Hmces
entrez
232210
canonical
uniprot
Q8R1M0
canonical
ensembl
ENSMUSG00000030060
resolver
ensp
ENSMUSP00000032141
resolver
ensp
ENSMUSP00000109236
resolver
enst
ENSMUST00000032141
resolver
enst
ENSMUST00000113606
resolver
genesymbol
Hmces
resolver
genesymbol_syn
Srap1
resolver
genesymbol_syn
Srapd1
resolver
uniprot_entry
HMCES_MOUSE
resolver
activity_regulation
ACTIVITY REGULATION: Formation and reversal of DNA-protein cross-link depends on DNA context. Catalyzes formation of the thiazolidine linkage in presence of abasic sites in single-stranded DNA. Mediates the reversal of the thiazolidine cross-link in presence of double stranded DNA. {ECO:0000250|UniProtKB:Q96FZ2}.
uniprot
proteins
amino_acid_sequence
MCGRTSCHLPREVLTRACAYQDRQGRRRLPQWRDPDKYCPSYNKSPQSSSPVLLSRLHFEKDADSSDRIIIPMRWGLVPSWFKESDPSKLQFNTTNCRSDTIMEKQSFKVPLGKGRRCVVLADGFYEWQRCQGTNQRQPYFIYFPQIKTEKSGGNDASDSSDNKEKVWDNWRLLTMAGIFDCWEAPGGECLYSYSIITVDSCRGLSDIHSRMPAILDGEEAVSKWLDFGEVATQEALKLIHPIDNITFHPVSPVVNNSRNNTPECLAPADLLVKKEPKANGSSQRMMQWLATKSPKKEVPDSPKKDASGLPQWSSQFLQKSPLPAKRGATSSFLDRWLKQEKEDEPMAKKPNS
uniprot
proteins
ec_number
3.4.-.-
uniprot
proteins
ec_number
4.-.-.-
uniprot
proteins
function
FUNCTION: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (By similarity). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (By similarity). Promotes error-free repair by protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (By similarity). The HMCES DNA-protein cross-link is then either reversed or degraded (By similarity). HMCES is able to catalyze the reversal of its thiazolidine cross-link and cycle between a cross-link and a non-cross-linked state depending on DNA context: mediates self-reversal of the thiazolidine cross-link in double stranded DNA, allowing APEX1 to initiate downstream repair of abasic sites (By similarity). The HMCES DNA-protein cross-link can also be degraded by the SPRTN metalloprotease following unfolding by the BRIP1/FANCJ helicase (By similarity). Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction (By similarity). Plays a protective role during somatic hypermutation of immunoglobulin genes in B-cells: acts via its ability to form covalent cross-links with abasic sites, thereby limiting the accumulation of deletions in somatic hypermutation target regions (PubMed:35450882). Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA-protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway (PubMed:31806351). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (PubMed:29020633). {ECO:0000250|UniProtKB:Q96FZ2, ECO:0000269|PubMed:29020633, ECO:0000269|PubMed:31806351, ECO:0000269|PubMed:35450882}.
uniprot
proteins
mass_dalton
40169
uniprot
proteins
mutagenesis
MUTAGEN 2; /note="C->A: Accumulation of uncleaved form with the N-terminal methionine."; /evidence="ECO:0000269|PubMed:29020633"; MUTAGEN 209; /note="H->Q: Accumulation of uncleaved form with the N-terminal methionine."; /evidence="ECO:0000269|PubMed:29020633"; MUTAGEN 289..290; /note="WL->GA: Stimulates cleavage of the N-terminal methionine."; /evidence="ECO:0000269|PubMed:29020633
uniprot
proteins
protein_family
SOS response-associated peptidase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
23434322
uniprot
proteins
pubmed
29020633
uniprot
proteins
pubmed
31806351
uniprot
proteins
pubmed
35450882
uniprot
proteins
sequence_length
353
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Chromosome {ECO:0000250|UniProtKB:Q96FZ2}. Note=Recruited to chromatin following DNA damage. Localizes to replication forks. {ECO:0000250|UniProtKB:Q96FZ2}.
uniprot
proteins