Alox12b
entrez
11686
canonical
uniprot
O70582
canonical
ensembl
ENSMUSG00000032807
resolver
ensp
ENSMUSP00000035250
resolver
enst
ENSMUST00000036424
resolver
genesymbol
Alox12b
resolver
genesymbol_syn
Aloxe2
resolver
uniprot
Q2KHL0
resolver
uniprot_entry
LX12B_MOUSE
resolver
uniprot_entry
Q2KHL0_MOUSE
resolver
activity_regulation
ACTIVITY REGULATION: Increased by calcium. {ECO:0000269|PubMed:11256953}.
uniprot
proteins
amino_acid_sequence
MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLHKEPHSFLAKDPWYCNYVQICAPDCRVYHFPAYQWMDGYETLALREATGKITADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWDGYIPGFPILINIKATRFLNSNLRFSFVKTASFFYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKSVVSEYVAEHWTEDSFFGYQYLNGINPGLIRRCTQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIPTVELNGQQQHHCAPMCLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLGRESSGFPTCLRTIPELIEYVTMVMYTCSARHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMDTLPDVKTTCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIRQRNKCLTLPYYYLDPVLIENSISI
uniprot
proteins
ec_number
1.13.11.-
uniprot
proteins
function
FUNCTION: Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:16129665). Does not convert arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE (PubMed:10100631, PubMed:11256953). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:17403930, PubMed:17429434, PubMed:21558561). May also play a role in the regulation of the expression of airway mucins (By similarity). {ECO:0000250|UniProtKB:O75342, ECO:0000269|PubMed:10100631, ECO:0000269|PubMed:11256953, ECO:0000269|PubMed:16129665, ECO:0000269|PubMed:17403930, ECO:0000269|PubMed:17429434, ECO:0000269|PubMed:21558561}.
uniprot
proteins
mass_dalton
80578
uniprot
proteins
mutagenesis
MUTAGEN 390; /note="F->A: Reduced enzymatic activity and altered stereoselectivity of the oxygenation reaction."; /evidence="ECO:0000269|PubMed:16129665"; MUTAGEN 390; /note="F->W: Loss of enzymatic activity."; /evidence="ECO:0000269|PubMed:16129665"; MUTAGEN 441; /note="G->A: Reduced enzymatic activity and changed stereoselectivity of the oxygenation reaction."; /evidence="ECO:0000269|PubMed:16129665"; MUTAGEN 441; /note="G->V: Loss of enzymatic activity."; /evidence="ECO:0000269|PubMed:16129665"; MUTAGEN 455; /note="A->I,W: Reduced enzymatic activity and altered stereoselectivity of the oxygenation reaction."; /evidence="ECO:0000269|PubMed:16129665"; MUTAGEN 631; /note="V->A,G: Increased enzymatic activity and changed stereoselectivity of the oxygenation reaction to produce (11R)-HPETE preferentially instead of (12R)-HPETE."; /evidence="ECO:0000269|PubMed:16129665
uniprot
proteins
pathway_participation
PATHWAY: Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.; PATHWAY: Lipid metabolism; sphingolipid metabolism.
uniprot
proteins
protein_family
Lipoxygenase family
uniprot
proteins
pubmed
10100631
uniprot
proteins
pubmed
11256953
uniprot
proteins
pubmed
16129665
uniprot
proteins
pubmed
17403930
uniprot
proteins
pubmed
17429434
uniprot
proteins
pubmed
21558561
uniprot
proteins
pubmed
9518531
uniprot
proteins
pubmed
9837935
uniprot
proteins
sequence_length
701
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00726}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:9837935}.
uniprot
proteins