RNF25
entrez
64320
canonical
uniprot
Q96BH1
canonical
ensembl
ENSG00000163481
resolver
ensp
ENSP00000295704
resolver
enst
ENST00000295704
resolver
genesymbol
RNF25
resolver
hgnc
HGNC:14662
resolver
uniprot
A8K0D6
resolver
uniprot
Q53HQ5
resolver
uniprot
Q9H874
resolver
uniprot_entry
RNF25_HUMAN
resolver
uniprot_sec
A8K0D6
resolver
uniprot_sec
Q53HQ5
resolver
uniprot_sec
Q9H874
resolver
amino_acid_sequence
MAASASAAAGEEDWVLPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQYVCFTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFISLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQHICEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
function
FUNCTION: E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes (PubMed:36638793, PubMed:37651229, PubMed:37951216). Catalyzes ubiquitination of RPS27A in response to ribosome collisions, promoting activation of RNF14 (PubMed:36638793). RNF25 catalyzes ubiquitination of other ribosomal proteins on stalled ribosomes, such as RPL0, RPL1, RPL12, RPS13 and RPS17 (PubMed:36638793). Also involved in ubiquitination and degradation of stalled ETF1/eRF1 (PubMed:36638793, PubMed:37651229). Independently of its function in the response to stalled ribosomes, mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). May also stimulate transcription mediated by NF-kappa-B via its interaction with RELA/p65 (PubMed:12748188). {ECO:0000250|UniProtKB:Q9QZR0, ECO:0000269|PubMed:12748188, ECO:0000269|PubMed:36638793, ECO:0000269|PubMed:37651229, ECO:0000269|PubMed:37951216}.
uniprot
proteins
mass_dalton
51219
uniprot
proteins
mutagenesis
MUTAGEN 135..138; /note="CVIC->SVIS: Abolished E3 ubiquitin-protein ligase activity."; /evidence="ECO:0000269|PubMed:37651229"; MUTAGEN 137; /note="I->A: Strongly reduced E3 ubiquitin-protein ligase activity, slightly reduced binding to UBE2D2."; /evidence="ECO:0000269|PubMed:26475854"; MUTAGEN 159; /note="C->S: Reduced activation of NF-kappa-B."; /evidence="ECO:0000269|PubMed:12748188"; MUTAGEN 161; /note="C->S: Strongly reduced activation of NF-kappa-B."; /evidence="ECO:0000269|PubMed:12748188"; MUTAGEN 165; /note="Y->A: Decreased E3 ubiquitin-protein ligase activity, increased binding to UBE2D2."; /evidence="ECO:0000269|PubMed:26475854"; MUTAGEN 199; /note="P->A: Decreased E3 ubiquitin-protein ligase activity without affecting binding to UBE2D2."; /evidence="ECO:0000269|PubMed:26475854"; MUTAGEN 242; /note="Y->A: Decreased binding to UBE2D2."; /evidence="ECO:0000269|PubMed:26475854"; MUTAGEN 245; /note="Q->A: Decreased binding to UBE2D2."; /evidence="ECO:0000269|PubMed:26475854
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:26475854, ECO:0000269|PubMed:36638793, ECO:0000269|PubMed:37651229}.
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated; autoubiquitinated. {ECO:0000269|PubMed:26475854}.
uniprot
proteins
protein_family
RNF25 family
uniprot
proteins
pubmed
12748188
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15815621
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
26475854
uniprot
proteins
pubmed
36638793
uniprot
proteins
pubmed
37651229
uniprot
proteins
pubmed
37951216
uniprot
proteins
sequence_length
459
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q7SXJ6}.
uniprot
proteins