Nemf
entrez
66244
canonical
uniprot
Q8CCP0
canonical
ensembl
ENSMUSG00000020982
resolver
ensp
ENSMUSP00000021368
resolver
ensp
ENSMUSP00000274217
resolver
genesymbol
Nemf
resolver
uniprot
Q3TAS9
resolver
uniprot
Q3UF46
resolver
uniprot
Q66JX6
resolver
uniprot
Q8C9R6
resolver
uniprot
Q8CA65
resolver
uniprot
Q8JZT9
resolver
uniprot
Q8R072
resolver
uniprot
Q9CW30
resolver
uniprot
Q9CYB8
resolver
uniprot
Q9D4A9
resolver
uniprot_entry
NEMF_MOUSE
resolver
uniprot_sec
Q3TAS9
resolver
uniprot_sec
Q3UF46
resolver
uniprot_sec
Q66JX6
resolver
uniprot_sec
Q8C9R6
resolver
uniprot_sec
Q8CA65
resolver
uniprot_sec
Q8JZT9
resolver
uniprot_sec
Q8R072
resolver
uniprot_sec
Q9CW30
resolver
uniprot_sec
Q9CYB8
resolver
uniprot_sec
Q9D4A9
resolver
amino_acid_sequence
MKSRFSTVDLRAVLAELNASLLGMRVNNVYDVDNKTYLIRLQKPDFKATLLLESGIRIHTTEFEWPKNMMPSSFAMKCRKHLKSRRLVSAKQLGVDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDYEYLILNILRFRTDEADDVKFAVRERYPIDHARAAEPLLTLERLTEVIAAAPKGEVLKRVLNPLLPYGPALIEHCLIESGFSGNAKVDEKLESKDIEKILVCVQRAEDYLRKTSNFNGKGYIIQKREAKPSLDADKPAEDILTYEEFHPFLFSQHLQCPYIEFESFDKAVDEFYSKIEGQKIDLKALQQEKQALKKLDNVRKDHENRLEALQQAQEIDKLKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGVIVKEAQAQGDPVACAIKELKLQTNHVTMLLRNPYLLSEEEDGDGDASIENSDAEAPKGKKKKQKNKQLQKPQKNKPLLVDVDLSLSAYANAKKYYDHKRYAAKKTQRTVEAAEKAFKSAEKKTKQTLKEVQTVTSIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEIIVKRYLTPGDIYVHADLHGATSCVIKNPTGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHRGERKVRVQDEDMETLTSCTSELMAEEMEQLEGGDSSEEETEELHGMPGDVELMTQVDQEDIAVHSGRDELSSEDGEAKAVTKDQEPIGEMKEEEEDTFEYPDTTIDLSHLQSQRPLQKLAPREESLNSNDSKSQGRRHLSAKERREMKKKKLPCESGDLEVIEEKDKERESAVHTEAYQNTSKNVAAGQPMKRGQKSKMKKMKEKYKDQDDEDRELIMKLLASAGSNKEEKGKKGKKGKPKDEPVKKPPQKPRGGQRVLDVVKEPPSLQVLAHDLQDLAVDDPHDDKEEHDLDQQGNEENLFDSLTGQPHPEDVLMFAIPICAPYTIMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLHVKRK
uniprot
proteins
function
FUNCTION: Key component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes as well as their ubiquitin-mediated proteasomal degradation (PubMed:33406423). Thereby, frees 60S subunit ribosomes from the stalled translation complex and prevents the accumulation of nascent polypeptide chains that are potentially toxic for the cell (PubMed:33406423). Within the RQC complex, NEMF specifically binds stalled 60S ribosomal subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety and promotes the recruitment of LTN1 to stalled 60S subunits (By similarity). Following binding to stalled 60S ribosomal subunits, NEMF mediates CAT tailing by recruiting alanine-charged tRNA to the A-site and directing the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal alanine extensions (CAT tails) (PubMed:33406423). Mainly recruits alanine-charged tRNAs, but can also other amino acid-charged tRNAs (By similarity). CAT tailing is required to promote ubiquitination of stalled nascent chains by different E3 ubiquitin-protein ligases (By similarity). In the canonical RQC pathway (RQC-L), CAT tailing facilitates LTN1-dependent ubiquitination by exposing lysine residues that would otherwise remain buried in the ribosomal exit tunnel (By similarity). In the alternative RQC pathway (RQC-C) CAT tailing creates an C-degron mainly composed of alanine that is recognized by the CRL2(KLHDC10) and RCHY1/PIRH2 E3 ligases, leading to ubiquitination and degradation of stalled nascent chains (By similarity). NEMF may also indirectly play a role in nuclear export (By similarity). {ECO:0000250|UniProtKB:O60524, ECO:0000250|UniProtKB:Q12532, ECO:0000269|PubMed:33406423}.
uniprot
proteins
mass_dalton
121188
uniprot
proteins
mutagenesis
MUTAGEN 86; /note="R->S: Induces an age-dependent neurodegenerative phenotype."; /evidence="ECO:0000269|PubMed:32934225"; MUTAGEN 106..1064; /note="Missing: Induces an age-dependent neurodegenerative phenotype."; /evidence="ECO:0000269|PubMed:32934225"; MUTAGEN 487; /note="R->G: Induces an age-dependent neurodegenerative phenotype."; /evidence="ECO:0000269|PubMed:32934225
uniprot
proteins
protein_family
NEMF family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17242355
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
32934225
uniprot
proteins
pubmed
33406423
uniprot
proteins
sequence_length
1064
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:O60524}. Nucleus {ECO:0000250|UniProtKB:O60524}.
uniprot
proteins