Prim1
entrez
19075
canonical
uniprot
P20664
canonical
ensembl
ENSMUSG00000025395
resolver
ensp
ENSMUSP00000026461
resolver
enst
ENSMUST00000026461
resolver
genesymbol
Prim1
resolver
uniprot
Q5FW94
resolver
uniprot_entry
PRI1_MOUSE
resolver
uniprot_entry
Q5FW94_MOUSE
resolver
activity_regulation
ACTIVITY REGULATION: The presence of the regulatory subunit PRIM2/p58 accelerates the kinetics of initiation and primer extension. {ECO:0000269|PubMed:8253737}.
uniprot
proteins
amino_acid_sequence
MEPFDPAELPELLKLYYRRLFPYAQYYRWLNYGGVTKNYFQHREFSFTLKDDIYIRYQSFNNQSELEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLNEKVHPFVRKSINIIKKYFEEYALVGQDILENKENWDKILALVPETIHDELQRGFQKFHSSPQRWEHLRKVANSSQNMKNDKCGPWLEWEVMLQYCFPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDFHKVDQFDPFTVPTISAICRELDMVSTHEKEKEENEADSKHRVRGYKKTSLAPYVKVFEQFLENLDKSRKGELLKKSDLQKDF
uniprot
proteins
ec_number
2.7.7.102
uniprot
proteins
function
FUNCTION: Catalytic subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis (PubMed:8026492, PubMed:8253737). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerizing short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity). In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit (PubMed:8253737). Can add both ribo- and deoxynucleotides during elongation of the primers (PubMed:8253737). Synthesizes 9-mer RNA primers (also known as the 'unit length' RNA primers) (By similarity). Incorporates only ribonucleotides in the presence of ribo- and deoxy-nucleotide triphosphates (rNTPs, dNTPs). Requires template thymine or cytidine to start the RNA primer synthesis, with an adenine or guanine at its 5'-end (By similarity). Binds single stranded DNA (PubMed:8253737). {ECO:0000250|UniProtKB:P09884, ECO:0000250|UniProtKB:P49642, ECO:0000269|PubMed:8026492, ECO:0000269|PubMed:8253737}.
uniprot
proteins
mass_dalton
49295
uniprot
proteins
protein_family
Eukaryotic-type primase small subunit family
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
2925677
uniprot
proteins
pubmed
8026492
uniprot
proteins
pubmed
8253737
uniprot
proteins
pubmed
8463324
uniprot
proteins
sequence_length
417
uniprot
proteins