TSG101
entrez
7251
canonical
uniprot
Q99816
canonical
ensembl
ENSG00000074319
resolver
ensp
ENSP00000251968
resolver
genesymbol
TSG101
resolver
hgnc
HGNC:15971
resolver
uniprot
Q9BUM5
resolver
uniprot_entry
TS101_HUMAN
resolver
uniprot_sec
Q9BUM5
resolver
amino_acid_sequence
MAVSESQLKKMVSKYKYRDLTVRETVNVITLYKDLKPVLDSYVFNDGSSRELMNLTGTIPVPYRGNTYNIPICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHEWKHPQSDLLGLIQVMIVVFGDEPPVFSRPISASYPPYQATGPPNTSYMPGMPGGISPYPSGYPPNPSGYPGCPYPPGGPYPATTSSQYPSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY
uniprot
proteins
function
FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426). {ECO:0000269|PubMed:11916981, ECO:0000269|PubMed:17556548, ECO:0000269|PubMed:17853893, ECO:0000269|PubMed:21070952, ECO:0000269|PubMed:21757351, ECO:0000269|PubMed:22315426, ECO:0000269|PubMed:22660413}.
uniprot
proteins
mass_dalton
43944
uniprot
proteins
mutagenesis
MUTAGEN 43; /note="V->A: Reduces interaction with ubiquitin; inhibits down-regulation of EGFR."; /evidence="ECO:0000269|PubMed:12006492"; MUTAGEN 45; /note="N->A: Reduces interaction with ubiquitin. No effect on MGRN1-binding."; /evidence="ECO:0000269|PubMed:12006492, ECO:0000269|PubMed:12802020, ECO:0000269|PubMed:17229889"; MUTAGEN 46; /note="D->A: Reduces interaction with ubiquitin."; /evidence="ECO:0000269|PubMed:12006492"; MUTAGEN 63; /note="Y->A: Reduces interaction with HIV-1 p6; impairs HIV-1 budding."; /evidence="ECO:0000269|PubMed:12006492"; MUTAGEN 88; /note="F->A: Reduces interaction with ubiquitin; no effect on in interaction with HIV-1 p6."; /evidence="ECO:0000269|PubMed:12006492"; MUTAGEN 89; /note="V->A: No change in interaction with p6; no effect on HIV-1 budding."; /evidence="ECO:0000269|PubMed:12006492"; MUTAGEN 95; /note="M->A: Reduces interaction with VPS37B and HIV-1 p6; abolishes interaction with PDCD6IP; impairs HIV-1 budding; inhibits down-regulation of EGFR. Abolishes MGRN1-binding. Loss of interaction with ARRDC1."; /evidence="ECO:0000269|PubMed:12006492, ECO:0000269|PubMed:12802020, ECO:0000269|PubMed:14505570, ECO:0000269|PubMed:15218037, ECO:0000269|PubMed:17229889, ECO:0000269|PubMed:22315426"; MUTAGEN 141; /note="V->A: Reduces interaction with HIV-1 p6."; /evidence="ECO:0000269|PubMed:12006492"; MUTAGEN 158..162; /note="Missing: Abolishes interaction with CEP55 and midbody localization; no effect on interaction with ESCRT-I proteins, PDCD6IP and viral proteins."; /evidence="ECO:0000269|PubMed:17556548"; MUTAGEN 158..160; /note="PPN->AAA: Abolishes interaction with CEP55."; /evidence="ECO:0000269|PubMed:17853893"; MUTAGEN 368..371; /note="RKQF->AAAA: Loss of interaction with VPS28. No effect on interaction with VPS37C."; /evidence="ECO:0000269|PubMed:15509564
uniprot
proteins
post_translational_modification
PTM: Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1. {ECO:0000269|PubMed:15256501, ECO:0000269|PubMed:17229889, ECO:0000269|PubMed:19703557}.
uniprot
proteins
protein_family
UEV subfamily
uniprot
proteins
protein_family
Ubiquitin-conjugating enzyme family
uniprot
proteins
pubmed
10888872
uniprot
proteins
pubmed
11427703
uniprot
proteins
pubmed
11595185
uniprot
proteins
pubmed
11916981
uniprot
proteins
pubmed
12006492
uniprot
proteins
pubmed
12379843
uniprot
proteins
pubmed
12559917
uniprot
proteins
pubmed
12802020
uniprot
proteins
pubmed
12900394
uniprot
proteins
pubmed
14505570
uniprot
proteins
pubmed
14519844
uniprot
proteins
pubmed
14581525
uniprot
proteins
pubmed
15039775
uniprot
proteins
pubmed
15053872
uniprot
proteins
pubmed
15143060
uniprot
proteins
pubmed
15218037
uniprot
proteins
pubmed
15256501
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15509564
uniprot
proteins
pubmed
15858022
uniprot
proteins
pubmed
16552148
uniprot
proteins
pubmed
16571837
uniprot
proteins
pubmed
17229889
uniprot
proteins
pubmed
17556548
uniprot
proteins
pubmed
17853893
uniprot
proteins
pubmed
18005716
uniprot
proteins
pubmed
18256029
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19520058
uniprot
proteins
pubmed
19703557
uniprot
proteins
pubmed
21068219
uniprot
proteins
pubmed
21070952
uniprot
proteins
pubmed
21191027
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21643473
uniprot
proteins
pubmed
21757351
uniprot
proteins
pubmed
22315426
uniprot
proteins
pubmed
22660413
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
23166352
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
25944712
uniprot
proteins
pubmed
9019400
uniprot
proteins
pubmed
9242438
uniprot
proteins
pubmed
9366528
uniprot
proteins
pubmed
9840940
uniprot
proteins
pubmed
9867424
uniprot
proteins
sequence_length
390
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17853893}. Early endosome membrane {ECO:0000269|PubMed:23166352}; Peripheral membrane protein {ECO:0000305|PubMed:23166352}; Cytoplasmic side {ECO:0000305|PubMed:23166352}. Late endosome membrane {ECO:0000269|PubMed:11916981, ECO:0000269|PubMed:17229889}; Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:17853893}. Midbody, Midbody ring {ECO:0000269|PubMed:17556548, ECO:0000269|PubMed:17853893}. Nucleus {ECO:0000269|PubMed:17229889}. Note=Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis. {ECO:0000269|PubMed:17556548, ECO:0000269|PubMed:17853893}.
uniprot
proteins