Q99P39
uniprot
Q99P39
canonical
activity_regulation
ACTIVITY REGULATION: Active only in complex with LYRM4. {ECO:0000250|UniProtKB:Q9Y697}.
uniprot
proteins
amino_acid_sequence
MLLRVAWRRASLAATSVALRRSSVPTRGLRLRVVDHAPHSAVPSEAEAVLRPLYMDVRATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKLYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQKIMKNLPDVVMNGDPKQHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVQKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH
uniprot
proteins
ec_number
2.8.1.7
uniprot
proteins
function
FUNCTION: [Isoform Mitochondrial]: Cysteine desulfurase, of the core iron-sulfur cluster (ISC) assembly complex, that catalyzes the desulfuration of L-cysteine to L-alanine, as component of the cysteine desulfurase complex leading to the formation of a cysteine persulfide intermediate at the active site cysteine residue and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU. The persulfide is then transferred on the flexible Cys loop from the catalytic site of NFS1 to the surface of NFS1 (By similarity). After the NFS1-linked persulfide sulfur is transferred to one of the conserved Cys residues of the scaffold, a reaction assisted by FXN (By similarity). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity). {ECO:0000250|UniProtKB:Q9H1K1, ECO:0000250|UniProtKB:Q9Y697, ECO:0000250|UniProtKB:Q9Z1J3}.; FUNCTION: [Isoform Cytosolic]: May catalyze the desulfuration of L-cysteine to L-alanine as component of the cysteine desulfurase complex (NFS1:LYRM4), leading to the formation of a cysteine persulfide intermediate. Acts as a sulfur donor for MOCS3 by transferring the sulfur of the cysteine persulfide intermediate on MOCS3. {ECO:0000250|UniProtKB:Q9Y697}.
uniprot
proteins
mass_dalton
50013
uniprot
proteins
post_translational_modification
PTM: N-gluconoylated. {ECO:0000250|UniProtKB:Q9Z1J3}.; PTM: Cysteine persulfide intermediate is reduced by thiol-containing molecules like glutathione and L-cysteine. Persulfide reduction is a rate-limiting step of cysteine desulfurase catalytic cycle. {ECO:0000250|UniProtKB:Q9Z1J3}.
uniprot
proteins
protein_family
Class-V pyridoxal-phosphate-dependent aminotransferase family
uniprot
proteins
protein_family
NifS/IscS subfamily
uniprot
proteins
sequence_length
451
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: [Isoform Mitochondrial]: Mitochondrion {ECO:0000250|UniProtKB:Q9Y697}.; SUBCELLULAR LOCATION: [Isoform Cytosolic]: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9Y697}. Nucleus {ECO:0000250|UniProtKB:Q9Y697}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q9Y697}.
uniprot
proteins