Kcnk1
entrez
16525
canonical
uniprot
O08581
canonical
ensembl
ENSMUSG00000033998
resolver
ensp
ENSMUSP00000046103
resolver
ensp
ENSMUSP00000148778
resolver
enst
ENSMUST00000046765
resolver
enst
ENSMUST00000212831
resolver
genesymbol
Kcnk1
resolver
uniprot
A0A1D5RMH2
resolver
uniprot
Q99L99
resolver
uniprot_entry
A0A1D5RMH2_MOUSE
resolver
uniprot_entry
KCNK1_MOUSE
resolver
uniprot_sec
Q99L99
resolver
activity_regulation
ACTIVITY REGULATION: Inhibited by quinine, quinidine, barium, and internal acidification. {ECO:0000269|PubMed:9013852, ECO:0000305|PubMed:16847696}.
uniprot
proteins
amino_acid_sequence
MLQSLAGSSCVRLVERHRSAWCFGFLVLGYLLYLVFGAVVFSSVELPYEDLLRQELRKLKRRFLEEHECLSEPQLEQFLGRVLEASNYGVSVLSNASGNWNWDFTSALFFASTVLSTTGYGHTVPLSDGGKAFCIIYSVIGIPFTLLFLTAVVQRVTVHVTRRPVLYFHIRWGFSKQVVAIVHAVLLGFVTVSCFFFIPAAVFSVLEDDWNFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMFYVKKDKDEDLVHIMEHDQLSFSSVTEQVAGLKEEQKQSEPFVASQSPPYEDGSADH
uniprot
proteins
function
FUNCTION: Ion channel that contributes to passive transmembrane potassium transport and to the regulation of the resting membrane potential in brain astrocytes, but also in kidney and in other tissues (PubMed:16847696, PubMed:22431633, PubMed:24368895). Forms dimeric channels through which potassium ions pass in accordance with their electrochemical gradient (By similarity). The channel is selective for K(+) ions at physiological potassium concentrations and at neutral pH, but becomes permeable to Na(+) at subphysiological K(+) levels and upon acidification of the extracellular medium (By similarity). The homodimer has very low potassium channel activity, when expressed in heterologous systems, and can function as weakly inward rectifying potassium channel (PubMed:24496152, PubMed:9013852). Channel activity is modulated by activation of serotonin receptors (PubMed:24368895). Heterodimeric channels containing KCNK1 and KCNK2 have much higher activity, and may represent the predominant form in astrocytes (PubMed:24496152). Heterodimeric channels containing KCNK1 and KCNK3 or KCNK9 have much higher activity (By similarity). Heterodimeric channels formed by KCNK1 and KCNK9 may contribute to halothane-sensitive currents (By similarity). Mediates outward rectifying potassium currents in dentate gyrus granule cells and contributes to the regulation of their resting membrane potential (PubMed:25406588). Contributes to the regulation of action potential firing in dentate gyrus granule cells and down-regulates their intrinsic excitability (PubMed:25406588). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G protein-coupled receptors, such as F2R and CNR1 (PubMed:24496152). Required for normal ion and water transport in the kidney (PubMed:16025300). Contributes to the regulation of the resting membrane potential of pancreatic beta cells (PubMed:22431633). The low channel activity of homodimeric KCNK1 may be due to sumoylation (By similarity). The low channel activity may be due to rapid internalization from the cell membrane and retention in recycling endosomes (PubMed:15540117). Permeable to monovalent cations with ion selectivity for K(+) > Rb(+) >> NH4(+) >> Cs(+) = Na(+) = Li(+). {ECO:0000250|UniProtKB:O00180, ECO:0000250|UniProtKB:Q9Z2T2, ECO:0000269|PubMed:15540117, ECO:0000269|PubMed:16025300, ECO:0000269|PubMed:16847696, ECO:0000269|PubMed:22431633, ECO:0000269|PubMed:24368895, ECO:0000269|PubMed:24496152, ECO:0000269|PubMed:9013852}.
uniprot
proteins
mass_dalton
38201
uniprot
proteins
mutagenesis
MUTAGEN 69; /note="C->S: Abolishes formation of a disulfide-linked heterodimer with KCNK2."; /evidence="ECO:0000269|PubMed:24496152"; MUTAGEN 119; /note="G->E: Abolishes potassium channel activity."; /evidence="ECO:0000269|PubMed:24496152"; MUTAGEN 293..294; /note="IM->AA: Increases channel expression at the cell membrane, resulting in higher channel activity."; /evidence="ECO:0000269|PubMed:19959478
uniprot
proteins
post_translational_modification
PTM: Sumoylation is controversial. Sumoylated by UBE2I. Not sumoylated when expressed in xenopus oocytes or mammalian cells. Sumoylation inactivates the channel, but does not interfere with expression at the cell membrane. Sumoylation of a single subunit is sufficient to silence the dimeric channel. Sumoylation of KCNK1 is sufficient to silence heterodimeric channels formed by KCNK1 and KCNK3 or KCNK9. Desumoylated by SENP1; this activates the channel. Desumoylated by SENP1; this strongly increases halothane-mediated activation of heterodimeric channels formed with KCNK9. SENP1 treatment has no effect. {ECO:0000250|UniProtKB:O00180}.
uniprot
proteins
protein_family
Two pore domain potassium channel (TC 1.A.1.8) family
uniprot
proteins
pubmed
12855359
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15540117
uniprot
proteins
pubmed
16025300
uniprot
proteins
pubmed
16847696
uniprot
proteins
pubmed
17079103
uniprot
proteins
pubmed
18222039
uniprot
proteins
pubmed
19959478
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22431633
uniprot
proteins
pubmed
24368895
uniprot
proteins
pubmed
24496152
uniprot
proteins
pubmed
25339226
uniprot
proteins
pubmed
25406588
uniprot
proteins
pubmed
25530075
uniprot
proteins
pubmed
9013852
uniprot
proteins
pubmed
9559671
uniprot
proteins
sequence_length
336
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15540117, ECO:0000269|PubMed:19959478, ECO:0000269|PubMed:24368895, ECO:0000269|PubMed:24496152, ECO:0000269|PubMed:9013852}; Multi-pass membrane protein {ECO:0000305}. Recycling endosome {ECO:0000269|PubMed:15540117}. Apical cell membrane {ECO:0000269|PubMed:12855359, ECO:0000305|PubMed:16025300}. Cytoplasmic vesicle {ECO:0000269|PubMed:16025300, ECO:0000269|PubMed:24368895}. Perikaryon {ECO:0000269|PubMed:12855359, ECO:0000269|PubMed:17079103, ECO:0000269|PubMed:25406588}. Cell projection, dendrite {ECO:0000269|PubMed:25406588}. Cell projection {ECO:0000269|PubMed:17079103}. Synaptic cell membrane {ECO:0000250|UniProtKB:Q9Z2T2}. Note=The heterodimer with KCNK2 is detected at the astrocyte cell membrane (PubMed:24496152). Not detected at the astrocyte cell membrane when KCNK2 is absent (PubMed:24496152). Detected on neuronal cell bodies, and to a lesser degree on neuronal cell projections (PubMed:12855359, PubMed:17079103). Detected on hippocampus dentate gyrus granule cell bodies and to a lesser degree on proximal dendrites (PubMed:25406588). Detected at the apical cell membrane in stria vascularis in the cochlea (By similarity). Detected at the apical cell membrane of vestibular dark cells situated between the crista and the utricle in the inner ear (PubMed:12855359). Detected at the apical cell membrane in stria vascularis in the cochlea (PubMed:12855359). Detected at the apical cell membrane in kidney proximal tubule segment S1 and in subapical compartments in segments S1, S2 and S3 (PubMed:16025300). Predominantly in cytoplasmic structures in kidney distal convoluted tubules and collecting ducts (PubMed:16025300). Predominantly in cytoplasmic structures in hippocampus astrocytes; only a minor proportion of the protein is present at the cell membrane (PubMed:24368895). {ECO:0000250|UniProtKB:Q9Z2T2, ECO:0000269|PubMed:16025300, ECO:0000269|PubMed:17079103, ECO:0000269|PubMed:24368895, ECO:0000269|PubMed:24496152, ECO:0000269|PubMed:25406588}.
uniprot
proteins
transmembrane_region
TRANSMEM 21..41; /note="Helical; Name=M1"; /evidence="ECO:0000250|UniProtKB:O00180"; TRANSMEM 133..156; /note="Helical; Name=M2"; /evidence="ECO:0000250|UniProtKB:O00180"; TRANSMEM 182..202; /note="Helical; Name=M3"; /evidence="ECO:0000250|UniProtKB:O00180"; TRANSMEM 244..267; /note="Helical; Name=M4"; /evidence="ECO:0000250|UniProtKB:O00180
uniprot
proteins