Gls
entrez
14660
canonical
uniprot
D3Z7P3
canonical
ensembl
ENSMUSG00000026103
resolver
ensp
ENSMUSP00000110155
resolver
ensp
ENSMUSP00000110157
resolver
ensp
ENSMUSP00000110158
resolver
ensp
ENSMUSP00000213829
resolver
genesymbol
Gls
resolver
genesymbol_syn
Gls1
resolver
genesymbol_syn
Kiaa0838
resolver
uniprot
E9PUF0
resolver
uniprot
Q69ZX9
resolver
uniprot_entry
GLSK_MOUSE
resolver
uniprot_sec
E9PUF0
resolver
uniprot_sec
Q69ZX9
resolver
activity_regulation
ACTIVITY REGULATION: Isoform 1 and isoform 2 are activated by phosphate, due to increased affinity for glutamine (PubMed:22228304, PubMed:23935106, PubMed:27542409). At phosphate concentrations above 10 mM, isoform 2 is more efficient than isoform 1. {ECO:0000269|PubMed:22228304, ECO:0000269|PubMed:23935106, ECO:0000269|PubMed:27542409}.
uniprot
proteins
amino_acid_sequence
MMRLRGSAMLRELLLRPPAAVGAVLRRAQPLGTLCRRPRGGSRPTAGLVAAARLHPWWGGGGRAKGPGAGGLSSSPSEILQELGKGGTPPQQQQQQQQQPGASPPAAPGPKDSPGETDAFGNSEGKEMVAAGDNKIKQGLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSIGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDDGKGNQTVHKNLDGLL
uniprot
proteins
ec_number
3.5.1.2
uniprot
proteins
function
FUNCTION: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. {ECO:0000269|PubMed:16641247, ECO:0000269|PubMed:22228304, ECO:0000269|PubMed:22373647}.
uniprot
proteins
mass_dalton
73964
uniprot
proteins
mutagenesis
MUTAGEN 202; /note="K->E: Increased stimulation of enzyme activity by phosphate."; /evidence="ECO:0000269|PubMed:23935106"; MUTAGEN 207; /note="K->E: Increased stimulation of enzyme activity by phosphate."; /evidence="ECO:0000269|PubMed:23935106"; MUTAGEN 254; /note="Y->F: Increased enzyme activity in the absence of phosphate. No effect on stimulation of enzyme activity by phosphate."; /evidence="ECO:0000269|PubMed:27542409"; MUTAGEN 316; /note="K->Q: Forms dimers with full, phosphate-independent activity; when associated with A-325 and K-391."; /evidence="ECO:0000269|PubMed:27542409"; MUTAGEN 320; /note="G->P: Loss of enzyme activity."; /evidence="ECO:0000269|PubMed:27542409"; MUTAGEN 325; /note="K->A: Constitutive enzyme activity that is fully active also in the absence phosphate. Forms oligomers with full, phosphate-independent activity; when associated with K-391. Forms dimers with full, phosphate-independent activity; when associated with Q-316 and K-391."; /evidence="ECO:0000269|PubMed:23935106, ECO:0000269|PubMed:27542409"; MUTAGEN 391; /note="D->K: Abolishes assembly of dimers into functional tetramers. Loss of enzyme activity. Forms oligomers with full, phosphate-independent activity; when associated with A-325. Forms dimers with full, phosphate-independent activity; when associated with Q-316 and A-325."; /evidence="ECO:0000269|PubMed:27542409"; MUTAGEN 394; /note="F->S: Impairs tetramerization and promotes formation of homodimers. Impairs activation by phosphate."; /evidence="ECO:0000269|PubMed:22228304
uniprot
proteins
post_translational_modification
PTM: Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits. {ECO:0000250|UniProtKB:P13264}.
uniprot
proteins
protein_family
Glutaminase family
uniprot
proteins
pubmed
15368895
uniprot
proteins
pubmed
16641247
uniprot
proteins
pubmed
16899818
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22228304
uniprot
proteins
pubmed
22373647
uniprot
proteins
pubmed
23806337
uniprot
proteins
pubmed
23935106
uniprot
proteins
pubmed
27542409
uniprot
proteins
sequence_length
674
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion {ECO:0000250|UniProtKB:P13264}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:P13264}. Note=The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits. {ECO:0000250|UniProtKB:P13264}.; SUBCELLULAR LOCATION: [Isoform 2]: Mitochondrion {ECO:0000269|PubMed:16641247, ECO:0000269|PubMed:27542409}.; SUBCELLULAR LOCATION: [Glutaminase kidney isoform, mitochondrial 68 kDa chain]: Mitochondrion matrix {ECO:0000250|UniProtKB:P13264}. Note=Produced by the proteolytic processing of the 74-kDa cytosolic precursor. {ECO:0000250|UniProtKB:P13264}.; SUBCELLULAR LOCATION: [Glutaminase kidney isoform, mitochondrial 65 kDa chain]: Mitochondrion matrix {ECO:0000250|UniProtKB:P13264}. Note=Produced by the proteolytic processing of the 74-kDa cytosolic precursor. {ECO:0000250|UniProtKB:P13264}.
uniprot
proteins