Dxo
entrez
112403
canonical
uniprot
O70348
canonical
ensembl
ENSMUSG00000040482
resolver
ensp
ENSMUSP00000047018
resolver
ensp
ENSMUSP00000137234
resolver
ensp
ENSMUSP00000202999
resolver
enst
ENSMUST00000046244
resolver
enst
ENSMUST00000180043
resolver
genesymbol
Dxo
resolver
genesymbol_syn
Dom3z
resolver
genesymbol_syn
Ng6
resolver
uniprot
A0A0R4J288
resolver
uniprot
Q99KD8
resolver
uniprot_entry
A0A0R4J288_MOUSE
resolver
uniprot_entry
DXO_MOUSE
resolver
uniprot_sec
Q99KD8
resolver
activity_regulation
ACTIVITY REGULATION: The 5'-3' exoribonuclease activity is inhibited by adenosine 3',5'-bisphosphate. {ECO:0000269|PubMed:30180947}.
uniprot
proteins
amino_acid_sequence
MEPRGTKRKAEKTEVEKPLNKLPRAVPSLRTQPSLYSGPFPFYRRPSELGCFSLDAQRQYHGDARALRYYSPPPINGPGPDFDLRDGYPDRYQPRDEEVQERLDHLLRWVLEHRNQLEGGPGWLAGATVTWRGHLTKLLTTPYERQEGWQLAASRFQGTLYLSEVETPAARAQRLARPPLLRELMYMGYKFEQYMCADKPGGSPDPSGEVNTNVAYCSVLRSRLGNHPLLFSGEVDCLNPQAPCTQPPSCYVELKTSKEMHSPGQWRSFYRHKLLKWWAQSFLPGVPHVVAGFRNPEGFVCSLKTFPTMEMFENVRNDREGWNPSVCMNFCAAFLSFAQSTVVQDDPRLVHLFSWEPGGPVTVSVHRDAPYAFLPSWYVETMTQDLPPLSKTPSPKD
uniprot
proteins
ec_number
3.1.13.-
uniprot
proteins
ec_number
3.6.1.-
uniprot
proteins
function
FUNCTION: Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA (PubMed:28283058, PubMed:32374864). The NAD-cap is present at the 5'-end of some RNAs and snoRNAs (PubMed:28283058). In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (PubMed:28283058). Preferentially acts on NAD-capped transcripts in response to environmental stress (By similarity). Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation (PubMed:23523372, PubMed:28283058). Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control (PubMed:23523372). Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1) (PubMed:23523372). In contrast to canonical decapping enzymes DCP2 and NUDT16, which cleave the cap within the triphosphate linkage, the decapping activity releases the entire cap structure GpppN and a 5'-end monophosphate RNA (PubMed:23523372). Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs (PubMed:23523372, PubMed:30180947). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates (PubMed:23523372). Exhibits decapping activity towards FAD-capped RNAs (PubMed:32374864, PubMed:32432673). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (PubMed:32374864, PubMed:32432673). {ECO:0000250|UniProtKB:O77932, ECO:0000269|PubMed:23523372, ECO:0000269|PubMed:28283058, ECO:0000269|PubMed:30180947, ECO:0000269|PubMed:32374864, ECO:0000269|PubMed:32432673}.
uniprot
proteins
mass_dalton
45280
uniprot
proteins
mutagenesis
MUTAGEN 234; /note="E->A: Abolishes the decapping activity on both incomplete m7G cap and NAD-cap RNAs. Abolishes the 5'-3' exoribonuclease activity."; /evidence="ECO:0000269|PubMed:23523372, ECO:0000269|PubMed:28283058, ECO:0000269|PubMed:30180947"; MUTAGEN 236; /note="D->A: Abolishes the decapping activity on both incomplete m7G cap and NAD-cap RNAs."; /evidence="ECO:0000269|PubMed:23523372, ECO:0000269|PubMed:28283058
uniprot
proteins
protein_family
DXO/Dom3Z family
uniprot
proteins
pubmed
14656967
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
19194460
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
23523372
uniprot
proteins
pubmed
28283058
uniprot
proteins
pubmed
30180947
uniprot
proteins
pubmed
32374864
uniprot
proteins
pubmed
32432673
uniprot
proteins
sequence_length
397
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O77932}.
uniprot
proteins