Ngly1
entrez
361014
canonical
uniprot
Q5XI55
canonical
ensembl
ENSRNOG00000006143
resolver
ensembl
ENSRNOG00055014194
resolver
ensembl
ENSRNOG00060020893
resolver
ensembl
ENSRNOG00065009141
resolver
ensp
ENSRNOP00000008289
resolver
ensp
ENSRNOP00000083143
resolver
ensp
ENSRNOP00000086683
resolver
enst
ENSRNOT00000116474
resolver
genesymbol
Ngly1
resolver
uniprot
A0A8I6A5T0
resolver
uniprot_entry
A0A8I6A5T0_RAT
resolver
uniprot_entry
NGLY1_RAT
resolver
activity_regulation
ACTIVITY REGULATION: Inhibited by Z-VAD-fmk, a well-known caspase inhibitor, which inhibits enzyme activity through covalent binding of the carbohydrate to the single Cys-306 residue. {ECO:0000250}.
uniprot
proteins
amino_acid_sequence
MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAVERRSRLDGSSQKVEFSQHPAAVRLPAEQPEDPTGLMQHSGNQPGQPLSLPSAPLVVGDSTIFKVLQSNIQHVQLYENPVLQEKALACIPVNELKRKSQEKLFRARKLDKGTKVSDEDFLLLELLHWFKEEFFHWVNNVVCSRCGRETRSRDEALPPNDDELKWGAKNVEDHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHEEVMSRRTKVKEELLRETINGLNKQRQLLLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETCLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNANISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDNVSIRTSSQSFETGSVRWKLRSEMAQVNLLGDRNLRSYDDFCGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDHAENGLEIIITFSDL
uniprot
proteins
ec_number
3.5.1.52
uniprot
proteins
function
FUNCTION: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity). {ECO:0000250}.
uniprot
proteins
mass_dalton
74677
uniprot
proteins
protein_family
PNGase family
uniprot
proteins
protein_family
Transglutaminase-like superfamily
uniprot
proteins
pubmed
15057822
uniprot
proteins
pubmed
15489334
uniprot
proteins
sequence_length
651
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
uniprot
proteins