Rnf4
entrez
19822
canonical
uniprot
Q9QZS2
canonical
ensembl
ENSMUSG00000029110
resolver
ensp
ENSMUSP00000030992
resolver
ensp
ENSMUSP00000138411
resolver
ensp
ENSMUSP00000138555
resolver
enst
ENSMUST00000030992
resolver
enst
ENSMUST00000182047
resolver
enst
ENSMUST00000182709
resolver
genesymbol
Rnf4
resolver
uniprot
O35941
resolver
uniprot
Q541Z6
resolver
uniprot_entry
RNF4_MOUSE
resolver
uniprot_sec
O35941
resolver
uniprot_sec
Q541Z6
resolver
amino_acid_sequence
MSTRNPQRKRRGGTVNSRQTQKRTRETTSTPEVSLETEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSRDKDVYVTTHTPRSTKDDGATGPRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
function
FUNCTION: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation (PubMed:20681948). Regulates the degradation of several proteins including PML and the transcriptional activator PEA3 (By similarity). Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation (By similarity). Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 (By similarity). Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation (By similarity). Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97 (By similarity). {ECO:0000250|UniProtKB:P78317, ECO:0000269|PubMed:20681948}.
uniprot
proteins
mass_dalton
21911
uniprot
proteins
mutagenesis
MUTAGEN 134; /note="V->E: Abolishes homodimerization. Strongly reduced E3 ubiquitin ligase activity."; /evidence="ECO:0000269|PubMed:20681948"; MUTAGEN 155; /note="S->A: No effect."; /evidence="ECO:0000269|PubMed:20681948"; MUTAGEN 155; /note="S->E: Abolishes homodimerization. Strongly reduced E3 ubiquitin ligase activity."; /evidence="ECO:0000269|PubMed:20681948"; MUTAGEN 161; /note="V->A: Abolishes homodimerization. Strongly reduced E3 ubiquitin ligase activity."; /evidence="ECO:0000269|PubMed:20681948"; MUTAGEN 193; /note="Y->A: Abolishes homodimerization. Loss of E3 ubiquitin ligase activity."; /evidence="ECO:0000269|PubMed:20681948
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:20681948}.
uniprot
proteins
post_translational_modification
PTM: Sumoylated; conjugated by one or two SUMO1 moieties. {ECO:0000250|UniProtKB:O88846}.; PTM: Autoubiquitinated. {ECO:0000269|PubMed:20681948}.
uniprot
proteins
pubmed
10822263
uniprot
proteins
pubmed
10849425
uniprot
proteins
pubmed
12885770
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17242355
uniprot
proteins
pubmed
19779455
uniprot
proteins
pubmed
20681948
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
9479498
uniprot
proteins
sequence_length
194
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10822263}. Nucleus {ECO:0000269|PubMed:10822263, ECO:0000269|PubMed:10849425}. Nucleus, PML body {ECO:0000250|UniProtKB:P78317}.
uniprot
proteins