Kcnn2
entrez
54262
canonical
uniprot
P70604
canonical
ensembl
ENSRNOG00000016675
resolver
ensp
ENSRNOP00000022530
resolver
genesymbol
Kcnn2
resolver
uniprot
H6VQ94
resolver
uniprot_entry
H6VQ94_RAT
resolver
uniprot_entry
KCNN2_RAT
resolver
activity_regulation
ACTIVITY REGULATION: Inhibited by bee venom neurotoxin apamin (PubMed:20562108, PubMed:8781233). Inhibited by UCL 1684 and tetraethylammonium (TEA) (PubMed:20562108). {ECO:0000269|PubMed:20562108, ECO:0000269|PubMed:8781233}.
uniprot
proteins
amino_acid_sequence
MSSCRYNGGVMRPLSNLSSSRRNLHEMDSEAQPLQPPASVVGGGGGASSPSAAAAASSSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGGGGGSGHGSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQANTLVDLAKTQNIMYDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFIETQMENYDKHVTYNAERSRSSSRRRRSSSTAPPTSSESS
uniprot
proteins
function
FUNCTION: Small conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening (PubMed:11325723, PubMed:20562108, PubMed:21422289, PubMed:8781233, PubMed:9774106). The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 3 picosiemens (PubMed:8781233, PubMed:9774106). Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV (PubMed:8781233, PubMed:9774106). The inward rectification could be due to a blockade of the outward current by intracellular divalent cations such as calcium and magnesium and could also be due to an intrinsic property of the channel pore, independent of intracellular divalent ions (PubMed:11325723, PubMed:21422289). There are three positively charged amino acids in the S6 transmembrane domain, close to the pore, that collectively control the conductance and rectification through an electrostatic mechanism (PubMed:21422289). Additionally, electrostatic contributions from these residues also play an important role in determining the intrinsic open probability of the channel in the absence of calcium, affecting the apparent calcium affinity for activation (PubMed:21422289). Forms an heteromeric complex with calmodulin, which is constitutively associated in a calcium-independent manner (PubMed:9774106). Channel opening is triggered when calcium binds the calmodulin resulting in a rotary movement leading to the formation of the dimeric complex to open the gate (PubMed:11323678, PubMed:9774106). Plays a role in the repolarization phase of cardiac action potential (By similarity). {ECO:0000250|UniProtKB:Q9H2S1, ECO:0000269|PubMed:11323678, ECO:0000269|PubMed:11325723, ECO:0000269|PubMed:20562108, ECO:0000269|PubMed:21422289, ECO:0000269|PubMed:8781233, ECO:0000269|PubMed:9774106}.
uniprot
proteins
mass_dalton
63848
uniprot
proteins
mutagenesis
MUTAGEN 337; /note="H->N: Loss of inhibition by apamin and the organic molecule blockers UCL 1684 and d-tubocurarine. No effect on inhibition by tetraethylammonium (TEA)."; /evidence="ECO:0000269|PubMed:20562108"; MUTAGEN 345; /note="N->G: Reduced inhibition by apamin but binding to apamin is unaffected."; /evidence="ECO:0000269|PubMed:20562108"; MUTAGEN 368; /note="N->H: Reduced inhibition by apamin but binding to apamin is unaffected."; /evidence="ECO:0000269|PubMed:20562108"; MUTAGEN 396; /note="R->E: Mostly eliminates inward rectifier potassium channel activity. Loss of inward rectifier potassium channel activity; when associated with E-397. Significantly increases outward single-channel conductance at positive potentials. Significantly increases outward single-channel conductance at positive potentials; when associated with E-397. Increases inward rectifier potassium channel activity. Does not affect inward rectifier potassium channel activity; when associated with R-399. Decreases inward rectifier potassium channel activity; when associated with E-397 and R-399. Significantly increases apparent calcium affinity. Five-fold increases in the apparent calcium affinity; when associated with E.397. Highly increases apparent calcium affinity; when associated with E-397 and R-399."; /evidence="ECO:0000269|PubMed:21422289"; MUTAGEN 396; /note="R->K: Does not affect inward rectifier potassium channel activity. Does not affect apparent calcium affinity."; /evidence="ECO:0000269|PubMed:21422289"; MUTAGEN 396; /note="R->N: Highly decreases inward rectifier potassium channel activity; when associated with N-397. Slightly decreases apparent calcium affinity."; /evidence="ECO:0000269|PubMed:21422289"; MUTAGEN 397; /note="K->E: Moderately reduces inward rectifier potassium channel activity. Loss of inward rectifier potassium channel activity; when associated with E-396. Significantly increases outward single-channel conductance at positive potentials; when associated with E-396. Decreases inward rectifier potassium channel activity; when associated with E-396 and R-399. Highly increases apparent calcium affinity; when associated with E-396 and R-399."; /evidence="ECO:0000269|PubMed:21422289"; MUTAGEN 397; /note="K->N: Highly decreases inward rectifier potassium channel activity; when associated with N-396."; /evidence="ECO:0000269|PubMed:21422289"; MUTAGEN 399; /note="E->R: Increases inward rectifier potassium channel activity. Does not affect inward rectifier potassium channel activity; when associated with E-396. Decreases inward rectifier potassium channel activity; when associated with E-396 and E-397. Highly increases apparent calcium affinity; when associated with E-396 and E-397."; /evidence="ECO:0000269|PubMed:21422289
uniprot
proteins
protein_family
KCa2.2/KCNN2 subfamily
uniprot
proteins
protein_family
Potassium channel KCNN family
uniprot
proteins
pubmed
11323678
uniprot
proteins
pubmed
11325723
uniprot
proteins
pubmed
16641100
uniprot
proteins
pubmed
20562108
uniprot
proteins
pubmed
21422289
uniprot
proteins
pubmed
8781233
uniprot
proteins
pubmed
9774106
uniprot
proteins
sequence_length
580
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Cytoplasm, myofibril, sarcomere, Z line {ECO:0000250|UniProtKB:P58390}.
uniprot
proteins
transmembrane_region
TRANSMEM 140..160; /note="Helical; Name=Segment S1"; /evidence="ECO:0000255"; TRANSMEM 169..189; /note="Helical; Name=Segment S2"; /evidence="ECO:0000255"; TRANSMEM 215..235; /note="Helical; Name=Segment S3"; /evidence="ECO:0000255"; TRANSMEM 257..277; /note="Helical; Name=Segment S4"; /evidence="ECO:0000255"; TRANSMEM 306..326; /note="Helical; Name=Segment S5"; /evidence="ECO:0000255"; TRANSMEM 375..395; /note="Helical; Name=Segment S6"; /evidence="ECO:0000255
uniprot
proteins