P24470
uniprot
P24470
canonical
amino_acid_sequence
MELLGFTTLALVVSVTCLSLLSVWTKLRTRGRLPPGPTPLPIIGNLLQLNLKDIPASLSKLAKEYGPVYTLYFGTSPTVVLHGYDVVKEALLQQGDEFLGRGPLPIIEDTHKGYGLIFSNGERWKVMRRFSLMTLRNFGMGKRSLEERVQEEARCLVEELQKTKAQPFDPTFILACAPCNVICSILFNDRFQYNDKTFLNLMDLLNKNFQQVNSVWCQMYNLWPTIIKYLPGKHIEFAKRIDDVKNFILEKVKEHQKSLDPANPRDYIDCFLSKIEEEKDNLKSEFHLENLAVCGSNLFTAGTETTSTTLRFGLLLLMKYPEVQAKVHEELDRVIGRHQPPSMKDKMKLPYTDAVLHEIQRYITLLPSSLPHAVVQDTKFRDYVIPKGTTVLPMLSSVMLDQKEFANPEKFDPGHFLDKNGCFKKTDYFVPFSLGKRACVGESLARMELFLFFTTLLQKFSLKTLVEPKDLDIKPITTGIINLPPPYKLCLVPR
uniprot
proteins
ec_number
1.14.14.-
uniprot
proteins
function
FUNCTION: A cytochrome P450 monooxygenase involved in polyunsaturated fatty acids (PUFAs) metabolism and signaling. Catalyzes preferentially the epoxidation of double bonds of PUFAs. Converts arachidonic acid (ARA, C20:4(n-6)) primarily to stereospecific products 8R,9S-, 11R,12S-, and 14S,15R-EET (PubMed:10491410, PubMed:14742258, PubMed:8246128). Plays a major role in the formation of EETs and hydroxy-EETs (HEETs) in kidney (PubMed:10491410, PubMed:14742258). Via EETs may inhibit the epithelial sodium channels (ENaCs) in nephron segments, preventing excessive sodium absorption during high dietary salt intake (By similarity). Participates in the formation of anti-inflammatory hydroxyepoxyeicosatrienoic acids (HEETs) by converting 20-hydroxyeicosatetraenoic acid (20-HETE) to 20,8,9-HEET, an activator of PPARA (PubMed:14742258). Metabolizes eicosapentaenoic acid (EPA, C20:5(n-3)) to epoxyeicosatetraenoic acid (EETeTr) regioisomers, 8,9-, 11,12-, 14,15-, and 17,18-EETeTr, preferentially producing 17R,18S enantiomer (PubMed:15766564). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:8246128). {ECO:0000250|UniProtKB:E9Q5K4, ECO:0000269|PubMed:10491410, ECO:0000269|PubMed:14742258, ECO:0000269|PubMed:15766564, ECO:0000269|PubMed:8246128}.
uniprot
proteins
mass_dalton
56433
uniprot
proteins
pathway_participation
PATHWAY: Lipid metabolism; arachidonate metabolism. {ECO:0000269|PubMed:10491410, ECO:0000305|PubMed:8246128}.
uniprot
proteins
protein_family
Cytochrome P450 family
uniprot
proteins
pubmed
10491410
uniprot
proteins
pubmed
14742258
uniprot
proteins
pubmed
15766564
uniprot
proteins
pubmed
2263487
uniprot
proteins
pubmed
8246128
uniprot
proteins
pubmed
8514789
uniprot
proteins
sequence_length
494
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:14742258}. Microsome membrane {ECO:0000269|PubMed:14742258}.
uniprot
proteins