Nlrc4
entrez
268973
canonical
uniprot
Q3UP24
canonical
ensembl
ENSMUSG00000039193
resolver
ensp
ENSMUSP00000059637
resolver
ensp
ENSMUSP00000157167
resolver
enst
ENSMUST00000052124
resolver
genesymbol
Nlrc4
resolver
genesymbol_syn
Card12
resolver
genesymbol_syn
Ipaf
resolver
uniprot
Q3TAU8
resolver
uniprot_entry
NLRC4_MOUSE
resolver
uniprot_sec
Q3TAU8
resolver
amino_acid_sequence
MNFIRNNRRALIQRMGLTVTKQICDDLFALNVLNNQEANVIYCEPLEQEAARKIIHMTMQKGSAACNLFLKSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA
uniprot
proteins
function
FUNCTION: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. It senses pathogenic proteins of the type III secretion system (T3SS) and type IV secretion system (T4SS) such as flagellin and PrgJ-like rod proteins via the Naip proteins (Naip1, Naip2 or Naip5): specific Naip proteins recognize and bind pathogenic proteins, driving assembly and activation of the NLRC4 inflammasome. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri and fungal pathogen C.albicans. In intestine, the NLRC4 inflammasome is able to discriminate between commensal and pathogenic bacteria and specifically drives production of interleukin-1 beta (IL1B) in response to infection by Salmonella or P.aeruginosa. In case of L.pneumophila infection the inflammasome acts by activating caspase-7. {ECO:0000269|PubMed:15190255, ECO:0000269|PubMed:16648852, ECO:0000269|PubMed:16648853, ECO:0000269|PubMed:18070936, ECO:0000269|PubMed:19343209, ECO:0000269|PubMed:20133635, ECO:0000269|PubMed:20603313, ECO:0000269|PubMed:21874021, ECO:0000269|PubMed:21918512, ECO:0000269|PubMed:22174673, ECO:0000269|PubMed:22231517, ECO:0000269|PubMed:22484733, ECO:0000269|PubMed:22547706, ECO:0000269|PubMed:22885697, ECO:0000269|PubMed:29146805, ECO:0000269|PubMed:29182158}.
uniprot
proteins
mass_dalton
116749
uniprot
proteins
mutagenesis
MUTAGEN 443; /note="H->L: Constitutively active."; /evidence="ECO:0000269|PubMed:23765277"; MUTAGEN 533; /note="S->A: Abolishes phosphorylation and prevents activation of caspase-1 and pyroptosis in response to S.typhimurium."; /evidence="ECO:0000269|PubMed:22885697"; MUTAGEN 533; /note="S->D: Mimics phosphorylation; causes rapid macrophage pyroptosis without infection."; /evidence="ECO:0000269|PubMed:22885697
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro. {ECO:0000269|PubMed:22885697, ECO:0000269|PubMed:23765277}.
uniprot
proteins
pubmed
15190255
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16648852
uniprot
proteins
pubmed
16648853
uniprot
proteins
pubmed
16717117
uniprot
proteins
pubmed
18070936
uniprot
proteins
pubmed
19343209
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
20133635
uniprot
proteins
pubmed
20603313
uniprot
proteins
pubmed
21147462
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21874021
uniprot
proteins
pubmed
21918512
uniprot
proteins
pubmed
22174673
uniprot
proteins
pubmed
22231517
uniprot
proteins
pubmed
22484733
uniprot
proteins
pubmed
22547706
uniprot
proteins
pubmed
22885697
uniprot
proteins
pubmed
23765277
uniprot
proteins
pubmed
26449474
uniprot
proteins
pubmed
26585513
uniprot
proteins
pubmed
29146805
uniprot
proteins
pubmed
29182158
uniprot
proteins
sequence_length
1024
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:22885697}. Inflammasome {ECO:0000269|PubMed:21874021, ECO:0000269|PubMed:21918512, ECO:0000269|PubMed:26585513}.
uniprot
proteins