Gnat3
entrez
286924
canonical
uniprot
P29348
canonical
ensembl
ENSRNOG00000005268
resolver
ensembl
ENSRNOG00055022039
resolver
ensembl
ENSRNOG00060012788
resolver
ensembl
ENSRNOG00065017170
resolver
ensp
ENSRNOP00000007032
resolver
ensp
ENSRNOP00055030898
resolver
ensp
ENSRNOP00060017215
resolver
ensp
ENSRNOP00065022683
resolver
enst
ENSRNOT00000007032
resolver
enst
ENSRNOT00055037856
resolver
enst
ENSRNOT00060021750
resolver
enst
ENSRNOT00065028635
resolver
genesymbol
Gnat3
resolver
genesymbol_syn
Gnat-3
resolver
uniprot_entry
GNAT3_RAT
resolver
amino_acid_sequence
MGSGISSESKESAKRSKELEKKLQEDAERDARTVKLLLLGAGESGKSTIVKQMKIIHKNGYSKQECMEFKAVVYSNTLQSILAIVKAMTTLGIDYVNPRSREDQQLLLSMANTLEDGDMTPQLAEIIKRLWGDPGIQACFERASEYQLNDSAAYYLNDLDRLTAPGYVPNEQDVLHSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDEEVNRMHESLHLFNSICNHKYFATTSIVLFLNKKDLFQEKVTKVHLSICFPEYTGPNTFEDAGNYIKNQFLDLNLKKEDKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF
uniprot
proteins
function
FUNCTION: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction (PubMed:1608467, PubMed:7626029, PubMed:7596440, PubMed:10052456, PubMed:11447270). Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors (By similarity). Also functions as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications for the prevention and treatment of malabsorption syndromes and diet-related disorders including diabetes and obesity. {ECO:0000250|UniProtKB:A8MTJ3, ECO:0000269|PubMed:10052456, ECO:0000269|PubMed:11447270, ECO:0000269|PubMed:1608467, ECO:0000269|PubMed:7596440, ECO:0000269|PubMed:7626029}.
uniprot
proteins
mass_dalton
40294
uniprot
proteins
mutagenesis
MUTAGEN 352; /note="G->P: Loss of activation by both bitter and sweet compounds due to disruption of interaction with taste receptors."; /evidence="ECO:0000269|PubMed:11447270
uniprot
proteins
post_translational_modification
PTM: Potential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane.
uniprot
proteins
protein_family
G(i/o/t/z) subfamily
uniprot
proteins
protein_family
G-alpha family
uniprot
proteins
pubmed
10052456
uniprot
proteins
pubmed
10407021
uniprot
proteins
pubmed
10576254
uniprot
proteins
pubmed
10940948
uniprot
proteins
pubmed
11447270
uniprot
proteins
pubmed
15561439
uniprot
proteins
pubmed
15654652
uniprot
proteins
pubmed
1608467
uniprot
proteins
pubmed
17021831
uniprot
proteins
pubmed
7596440
uniprot
proteins
pubmed
7626029
uniprot
proteins
pubmed
8692869
uniprot
proteins
pubmed
9092606
uniprot
proteins
sequence_length
354
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10940948}. Note=Associated with microvilli, the presumed sites of sensory transduction in taste cells.
uniprot
proteins