Echs1
entrez
140547
canonical
uniprot
P14604
canonical
ensembl
ENSRNOG00000064647
resolver
ensembl
ENSRNOG00055027621
resolver
ensembl
ENSRNOG00060025390
resolver
ensembl
ENSRNOG00065017885
resolver
ensp
ENSRNOP00000066388
resolver
ensp
ENSRNOP00055039400
resolver
ensp
ENSRNOP00060036505
resolver
ensp
ENSRNOP00065023803
resolver
enst
ENSRNOT00000071574
resolver
enst
ENSRNOT00055047859
resolver
enst
ENSRNOT00060043992
resolver
enst
ENSRNOT00065029959
resolver
genesymbol
Echs1
resolver
uniprot_entry
ECHM_RAT
resolver
amino_acid_sequence
MAALRALLPRACNSLLSPVRCPEFRRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGADIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKANFKDH
uniprot
proteins
ec_number
4.2.1.17
uniprot
proteins
ec_number
5.3.3.8
uniprot
proteins
function
FUNCTION: Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding 3(S)-3-hydroxyacyl-CoA species through addition of a water molecule to the double bond (PubMed:10074351, PubMed:7883013). Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (PubMed:10074351, PubMed:7883013). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (By similarity). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation (PubMed:7883013). At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA) (PubMed:10074351). {ECO:0000250|UniProtKB:P30084, ECO:0000269|PubMed:10074351, ECO:0000269|PubMed:7883013}.
uniprot
proteins
mass_dalton
31516
uniprot
proteins
mutagenesis
MUTAGEN 144; /note="E->D: Reduces activity 50-fold."; /evidence="ECO:0000269|PubMed:12445775"; MUTAGEN 144; /note="E->Q: Reduces activity 3300-fold."; /evidence="ECO:0000269|PubMed:12445775"; MUTAGEN 164; /note="E->D: Reduces activity 1250-fold."; /evidence="ECO:0000269|PubMed:7883013"; MUTAGEN 164; /note="E->Q: Reduces activity 330000-fold."; /evidence="ECO:0000269|PubMed:7883013
uniprot
proteins
pathway_participation
PATHWAY: Lipid metabolism; fatty acid beta-oxidation. {ECO:0000250|UniProtKB:P30084}.
uniprot
proteins
protein_family
Enoyl-CoA hydratase/isomerase family
uniprot
proteins
pubmed
10074351
uniprot
proteins
pubmed
11851409
uniprot
proteins
pubmed
12445775
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
2806264
uniprot
proteins
pubmed
7883013
uniprot
proteins
pubmed
8895557
uniprot
proteins
pubmed
9480773
uniprot
proteins
sequence_length
290
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000269|PubMed:9480773}.
uniprot
proteins