EMC2
entrez
9694
canonical
uniprot
Q15006
canonical
ensembl
ENSG00000104412
resolver
ensp
ENSP00000220853
resolver
ensp
ENSP00000560486
resolver
enst
ENST00000220853
resolver
genesymbol
EMC2
resolver
genesymbol_syn
KIAA0103
resolver
genesymbol_syn
TTC35
resolver
hgnc
HGNC:28963
resolver
uniprot
Q8WUE1
resolver
uniprot_entry
EMC2_HUMAN
resolver
uniprot_sec
Q8WUE1
resolver
amino_acid_sequence
MAKVSELYDVTWEEMRDKMRKWREENSRNSEQIVEVGEELINEYASKLGDDIWIIYEQVMIAALDYGRDDLALFCLQELRRQFPGSHRVKRLTGMRFEAMERYDDAIQLYDRILQEDPTNTAARKRKIAIRKAQGKNVEAIRELNEYLEQFVGDQEAWHELAELYINEHDYAKAAFCLEELMMTNPHNHLYCQQYAEVKYTQGGLENLELSRKYFAQALKLNNRNMRALFGLYMSASHIASNPKASAKTKKDNMKYASWAASQINRAYQFAGRSKKETKYSLKAVEDMLETLQITQS
uniprot
proteins
function
FUNCTION: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242231, PubMed:29809151, PubMed:30415835, PubMed:32439656, PubMed:32459176, PubMed:33964204). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:29242231, PubMed:29809151, PubMed:30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:29809151, PubMed:30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29242231, PubMed:29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). {ECO:0000269|PubMed:29242231, ECO:0000269|PubMed:29809151, ECO:0000269|PubMed:30415835, ECO:0000269|PubMed:32439656, ECO:0000269|PubMed:32459176, ECO:0000269|PubMed:33964204, ECO:0000305}.
uniprot
proteins
mass_dalton
34834
uniprot
proteins
mutagenesis
MUTAGEN 28; /note="R->A: Loss of interaction with EMC5."; /evidence="ECO:0000269|PubMed:32439656"; MUTAGEN 61; /note="I->K: No effect on transmembrane domain binding of tail-anchored proteins; when associated with K-95 and E-122."; /evidence="ECO:0000269|PubMed:32459176"; MUTAGEN 95; /note="M->K: No effect on transmembrane domain binding of tail-anchored proteins; when associated with K-61 and E-122."; /evidence="ECO:0000269|PubMed:32459176"; MUTAGEN 122; /note="A->E: No effect on transmembrane domain binding of tail-anchored proteins; when associated with K-61 and K-95."; /evidence="ECO:0000269|PubMed:32459176"; MUTAGEN 156; /note="E->A: Loss of interaction with EMC5."; /evidence="ECO:0000269|PubMed:32439656"; MUTAGEN 160; /note="E->A: Loss of interaction with EMC5."; /evidence="ECO:0000269|PubMed:32439656"; MUTAGEN 171; /note="Y->A: Decreased interaction with EMC5 and EMC8."; /evidence="ECO:0000269|PubMed:32439656"; MUTAGEN 180; /note="E->A: Decreased interaction with EMC3."; /evidence="ECO:0000269|PubMed:32439656"; MUTAGEN 189..191; /note="HLY->EEK: Decreased transmembrane domain binding of tail-anchored proteins."; /evidence="ECO:0000269|PubMed:32459176"; MUTAGEN 193..194; /note="QQ->EK: No effect on transmembrane domain binding of tail-anchored proteins."; /evidence="ECO:0000269|PubMed:32459176"; MUTAGEN 200; /note="Y->A: Decreased interaction with EMC5 and EMC8."; /evidence="ECO:0000269|PubMed:32439656"; MUTAGEN 227; /note="R->A: Loss of interaction with EMC5 and EMC8."; /evidence="ECO:0000269|PubMed:32439656"; MUTAGEN 259; /note="W->A: Decreased interaction with EMC3."; /evidence="ECO:0000269|PubMed:32439656
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated when soluble in the cytoplasm, leading to its degradation by the proteasome (PubMed:33964204). Interaction with EMC2 prevents its ubiquitination and degradation (PubMed:33964204). {ECO:0000269|PubMed:33964204}.
uniprot
proteins
protein_family
EMC2 family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
19608861
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22119785
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
25944712
uniprot
proteins
pubmed
29242231
uniprot
proteins
pubmed
29809151
uniprot
proteins
pubmed
30415835
uniprot
proteins
pubmed
32439656
uniprot
proteins
pubmed
32459176
uniprot
proteins
pubmed
33964204
uniprot
proteins
pubmed
7788527
uniprot
proteins
sequence_length
297
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:22119785}; Peripheral membrane protein {ECO:0000269|PubMed:32439656, ECO:0000269|PubMed:32459176}; Cytoplasmic side {ECO:0000269|PubMed:22119785, ECO:0000269|PubMed:32439656}. Note=May also localize to the nuclear envelope. {ECO:0000250|UniProtKB:Q9CRD2}.
uniprot
proteins