PFKL
entrez
5211
canonical
uniprot
P17858
canonical
ensembl
ENSG00000141959
resolver
ensp
ENSP00000269848
resolver
ensp
ENSP00000555224
resolver
genesymbol
PFKL
resolver
hgnc
HGNC:8876
resolver
uniprot
Q7L2M7
resolver
uniprot
Q96A64
resolver
uniprot
Q96IH4
resolver
uniprot
Q9BR91
resolver
uniprot_entry
PFKAL_HUMAN
resolver
uniprot_entry
Q7L2M7_HUMAN
resolver
uniprot_sec
Q96A64
resolver
uniprot_sec
Q96IH4
resolver
uniprot_sec
Q9BR91
resolver
activity_regulation
ACTIVITY REGULATION: Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate. GlcNAcylation by OGT overcomes allosteric regulation. {ECO:0000255|HAMAP-Rule:MF_03184, ECO:0000269|PubMed:22923583}.
uniprot
proteins
amino_acid_sequence
MAAVDLEKLRASGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTIIGSARCKAFTTREGRRAAAYNLVQHGITNLCVIGGDGSLTGANIFRSEWGSLLEELVAEGKISETTARTYSHLNIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSSYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVTLSGNQSVRLPLMECVQMTKEVQKAMDDKRFDEATQLRGGSFENNWNIYKLLAHQKPPKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISHGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIHALLVVGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLVLRNEKCHDYYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWLSEKLREVYRKGRVFANAPDSACVIGLKKKAVAFSPVTELKKDTDFEHRMPREQWWLSLRLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDKGF
uniprot
proteins
ec_number
2.7.1.11
uniprot
proteins
function
FUNCTION: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity). {ECO:0000250|UniProtKB:P12382, ECO:0000255|HAMAP-Rule:MF_03184, ECO:0000269|PubMed:22923583}.
uniprot
proteins
mass_dalton
85018
uniprot
proteins
mutagenesis
MUTAGEN 527; /note="T->A: Does not affect GlcNAcylation."; /evidence="ECO:0000269|PubMed:22923583"; MUTAGEN 529; /note="S->A: Prevents GlcNAcylation and enhance enzyme activity."; /evidence="ECO:0000269|PubMed:22923583
uniprot
proteins
pathway_participation
PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. {ECO:0000255|HAMAP-Rule:MF_03184}.
uniprot
proteins
post_translational_modification
PTM: GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation. {ECO:0000269|PubMed:22923583}.
uniprot
proteins
protein_family
ATP-dependent PFK group I subfamily
uniprot
proteins
protein_family
Eukaryotic two domain clade 'E' sub-subfamily
uniprot
proteins
protein_family
Phosphofructokinase type A (PFKA) family
uniprot
proteins
pubmed
10830953
uniprot
proteins
pubmed
12665801
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
2139864
uniprot
proteins
pubmed
22223895
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
22923583
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
2533063
uniprot
proteins
sequence_length
780
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
uniprot
proteins