Git1
entrez
83709
canonical
uniprot
Q9Z272
canonical
ensembl
ENSRNOG00000061270
resolver
ensembl
ENSRNOG00055026761
resolver
ensembl
ENSRNOG00060029464
resolver
ensembl
ENSRNOG00065019852
resolver
ensp
ENSRNOP00000073267
resolver
ensp
ENSRNOP00000090288
resolver
ensp
ENSRNOP00055038020
resolver
ensp
ENSRNOP00060042604
resolver
ensp
ENSRNOP00065026813
resolver
enst
ENSRNOT00000078730
resolver
enst
ENSRNOT00055046345
resolver
enst
ENSRNOT00060051210
resolver
enst
ENSRNOT00065033521
resolver
genesymbol
Git1
resolver
uniprot_entry
GIT1_RAT
resolver
amino_acid_sequence
MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSARNQSDLDDQHDYDSVASDEDTDQEPLPSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRKLQREIHKLQAENLQLRQPPGPVPVPSLPSERAEHTLMGPGGSTHRRDRQAFSMYEPGSALKPFGGAPGDELATRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASSVTFTPSSPLLSSSQEGSRHASKLSRHGSGAESDYENTQSGEPLLGLEGKRFLELSKEDELHAELESLDGDPDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ
uniprot
proteins
function
FUNCTION: GTPase-activating protein for ADP ribosylation factor family members, including ARF1 (PubMed:9826657). Multidomain scaffold protein that interacts with numerous proteins and therefore participates in many cellular functions, including receptor internalization, focal adhesion remodeling, and signaling by both G protein-coupled receptors and tyrosine kinase receptors (PubMed:9826657). Through PAK1 activation, positively regulates microtubule nucleation during interphase. Plays a role in the regulation of cytokinesis; for this function, may act in a pathway also involving ENTR1 and PTPN13 (By similarity). May promote cell motility both by regulating focal complex dynamics and by the activation of RAC1 (PubMed:10938112). May act as scaffold for MAPK1/3 signal transduction, recruiting MAPK1/3 to focal adhesions after EGF stimulation via a Src-dependent pathway, hence stimulating cell migration (PubMed:15923189). Plays a role in brain development and function. Involved in the regulation of spine density and synaptic plasticity that is required for processes involved in learning (By similarity). Plays an important role in dendritic spine morphogenesis and synapse formation (PubMed:12695502, PubMed:15800193, PubMed:17310244, PubMed:24297929, PubMed:25009255). In hippocampal neurons, recruits guanine nucleotide exchange factors (GEFs), such as ARHGEF7/beta-PIX, to the synaptic membrane. These in turn locally activate RAC1, which is an essential step for spine morphogenesis and synapse formation (PubMed:12473661). May contribute to the organization of presynaptic active zones through oligomerization and formation of a Piccolo/PCLO-based protein network, which includes ARHGEF7/beta-PIX and FAK1 (PubMed:12473661). In neurons, through its interaction with liprin-alpha family members, may be required for AMPA receptor (GRIA2/3) proper targeting to the cell membrane (PubMed:12629171). In complex with GABA(A) receptors and ARHGEF7, plays a crucial role in regulating GABA(A) receptor synaptic stability, maintaining GPHN/gephyrin scaffolds and hence GABAergic inhibitory synaptic transmission, by locally coordinating RAC1 and PAK1 downstream effector activity, leading to F-actin stabilization (PubMed:25284783). May also be important for RAC1 downstream signaling pathway through PAK3 and regulation of neuronal inhibitory transmission at presynaptic input (By similarity). Required for successful bone regeneration during fracture healing. The function in intramembranous ossification may, at least partly, exerted by macrophages in which GIT1 is a key negative regulator of redox homeostasis, IL1B production, and glycolysis, acting through the ERK1/2/NRF2/NFE2L2 axis (By similarity). May also play a role in angiogenesis during fracture healing (By similarity). In this process, may regulate activation of the canonical NF-kappa-B signal in bone mesenchymal stem cells by enhancing the interaction between NEMO and 'Lys-63'-ubiquitinated RIPK1/RIP1, eventually leading to enhanced production of VEGFA and others angiogenic factors (By similarity). Essential for VEGF signaling through the activation of phospholipase C-gamma and ERK1/2, hence may control endothelial cell proliferation and angiogenesis (By similarity). {ECO:0000250|UniProtKB:Q68FF6, ECO:0000250|UniProtKB:Q9Y2X7, ECO:0000269|PubMed:10938112, ECO:0000269|PubMed:12473661, ECO:0000269|PubMed:12629171, ECO:0000269|PubMed:12695502, ECO:0000269|PubMed:15800193, ECO:0000269|PubMed:15923189, ECO:0000269|PubMed:17310244, ECO:0000269|PubMed:24297929, ECO:0000269|PubMed:25009255, ECO:0000269|PubMed:25284783, ECO:0000269|PubMed:9826657}.
uniprot
proteins
mass_dalton
85231
uniprot
proteins
mutagenesis
MUTAGEN 285; /note="F->A: Loss of interaction with FAK1, decreased interaction with PCLO, no effect on interaction with ARHGEF7."; /evidence="ECO:0000269|PubMed:12473661"; MUTAGEN 288; /note="L->A: Loss of interaction with PCLO and FAK1, no effect on interaction with ARHGEF7."; /evidence="ECO:0000269|PubMed:12473661"; MUTAGEN 298; /note="R->A: Loss of interaction with FAK1, decreased interaction with PCLO, no effect on interaction with ARHGEF7."; /evidence="ECO:0000269|PubMed:12473661"; MUTAGEN 299; /note="R->A: Loss of interaction with FAK1, decreased interaction with PCLO, no effect on interaction with ARHGEF7."; /evidence="ECO:0000269|PubMed:12473661"; MUTAGEN 392; /note="Y->F: Loss of interaction with NCK2."; /evidence="ECO:0000269|PubMed:17310244"; MUTAGEN 432..483; /note="Missing: Decreased homooligomerization and loss of formation of a ternary complex between GIT1, ARHGEF7 and PCLO. Does not affect ARHGEF7- and PCLO-binding to GIT1 monomer."; /evidence="ECO:0000269|PubMed:12473661
uniprot
proteins
post_translational_modification
PTM: Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts (PubMed:10938112). Phosphorylation at Tyr-392 is induced by activation of Ephrin-B1/EFNB1 and catalyzed by SRC family kinases. It is required for the interaction with NCK2 and for GIT1 recruitment to synapses in hippocampal neurons (PubMed:17310244). {ECO:0000269|PubMed:10938112, ECO:0000269|PubMed:17310244}.
uniprot
proteins
pubmed
10938112
uniprot
proteins
pubmed
12153727
uniprot
proteins
pubmed
12473661
uniprot
proteins
pubmed
12629171
uniprot
proteins
pubmed
12695502
uniprot
proteins
pubmed
15800193
uniprot
proteins
pubmed
15923189
uniprot
proteins
pubmed
17310244
uniprot
proteins
pubmed
19136011
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
24297929
uniprot
proteins
pubmed
25009255
uniprot
proteins
pubmed
25284783
uniprot
proteins
pubmed
9826657
uniprot
proteins
sequence_length
770
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Y2X7}. Synapse {ECO:0000269|PubMed:17310244, ECO:0000269|PubMed:25284783}. Presynapse {ECO:0000269|PubMed:12473661, ECO:0000269|PubMed:12629171, ECO:0000269|PubMed:12695502}. Postsynapse {ECO:0000269|PubMed:12629171, ECO:0000269|PubMed:12695502}. Postsynaptic density {ECO:0000269|PubMed:12629171}. Cell junction, focal adhesion {ECO:0000305|PubMed:10938112}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:Q9Y2X7}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q9Y2X7}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000250|UniProtKB:Q9Y2X7}. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytosolic complexes, containing at least PXN/paxillin, ARHGEF7 and PAK1, and membrane protrusions. During cell migration, moves from the disassembling adhesions into the cytosol and towards the leading edge. In adherent cells, localizes to adhesions. Recruitment to adhesions may be mediated by RAC1 and active tyrosine-phosphorylated PXN (By similarity). May be present in both excitatory, as well as inhibitory synapses (PubMed:12695502, PubMed:25284783). In hippocampal neurons, recruitment of GIT1 to synapses is regulated by ephrinB activation and ephrinB downstream effector GRB4/NCK2 (PubMed:17310244). In hippocampal neurons, partially colocalizes with PCLO (PubMed:12473661). Interaction with GRIN3A limits GIT1 synaptic localization (PubMed:24297929). Localization to the centrosome does not depend upon the presence of gamma-tubulin (By similarity). {ECO:0000250|UniProtKB:Q9Y2X7, ECO:0000269|PubMed:12473661, ECO:0000269|PubMed:12695502, ECO:0000269|PubMed:17310244, ECO:0000269|PubMed:24297929, ECO:0000269|PubMed:25284783}.
uniprot
proteins