Cyp27b1
entrez
13115
canonical
uniprot
O35084
canonical
ensembl
ENSMUSG00000006724
resolver
ensp
ENSMUSP00000130005
resolver
enst
ENSMUST00000165764
resolver
genesymbol
Cyp27b1
resolver
genesymbol_syn
Cyp27b
resolver
genesymbol_syn
Cyp40
resolver
uniprot
Q66JY8
resolver
uniprot_entry
CP27B_MOUSE
resolver
uniprot_entry
Q66JY8_MOUSE
resolver
activity_regulation
ACTIVITY REGULATION: Activated by cardiolipin and dioleoyl phosphatidylethanolamine (DOPE), phospholipids found in the inner mitochondrial membrane. Inhibited by high substrate concentration. {ECO:0000250|UniProtKB:O15528}.
uniprot
proteins
amino_acid_sequence
MTQAVKLASRVFHRIHLPLQLDASLGSRGSESVLRSLSDIPGPSTLSFLAELFCKGGLSRLHELQVHGAARYGPIWSGSFGTLRTVYVADPTLVEQLLRQESHCPERCSFSSWAEHRRRHQRACGLLTADGEEWQRLRSLLAPLLLRPQAAAGYAGTLDNVVRDLVRRLRRQRGRGSGLPGLVLDVAGEFYKFGLESIGAVLLGSRLGCLEAEVPPDTETFIHAVGSVFVSTLLTMAMPNWLHHLIPGPWARLCRDWDQMFAFAQRHVELREGEAAMRNQGKPEEDMPSGHHLTHFLFREKVSVQSIVGNVTELLLAGVDTVSNTLSWTLYELSRHPDVQTALHSEITAGTRGSCAHPHGTALSQLPLLKAVIKEVLRLYPVVPGNSRVPDRDIRVGNYVIPQDTLVSLCHYATSRDPTQFPDPNSFNPARWLGEGPTPHPFASLPFGFGKRSCIGRRLAELELQMALSQILTHFEVLPEPGALPIKPMTRTVLVPERSINLQFVDR
uniprot
proteins
ec_number
1.14.15.18
uniprot
proteins
function
FUNCTION: A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) (PubMed:10092858, PubMed:15972816). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2 (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:10092858, PubMed:15972816). {ECO:0000250|UniProtKB:O15528, ECO:0000269|PubMed:10092858, ECO:0000269|PubMed:15972816}.
uniprot
proteins
mass_dalton
56225
uniprot
proteins
mutagenesis
MUTAGEN 65; /note="Q->E: Impaired 1-alpha hydroxylase activity toward 25-hydroxyvitamin D3. Impaired heme binding."; /evidence="ECO:0000269|PubMed:15972816"; MUTAGEN 408; /note="S->A,V,I: Impaired 1-alpha hydroxylase activity toward 25-hydroxyvitamin D3."; /evidence="ECO:0000269|PubMed:15972816
uniprot
proteins
pathway_participation
PATHWAY: Hormone biosynthesis; vitamin D biosynthesis. {ECO:0000250|UniProtKB:O15528}.
uniprot
proteins
protein_family
Cytochrome P450 family
uniprot
proteins
pubmed
10092858
uniprot
proteins
pubmed
11149489
uniprot
proteins
pubmed
15972816
uniprot
proteins
pubmed
9295274
uniprot
proteins
sequence_length
507
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion membrane.
uniprot
proteins