AMOTL2
entrez
51421
canonical
uniprot
Q9Y2J4
canonical
ensembl
ENSG00000114019
resolver
ensp
ENSP00000249883
resolver
ensp
ENSP00000409999
resolver
ensp
ENSP00000424765
resolver
ensp
ENSP00000425475
resolver
genesymbol
AMOTL2
resolver
genesymbol_syn
KIAA0989
resolver
hgnc
HGNC:17812
resolver
uniprot
A8K6F1
resolver
uniprot
B7Z5Q1
resolver
uniprot
E9PHW3
resolver
uniprot
Q53EP1
resolver
uniprot
Q96F99
resolver
uniprot
Q9UKB4
resolver
uniprot_entry
AMOL2_HUMAN
resolver
uniprot_sec
A8K6F1
resolver
uniprot_sec
B7Z5Q1
resolver
uniprot_sec
E9PHW3
resolver
uniprot_sec
Q53EP1
resolver
uniprot_sec
Q96F99
resolver
uniprot_sec
Q9UKB4
resolver
amino_acid_sequence
MRTLEDSSGTVLHRLIQEQLRYGNLTETRTLLAIQQQALRGGAGTGGTGSPQASLEILAPEDSQVLQQATRQEPQGQEHQGGENHLAENTLYRLCPQPSKGEELPTYEEAKAHSQYYAAQQAGTRPHAGDRDPRGAPGGSRRQDEALRELRHGHVRSLSERLLQLSLERNGARAPSHMSSSHSFPQLARNQQGPPLRGPPAEGPESRGPPPQYPHVVLAHETTTAVTDPRYRARGSPHFQHAEVRILQAQVPPVFLQQQQQYQYLQQSQEHPPPPHPAALGHGPLSSLSPPAVEGPVSAQASSATSGSAHLAQMEAVLRENARLQRDNERLQRELESSAEKAGRIEKLESEIQRLSEAHESLTRASSKREALEKTMRNKMDSEMRRLQDFNRDLRERLESANRRLASKTQEAQAGSQDMVAKLLAQSYEQQQEQEKLEREMALLRGAIEDQRRRAELLEQALGNAQGRAARAEEELRKKQAYVEKVERLQQALGQLQAACEKREQLELRLRTRLEQELKALRAQQRQAGAPGGSSGSGGSPELSALRLSEQLREKEEQILALEADMTKWEQKYLEERAMRQFAMDAAATAAAQRDTTLIRHSPQPSPSSSFNEGLLTGGHRHQEMESRLKVLHAQILEKDAVIKVLQQRSRRDPGKAIQGSLRPAKSVPSVFAAAAAGTQGWQGLSSSERQTADAPARLTTDRAPTEEPVVTAPPAAHAKHGSRDGSTQTEGPPDSTSTCLPPEPDSLLGCSSSQRAASLDSVATSRVQDLSDMVEILI
uniprot
proteins
function
FUNCTION: Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Plays a role in coupling actin fibers to cell junctions in endothelial cells and is therefore required for correct endothelial cell morphology via facilitating transcellular transmission of mechanical force resulting in endothelial cell elongation (By similarity). Required for the anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane which facilitates organization of radial actin fiber structure and cellular response to contractile forces (PubMed:28842668). This contributes to maintenance of cell area, size, shape, epithelial sheet organization and trophectoderm cell properties that facilitate blastocyst zona hatching (PubMed:28842668). Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. Activates the Hippo signaling pathway in response to cell contact inhibition via interaction with and ubiquitination by Crumbs complex-bound WWP1 (PubMed:34404733). Ubiquitinated AMOTL2 then interacts with LATS2 which in turn phosphorylates YAP1, excluding it from the nucleus and localizing it to the cytoplasm and tight junctions, therefore ultimately repressing YAP1-driven transcription of target genes (PubMed:17293535, PubMed:21205866, PubMed:26598551). Acts to inhibit WWTR1/TAZ transcriptional coactivator activity via sequestering WWTR1/TAZ in the cytoplasm and at tight junctions (PubMed:23911299). Regulates the size and protein composition of the podosome cortex and core at myofibril neuromuscular junctions (PubMed:23525008). Selectively promotes FGF-induced MAPK activation through SRC (PubMed:17293535). May play a role in the polarity, proliferation and migration of endothelial cells. {ECO:0000250|UniProtKB:Q8K371, ECO:0000269|PubMed:17293535, ECO:0000269|PubMed:21205866, ECO:0000269|PubMed:21937427, ECO:0000269|PubMed:22362771, ECO:0000269|PubMed:23525008, ECO:0000269|PubMed:23911299, ECO:0000269|PubMed:26598551, ECO:0000269|PubMed:28842668, ECO:0000269|PubMed:34404733}.
uniprot
proteins
mass_dalton
85764
uniprot
proteins
mutagenesis
MUTAGEN 107; /note="Y->A: Abolishes interaction with MAGI1 and ACTB. No effect on interaction with CDH1."; /evidence="ECO:0000269|PubMed:28842668"; MUTAGEN 213; /note="Y->A: Abolishes interaction with YAP1 and ubiquitination at K-347 and K-408. Abolishes interaction with WWTR1, but does not affect the interaction between WWTR1 and TTF1 on gene promoter regions. No effect on interaction with CDH1."; /evidence="ECO:0000269|PubMed:23911299, ECO:0000269|PubMed:26598551, ECO:0000269|PubMed:28842668"; MUTAGEN 347; /note="K->R: Abolishes monoubiquitination by WWP1 and reduces interaction with LATS2 and YAP1."; /evidence="ECO:0000269|PubMed:26598551, ECO:0000269|PubMed:34404733"; MUTAGEN 408; /note="K->R: Abolishes monoubiquitination by WWP1 and reduces interaction with LATS2 and YAP1."; /evidence="ECO:0000269|PubMed:26598551, ECO:0000269|PubMed:34404733"; MUTAGEN 776..779; /note="Missing: No effect on interaction with WWTR1."; /evidence="ECO:0000269|PubMed:23911299
uniprot
proteins
post_translational_modification
PTM: Monoubiquitinated at Lys-347 and Lys-408 by Crumbs complex-bound WWP1 (PubMed:34404733). De-ubiquitinated at Lys-347 and Lys-408 by USP9X; the interaction may be promoted by cell contact inhibition (PubMed:26598551, PubMed:34404733). Deubiquitination of AMOTL2 negatively regulates Hippo signaling activation (PubMed:26598551). {ECO:0000269|PubMed:26598551, ECO:0000269|PubMed:34404733}.; PTM: Phosphorylation at Tyr-107 is necessary for efficient binding to SRC and synergistically functioning with SRC to activate the downstream MAPK pathway. {ECO:0000250|UniProtKB:A1YB07}.
uniprot
proteins
protein_family
Angiomotin family
uniprot
proteins
pubmed
10231032
uniprot
proteins
pubmed
12406577
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16641997
uniprot
proteins
pubmed
17293535
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21205866
uniprot
proteins
pubmed
21937427
uniprot
proteins
pubmed
22362771
uniprot
proteins
pubmed
23525008
uniprot
proteins
pubmed
23911299
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
26598551
uniprot
proteins
pubmed
28842668
uniprot
proteins
pubmed
34404733
uniprot
proteins
sequence_length
779
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Recycling endosome {ECO:0000250|UniProtKB:A1YB07}. Cytoplasm {ECO:0000269|PubMed:23911299}. Cell projection, podosome {ECO:0000250|UniProtKB:Q8K371}. Cell junction {ECO:0000269|PubMed:28842668}.
uniprot
proteins