Otub1
entrez
293705
canonical
uniprot
B2RYG6
canonical
ensembl
ENSRNOG00000021175
resolver
ensembl
ENSRNOG00055022962
resolver
ensembl
ENSRNOG00060032941
resolver
ensembl
ENSRNOG00065030799
resolver
ensp
ENSRNOP00000028752
resolver
ensp
ENSRNOP00055032041
resolver
ensp
ENSRNOP00060047202
resolver
ensp
ENSRNOP00065043617
resolver
enst
ENSRNOT00000028752
resolver
enst
ENSRNOT00055039466
resolver
enst
ENSRNOT00060057122
resolver
enst
ENSRNOT00065053099
resolver
genesymbol
Otub1
resolver
uniprot_entry
OTUB1_RAT
resolver
activity_regulation
ACTIVITY REGULATION: By free ubiquitin: binding of free ubiquitin triggers conformational changes in the OTU domain and formation of a ubiquitin-binding helix in the N-terminus, promoting binding of the conjugated donor ubiquitin in UBE2N/UBC13 to OTUB1. {ECO:0000250|UniProtKB:Q96FW1}.
uniprot
proteins
amino_acid_sequence
MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHVFPEGSEPKVYLLYRPGHYDILYK
uniprot
proteins
ec_number
3.4.19.12
uniprot
proteins
function
FUNCTION: Hydrolase that can specifically remove compared to 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation (By similarity). Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen (By similarity). Acts via its interaction with RNF128/GRAIL (By similarity). Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128 (By similarity). Deubiquitinates estrogen receptor alpha (ESR1) (By similarity). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains (By similarity). Not able to cleave di-ubiquitin (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin (By similarity). {ECO:0000250|UniProtKB:Q96FW1}.; FUNCTION: Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain. Acts as a regulator of mTORC1 and mTORC2 complexes. When phosphorylated at Tyr-26, acts as an activator of the mTORC1 complex by mediating deubiquitination of RPTOR via a non-catalytic process: acts by binding and inhibiting the activity of the ubiquitin-conjugating enzyme E2 (UBE2D1/UBCH5A, UBE2W/UBC16 and UBE2N/UBC13), thereby preventing ubiquitination of RPTOR. Can also act as an inhibitor of the mTORC1 and mTORC2 complexes in response to amino acids by mediating non-catalytic deubiquitination of DEPTOR. {ECO:0000250|UniProtKB:Q96FW1}.
uniprot
proteins
mass_dalton
31270
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Tyr-26 by SRC and SRMS promotes deubiquitination of RPTOR via a non-catalytic process. {ECO:0000250|UniProtKB:Q96FW1}.
uniprot
proteins
protein_family
Peptidase C65 family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
19343716
uniprot
proteins
sequence_length
271
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19343716}.
uniprot
proteins