Amfr
entrez
23802
canonical
uniprot
Q9R049
canonical
ensembl
ENSMUSG00000031751
resolver
ensp
ENSMUSP00000052258
resolver
ensp
ENSMUSP00000160901
resolver
ensp
ENSMUSP00000160909
resolver
genesymbol
Amfr
resolver
uniprot
Q3TCI2
resolver
uniprot
Q8K008
resolver
uniprot
Q99LH5
resolver
uniprot_entry
AMFR_MOUSE
resolver
uniprot_entry
Q3TCI2_MOUSE
resolver
uniprot_sec
Q8K008
resolver
uniprot_sec
Q99LH5
resolver
amino_acid_sequence
MPLLFLERFPWPSLRTYTGLSGLALLGTIVSAYRALSQPEDGSGEPEPLTAPLQPEALAPARLTAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLIAMLLSCCGLAVVCCVTGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELALLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADGSRAREDHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQMTRSVEITTDNILEGRIQVPFPTQRSDSLRPALNSPVERPSPDLEEGEASVQTERVPLDLSPRLEETLDFSEVELEPIEVEDFEARGSRFSKSADERQRMLVQRKDDLLQQARKRFLNKSSEDDGASERLLPSEGTSSDPVTLRRRMLAAAAERRLQRQRTT
uniprot
proteins
ec_number
2.3.2.36
uniprot
proteins
function
FUNCTION: E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation (PubMed:16987818, PubMed:18216283). Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD) (PubMed:16987818, PubMed:18216283). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane (PubMed:22863805). In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination (By similarity). The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction (By similarity). In addition to ubiquitination on lysine residues, catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2/ACAT2 at 'Cys-277', leading to its degradation when the lipid levels are low (By similarity). Catalyzes ubiquitination and subsequent degradation of INSIG1 when cells are depleted of sterols (By similarity). Mediates polyubiquitination of INSIG2 at 'Cys-215' in some tissues, leading to its degradation (By similarity). Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex (By similarity). Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation (By similarity). Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor (PubMed:12650607). In association with LMBR1L and UBAC2, negatively regulates the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 (PubMed:31073040). Regulates NF-kappa-B and MAPK signaling pathways by mediating 'Lys-27'-linked polyubiquitination of TAB3 and promoting subsequent TAK1/MAP3K7 activation (By similarity). {ECO:0000250|UniProtKB:Q9UKV5, ECO:0000269|PubMed:12650607, ECO:0000269|PubMed:16987818, ECO:0000269|PubMed:18216283, ECO:0000269|PubMed:22863805, ECO:0000269|PubMed:31073040}.
uniprot
proteins
mass_dalton
73105
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination.
uniprot
proteins
post_translational_modification
PTM: Palmitoylation of the RING-type zing finger by ZDHHC6 promotes localization to the peripheral endoplasmic reticulum. {ECO:0000250|UniProtKB:Q9UKV5}.
uniprot
proteins
pubmed
10456327
uniprot
proteins
pubmed
12650607
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16709668
uniprot
proteins
pubmed
16987818
uniprot
proteins
pubmed
17114649
uniprot
proteins
pubmed
18216283
uniprot
proteins
pubmed
19144319
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22863805
uniprot
proteins
pubmed
31073040
uniprot
proteins
sequence_length
643
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:16987818}; Multi-pass membrane protein {ECO:0000255}. Note=Palmitoylation promotes localization to the peripheral endoplasmic reticulum. {ECO:0000250|UniProtKB:Q9UKV5}.
uniprot
proteins
transmembrane_region
TRANSMEM 82..102; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 122..142; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 186..206; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 215..235; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 254..274; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 276..296; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 429..449; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins