A6NNM8
uniprot
A6NNM8
canonical
amino_acid_sequence
MEPSTCRTMESEEDYVEEKESEKCVKEGVTNPSNSSQQALLKADYKALKNGVPSPIMATKIPKKVIAPVDTGDLEAGRRKRRRKRRSLAINLTNCKYESVRRAAQMCGLKEVGEDEEWTLYWTDCAVSLERVMDMKRFQKINHFPGMTEICRKDLLARNLNRMYKLYPSEYNIFPRTWCLPADYGDFQSYGRQRKARTYICKPDSGCQGRGIFITRNPREIKPGEHMICQQYISKPLLIDGFKFDMRVYVLITSCDPLRIFTYEEGLARFATTPYMEPSHNNLDNVCMHLTNYAINKHNENFVRDGAVGSKRKLSTLNIWLQEHSYNPGELWGDIEDIIIKTIISAHSVLRHNYRTCFPQYLNGGTCACFEILGFDILLDHKLKPWLLEVNHSPSFTTDSCLDQEVKDALLCDAMTLVNLRGCDKRKVMEEDKRRVKERLFQCYRQPRESRKEKTESSHVAMLDQERYEDSHLGKYRRIYPGPDTEKYARFFKHNGSLFQETAASKAREECARQQLEEIRLKQEQQETSGTKRQKARDQNQGESAGEKSRPRAGLQSLSTHLAYRNRNWEKELLPGQLDTMRPQEIVEEEELERMKALLQRETLIRSLGIVEQLTRLQHPGPQGQKKLHESRDRLGSQELKSMSLVLLVLLRGAATEQGAPHFLHPVLPHESIPRILGALPSMNAAIPHVPRYHLQPKNFNWTGEPAAINSCSLSMKKAGRCYFSSARIRLTSQGQASRRLEAINRVLAGSVPPTLTPKQGYFLQPERVASDSWTECTLPSMVNSEHRAAKVPLCPASAPMLQRSRALLNINQFR
uniprot
proteins
ec_number
6.3.2.-
uniprot
proteins
function
FUNCTION: Polyglutamylase which modifies tubulin, generating polyglutamate side chains of variable lengths on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin (PubMed:40593606). Mediates ATP-dependent polyglutamate side-chain elongation of the polyglutamylation reaction but not the initiation step (By similarity). Preferentially modifies the alpha-tubulin tail over a beta-tail (By similarity). Mediates spindle-pole microtubule glutamylation in a CFAP100-dependent manner, a modification necessary for maintaining spindle-pole integrity and ensuring proper spindle assembly (PubMed:40593606). {ECO:0000250|UniProtKB:A4Q9F6, ECO:0000269|PubMed:40593606}.
uniprot
proteins
mass_dalton
93645
uniprot
proteins
mutagenesis
MUTAGEN 425..427; /note="KRK->EEE: Decreased binding to microtubules and polyglutamylase activity; when associated with 451-E-E-452."; /evidence="ECO:0000269|PubMed:25959773"; MUTAGEN 451..452; /note="RK->EE: Decreased binding to microtubules and polyglutamylase activity; when associated with 425-E--E-427."; /evidence="ECO:0000269|PubMed:25959773
uniprot
proteins
protein_family
Tubulin--tyrosine ligase family
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16572171
uniprot
proteins
pubmed
25959773
uniprot
proteins
pubmed
40593606
uniprot
proteins
sequence_length
815
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:40593606}. Note=Localizes to the spindle pole in a CFAP100-dependent manner. {ECO:0000269|PubMed:40593606}.
uniprot
proteins