Pld4
entrez
104759
canonical
uniprot
Q8BG07
canonical
ensembl
ENSMUSG00000052160
resolver
ensp
ENSMUSP00000067002
resolver
ensp
ENSMUSP00000232671
resolver
genesymbol
Pld4
resolver
uniprot
Q3TD59
resolver
uniprot
Q3UA19
resolver
uniprot
Q6PDR0
resolver
uniprot
Q8BR69
resolver
uniprot_entry
PLD4_MOUSE
resolver
uniprot_sec
Q3TD59
resolver
uniprot_sec
Q3UA19
resolver
uniprot_sec
Q6PDR0
resolver
uniprot_sec
Q8BR69
resolver
activity_regulation
ACTIVITY REGULATION: The exonuclease activity toward ssDNA substrate is Ca(2+) and Mg(2+)-independent, but it is inhibited by Fe(2+), Cu(2+) and to a lesser extent Zn(2+) ions. {ECO:0000269|PubMed:38537643}.
uniprot
proteins
amino_acid_sequence
MDKKKEHPEMRIPLQTAVEVSDWPCSTSHDPHSGLGMVLGMLAVLGLSSVTLILFLWQGATSFTSHRMFPEEVPSWSWETLKGDAEQQNNSCQLILVESIPEDLPFAAGSPTAQPLAQAWLQLLDTARESVHIASYYWSLTGLDIGVNDSSSRQGEALLQKFQQLLLRNISVVVATHSPTLAKTSTDLQVLAAHGAQIRQVPMKQLTGGVLHSKFWVVDGRHIYVGSANMDWRSLTQVKELGAIIYNCSNLAQDLEKTFQTYWVLGTPQAVLPKTWPRNFSSHINRFHPLRGPFDGVPTTAYFSASPPSLCPHGRTRDLDAVLGVMEGARQFIYVSVMEYFPTTRFTHHARYWPVLDNALRAAALNKGVHVRLLVSCWFNTDPTMFAYLRSLQAFSNPSAGISVDVKVFIVPVGNHSNIPFSRVNHSKFMVTDKTAYVGTSNWSEDYFSHTAGVGLIVSQKTPRAQPGATTVQEQLRQLFERDWSSHYAMDLDRQVPSQDCVW
uniprot
proteins
ec_number
2.3.1.-
uniprot
proteins
ec_number
3.1.16.1
uniprot
proteins
function
FUNCTION: Lysosomal 5'->3' exonuclease that functions in nucleic-acid sensing by immune cells (PubMed:30111894, PubMed:34620855, PubMed:38537643, PubMed:40931063). Hydrolyzes phosphodiester bond in single-stranded RNA and DNA molecules, with a higher efficiency for molecules with 5' uridine and guanosine residues. It can stall at certain sites and does not necessarily proceed to complete exonucleolytic degradation, thereby producing nucleoside 3'-monophosphates but also 5'-end 5'-hydroxy deoxyribonucleotide/ribonucleotide fragments (PubMed:30111894, PubMed:34620855, PubMed:38537643). Through the processing of self and exogenous RNA and DNA molecules, provides molecular signals to toll-like receptors (TLRs) in innate immunity. Partially redundant with PLD3, it cooperates with the RNase T2/RNASET2 to generate 2',3'-cGMP and cytidine-rich RNA fragments that occupy the two TLR7 ligand-binding pockets, to trigger receptor activation and signaling (By similarity). Can exert polynucleotide phosphatase activity toward 5'-phosphorylated single-stranded DNA substrates although at a slow rate (PubMed:38537643). Could play a role in phagocytosis of activated microglia (PubMed:22102906). {ECO:0000250|UniProtKB:Q96BZ4, ECO:0000269|PubMed:22102906, ECO:0000269|PubMed:30111894, ECO:0000269|PubMed:34620855, ECO:0000269|PubMed:38537643, ECO:0000269|PubMed:40931063}.; FUNCTION: Can alternatively catalyze the transfer (transphosphatidylation) of a glycerol group between (S,R)-lysophosphatidylglycerol (LPG) and monoacylglycerol (MAG). The reaction is characterized by an R-to-S stereo-inversion which is essential for the resistance of the produced (S,S)-bis(monoacylglycero)phosphates (BMPs) to lysosomal degradation. The resulting BMPs, are phospholipids required for the formation of lysosomal intralumenal vesicles (ILVs) where lipid degradation can occur. {ECO:0000269|PubMed:39423811}.
uniprot
proteins
mass_dalton
56154
uniprot
proteins
post_translational_modification
PTM: Highly N-glycosylated. {ECO:0000269|PubMed:21085684, ECO:0000269|PubMed:22102906}.
uniprot
proteins
protein_family
Phospholipase D family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
21085684
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22102906
uniprot
proteins
pubmed
30111894
uniprot
proteins
pubmed
34620855
uniprot
proteins
pubmed
38537643
uniprot
proteins
pubmed
39423811
uniprot
proteins
pubmed
40931063
uniprot
proteins
pubmed
41073790
uniprot
proteins
sequence_length
503
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Lysosome {ECO:0000250|UniProtKB:Q8IV08}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:21085684}; Single-pass type II membrane protein {ECO:0000305|PubMed:21085684}. Golgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:21085684}; Single-pass type II membrane protein {ECO:0000305|PubMed:21085684}. Nucleus {ECO:0000269|PubMed:22102906}. Early endosome {ECO:0000305|PubMed:22102906}. Cytoplasmic vesicle, phagosome {ECO:0000269|PubMed:22102906}. Note=Activation of microglia induces translocation of PLD4 from the nucleus to the phagosomes. {ECO:0000269|PubMed:22102906}.
uniprot
proteins
transmembrane_region
TRANSMEM 37..57; /note="Signal-anchor for type II membrane protein"; /evidence="ECO:0000305
uniprot
proteins