Padi4
entrez
18602
canonical
uniprot
Q9Z183
canonical
ensembl
ENSMUSG00000025330
resolver
ensp
ENSMUSP00000026381
resolver
enst
ENSMUST00000026381
resolver
genesymbol
Padi4
resolver
genesymbol_syn
Pad4
resolver
genesymbol_syn
Pdi4
resolver
uniprot
A2AMU3
resolver
uniprot
Q75WC7
resolver
uniprot_entry
PADI4_MOUSE
resolver
uniprot_sec
A2AMU3
resolver
uniprot_sec
Q75WC7
resolver
activity_regulation
ACTIVITY REGULATION: Strongly Inhibited by F-amidine and N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide (Cl-amidine) (PubMed:24463520). These inhibitors are however not specific to PADI4 and also inhibit other members of the family (PubMed:24463520). Specifically inhibited by GSK215 small molecule; inhibiting formation of neutrophil extracellular traps (NETs) (PubMed:25622091). {ECO:0000269|PubMed:24463520, ECO:0000269|PubMed:25622091}.
uniprot
proteins
amino_acid_sequence
MAQGAVIHVAPEQPTHAVCVVGTATPLDVRGSAPKGYTTFGITASPGVIVDVIHGPPVKKSTMGASKWPLDPELEVTLQVKAASSRTDDEKVRVSYYGPKTSPVQALIYITGVELSLSADVTRTGRVKPAQAGKDQSTWTWGPGGRGAILLVNCDKEDPQASGMDFEDDKILDNKDLQDMSPMTLSTKTPKDFFEKYQLVLEVPKAKMNRVRVFRATRGKLPSRYKVALGPQQFSYCLELPGGQHSTDFYVEGLAFPDADFKGLIPLTISLLDKSNPELPEALVFQDSVTFRVAPWIMTPNTQPPQEVYVCRVSDNEDFLKSLATLTKKAKCKLTVCPEEENIDDQWMQDEMEIGYIQAPHKTLPVVFDSPRDRGLKDFPVKRVMGPNFGYVTRKLYMSELTGLDAFGNLEVSPPVTVRGKEYPLGRILIGNSGYSSSESRDMHQALQDFLSAQQVQAPVRLFSDWLFVGHVDEFLSFVPARDKQGFRLLLSSPRACYQLFQELQSQGHGEATLFEGLKRKRQTINEILSNKKLRDQNAYVESCIDWNRAVLKRELGLAEGDIIDIPQLFKLAGNSRGNSKAQAFFPNMVNMLVLGKYLGIPKPFGPIIDGHCCLEEEVRSHLEPLGLHCTFINDFYTYHVYNGEVHCGTNVRRKPFTFKWWHMVP
uniprot
proteins
ec_number
3.5.3.15
uniprot
proteins
function
FUNCTION: Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:32528174). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:24463520, PubMed:32528174). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:20733033, PubMed:23650392). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (By similarity). Involved for the formation of neutrophil extracellular traps (NETs) by mediating citrullination of histone H3 and initiating decondensation of chromatin and nuclear membrane rupture (PubMed:20733033, PubMed:32528174, PubMed:32170015, PubMed:25622091). NETs are mainly composed of DNA fibers and are released by neutrophils to trap pathogens during inflammation (PubMed:20733033, PubMed:32528174, PubMed:32170015, PubMed:25622091). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (By similarity). {ECO:0000250|UniProtKB:Q9UM07, ECO:0000269|PubMed:15339660, ECO:0000269|PubMed:20733033, ECO:0000269|PubMed:23650392, ECO:0000269|PubMed:24463520, ECO:0000269|PubMed:25622091, ECO:0000269|PubMed:32170015, ECO:0000269|PubMed:32528174}.
uniprot
proteins
mass_dalton
74415
uniprot
proteins
post_translational_modification
PTM: Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme. {ECO:0000250|UniProtKB:Q9UM07}.
uniprot
proteins
protein_family
Protein arginine deiminase family
uniprot
proteins
pubmed
10092850
uniprot
proteins
pubmed
15087120
uniprot
proteins
pubmed
15339660
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
20733033
uniprot
proteins
pubmed
23650392
uniprot
proteins
pubmed
24463520
uniprot
proteins
pubmed
25622091
uniprot
proteins
pubmed
32170015
uniprot
proteins
pubmed
32528174
uniprot
proteins
sequence_length
666
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9UM07}. Nucleus {ECO:0000250|UniProtKB:Q9UM07}. Cytoplasmic granule {ECO:0000250|UniProtKB:Q9UM07}. Note=Cytoplasmic granules of eosinophils and neutrophils. {ECO:0000250|UniProtKB:Q9UM07}.
uniprot
proteins