Spo11
entrez
26972
canonical
uniprot
Q9WTK8
canonical
ensembl
ENSMUSG00000005883
resolver
ensp
ENSMUSP00000059056
resolver
ensp
ENSMUSP00000104753
resolver
ensp
ENSMUSP00000104754
resolver
ensp
ENSMUSP00000357808
resolver
genesymbol
Spo11
resolver
uniprot
Q8VIH6
resolver
uniprot
Q9QUK2
resolver
uniprot
Q9QY57
resolver
uniprot
Q9QZS1
resolver
uniprot_entry
SPO11_MOUSE
resolver
uniprot_sec
Q8VIH6
resolver
uniprot_sec
Q9QUK2
resolver
uniprot_sec
Q9QY57
resolver
uniprot_sec
Q9QZS1
resolver
activity_regulation
ACTIVITY REGULATION: Homodimerization transforms the enzyme from an inactive monomer into a functional dimer capable of catalyzing programmed DNA double-strand breaks (DSBs) to initiate meiosis. {ECO:0000269|PubMed:39972125, ECO:0000269|PubMed:39972129, ECO:0000269|PubMed:39972130}.
uniprot
proteins
amino_acid_sequence
MAFAPMGPEASFFDALDRHRASLLAMVKRGAGETPAGATRVASSSEVLTAIENIIQDIIKSLARNEVPAFTIDNRSSWENIMFDDSVGLRMIPQCTTRKIRSDSPKSVKKFALILKVLSMIYKLIQSDTYATKRDIYYTDSQLFGNQAAVDSAIDDISCMLKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSATATAVPTNIQGMQHLITDAKFLLIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIPVFTLVDADPYGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNIPKDSLIPLTKHDQMKLDSILKRPYITYQPLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLPNKLRFGGWI
uniprot
proteins
ec_number
5.6.2.2
uniprot
proteins
function
FUNCTION: DNA endonuclease, which specifically mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:39972125, PubMed:39972129, PubMed:39972130). SPO11 covalently attaches itself to the 5' DNA strand during DSB formation and each strand is cleaved by a composite active site assembled at the interface of two SPO11 monomers, with the two cleavages staggered to produce a two-nucleotide 5' overhang (PubMed:39972125, PubMed:39972129, PubMed:39972130). SPO11 cleavage site selection is driven by a mild sequence bias and a preference for bendable and underwound DNA (PubMed:39972125, PubMed:39972129, PubMed:39972130). Together with TOP6BL, promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:26917764). Required for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (PubMed:22346761). {ECO:0000269|PubMed:22346761, ECO:0000269|PubMed:26917764, ECO:0000269|PubMed:39972125, ECO:0000269|PubMed:39972129, ECO:0000269|PubMed:39972130}.; FUNCTION: [Isoform 4]: In contrast to isoform 1, does not mediate DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. {ECO:0000269|PubMed:22346761}.
uniprot
proteins
mass_dalton
44570
uniprot
proteins
mutagenesis
MUTAGEN 137..138; /note="YY->FF: Abolished DNA endonuclease activity."; /evidence="ECO:0000269|PubMed:39972130"; MUTAGEN 137; /note="Y->F: Does not affect DNA endonuclease activity."; /evidence="ECO:0000269|PubMed:39972125"; MUTAGEN 138; /note="Y->F: Abolished DNA endonuclease activity."; /evidence="ECO:0000269|PubMed:39972125, ECO:0000269|PubMed:39972129"; MUTAGEN 224; /note="E->A: Abolished DNA endonuclease activity without affecting ability to bind DNA."; /evidence="ECO:0000269|PubMed:39972129"; MUTAGEN 224; /note="E->Q: Decreased DNA endonuclease activity."; /evidence="ECO:0000269|PubMed:39972125"; MUTAGEN 277; /note="D->N: Strongly decreased DNA endonuclease activity. Knockin mice are unable to form double-strand breaks (DSB) that initiate meiotic recombination."; /evidence="ECO:0000269|PubMed:39972125"; MUTAGEN 279; /note="D->N: Decreased DNA endonuclease activity."; /evidence="ECO:0000269|PubMed:39972125
uniprot
proteins
protein_family
TOP6A family
uniprot
proteins
pubmed
10534401
uniprot
proteins
pubmed
10534402
uniprot
proteins
pubmed
10622720
uniprot
proteins
pubmed
10855504
uniprot
proteins
pubmed
11106738
uniprot
proteins
pubmed
11106739
uniprot
proteins
pubmed
21330546
uniprot
proteins
pubmed
22346761
uniprot
proteins
pubmed
26917764
uniprot
proteins
pubmed
39972125
uniprot
proteins
pubmed
39972129
uniprot
proteins
pubmed
39972130
uniprot
proteins
sequence_length
396
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:11106738, ECO:0000269|PubMed:39972129}. Nucleus {ECO:0000269|PubMed:11106738}. Note=Localizes to discrete foci during leptotene and to homologously synapsed chromosomes. {ECO:0000269|PubMed:11106738}.
uniprot
proteins