Rad50
entrez
64012
canonical
uniprot
Q9JIL8
canonical
ensembl
ENSRNOG00000033065
resolver
ensp
ENSRNOP00000062378
resolver
enst
ENSRNOT00000063772
resolver
genesymbol
Rad50
resolver
uniprot
G3V9X6
resolver
uniprot_entry
G3V9X6_RAT
resolver
uniprot_entry
RAD50_RAT
resolver
amino_acid_sequence
MSRIEKMSTLGVRSFGIEDKDKQIISFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGEMVLVQRSMLCSQKSKKTEFKTLEGVITRIKHGEKVSLSSKCAEIDREMISCLGVSKSVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALDTLRQVRQTQGQKVKECQTELKYLRQNKEKACEIRDQITSKEAQLASSREIVKAYENELEPLKNRLKEIEHNLSKIMRLDNEIKALDSRKKQMEKDNSELEQKMEKVFQGTDEQLNDLYHNHQRTVREKERRLVDCQRELEKLSKEARLLNQERAELLVEQGRLQLQADRHQEHIRARDSLIQSLAAHLELDGFERGPFSERQIKNFHELVRERQEREAKTASQLLSDLTDKEALKQRQMDEMRDKKSGLGRMIELKTEILTKKQTELRNVRNELQQLEGSSDRILELDQELTKAERELSKAEKNSSIETLKAEILNLQSEKADLDRNLRKLDQEMEQLNHHTTTRTQMEMLTKDKTDKDEQIRKIKSRHSDELTSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASAEQNKNHINNELKKKEEQLSSYEDKLFDVCGSQDFESDLDRLKEDIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPGCQRVFQTEAELQEVISDLQSKLRLAPDKLKSTESELKKKERRRDEMLGLVPMRQSIIDLKEKEIPELRNRLQSVNRDIQRLKNDIEEQETLLGTVMPEEESAKVCLTDVTIMERFQMELKDVERKIAQQAAKLQGVDLDRTVQQVNQEKQEKQHKLDTVSSKIELNRKLIQDQQEQIQHLKSKTNELKSEKLQIATNLQRRQQMEEQTVELSTEVQSLNREIKDAKEQINPLEIALEKLQQEKEELIHRKNTSNKMAQDKINDIKEKVKNIHGYMKDIENYIQDGKDDYKKQKETELNEVVIQLNECDKHKEKINKEMGTMRQDIDTKKIQERWLQDNLTLRKRREELKEVEEERKQHLKEMGQMQVLQMKNEHQKLEENIDTIKRNHSLALGRQKGYEEEILHFKKELREPQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDHAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVVMLKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRVKKNIDQCSEIVKSSINSLGSYVH
uniprot
proteins
ec_number
3.6.-.-
uniprot
proteins
function
FUNCTION: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the complex, RAD50 is both required to bind DNA ends and hold them in close proximity and regulate the activity of MRE11 (By similarity). RAD50 provides an ATP-dependent control of MRE11 by positioning DNA ends into the MRE11 active site: ATP-binding induces a large structural change from an open form with accessible MRE11 nuclease sites into a closed form (By similarity). The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR. The MRN complex is also required for the processing of R-loops. In telomeres the MRN complex may modulate t-loop formation (By similarity). {ECO:0000250|UniProtKB:Q92878, ECO:0000250|UniProtKB:Q9X1X1}.
uniprot
proteins
mass_dalton
153784
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Ser-635 by ATM in response to DNA damage is required for double-strand break (DSB) repair. {ECO:0000250|UniProtKB:Q92878}.
uniprot
proteins
protein_family
RAD50 subfamily
uniprot
proteins
protein_family
SMC family
uniprot
proteins
pubmed
10908350
uniprot
proteins
sequence_length
1312
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q92878}. Chromosome, telomere {ECO:0000250|UniProtKB:Q92878}. Chromosome {ECO:0000250|UniProtKB:Q92878}. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. Localizes to DNA double-strand breaks (DSBs). {ECO:0000250|UniProtKB:Q92878}.
uniprot
proteins