Ndst1
entrez
29633
canonical
uniprot
Q02353
canonical
ensembl
ENSRNOG00000019014
resolver
ensembl
ENSRNOG00055014950
resolver
ensembl
ENSRNOG00060021258
resolver
ensembl
ENSRNOG00065022860
resolver
ensp
ENSRNOP00000025881
resolver
ensp
ENSRNOP00055020854
resolver
ensp
ENSRNOP00060030321
resolver
ensp
ENSRNOP00065031720
resolver
enst
ENSRNOT00000025881
resolver
enst
ENSRNOT00055025567
resolver
enst
ENSRNOT00060036810
resolver
enst
ENSRNOT00065039064
resolver
genesymbol
Ndst1
resolver
genesymbol_syn
Hsst
resolver
genesymbol_syn
Hsst1
resolver
uniprot_entry
NDST1_RAT
resolver
activity_regulation
ACTIVITY REGULATION: The deacetylase activity is specifically inhibited by N-Ethylmaleimide (PubMed:8483907). The sulfotransferase activity is specifically inhibited by 3',5'-ADP (PubMed:8483907). {ECO:0000269|PubMed:8483907}.
uniprot
proteins
amino_acid_sequence
MPALACLRRLCRHLSPQAVLFLLFVFCLFSVFVSAYYLYGWNRGLEPSADASESDCGDPPPVAPSRLLPIKPVQAVAPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRTHIPNFTFNLGYSGKFFHTGTDAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWNIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPISVFMTHLSNYGNDRLGLYTFKHLVRFLHSWTNLRLQTLPPVQLAQKYFQIFSEEKDPLWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLGMHPDLSSNYPSSETFEEIQFFNGHNYHKGIDWYMEFFPIPSNTTSDFYFEKSANYFDSEVAPRRAAALLPKAKVLTILINPADRAYSWYQHQRAHDDPVALKYTFHEVITAGPDASSKLRALQNRCLVPGWYATHIERWLSAFHANQILVLDGKLLRTEPAKVMDTVQKFLGVTSTVDYHKTLAFDPKKGFWCQLLEGGKTKCLGKSKGRKYPEMDLDSRAFLKDYYRDHNIELSKLLYKMGQTLPTWLREDLQNTR
uniprot
proteins
ec_number
2.8.2.8
uniprot
proteins
ec_number
3.5.1.-
uniprot
proteins
function
FUNCTION: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate (PubMed:1379236, PubMed:3422231, PubMed:8483907, PubMed:9890952). Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:1379236, PubMed:3422231). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity). {ECO:0000250|UniProtKB:P52848, ECO:0000250|UniProtKB:Q3UHN9, ECO:0000269|PubMed:1379236, ECO:0000269|PubMed:3422231, ECO:0000269|PubMed:8483907, ECO:0000269|PubMed:9890952}.
uniprot
proteins
mass_dalton
100785
uniprot
proteins
mutagenesis
MUTAGEN 56; /note="C->A: Induces a reduction in sulfotransferase activity but does not affect deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 159; /note="C->A: Induces a reduction in sulfotransferase activity but increases deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 486; /note="C->A,V: Does not affect sulfotransferase activity but increases deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 486; /note="C->F: Does not affect sulfotransferase activity but strongly decreases deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 486; /note="C->G: Does not affect sulfotransferase activity but weakly affects deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 486; /note="C->R,W: Does not affect sulfotransferase activity but abolishes deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 586; /note="C->A: Loss of deacetylase and sulfotransferase activities."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 601; /note="C->A: Loss of sulfotransferase activity but does not affect deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 751; /note="C->A: Does not affect neither deacetylase nor sulfotransferase activities."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 818; /note="C->A: Loss of sulfotransferase activity but does not affect deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952"; MUTAGEN 828; /note="C->A: Loss of sulfotransferase activity but does not affect deacetylase activity."; /evidence="ECO:0000269|PubMed:9890952
uniprot
proteins
pathway_participation
PATHWAY: Glycan metabolism; heparan sulfate biosynthesis. {ECO:0000269|PubMed:1379236, ECO:0000269|PubMed:3422231}.; PATHWAY: Glycan metabolism; heparin biosynthesis. {ECO:0000305|PubMed:1379236, ECO:0000305|PubMed:3422231}.
uniprot
proteins
protein_family
NDST subfamily
uniprot
proteins
protein_family
Sulfotransferase 1 family
uniprot
proteins
pubmed
11792394
uniprot
proteins
pubmed
1379236
uniprot
proteins
pubmed
3422231
uniprot
proteins
pubmed
8483907
uniprot
proteins
pubmed
9890952
uniprot
proteins
sequence_length
882
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:11792394}; Single-pass type II membrane protein {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:P52848}; Single-pass type II membrane protein {ECO:0000255}. Golgi apparatus, cis-Golgi network membrane {ECO:0000250|UniProtKB:P52848}; Single-pass type II membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 18..39; /note="Helical; Signal-anchor for type II membrane protein"; /evidence="ECO:0000255
uniprot
proteins