Mdc1
entrez
240087
canonical
uniprot
Q5PSV9
canonical
ensembl
ENSMUSG00000061607
resolver
ensp
ENSMUSP00000080949
resolver
ensp
ENSMUSP00000341348
resolver
ensp
ENSMUSP00000341349
resolver
ensp
ENSMUSP00000341350
resolver
enst
ENSMUST00000082337
resolver
genesymbol
Mdc1
resolver
genesymbol_syn
Kiaa0170
resolver
uniprot
E9QK89
resolver
uniprot
Q52KG1
resolver
uniprot
Q5U4D3
resolver
uniprot
Q6ZQH7
resolver
uniprot_entry
E9QK89_MOUSE
resolver
uniprot_entry
MDC1_MOUSE
resolver
uniprot_sec
Q52KG1
resolver
uniprot_sec
Q5U4D3
resolver
uniprot_sec
Q6ZQH7
resolver
amino_acid_sequence
MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST
uniprot
proteins
function
FUNCTION: Histone reader protein required for checkpoint-mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. Specifically recognizes and binds histone H2AX phosphorylated at 'Ser-139', a marker of DNA damage, serving as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage sites. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53/p53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. Required for chromosomal stability during mitosis by promoting recruitment of TOPBP1 to DNA double strand breaks (DSBs): TOPBP1 forms filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis. Required for the repair of DSBs via homologous recombination by promoting recruitment of NBN component of the MRN complex to DSBs. {ECO:0000250|UniProtKB:Q14676}.
uniprot
proteins
mass_dalton
184670
uniprot
proteins
post_translational_modification
PTM: Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain. Phosphorylated at Ser-168 and Ser-198 by CK2 in response to DNA damage during mitosis, promoting interaction with TOPBP1. Phosphorylated by CK2 in response to DNA damage, promoting interaction with NBN and recruitment of the MRN complex to DNA damage sites. {ECO:0000250|UniProtKB:Q14676}.; PTM: Sumoylation at Lys-1461 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation. {ECO:0000250|UniProtKB:Q14676}.; PTM: Ubiquitinated by RNF4, leading to proteasomal degradation; undergoes 'Lys-48'-linked polyubiquitination. {ECO:0000250|UniProtKB:Q14676}.
uniprot
proteins
pubmed
14621295
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15611643
uniprot
proteins
pubmed
17242355
uniprot
proteins
pubmed
19131326
uniprot
proteins
pubmed
19144319
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
23039116
uniprot
proteins
pubmed
23806337
uniprot
proteins
sequence_length
1707
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q14676}. Chromosome {ECO:0000269|PubMed:15611643}. Note=Associated with chromatin (By similarity). Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AX (By similarity). Colocalizes with APTX at sites of DNA double-strand breaks (By similarity). {ECO:0000250|UniProtKB:Q14676}.
uniprot
proteins