Q60673
uniprot
Q60673
canonical
amino_acid_sequence
MRRPRRPGGSGGSGGSGGLRLLVCLLLLSGRPGGCSAISAHGCLFDRRLCSHLEVCIQDGLFGQCQAGVGQARPLLQVTSPVLQRLQGVLRQLMSQGLSWHDDLTQHVISQEMERIPRLRPPEPHPRDRSGLVPRKPGPAGELLTQGNPTGSSPAAQGFPRPAGGRSWGGSPLSSLQAELLPPLLEHLLMPPQPPHPALTYEPALLQPYLFHQFGSRDGSRGSESSSGVVGVGHLSKAEGPALFSRSASKAILGTHSGHSFGDLTGPSPAQLFQDSGLLYMAQELPVPGRARAPRLPENGGNRAEDSSEGHEEEVLGGRGEKSPPQAAQPELSLQRLTAVLAGYGVELRQLTPEQFSTLLTLLQLLPKGTGRNLEGAVNVGGADVKKTIQQMQRGDPAEALPPTPSLPGYLTASPASSEVQQVLSPGFPEPPHTPSPLGSSSVLLEKKSPLGQSQPTVVGRPSARPSAEEYGYIVTDQKPLSLVAGVRLLEILAEHVHMSSGSFINISVVGPAVTFRIRHNEQNLSLADVTQQAGLVKSELEAQTGLQILQTGVGQREEAAEVLPRQAHGISPMRSVLLTLVALAGVAGLLVALAVALCMRHHSRQRDKERLAALGPEGAHGDTTFEYQDLCRQHMATKSLFNRAEGQPEPSRVSSVSSQFSDAAQASPSSHSSTPSWCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCAAAQDESNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGSSLYHVYEVNLVSEHIWCEDFLVRSFYLKNLQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ
uniprot
proteins
function
FUNCTION: Plays a role in vesicle-mediated secretory processes (PubMed:21732083). Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation (PubMed:15939893, PubMed:21732083). Plays a role in insulin secretion in response to glucose stimuli (PubMed:12031972, PubMed:21732083). Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain (PubMed:16269463). In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (PubMed:16269463). Seems to lack intrinsic enzyme activity (PubMed:7980563, PubMed:8878556). Required to maintain normal levels of renin expression and renin release (PubMed:19019914). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (PubMed:12364328). {ECO:0000250|UniProtKB:Q16849, ECO:0000269|PubMed:12031972, ECO:0000269|PubMed:12364328, ECO:0000269|PubMed:15939893, ECO:0000269|PubMed:19019914, ECO:0000269|PubMed:7980563, ECO:0000269|PubMed:8878556}.; FUNCTION: [ICA512-transmembrane fragment]: ICA512-TMF regulates dynamics and exocytosis of insulin secretory granules (SGs); binding of ICA512-TMF to SNTB2/beta-2-syntrophin is proposed to restrain SGs mobility and exocytosis by tethering them to the actin cytoskeleton depending on UTRN; the function is inhibited by cytoplasmic ICA512-CFF dimerizing with ICA512-TMF and displacing SNTB2 (By similarity). {ECO:0000250|UniProtKB:Q16849}.; FUNCTION: [ICA512-cleaved cytosolic fragment]: ICA512-CCF translocated to the nucleus promotes expression of insulin and other granule-related genes; the function implicates binding to and regulating activity of STAT5B probably by preventing its dephosphorylation and potentially by inducing its sumoylation by recruiting PIAS4 (By similarity). Enhances pancreatic beta-cell proliferation by converging with signaling by STAT5B and STAT3 (PubMed:18178618). ICA512-CCF located in the cytoplasm regulates dynamics and exocytosis of insulin secretory granules (SGs) by dimerizing with ICA512-TMF and displacing SNTB2 thus enhancing SGs mobility and exocytosis (By similarity). {ECO:0000250|UniProtKB:Q16849, ECO:0000269|PubMed:18178618}.
uniprot
proteins
mass_dalton
106083
uniprot
proteins
mutagenesis
MUTAGEN 877; /note="A->D: No effect on dephosphorylating activity; increased the dephosphorylating efficiency; when associated with 911-A."; /evidence="ECO:0000269|PubMed:8878556"; MUTAGEN 911; /note="D->A: Confers dephosphorylating activity; increased the dephosphorylating efficiency; when associated with 877-D."; /evidence="ECO:0000269|PubMed:8878556
uniprot
proteins
post_translational_modification
PTM: Subject to proteolytic cleavage at multiple sites. Subject to cleavage on a pair of basic residues. On exocytosis of secretory granules in pancreatic beta-cells ICA512-TMF is transiently inserted in the plasma-membrane and cleaved by mu-type calpain CPN1 to yield ICA512-CCF. {ECO:0000250|UniProtKB:P56722, ECO:0000250|UniProtKB:Q16849, ECO:0000250|UniProtKB:Q63259}.; PTM: O-glycosylated. {ECO:0000250|UniProtKB:Q16849}.; PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q16849}.; PTM: Sumoylated at two sites including Lys-754. Sumoylation decreases interaction with STAT5. {ECO:0000250|UniProtKB:Q16849}.
uniprot
proteins
protein_family
Protein-tyrosine phosphatase family
uniprot
proteins
protein_family
Receptor class 8 subfamily
uniprot
proteins
pubmed
12031972
uniprot
proteins
pubmed
12364328
uniprot
proteins
pubmed
15939893
uniprot
proteins
pubmed
16269463
uniprot
proteins
pubmed
18178618
uniprot
proteins
pubmed
19019914
uniprot
proteins
pubmed
19361477
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21732083
uniprot
proteins
pubmed
7980563
uniprot
proteins
pubmed
8526904
uniprot
proteins
pubmed
8878556
uniprot
proteins
sequence_length
979
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q63259}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q63259}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000269|PubMed:19361477}; Single-pass type I membrane protein {ECO:0000305}. Perikaryon {ECO:0000250|UniProtKB:Q63259}. Cell projection, axon {ECO:0000250|UniProtKB:Q63259}. Synapse {ECO:0000250|UniProtKB:Q63259}. Cell membrane {ECO:0000250|UniProtKB:Q63259}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q63259}. Endosome {ECO:0000250|UniProtKB:Q63259}. Note=Detected on neuronal secretory vesicles, but not on synaptic vesicles. Colocalizes with insulin-containing secretory granules. Primarily detected on secretory vesicle membranes. Transiently found at the cell membrane, when secretory vesicles fuse with the cell membrane to release their cargo. Is then endocytosed and recycled to secretory vesicles via the Golgi apparatus membranes. {ECO:0000250|UniProtKB:Q63259}.; SUBCELLULAR LOCATION: [ICA512-transmembrane fragment]: Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250|UniProtKB:Q63259}.; SUBCELLULAR LOCATION: [ICA512-cleaved cytosolic fragment]: Nucleus {ECO:0000250|UniProtKB:Q16849}.
uniprot
proteins
transmembrane_region
TRANSMEM 576..600; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins