Slc25a31
entrez
73333
canonical
uniprot
Q3V132
canonical
ensembl
ENSMUSG00000069041
resolver
ensp
ENSMUSP00000088723
resolver
enst
ENSMUST00000091184
resolver
genesymbol
Slc25a31
resolver
genesymbol_syn
Aac4
resolver
genesymbol_syn
Ant4
resolver
genesymbol_syn
Sfec
resolver
uniprot
B2RTC8
resolver
uniprot
Q80W28
resolver
uniprot_entry
ADT4_MOUSE
resolver
uniprot_entry
B2RTC8_MOUSE
resolver
uniprot_sec
Q80W28
resolver
activity_regulation
ACTIVITY REGULATION: The matrix-open state (m-state) is inhibited by the membrane-permeable bongkrekic acid (BKA). The cytoplasmic-open state (c-state) is inhibited by the membrane-impermeable toxic inhibitor carboxyatractyloside (CATR) (By similarity). Proton transporter activity is inhibited by ADP:ATP antiporter activity (By similarity). {ECO:0000250|UniProtKB:G2QNH0, ECO:0000250|UniProtKB:P48962}.
uniprot
proteins
amino_acid_sequence
MSNESSKKQSSKKALFDPVSFSKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMLDCLVRIPREQGFLSYWRGNLANVIRYFPTQALNFAFKDKYKELFMSGVNKEKQFWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEQRQFTGLGDCIMKIAKSDGLIGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKETPFLVSFIIAQIVTTCSGILSYPFDTVRRRMMMQSGESDRQYKGTIDCFLKIYRHEGVPAFFRGAFSNILRGTGGALVLVLYDKIKEFLNIDVGGSSSGD
uniprot
proteins
function
FUNCTION: ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (By similarity). Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity). Specifically required during spermatogenesis, probably to mediate ADP:ATP exchange in spermatocytes (PubMed:17137571, PubMed:17681941, PubMed:19556438). Large ATP supplies from mitochondria may be critical for normal progression of spermatogenesis during early stages of meiotic prophase I, including DNA double-strand break repair and chromosomal synapsis (PubMed:19556438). In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (PubMed:31489369). Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (By similarity). Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A31/ANT4 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it (By similarity). Among nucleotides, may also exchange ADP for dATP and dADP (By similarity). Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (PubMed:31489369). It is however unclear if SLC25A31/ANT4 constitutes a pore-forming component of mPTP or regulates it (PubMed:31489369). {ECO:0000250|UniProtKB:G2QNH0, ECO:0000250|UniProtKB:P48962, ECO:0000250|UniProtKB:Q9H0C2, ECO:0000269|PubMed:17137571, ECO:0000269|PubMed:17681941, ECO:0000269|PubMed:19556438, ECO:0000269|PubMed:31489369}.
uniprot
proteins
mass_dalton
35258
uniprot
proteins
protein_family
Mitochondrial carrier (TC 2.A.29) family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16051982
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17137571
uniprot
proteins
pubmed
17681941
uniprot
proteins
pubmed
18667754
uniprot
proteins
pubmed
19556438
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
31489369
uniprot
proteins
sequence_length
320
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250|UniProtKB:P02722, ECO:0000250|UniProtKB:Q9H0C2}; Multi-pass membrane protein {ECO:0000255}. Membrane {ECO:0000250|UniProtKB:Q9H0C2}; Multi-pass membrane protein {ECO:0000255}. Cell projection, cilium, flagellum membrane {ECO:0000269|PubMed:17137571}; Multi-pass membrane protein {ECO:0000255}. Note=In sperm flagellum this protein is located in the fibrous sheath, a non-mitochondrial region (By similarity). May localize to non-mitochondrial membranes (By similarity). {ECO:0000250|UniProtKB:Q9H0C2}.
uniprot
proteins
transmembrane_region
TRANSMEM 21..50; /note="Helical; Name=1"; /evidence="ECO:0000250|UniProtKB:P02722"; TRANSMEM 88..112; /note="Helical; Name=2"; /evidence="ECO:0000250|UniProtKB:P02722"; TRANSMEM 123..143; /note="Helical; Name=3"; /evidence="ECO:0000250|UniProtKB:P02722"; TRANSMEM 192..212; /note="Helical; Name=4"; /evidence="ECO:0000250|UniProtKB:P02722"; TRANSMEM 224..244; /note="Helical; Name=5"; /evidence="ECO:0000250|UniProtKB:P02722"; TRANSMEM 285..302; /note="Helical; Name=6"; /evidence="ECO:0000250|UniProtKB:P02722
uniprot
proteins