Stk39
entrez
53416
canonical
uniprot
Q9Z1W9
canonical
ensembl
ENSMUSG00000027030
resolver
ensp
ENSMUSP00000099776
resolver
ensp
ENSMUSP00000189413
resolver
enst
ENSMUST00000102715
resolver
genesymbol
Stk39
resolver
genesymbol_syn
Spak
resolver
uniprot
G1JLS7
resolver
uniprot
Q80W13
resolver
uniprot_entry
STK39_MOUSE
resolver
uniprot_sec
G1JLS7
resolver
uniprot_sec
Q80W13
resolver
activity_regulation
ACTIVITY REGULATION: Activated following phosphorylation at Thr-243 by WNK kinases (WNK1, WNK2, WNK3 or WNK4). {ECO:0000269|PubMed:21486947}.
uniprot
proteins
amino_acid_sequence
MAEPSGSPVHVQLSQQAAPVTAAAATAPAAATSAPAPAPAPAPAASAAPAPAPAAAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAASQEKSRRVKEENSEISVNAGGIPEQIQSLSVHDSQAQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS
uniprot
proteins
ec_number
2.7.11.1
uniprot
proteins
function
FUNCTION: Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed:16382158, PubMed:17488636, PubMed:19633012, PubMed:21486947). Specifically recognizes and binds proteins with a RFXV motif (PubMed:14563843). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed:14563843, PubMed:16382158, PubMed:17488636, PubMed:19633012, PubMed:21486947). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (By similarity). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (By similarity). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed:17488636, PubMed:19633012, PubMed:21486947). Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities (PubMed:21317537, PubMed:23542070). Phosphorylates RELT (PubMed:16530727). {ECO:0000250|UniProtKB:Q9UEW8, ECO:0000269|PubMed:14563843, ECO:0000269|PubMed:16382158, ECO:0000269|PubMed:16530727, ECO:0000269|PubMed:17488636, ECO:0000269|PubMed:19633012, ECO:0000269|PubMed:21317537, ECO:0000269|PubMed:21486947, ECO:0000269|PubMed:23542070}.; FUNCTION: [Isoform 2]: In vitro inhibits isoform 1-catalyzed phosphorylation, such as that of SLC12A1/NKCC2 or SLC12A3/NCC. Also inhibits in vitro OSR1 ability to phosphorylate SLC12A1/NKCC2. {ECO:0000269|PubMed:21907141}.
uniprot
proteins
mass_dalton
60320
uniprot
proteins
mutagenesis
MUTAGEN 104; /note="K->E: Loss of kinase activity but no loss of interaction with RELT."; /evidence="ECO:0000269|PubMed:16530727"; MUTAGEN 243; /note="T->A: Impaired phosphorylation by WNK4 and activation. Abolished ability to activate SLC12A2/NKCC1. Knockin mice are unable to activate SLC12A3/NCC."; /evidence="ECO:0000269|PubMed:16382158, ECO:0000269|PubMed:21486947"; MUTAGEN 243; /note="T->Q: Increased kinase activity and autophosphorylation."; /evidence="ECO:0000269|PubMed:16530727"; MUTAGEN 247; /note="T->A: Abolished ability to activate SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16382158
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Thr-243 by WNK kinases (WNK1, WNK2, WNK3 or WNK4) is required for activation (PubMed:16382158, PubMed:21486947). Autophosphorylation at Thr-243 positively regulates its activity (PubMed:16530727). Phosphorylated at Ser-321 by PRKCQ (PubMed:21733846). {ECO:0000269|PubMed:16382158, ECO:0000269|PubMed:16530727, ECO:0000269|PubMed:21486947, ECO:0000269|PubMed:21733846}.
uniprot
proteins
protein_family
Protein kinase superfamily
uniprot
proteins
protein_family
STE Ser/Thr protein kinase family
uniprot
proteins
protein_family
STE20 subfamily
uniprot
proteins
pubmed
10980603
uniprot
proteins
pubmed
14563843
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16382158
uniprot
proteins
pubmed
16530727
uniprot
proteins
pubmed
17488636
uniprot
proteins
pubmed
19633012
uniprot
proteins
pubmed
20385770
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21317537
uniprot
proteins
pubmed
21486947
uniprot
proteins
pubmed
21733846
uniprot
proteins
pubmed
21907141
uniprot
proteins
pubmed
23542070
uniprot
proteins
sequence_length
556
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}. Apical cell membrane {ECO:0000269|PubMed:14563843}. Note=Nucleus when caspase-cleaved. In choroid plexus cells, found at the apical plasma membrane of epithelial cells (PubMed:14563843). {ECO:0000269|PubMed:14563843, ECO:0000305}.
uniprot
proteins