Ano6
entrez
105722
canonical
uniprot
Q6P9J9
canonical
ensembl
ENSMUSG00000064210
resolver
ensp
ENSMUSP00000071770
resolver
ensp
ENSMUSP00000153954
resolver
enst
ENSMUST00000227791
resolver
genesymbol
Ano6
resolver
genesymbol_syn
Tmem16f
resolver
uniprot
A0A2I3BPX3
resolver
uniprot
Q8C242
resolver
uniprot_entry
A0A2I3BPX3_MOUSE
resolver
uniprot_entry
ANO6_MOUSE
resolver
uniprot_sec
Q8C242
resolver
activity_regulation
ACTIVITY REGULATION: Exhibits synergistic gating by Ca(2+) and voltage. Inhibited by some non-specific cation channel blockers such as: ruthenium red, 2-aminoethyl diphenylborinate (2APB), gadolinium and cadmium ions. {ECO:0000269|PubMed:23021219}.
uniprot
proteins
amino_acid_sequence
MQMMTRKVLLNMELEEDDDEDGDIVLENFDQTIVCPTFGSLENQQDFRTPEFEEFNGKPDSLFFTDGQRRIDFILVYEDESKKENNKKGTNEKQKRKRQAYESNLICHGLQLEATRSVSDDKLVFVKVHAPWEVLCTYAEIMHIKLPLKPNDLKTRSPFGNLNWFTKVLRVNESVIKPEQEFFTAPFEKSRMNDFYILDRDSFFNPATRSRIVYFILSRVKYQVMNNVNKFGINRLVSSGIYKAAFPLHDCRFNYESEDISCPSERYLLYREWAHPRSIYKKQPLDLIRKYYGEKIGIYFAWLGYYTQMLLLAAVVGVACFLYGYLDQDNCTWSKEVCDPDIGGQILMCPQCDRLCPFWRLNITCESSKKLCIFDSFGTLIFAVFMGVWVTLFLEFWKRRQAELEYEWDTVELQQEEQARPEYEAQCNHVVINEITQEEERIPFTTCGKCIRVTLCASAVFFWILLIIASVIGIIVYRLSVFIVFSTTLPKNPNGTDPIQKYLTPQMATSITASIISFIIIMILNTIYEKVAIMITNFELPRTQTDYENSLTMKMFLFQFVNYYSSCFYIAFFKGKFVGYPGDPVYLLGKYRSEECDPGGCLLELTTQLTIIMGGKAIWNNIQEVLLPWVMNLIGRYKRVSGSEKITPRWEQDYHLQPMGKLGLFYEYLEMIIQFGFVTLFVASFPLAPLLALVNNILEIRVDAWKLTTQFRRMVPEKAQDIGAWQPIMQGIAILAVVTNAMIIAFTSDMIPRLVYYWSFSIPPYGDHTYYTMDGYINNTLSVFNITDFKNTDKENPYIGLGNYTLCRYRDFRNPPGHPQEYKHNIYYWHVIAAKLAFIIVMEHIIYSVKFFISYAIPDVSKITKSKIKREKYLTQKLLHESHLKDLTKNMGIIAERIGGTVDNSVRPKLE
uniprot
proteins
function
FUNCTION: Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes (PubMed:21107324, PubMed:21908539, PubMed:22936354, PubMed:23021219, PubMed:23532839, PubMed:30785399, PubMed:31015464, PubMed:39495104). Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization (PubMed:22936354, PubMed:23021219). Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (PubMed:23532839). Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes (PubMed:23021219). {ECO:0000269|PubMed:21107324, ECO:0000269|PubMed:21908539, ECO:0000269|PubMed:22936354, ECO:0000269|PubMed:23021219, ECO:0000269|PubMed:23532839, ECO:0000269|PubMed:30785399, ECO:0000269|PubMed:31015464, ECO:0000269|PubMed:39495104}.
uniprot
proteins
mass_dalton
106255
uniprot
proteins
mutagenesis
MUTAGEN 370; /note="K->A: No effect on lipid scramblase activity."; /evidence="ECO:0000269|PubMed:30785399"; MUTAGEN 409; /note="D->G: Increased speed of phospholipid scrambling."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 409; /note="D->G: Reduced channel activity and sensitivity to Ca(2+)."; /evidence="ECO:0000269|PubMed:23021219"; MUTAGEN 478; /note="R->A: Decreased lipid scramblase and ion channel activity. Requires lower calcium levels for activation of ion channel activity."; /evidence="ECO:0000269|PubMed:30785399"; MUTAGEN 518; /note="F->A: Increased speed of phospholipid scrambling. Constitutive scramblase activity at basal cytosolic calcium levels; when associated with A-563 and A-612."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 518; /note="F->E,K,Q: Constitutive phospholipid scramblase activity at basal cytosolic calcium levels."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 518; /note="F->L: Decreased lipid scramblase activity."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 518; /note="F->W: Slightly increased lipid scramblase activity."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 521; /note="I->A: Does not induce a constitutive phospholipid scramblase activity."; /evidence="ECO:0000269|PubMed:39495104"; MUTAGEN 521; /note="I->K,E: Induces a constitutive phospholipid scramblase activity."; /evidence="ECO:0000269|PubMed:39495104"; MUTAGEN 522; /note="M->K: Induces a constitutive phospholipid scramblase activity."; /evidence="ECO:0000269|PubMed:39495104"; MUTAGEN 526; /note="T->K: Induces a constitutive phospholipid scramblase activity."; /evidence="ECO:0000269|PubMed:39495104"; MUTAGEN 559; /note="Q->K: Moderately decreased sensitivity to activation by calcium."; /evidence="ECO:0000269|PubMed:30785399"; MUTAGEN 559; /note="Q->K: Slower channel activation. Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:23021219"; MUTAGEN 563; /note="Y->A: Increased speed of phospholipid scrambling. Requires lower calcium levels for activation of scramblase and ion channel activity. Constitutive scramblase activity at basal cytosolic calcium levels; when associated with A-518 and A-612."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 563; /note="Y->K,Q: Constitutive phospholipid scramblase activity at basal cytosolic calcium levels."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 563; /note="Y->S: No effect on lipid scramblase activity."; /evidence="ECO:0000269|PubMed:30785399"; MUTAGEN 563; /note="Y->W: Loss of lipid scramblase activity."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 611; /note="I->K: Induces a constitutive phospholipid scramblase activity."; /evidence="ECO:0000269|PubMed:39495104"; MUTAGEN 612; /note="I->A: Increased speed of phospholipid scrambling. Constitutive scramblase activity at basal cytosolic calcium levels; when associated with A-518 and A-563."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 612; /note="I->E,K: Constitutive phospholipid scramblase activity at basal cytosolic calcium levels."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 612; /note="I->W: Decreased lipid scramblase activity."; /evidence="ECO:0000269|PubMed:31015464"; MUTAGEN 615; /note="G->A: Requires lower calcium levels for activation of scramblase and ion channel activity."; /evidence="ECO:0000269|PubMed:30785399"; MUTAGEN 623; /note="Q->F: No effect on lipid scramblase activity."; /evidence="ECO:0000269|PubMed:30785399"; MUTAGEN 624; /note="E->Q: Expected to disrupt calcium binding. Loss of scramblase and ion channel activity."; /evidence="ECO:0000269|PubMed:30785399"; MUTAGEN 667; /note="E->Q: Requires much higher calcium levels for the activation of scramblase and ion channel activity."; /evidence="ECO:0000269|PubMed:23021219, ECO:0000269|PubMed:30785399"; MUTAGEN 703; /note="D->R: Expected to disrupt calcium binding. Loss of scramblase activity."; /evidence="ECO:0000269|PubMed:31015464
uniprot
proteins
protein_family
Anoctamin family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
18729231
uniprot
proteins
pubmed
19656770
uniprot
proteins
pubmed
20056604
uniprot
proteins
pubmed
21107324
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21908539
uniprot
proteins
pubmed
22075693
uniprot
proteins
pubmed
22302790
uniprot
proteins
pubmed
22936354
uniprot
proteins
pubmed
23021219
uniprot
proteins
pubmed
23532839
uniprot
proteins
pubmed
30785399
uniprot
proteins
pubmed
31015464
uniprot
proteins
pubmed
39495104
uniprot
proteins
sequence_length
911
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:30785399}; Multi-pass membrane protein {ECO:0000269|PubMed:30785399}. Note=Shows an intracellular localization. {ECO:0000250|UniProtKB:Q4KMQ2}.
uniprot
proteins
transmembrane_region
TRANSMEM 302..322; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 377..397; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 457..477; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 511..531; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 553..573; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 603..622; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 665..685; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 686..706; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 724..744; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399"; TRANSMEM 838..858; /note="Helical"; /evidence="ECO:0000269|PubMed:30785399
uniprot
proteins