Rad51
entrez
19361
canonical
uniprot
Q08297
canonical
ensembl
ENSMUSG00000027323
resolver
ensp
ENSMUSP00000028795
resolver
enst
ENSMUST00000028795
resolver
genesymbol
Rad51
resolver
genesymbol_syn
Rad51a
resolver
genesymbol_syn
Reca
resolver
uniprot
Q3UAY5
resolver
uniprot_entry
RAD51_MOUSE
resolver
uniprot_sec
Q3UAY5
resolver
activity_regulation
ACTIVITY REGULATION: Acts as a self-inactivating ATPase, with rapid ATP hydrolysis and slow ADP dissociation. Ca(2+) by reducing the ATPase activity preserves the nucleoprotein filament in its active state, thereby stimulating the DNA strand exchange activity of RAD51. {ECO:0000250|UniProtKB:Q06609}.
uniprot
proteins
amino_acid_sequence
MAMQMQLEASADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGYHTVEAVAYAPKKELINIKGISEAKADKILTEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARGFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD
uniprot
proteins
ec_number
3.6.4.-
uniprot
proteins
function
FUNCTION: Homologous DNA recombinase that catalyzes strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:15834424). Binds to single-stranded DNA (ssDNA) in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (By similarity). Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template (By similarity). RAD51 targeting to ssDNA promotes removal of replication protein-A (RPA) from ssDNA and stabilization of RAD51-ssDNA filaments by blocking ATP hydrolysis (By similarity). Nucleosomal DNA is peeled from the histone surface by the RAD51 filament (By similarity). Recruited to resolve stalled replication forks during replication stress (By similarity). Also required for homologous recombination during meiosis by acting as an non-catalytic accessory factor for DMC1 recombinase, enabling proper chromosome pairing and crossing over (PubMed:32610038). Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 (By similarity). Also involved in interstrand cross-link repair (By similarity). {ECO:0000250|UniProtKB:Q06609, ECO:0000269|PubMed:15834424, ECO:0000269|PubMed:32610038}.
uniprot
proteins
mass_dalton
36971
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated by the SCF(FBH1) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA. Ubiquitinated by RFWD3 in response to DNA damage: ubiquitination leads to degradation by the proteasome, promoting homologous recombination. Deubiquitinated at Lys-57, Lys-58 and Lys-64 by UCHL3 in response to DNA damage, promoting interaction with BRCA2 and formation of nucleoprotein filaments. {ECO:0000250|UniProtKB:Q06609}.; PTM: Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylated at Ser-14 by PLK1, triggering phosphorylation at Thr-13 by CK2, thereby promoting interaction with TOPBP1 and recruitment to DNA damage sites during S-phase. Phosphorylation by ABL1 inhibits function. Phosphorylation at Ser-67 stimulates the formation of RAD51 nucleoprotein filament. {ECO:0000250|UniProtKB:Q06609}.
uniprot
proteins
protein_family
RAD51 subfamily
uniprot
proteins
protein_family
RecA family
uniprot
proteins
pubmed
12531026
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15834424
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
20976249
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22305526
uniprot
proteins
pubmed
24108124
uniprot
proteins
pubmed
30272023
uniprot
proteins
pubmed
30746471
uniprot
proteins
pubmed
30760716
uniprot
proteins
pubmed
30949703
uniprot
proteins
pubmed
32345962
uniprot
proteins
pubmed
32463460
uniprot
proteins
pubmed
32610038
uniprot
proteins
pubmed
35547804
uniprot
proteins
pubmed
39797737
uniprot
proteins
pubmed
8341671
uniprot
proteins
pubmed
8358431
uniprot
proteins
pubmed
8692798
uniprot
proteins
pubmed
9126738
uniprot
proteins
sequence_length
339
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:30272023, ECO:0000269|PubMed:30746471, ECO:0000269|PubMed:30760716, ECO:0000269|PubMed:30949703, ECO:0000269|PubMed:32463460, ECO:0000269|PubMed:32610038, ECO:0000269|PubMed:39797737}. Nucleus {ECO:0000269|PubMed:12531026}. Cytoplasm {ECO:0000269|PubMed:12531026}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q06609}. Mitochondrion matrix {ECO:0000250|UniProtKB:Q06609}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q06609}. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage (By similarity). DNA damage induces an increase in nuclear levels (By similarity). Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment (By similarity). Accumulated at sites of DNA damage in a SPIDR-dependent manner (By similarity). Recruited at sites of DNA damage in a MCM9-MCM8-dependent manner (By similarity). Recruited at sites of DNA damage following interaction with TOPBP1 in S-phase (By similarity). Colocalizes with ERCC5/XPG to nuclear foci in S phase (By similarity). Recruited to stalled replication forks during replication stress by the TONSL-MMS22L complex, as well as ATAD5 and WDR48 in an ATR-dependent manner (By similarity). In germ cells, during meiotic prcess, binds single-stranded DNA (ssDNA) away from the break site (PubMed:32610038). {ECO:0000250|UniProtKB:Q06609, ECO:0000269|PubMed:32610038}.
uniprot
proteins