906129
uniprot
proteins
pubmed
16963499
uniprot
proteins
pubmed
17081983
uniprot
proteins
pubmed
17159994
uniprot
proteins
pubmed
17704815
uniprot
proteins
pubmed
17765891
uniprot
proteins
pubmed
17974005
uniprot
proteins
pubmed
18220336
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
19135890
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
22118463
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
25218447
uniprot
proteins
pubmed
25755297
uniprot
proteins
pubmed
25799998
uniprot
proteins
pubmed
26027739
uniprot
proteins
pubmed
26748718
uniprot
proteins
pubmed
28112733
uniprot
proteins
pubmed
31819270
uniprot
proteins
pubmed
34188037
uniprot
proteins
sequence_length
773
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16906129, ECO:0000269|PubMed:16963499, ECO:0000269|PubMed:17159994, ECO:0000269|PubMed:22118463, ECO:0000269|PubMed:34188037}. Nucleus, nucleolus {ECO:0000269|PubMed:17159994}. Note=Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies (PubMed:17159994).
uniprot
proteins