Sphk1
entrez
170897
canonical
uniprot
Q91V26
canonical
ensembl
ENSRNOG00000010626
resolver
ensp
ENSRNOP00000072525
resolver
enst
ENSRNOT00000083593
resolver
genesymbol
Sphk1
resolver
uniprot
A0A0G2K373
resolver
uniprot
Q642F6
resolver
uniprot_entry
A0A0G2K373_RAT
resolver
uniprot_entry
SPHK1_RAT
resolver
uniprot_sec
Q642F6
resolver
activity_regulation
ACTIVITY REGULATION: Acetyltransferase activity increases in presence of the kinase substrate, sphingosine (By similarity). In Purkinje cells, kinase activity on sphingosine increases in presence of VEGFA (By similarity). In neurons, kinase activity increases during the first 24h in presence of Amyloid-beta protein 42 to decrease after 96h (PubMed:26334640). {ECO:0000250|UniProtKB:Q8CI15, ECO:0000250|UniProtKB:Q9NYA1, ECO:0000269|PubMed:26334640}.
uniprot
proteins
amino_acid_sequence
MQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCEAGVMHLFYIRAGVSRAMLVRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI
uniprot
proteins
ec_number
2.3.1.-
uniprot
proteins
ec_number
2.7.1.91
uniprot
proteins
function
FUNCTION: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (Probable) (PubMed:16118219). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (By similarity). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion (PubMed:28284343). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (By similarity). {ECO:0000250|UniProtKB:Q9NYA1, ECO:0000269|PubMed:16118219, ECO:0000269|PubMed:28284343, ECO:0000305|PubMed:26334640}.; FUNCTION: Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. {ECO:0000250|UniProtKB:Q8CI15}.
uniprot
proteins
mass_dalton
42418
uniprot
proteins
mutagenesis
MUTAGEN 82; /note="G->D: Loss of sphingosine kinase activity."; /evidence="ECO:0000269|PubMed:16118219
uniprot
proteins
pubmed
11560121
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16118219
uniprot
proteins
pubmed
26334640
uniprot
proteins
pubmed
28284343
uniprot
proteins
sequence_length
383
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16118219}. Nucleus {ECO:0000250|UniProtKB:Q9NYA1}. Cell membrane {ECO:0000250|UniProtKB:Q9NYA1}. Endosome membrane {ECO:0000250|UniProtKB:Q9NYA1}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9NYA1}. Membrane, clathrin-coated pit {ECO:0000250|UniProtKB:Q9NYA1}. Synapse {ECO:0000250|UniProtKB:Q8CI15}. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner. Binds to membranes containing negatively charged lipids but not neutral lipids (By similarity). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity). {ECO:0000250|UniProtKB:Q8CI15, ECO:0000250|UniProtKB:Q9NYA1}.
uniprot
proteins