Ttll8
entrez
239591
canonical
uniprot
A4Q9F1
canonical
ensembl
ENSMUSG00000022388
resolver
ensp
ENSMUSP00000104996
resolver
enst
ENSMUST00000109371
resolver
genesymbol
Ttll8
resolver
uniprot
Q14B76
resolver
uniprot
Q8C0N7
resolver
uniprot
Q8C0V2
resolver
uniprot_entry
Q8C0V2_MOUSE
resolver
uniprot_entry
TTLL8_MOUSE
resolver
uniprot_sec
Q14B76
resolver
uniprot_sec
Q8C0N7
resolver
amino_acid_sequence
MSCPPTPNPPFRPPSHTRVLRTPPLPPWVCLNSKSLSTGVGGQKNQLREASMENGERKKLSSTLSDGDHKEENKLKQGIPQDLSSSPKLDRYKIARQLTEKAIKERKIFSIYGHYPVIRATLRRKGWVEKKFNFFPKALQNLGSEDKSAETKENQEIALERFDDIHDVMSRLVKNEIPYLLWTIKRDVVDYHSLTCDQMLNHYGKTASFTTKIGLCLNMRSLPWYVQANPNTFFPRCYGLCTESEKQEFLDDFRRTVAASILKWVVLHQNYCSKVKGKSKKEEAKNSDPSPKKDPENPDLKLPSLSGQVVDTACKVCQAYLGQLEHEDIDVSEASTEALSEEEWNDLTQQYYLLVHGNASITDSKSYFAQCQALLSKISSVNPQTEIDGIRNIWIIKPAAKSRGRDIVCMDRVENILSLVAADSQTTKDNKWVVQKYIETPMLIYDTKFDIRQWFLVTDWNPLTIWFYKESYLRFSTQRFSLDKLDSAIHLCNNSIQRRLKNDKERSPLLPCHNMWTSTRFQEYLQKRGRGGTWGSIIYPSMKRAVTNAMRVAQDHVEARKNSFELYGADFILGRDFKPWLIEINSSPTMHPSTPVTAQLCAQVQEDTIKVVVDRKLDRNCDIGNFELLWRQPAVELPPFNGSDLCVEGISVKKAKKQMPPIASVGLSESLLDAPPKVRSARALMETVIRPPRTTVRQDWKREEAKVLSTTWSMPVMDAEVRGRAKPIYAFEVNDYQHVDNKSHKSGYTRVQSSKVPGVTLTSAQHPALFAQTMKPTQMTSSPPPTASGNHRDSSPFCPIVFEELWLHPNSQRRPSSCILQSRAQGWIRGIP
uniprot
proteins
ec_number
6.3.2.-
uniprot
proteins
function
FUNCTION: Monoglycylase which modifies both tubulin and non-tubulin proteins, adding a single glycine on the gamma-carboxyl groups of specific glutamate residues to generate monoglycine side chains within the C-terminal tail of target proteins (PubMed:19524510, PubMed:28576883). Not involved in elongation step of the polyglycylation reaction (PubMed:19524510). Preferentially monoglycylates alpha-tubulin over beta-tubulin (PubMed:19524510). Together with TTLL3, mediates microtubule glycylation of primary and motile cilia, which is essential for their stability and maintenance (PubMed:19524510, PubMed:23897886, PubMed:25180231). Together with TTLL3, glycylates sperm flagella which regulates axonemal dynein motor activity, thereby controlling flagellar beat, directional sperm swimming and male fertility (PubMed:33414192). Monoglycylates non-tubulin proteins such as ANP32A, ANP32B, SET, NCL and NAP1 (PubMed:19427864, PubMed:19524510). {ECO:0000269|PubMed:19427864, ECO:0000269|PubMed:19524510, ECO:0000269|PubMed:23897886, ECO:0000269|PubMed:25180231, ECO:0000269|PubMed:28576883, ECO:0000269|PubMed:33414192}.
uniprot
proteins
mass_dalton
94915
uniprot
proteins
mutagenesis
MUTAGEN 93; /note="K->E: Decreased monoglycylation activity; when associated with E-96."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 96; /note="R->E: Decreased monoglycylation activity; when associated with E-93."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 104; /note="K->E: Decreased monoglycylation activity; when associated with E-106."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 106; /note="R->E: Decreased monoglycylation activity; when associated with E-104."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 185; /note="K->E: Decreased monoglycylation activity."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 192; /note="H->E: Decreased monoglycylation activity."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 205; /note="K->E: Decreased monoglycylation activity."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 224; /note="W->A: Decreased monoglycylation activity."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 225; /note="Y->A: Decreased monoglycylation activity."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 328; /note="D->N: Decreased monoglycylation activity; when associated with N-330."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 330; /note="D->N: Decreased monoglycylation activity; when associated with N-328."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 619; /note="R->E: Decreased monoglycylation activity."; /evidence="ECO:0000269|PubMed:28576883"; MUTAGEN 631; /note="R->E: Decreased monoglycylation activity."; /evidence="ECO:0000269|PubMed:28576883
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17499049
uniprot
proteins
pubmed
19427864
uniprot
proteins
pubmed
19524510
uniprot
proteins
pubmed
23897886
uniprot
proteins
pubmed
25180231
uniprot
proteins
pubmed
28576883
uniprot
proteins
pubmed
33414192
uniprot
proteins
sequence_length
832
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000269|PubMed:19427864, ECO:0000269|PubMed:19524510, ECO:0000269|PubMed:33414192}. Cell projection, cilium {ECO:0000305|PubMed:19524510}. Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000305|PubMed:19524510}. Cytoplasm, cytoskeleton, flagellum axoneme {ECO:0000269|PubMed:33414192}.
uniprot
proteins