Rtcb
entrez
28088
canonical
uniprot
Q99LF4
canonical
ensembl
ENSMUSG00000001783
resolver
ensp
ENSMUSP00000001834
resolver
enst
ENSMUST00000001834
resolver
genesymbol
Rtcb
resolver
genesymbol_syn
D10Wsu52e
resolver
uniprot
Q3TA04
resolver
uniprot
Q3TI25
resolver
uniprot
Q3UDW6
resolver
uniprot_entry
RTCB_MOUSE
resolver
uniprot_sec
Q3TA04
resolver
uniprot_sec
Q3TI25
resolver
uniprot_sec
Q3UDW6
resolver
activity_regulation
ACTIVITY REGULATION: ZBTB8OS/Archease stimulates the activity of the tRNA ligase complex by participating to RTCB active site guanylylation reaction. While Mg(2+), Ca(2+) and to a lesser extent Co(2+) show low levels of covalent incorporation of GMP the strongest guanylylation occurs in presence of Mn(2+) ions. {ECO:0000250|UniProtKB:Q9Y3I0}.
uniprot
proteins
amino_acid_sequence
MSRNYNDELQFLDKINKNCWRIKKGFVPNMQVEGVFYVNDALEKLMFEELRNACRGGGVGGFLPAMKQIGNVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDPEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVKEQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSPRAKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGISKKAIKLRPIAVIKG
uniprot
proteins
ec_number
6.5.1.8
uniprot
proteins
function
FUNCTION: 3'-5' RNA ligase, catalytic subunit of the tRNA-splicing ligase complex (tRNA-LC), which is involved in the enzyme-dependent maturation of intron-containing pre-tRNAs. Functions downstream of the tRNA-splicing endonuclease that removes introns, ligating the two generated halves via phosphodiester bond formation. The ligation reaction, which requires guanosine triphosphate (GTP) and Mn(2+), proceeds through three metal-dependent steps. The first step requires ZBTB8OS/Archease and involves the guanylylation of RTCB at its active site histidine, forming a covalent GMP-histidine intermediate. Before the second step, RTCB also hydrolyzes the 2',3'-cyclic phosphate (cP) at the 3' end of the 5' tRNA exon, typically generated by the tRNA-splicing endonuclease, producing a 3' phosphate. The covalently bound GMP is then transferred to this 3' phosphate to form an RNA(3')-P-P-(5')G intermediate. In the final step, the 5'-OH of the 3' exon attacks the activated 3' end of the 5' exon, forming a 3'-5' phosphodiester bond and releasing GMP. RTCB also functions in non-canonical, spliceosome-independent, cytoplasmic splicing of XBP1 mRNAs during the unfolded protein response (UPR). Upon endoplasmic reticulum (ER) stress, the endoribonuclease IRE1/ERN1 excises a short intron, generating free exon ends that are aligned by RNA-intrinsic, zipper-like stem-loop structures. These exon ends are then recognized and ligated by RTCB. This splicing event yields the active XBP1s transcription factor, which induces genes required to resolve protein folding defects in the endoplasmic reticulum. {ECO:0000250|UniProtKB:Q9Y3I0}.
uniprot
proteins
mass_dalton
55249
uniprot
proteins
protein_family
RtcB family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
18508721
uniprot
proteins
pubmed
20510672
uniprot
proteins
pubmed
21046479
uniprot
proteins
pubmed
21183079
uniprot
proteins
sequence_length
505
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y3I0}. Cytoplasm {ECO:0000269|PubMed:18508721}. Note=Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low. {ECO:0000250|UniProtKB:Q9Y3I0}.
uniprot
proteins