NUDT3
entrez
11165
canonical
uniprot
O95989
canonical
ensembl
ENSG00000272325
resolver
ensp
ENSP00000476119
resolver
enst
ENST00000607016
resolver
genesymbol
NUDT3
resolver
genesymbol_syn
DIPP
resolver
genesymbol_syn
DIPP1
resolver
hgnc
HGNC:8050
resolver
uniprot
B2R8N4
resolver
uniprot_entry
NUDT3_HUMAN
resolver
uniprot_sec
B2R8N4
resolver
activity_regulation
ACTIVITY REGULATION: Endopolyphospahatase activity is inhibited by NaF, NaPPi, beta-glycerol phosphate and heparin (PubMed:34788624). 5-diphosphoinositol pentakisphosphate (5-InsP7) inhibits its mRNA decapping activity (PubMed:32727897). {ECO:0000269|PubMed:32727897, ECO:0000269|PubMed:34788624}.
uniprot
proteins
amino_acid_sequence
MMKLKSNQTRTYDGDGYKKRAACLCFRSESEEEVLLVSSSRHPDRWIVPGGGMEPEEEPSVAAVREVCEEAGVKGTLGRLVGIFENQERKHRTYVYVLIVTEVLEDWEDSVNIGRKREWFKIEDAIKVLQYHKPVQASYFETLRQGYSANNGTPVVATTYSVSAQSSMSGIR
uniprot
proteins
ec_number
3.6.1.10
uniprot
proteins
ec_number
3.6.1.52
uniprot
proteins
ec_number
3.6.1.59
uniprot
proteins
ec_number
3.6.1.61
uniprot
proteins
ec_number
3.6.1.62
uniprot
proteins
function
FUNCTION: Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction (PubMed:10585413, PubMed:12370170, PubMed:9822604). InsP6 (inositol hexakisphosphate) is not a substrate (PubMed:9822604). Acts as a negative regulator of the ERK1/2 pathway (By similarity). Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A) and diadenosine 5',5'''- P1,P5-pentaphosphate (Ap5A) being the preferred substrates (PubMed:10419486, PubMed:12370170). The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A (PubMed:12370170). Also able to hydrolyze 5-phosphoribose 1-diphosphate (PubMed:12370170). Acts as a decapping enzyme that modulates the stability of a subset of mRNAs implicated in cell motility (PubMed:26932476). Hydrolyzes monomethylated capped RNA after both the alpha- and beta-phosphates generating m7GMP + ppRNA and m7GDP + pRNA (PubMed:32727897). Can hydrolyze unmethylated capped RNAs (By similarity). Divalent cations zinc, magnesium and manganese determine its substrate specificity (PubMed:34788624). Exhibits diphosphoinositol polyphosphate phosphohydrolase in the presence of magnesium ions, diadenosine hexaphosphate hydrolase activity in the presence of manganese ions and endopolyphosphatase activity in the presence of zinc ions (PubMed:34788624). Plays an important role in limiting DNA damage and maintaining cell survival upon oxidative stress via its endopolyphosphatase activity (PubMed:34788624). {ECO:0000250|UniProtKB:Q9JI46, ECO:0000269|PubMed:10419486, ECO:0000269|PubMed:10585413, ECO:0000269|PubMed:12370170, ECO:0000269|PubMed:34788624, ECO:0000269|PubMed:9822604, ECO:0000305|PubMed:26932476}.
uniprot
proteins
mass_dalton
19471
uniprot
proteins
mutagenesis
MUTAGEN 50; /note="G->A,V: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 51; /note="G->A: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 52; /note="G->A,V: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 66; /note="E->Q: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 69..70; /note="EE->QQ: Loss of mRNA-decapping activity."; /evidence="ECO:0000269|PubMed:26932476"; MUTAGEN 70; /note="E->A: Loss of endopolyphosphatase activity."; /evidence="ECO:0000269|PubMed:34788624"; MUTAGEN 70; /note="E->Q: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:9822604"; MUTAGEN 72; /note="G->A: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 75; /note="G->A: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 78; /note="G->A: No effect on diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 78; /note="G->V: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 82; /note="G->A: Loss of diphosphoinositol polyphosphate phosphohydrolase activity."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 84; /note="F->Y: Induces a strong decrease in Ap6A and [PP]-InsP4 hydrolysis, while it only weakly affects PP-InsP5 hydrolysis."; /evidence="ECO:0000269|PubMed:10585413"; MUTAGEN 91; /note="H->L: Induces a strong decrease in Ap6A and [PP]-InsP4 hydrolysis, while it only weakly affects PP-InsP5 hydrolysis."; /evidence="ECO:0000269|PubMed:10585413
uniprot
proteins
protein_family
DIPP subfamily
uniprot
proteins
protein_family
Nudix hydrolase family
uniprot
proteins
pubmed
10419486
uniprot
proteins
pubmed
10585413
uniprot
proteins
pubmed
12370170
uniprot
proteins
pubmed
14574404
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
19585659
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
26932476
uniprot
proteins
pubmed
32727897
uniprot
proteins
pubmed
34788624
uniprot
proteins
pubmed
9822604
uniprot
proteins
sequence_length
172
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:34788624}. Nucleus {ECO:0000269|PubMed:34788624}.
uniprot
proteins