Chrna4
entrez
25590
canonical
uniprot
P09483
canonical
ensembl
ENSRNOG00000011202
resolver
ensp
ENSRNOP00000073967
resolver
genesymbol
Chrna4
resolver
genesymbol_syn
Acra4
resolver
uniprot
O35769
resolver
uniprot_entry
ACHA4_RAT
resolver
uniprot_sec
O35769
resolver
activity_regulation
ACTIVITY REGULATION: Activated by a myriad of ligands such as acetylcholine, cytisine, nicotine, choline and epibatidine (PubMed:22593584, PubMed:9882698). Channel potentiation by calcium is stoichiometry-selective, CHRNA4:CHRNB2 nACh receptor is achieved by calcium association with topographically distinct sites framed by anionic residues within the CHRNA4 subunit and between the CHRNA4 and CHRNB2 subunits (By similarity). nAChR activity is inhibited by the antagonist alpha-conotoxins BuIA, PnIA, GID and MII, small disulfide-constrained peptides from cone snails (PubMed:15929983, PubMed:22751014). {ECO:0000250|UniProtKB:P43681, ECO:0000269|PubMed:15929983, ECO:0000269|PubMed:22593584, ECO:0000269|PubMed:22751014, ECO:0000269|PubMed:9882698}.
uniprot
proteins
amino_acid_sequence
MANSGTGAPPPLLLLPLLLLLGTGLLPASSHIETRAHAEERLLKRLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLCISVLLSLTVFLLLITEIIPSPTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVFLDIVPRLLFMKRPSVVKDNCRRLIESMHKMANAPRFWPEPVGEPGILSDICNQGLSPAPTFCNPTDTAVETQPTCRSPPLEVPDLKTSEVEKASPCPSPGSCPPPKSSSGAPMLIKARSLSVQHVPSSQEAAEDGIRCRSRSIQYCVSQDGAASLADSKPTSSPTSLKARPSQLPVSDQASPCKCTCKEPSPVSPVTVLKAGGTKAPPQHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAAC
uniprot
proteins
function
FUNCTION: Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (Probable). CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits. Is the most abundant nAChR subtype expressed in the central nervous system (By similarity). Found in two major stoichiometric forms,(CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (By similarity). Involved in the modulation of calcium-dependent signaling pathways, influences the release of neurotransmitters, including dopamine, glutamate and GABA (By similarity). {ECO:0000250|UniProtKB:O70174, ECO:0000250|UniProtKB:P43681, ECO:0000305|PubMed:9882698}.
uniprot
proteins
mass_dalton
70193
uniprot
proteins
mutagenesis
MUTAGEN 219..240; /note="TYNTRKYECCAEIYPDITYAFI->YKHDIKYNCCEEIYTDITYSFY: >10000-fold increase in inhibition of CHRNA4:CHRNB2 nAChR by the conotoxin BuIA."; /evidence="ECO:0000269|PubMed:22751014"; MUTAGEN 220..223; /note="YNTR->KHDI: >1700-fold increase in inhibition of CHRNA4:CHRNB2 nAChR by the conotoxin BuIA."; /evidence="ECO:0000269|PubMed:22751014"; MUTAGEN 223; /note="R->I: >10-fold increase in inhibition of CHRNA4:CHRNB2 nAChR by the conotoxin BuIA."; /evidence="ECO:0000269|PubMed:22751014"; MUTAGEN 273; /note="C->S: Loss of palmitoylation. Decreases surface expression but presynaptic terminals targeting. No effect on ligand binding."; /evidence="ECO:0000269|PubMed:22593584
uniprot
proteins
protein_family
Acetylcholine receptor (TC 1.A.9.1) subfamily
uniprot
proteins
protein_family
Alpha-4/CHRNA4 sub-subfamily
uniprot
proteins
protein_family
Ligand-gated ion channel (TC 1.A.9) family
uniprot
proteins
pubmed
15929983
uniprot
proteins
pubmed
22593584
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
22751014
uniprot
proteins
pubmed
3609304
uniprot
proteins
pubmed
3829125
uniprot
proteins
pubmed
9882698
uniprot
proteins
sequence_length
630
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Presynaptic cell membrane {ECO:0000269|PubMed:22593584}; Multi-pass membrane protein {ECO:0000269|PubMed:22593584}. Cell membrane {ECO:0000250|UniProtKB:O70174}; Multi-pass membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 250..270; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 279..299; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 313..333; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 605..625; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins