PUS10
entrez
150962
canonical
uniprot
Q3MIT2
canonical
ensembl
ENSG00000162927
resolver
ensp
ENSP00000326003
resolver
ensp
ENSP00000386074
resolver
ensp
ENSP00000572178
resolver
ensp
ENSP00000572179
resolver
ensp
ENSP00000572182
resolver
ensp
ENSP00000572183
resolver
ensp
ENSP00000572184
resolver
ensp
ENSP00000572185
resolver
ensp
ENSP00000572186
resolver
ensp
ENSP00000572187
resolver
ensp
ENSP00000641290
resolver
ensp
ENSP00000641291
resolver
ensp
ENSP00000641292
resolver
ensp
ENSP00000641293
resolver
ensp
ENSP00000641294
resolver
ensp
ENSP00000641295
resolver
ensp
ENSP00000641296
resolver
enst
ENST00000316752
resolver
enst
ENST00000407787
resolver
enst
ENST00000902119
resolver
enst
ENST00000902123
resolver
enst
ENST00000902124
resolver
enst
ENST00000902125
resolver
enst
ENST00000902126
resolver
enst
ENST00000902127
resolver
enst
ENST00000902128
resolver
enst
ENST00000971231
resolver
enst
ENST00000971232
resolver
enst
ENST00000971233
resolver
genesymbol
PUS10
resolver
genesymbol_syn
CCDC139
resolver
genesymbol_syn
DOBI
resolver
hgnc
HGNC:26505
resolver
uniprot
A8K6R4
resolver
uniprot
Q5JPJ5
resolver
uniprot
Q96MI8
resolver
uniprot_entry
A8K6R4_HUMAN
resolver
uniprot_entry
PUS10_HUMAN
resolver
uniprot_sec
Q5JPJ5
resolver
uniprot_sec
Q96MI8
resolver
amino_acid_sequence
MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD
uniprot
proteins
ec_number
5.4.99.25
uniprot
proteins
function
FUNCTION: Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm (PubMed:31819270, PubMed:33023933). In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs (PubMed:30530625, PubMed:31819270, PubMed:33023933). tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs (PubMed:30530625). Does not show tRNA pseudouridylate synthase activity in the nucleus (PubMed:33023933). In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity (PubMed:31819270). Binds pri-miRNAs (PubMed:31819270). Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage (PubMed:14527409, PubMed:19712588). Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death (PubMed:19712588). {ECO:0000269|PubMed:14527409, ECO:0000269|PubMed:19712588, ECO:0000269|PubMed:30530625, ECO:0000269|PubMed:31819270, ECO:0000269|PubMed:33023933}.
uniprot
proteins
mass_dalton
60244
uniprot
proteins
mutagenesis
MUTAGEN 344; /note="D->A: Abolished tRNA pseudouridine synthase without affecting ability to promote miRNA processing."; /evidence="ECO:0000269|PubMed:30530625, ECO:0000269|PubMed:31819270
uniprot
proteins
post_translational_modification
PTM: Proteolytically cleaved during TRAIL-induced cell death (PubMed:19712588). Cleaved, in vitro, either by caspase-3 (CASP3) or caspase-8 (CASP8) (PubMed:19712588). {ECO:0000269|PubMed:19712588}.
uniprot
proteins
protein_family
Pseudouridine synthase Pus10 family
uniprot
proteins
pubmed
14527409
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
17525332
uniprot
proteins
pubmed
17900615
uniprot
proteins
pubmed
17974005
uniprot
proteins
pubmed
19712588
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
28981101
uniprot
proteins
pubmed
30530625
uniprot
proteins
pubmed
31819270
uniprot
proteins
pubmed
33023933
uniprot
proteins
sequence_length
529
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28981101, ECO:0000269|PubMed:30530625, ECO:0000269|PubMed:31819270, ECO:0000269|PubMed:33023933}. Cytoplasm {ECO:0000269|PubMed:31819270, ECO:0000269|PubMed:33023933, ECO:0000305|PubMed:30530625}. Mitochondrion {ECO:0000269|PubMed:28981101}. Note=Localizes mainly in the nucleus (Probable) (PubMed:31819270). tRNA pseudouridylate synthase activity is restricted to the cytoplasm (PubMed:31819270). Translocates from nucleus to mitochondria during TRAIL-induced apoptosis (PubMed:28981101). {ECO:0000269|PubMed:28981101, ECO:0000269|PubMed:31819270, ECO:0000305|PubMed:30530625}.
uniprot
proteins