Spast
entrez
50850
canonical
uniprot
Q9QYY8
canonical
ensembl
ENSMUSG00000024068
resolver
ensp
ENSMUSP00000024869
resolver
ensp
ENSMUSP00000153469
resolver
ensp
ENSMUSP00000156393
resolver
ensp
ENSMUSP00000322193
resolver
ensp
ENSMUSP00000322204
resolver
enst
ENSMUST00000224711
resolver
enst
ENSMUST00000233611
resolver
genesymbol
Spast
resolver
genesymbol_syn
Kiaa1083
resolver
genesymbol_syn
SPAST
resolver
genesymbol_syn
SPG4
resolver
genesymbol_syn
Spg4
resolver
uniprot
A0A286YE25
resolver
uniprot
A0A3B2WC92
resolver
uniprot
Q6ZPY6
resolver
uniprot
Q80VE0
resolver
uniprot
Q9CVK0
resolver
uniprot_entry
A0A286YE25_MOUSE
resolver
uniprot_entry
A0A3B2WC92_MOUSE
resolver
uniprot_entry
SPAST_MOUSE
resolver
uniprot_sec
Q6ZPY6
resolver
uniprot_sec
Q80VE0
resolver
uniprot_sec
Q9CVK0
resolver
activity_regulation
ACTIVITY REGULATION: Allosteric enzyme with a cooperative mechanism; at least two neighbor subunits influence each other strongly in spastin hexamers. Microtubule binding promotes cooperative interactions among spastin subunits. {ECO:0000255|HAMAP-Rule:MF_03021}.
uniprot
proteins
amino_acid_sequence
MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLGLLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFSKSQTDVYNESTNLTCRNGHLQSESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV
uniprot
proteins
ec_number
5.6.1.1
uniprot
proteins
function
FUNCTION: ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:19141076, PubMed:20530212). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (By similarity). Severing activity is not dependent on tubulin acetylation or detyrosination (By similarity). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation (By similarity). It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia (By similarity). SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (By similarity). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (By similarity). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (By similarity). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (By similarity). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (By similarity). Probably plays a role in axon growth and the formation of axonal branches (PubMed:18234839). {ECO:0000250|UniProtKB:Q9UBP0, ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:18234839, ECO:0000269|PubMed:19141076, ECO:0000269|PubMed:20530212}.; FUNCTION: [Isoform 1]: Involved in lipid metabolism by regulating the size and distribution of lipid droplets. {ECO:0000250|UniProtKB:Q9UBP0}.
uniprot
proteins
mass_dalton
66456
uniprot
proteins
mutagenesis
MUTAGEN 360; /note="S->C: Decreased microtubule severing activity."; /evidence="ECO:0000269|PubMed:20530212"; MUTAGEN 553; /note="D->A: Decreased microtubule severing activity."; /evidence="ECO:0000269|PubMed:20530212
uniprot
proteins
protein_family
AAA ATPase family
uniprot
proteins
protein_family
Spastin subfamily
uniprot
proteins
pubmed
10610178
uniprot
proteins
pubmed
14621295
uniprot
proteins
pubmed
15345747
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16026783
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17101632
uniprot
proteins
pubmed
18234839
uniprot
proteins
pubmed
18495362
uniprot
proteins
pubmed
19141076
uniprot
proteins
pubmed
19453301
uniprot
proteins
pubmed
20530212
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22773755
uniprot
proteins
sequence_length
614
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Membrane {ECO:0000255|HAMAP-Rule:MF_03021}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03021}. Endoplasmic reticulum {ECO:0000255|HAMAP-Rule:MF_03021}. Midbody {ECO:0000255|HAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000255|HAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-Rule:MF_03021}. Cytoplasm, perinuclear region {ECO:0000255|HAMAP-Rule:MF_03021}. Nucleus {ECO:0000255|HAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton, spindle {ECO:0000255|HAMAP-Rule:MF_03021}. Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03021}. Cell projection, axon {ECO:0000250|UniProtKB:Q9UBP0}. Note=Forms an intramembrane hairpin-like structure in the membrane. Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B (By similarity). Enriched in the distal axons and branches of postmitotic neurons (By similarity). Evenly distributed along early axons and concentrates in the growth cone of the axons of late stage 3 neurons (PubMed:18234839). Mainly nuclear in interphase cells and becomes associated with the centrosomes, spindle microtubules, midzone and finally the midbody during cell division (By similarity). {ECO:0000250|UniProtKB:Q9UBP0, ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:18234839}.; SUBCELLULAR LOCATION: [Isoform 1]: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9UBP0}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9UBP0}. Nucleus membrane {ECO:0000250|UniProtKB:Q9UBP0}. Lipid droplet {ECO:0000250|UniProtKB:Q9UBP0}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9UBP0}. Endosome {ECO:0000250|UniProtKB:Q9UBP0}. Note=Forms an intramembrane hairpin-like structure in the membrane. Recruited to nuclear membrane by IST1 during late anaphase. Localizes to endoplasmic reticulum tubular network. {ECO:0000250|UniProtKB:Q9UBP0}.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm {ECO:0000250|UniProtKB:Q9UBP0}. Endosome {ECO:0000250|UniProtKB:Q9UBP0}. Nucleus membrane {ECO:0000250|UniProtKB:Q9UBP0}. Note=Constitutes the main endosomal form. Recruited to nuclear membrane by IST1 during late anaphase. {ECO:0000250|UniProtKB:Q9UBP0}.
uniprot
proteins