Nova1
entrez
664883
canonical
uniprot
Q9JKN6
canonical
ensembl
ENSMUSG00000021047
resolver
ensp
ENSMUSP00000021438
resolver
ensp
ENSMUSP00000189153
resolver
ensp
ENSMUSP00000189155
resolver
ensp
ENSMUSP00000189156
resolver
enst
ENSMUST00000021438
resolver
genesymbol
Nova1
resolver
uniprot
Q8C8B9
resolver
uniprot_entry
NOVA1_MOUSE
resolver
uniprot_sec
Q8C8B9
resolver
amino_acid_sequence
MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTIEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQTLPSSPTTTKSSPSDPMTTSRANQVKIIVPNSTAGLIIGKGGATVKAIMEQSGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGSPYANTAEVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG
uniprot
proteins
function
FUNCTION: RNA-binding protein which regulates alternative splicing of pre-mRNAs in the brain and spinal cord in a sequence-specific manner (PubMed:10719891, PubMed:14615540, PubMed:15933722, PubMed:17065982, PubMed:8558240, PubMed:9154818). Binds to 5'-YCAY-3' repeats, with a minimum of 2 to 3 repeats necessary for high-affinity binding. Mediates both exon inclusion and exclusion, depending upon the position of its binding site within the pre-mRNA (PubMed:10719891, PubMed:15933722, PubMed:9154818). Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly. Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 small nuclear ribonucleoprotein (snRNP) binding and inhibiting exon inclusion, whereas binding to an intronic 5'-YCAY-3' repeat enhances spliceosome assembly and favors exon inclusion (PubMed:17065982). Regulates the splicing of gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2) and glycine receptor subunit alpha-2 (GLRA2) pre-mRNAs, among others (PubMed:10719891, PubMed:12808107, PubMed:15933722, PubMed:9154818). Autoregulates its own splicing. Binds to its own exon 4 and directs its exclusion, thus leading to NOVA1 isoform 2 production (PubMed:15933722, PubMed:9154818). {ECO:0000269|PubMed:10719891, ECO:0000269|PubMed:12808107, ECO:0000269|PubMed:14615540, ECO:0000269|PubMed:15933722, ECO:0000269|PubMed:17065982, ECO:0000269|PubMed:8558240, ECO:0000269|PubMed:9154818}.; FUNCTION: [Isoform 2]: Splicing regulation activity of isoform 2 is similar to that of isoform 1, when tested with minigenes for autosplicing, as well as for GABRG2 and GLRA2 splicing in various cell lines. {ECO:0000269|PubMed:15933722}.
uniprot
proteins
mass_dalton
51756
uniprot
proteins
mutagenesis
MUTAGEN 65; /note="I->T: Markedly decreased RNA binding."; /evidence="ECO:0000269|PubMed:8558240"; MUTAGEN 154; /note="S->A: Strong reduced phosphorylation. Marked reduction in phosphorylation; when associated with A-157. Marked reduction in phosphorylation; when associated with A-161 and A-168. Marked reduction in phosphorylation; when associated with A-157, A-161 and A-168."; /evidence="ECO:0000269|PubMed:15933722"; MUTAGEN 157; /note="T->A: Marked reduction in phosphorylation; when associated with A-154. Marked reduction in phosphorylation; when associated with A-154, A-161 and A-168."; /evidence="ECO:0000269|PubMed:15933722"; MUTAGEN 161; /note="S->A: No effect on phosphorylation; when associated with A-168. Marked reduction in phosphorylation; when associated with A-154 and A-168. Marked reduction in phosphorylation; when associated with A-154, A-157 and A-168."; /evidence="ECO:0000269|PubMed:15933722"; MUTAGEN 168; /note="T->A: No effect on phosphorylation; when associated with A-161. Marked reduction in phosphorylation; when associated with A-154 and A-161. Marked reduction in phosphorylation; when associated with A-154, A-157 and A-161."; /evidence="ECO:0000269|PubMed:15933722"; MUTAGEN 197; /note="I->V: Minimal impact on RNA binding capacity, but leads to specific effects on alternative splicing. When tested in knockin mice, does not affect development, brain size, nor fertility and does not alter motor performance, but affects vocalization patterns in pups and adults."; /evidence="ECO:0000269|PubMed:39966351"; MUTAGEN 444; /note="L->N: Markedly decreased RNA binding."; /evidence="ECO:0000269|PubMed:8558240
uniprot
proteins
post_translational_modification
PTM: [Isoform 2]: Compared to isoform 1, isoform 2 is hardly phosphorylated. {ECO:0000269|PubMed:15933722}.
uniprot
proteins
pubmed
10719891
uniprot
proteins
pubmed
10829067
uniprot
proteins
pubmed
11003693
uniprot
proteins
pubmed
12808107
uniprot
proteins
pubmed
14615540
uniprot
proteins
pubmed
15933722
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17065982
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
30638744
uniprot
proteins
pubmed
39966351
uniprot
proteins
pubmed
8558240
uniprot
proteins
pubmed
9154818
uniprot
proteins
sequence_length
507
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10829067, ECO:0000269|PubMed:8558240}.
uniprot
proteins