Endog
entrez
13804
canonical
uniprot
O08600
canonical
ensembl
ENSMUSG00000015337
resolver
ensp
ENSMUSP00000015481
resolver
enst
ENSMUST00000015481
resolver
genesymbol
Endog
resolver
uniprot
Q3UN47
resolver
uniprot_entry
NUCG_MOUSE
resolver
uniprot_entry
Q3UN47_MOUSE
resolver
amino_acid_sequence
MRALRAGLTLALGAGLGAAAEHWRRREGKAPGLLGRVPLLPVVAADLPALPGGPAGGTGELAKYGLPGVAQLRSRESYVLSYDPRTRGALWVLEQLRPERLRGDGDRSACDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQRAMDDTFYLSNVAPQVPHLNQNAWNNLERYSRSLTRTYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRSYVMPNAPVDETIPLERFLVPIESIERASGLLFVPNILARAGNLKAITAGSK
uniprot
proteins
ec_number
3.1.30.-
uniprot
proteins
function
FUNCTION: Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (PubMed:25355512, PubMed:32095813). The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (PubMed:32095813). Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs (PubMed:32095813). Promotes conservative recombination when the DNA is 5hmC-modified (PubMed:25355512). Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response (PubMed:33473107). GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation (By similarity). Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication (By similarity). {ECO:0000250|UniProtKB:Q14249, ECO:0000269|PubMed:25355512, ECO:0000269|PubMed:32095813, ECO:0000269|PubMed:33473107}.
uniprot
proteins
mass_dalton
32191
uniprot
proteins
mutagenesis
MUTAGEN 107; /note="R->Q: Loss of deoxyribonuclease activity."; /evidence="ECO:0000269|PubMed:32192768"; MUTAGEN 110; /note="C->A: Loss of homodimerization and deoxyribonuclease activity. Shows a 1.3-fold higher rate for the cleavage of the 5hmC-modified DNA junction over unmodified junction, as compared to the 1.8-fold difference seen in the wild type enzyme."; /evidence="ECO:0000269|PubMed:32095813, ECO:0000269|PubMed:32192768"; MUTAGEN 110; /note="C->S: Shows a 2.1-fold higher rate for the cleavage of the 5hmC-modified DNA junction over unmodified junction, as compared to the 1.8-fold difference seen in the wild type enzyme."; /evidence="ECO:0000269|PubMed:32095813"; MUTAGEN 138; /note="H->A: Catalytically inactive."; /evidence="ECO:0000269|PubMed:32095813, ECO:0000269|PubMed:32192768
uniprot
proteins
post_translational_modification
PTM: GSK3-beta-mediated phosphorylation at Thr-125 is necessary for its interaction with YWHAG and the induction of autophagy. {ECO:0000250|UniProtKB:Q14249}.
uniprot
proteins
protein_family
DNA/RNA non-specific endonuclease family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
25355512
uniprot
proteins
pubmed
32095813
uniprot
proteins
pubmed
32192768
uniprot
proteins
pubmed
33473107
uniprot
proteins
pubmed
9324313
uniprot
proteins
sequence_length
294
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q14249}.
uniprot
proteins