Pex5
entrez
312703
canonical
uniprot
Q2M2R8
canonical
ensembl
ENSRNOG00000049203
resolver
ensp
ENSRNOP00000014028
resolver
ensp
ENSRNOP00000064300
resolver
enst
ENSRNOT00000014028
resolver
enst
ENSRNOT00000071625
resolver
genesymbol
Pex5
resolver
genesymbol_syn
Pex5_predicted
resolver
uniprot
A0A9M1WVX5
resolver
uniprot
M0R4L3
resolver
uniprot_entry
A0A9M1WVX5_RAT
resolver
uniprot_entry
M0R4L3_RAT
resolver
uniprot_entry
PEX5_RAT
resolver
activity_regulation
ACTIVITY REGULATION: Cys-11 acts as a sensor of redox state. In response to oxidative stress, monoubiquitination at Cys-11 is prevented. {ECO:0000250|UniProtKB:P50542}.
uniprot
proteins
amino_acid_sequence
MAMRELVESECGGANPLMKLATHFTQDKALRQEGLRPPWPPGASAAETVSKPLGVGTEDELVAEFLQDQNATLVSRAPQTFKMDDLLAEMQEIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVAQDYNETDWSQEFIAEVTDPLSVSPARWAEEYLEQSEEKLWLGDPEGTSTTDRWYDEYHPEEDLQHTASDFVSKVDDPKLANSEFLKFVRQIGEGQVSLESAAGSGRAQAEQWAAEFIQQQGTSEAWVDQFTRPGNKLAALQVEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSASYDKGYQFEEENPLRDHPQPFEEGLRRLEEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNRTALMALAVSFTNESLQRQACETLRDWLRYSPAYAHLVAPGEEGASGAGLGPSKRVLGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLPALLTMFGLPQ
uniprot
proteins
function
FUNCTION: Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle. {ECO:0000250|UniProtKB:P50542}.; FUNCTION: In addition to promoting peroxisomal translocation of proteins containing a PTS1 peroxisomal targeting signal, mediates peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal via its interaction with PEX7. Interaction with PEX7 only takes place when PEX7 is associated with cargo proteins containing a PTS2 peroxisomal targeting signal. PEX7 along with PTS2-containing cargo proteins are then translocated through the PEX13-PEX14 docking complex together with PEX5. {ECO:0000250|UniProtKB:P50542}.
uniprot
proteins
mass_dalton
71022
uniprot
proteins
post_translational_modification
PTM: Monoubiquitinated at Cys-11 by PEX2 during PEX5 passage through the retrotranslocation channel (By similarity). Cys-11 monoubiquitination acts as a recognition signal for the PEX1-PEX6 complex and is required for PEX5 extraction and export from peroxisomes. Monoubiquitination at Cys-11 is removed by USP9X in the cytosol, resetting PEX5 for a subsequent import cycle (By similarity). When PEX5 recycling is compromised, polyubiquitinated by PEX10 during its passage through the retrotranslocation channel, leading to its degradation (By similarity). Monoubiquitination at Lys-473 by TRIM37 promotes its stability by preventing its polyubiquitination and degradation by the proteasome. Ubiquitination at Lys-528 is not mediated by the PEX2-PEX10-PEX12 ligase complex and is not related to PEX5 recycling. Monoubiquitinated at Lys-209 by PEX2 following phosphorylation by ATM in response to starvation or reactive oxygen species (ROS), leading to PEX5 recognition by p62/SQSTM1 and induction of pexophagy (By similarity). {ECO:0000250|UniProtKB:P35056, ECO:0000250|UniProtKB:P50542}.; PTM: Phosphorylated at Ser-140 by ATM in response to reactive oxygen species (ROS), promoting monoubiquitination at Lys-209 and induction of pexophagy. {ECO:0000250|UniProtKB:P50542}.
uniprot
proteins
protein_family
Peroxisomal targeting signal receptor family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
18359941
uniprot
proteins
sequence_length
640
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P50542}. Peroxisome matrix {ECO:0000250|UniProtKB:P50542}. Note=Cycles between the cytosol and the peroxisome matrix (By similarity). Following binding to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, recruited to the docking complex, composed of PEX13 and PEX14, leading to translocation into the peroxisome matrix along with cargo proteins. Export and recycling to the cytosol is initiated by binding to the PEX2-PEX10-PEX12 ligase complex via its unstructured N-terminus that inserts into the ligase pore and emerges in the cytosol (By similarity). Cys-11 of PEX5 is then monoubiquitinated, promoting its extraction from peroxisomal membrane by the PEX1-PEX6 AAA ATPase complex (By similarity). Extraction is accompanied by unfolding of the TPR repeats and release of bound cargo in the peroxisome matrix. The TPR repeats refold in the cytosol and ubiquitination is removed by deubiquitinating enzymes, resetting PEX5 for a subsequent import cycle (By similarity). {ECO:0000250|UniProtKB:A0A1L8FDW4, ECO:0000250|UniProtKB:P50542}.
uniprot
proteins