ATG12
entrez
9140
canonical
uniprot
O94817
canonical
ensembl
ENSG00000145782
resolver
ensp
ENSP00000425107
resolver
ensp
ENSP00000425164
resolver
genesymbol
ATG12
resolver
genesymbol_syn
APG12
resolver
genesymbol_syn
APG12L
resolver
hgnc
HGNC:588
resolver
uniprot
Q6PJV2
resolver
uniprot_entry
ATG12_HUMAN
resolver
uniprot_sec
Q6PJV2
resolver
amino_acid_sequence
MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG
uniprot
proteins
function
FUNCTION: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). {ECO:0000250|UniProtKB:Q9CQY1, ECO:0000269|PubMed:12207896, ECO:0000269|PubMed:17999726, ECO:0000269|PubMed:19074260, ECO:0000269|PubMed:19164948, ECO:0000269|PubMed:23202584}.; FUNCTION: (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601). {ECO:0000269|PubMed:17709747, ECO:0000269|PubMed:19666601}.
uniprot
proteins
mass_dalton
15113
uniprot
proteins
mutagenesis
MUTAGEN 54; /note="K->D: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 62; /note="V->R: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 63; /note="G->D: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 72; /note="K->D: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 73; /note="W->A: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 108; /note="F->A,D,R: Impairs ATG12 stability."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 113; /note="D->V: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 122; /note="C->W: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 123; /note="F->D: Impairs ATG12 stability."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 138; /note="A->R: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584"; MUTAGEN 139; /note="W->F,Y: Impairs E3 activity of the ATG12-ATG5 conjugate."; /evidence="ECO:0000269|PubMed:23202584
uniprot
proteins
post_translational_modification
PTM: Acetylated by EP300. {ECO:0000269|PubMed:19124466}.
uniprot
proteins
protein_family
ATG12 family
uniprot
proteins
pubmed
11096062
uniprot
proteins
pubmed
11825910
uniprot
proteins
pubmed
12207896
uniprot
proteins
pubmed
15372022
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16963840
uniprot
proteins
pubmed
17457038
uniprot
proteins
pubmed
17709747
uniprot
proteins
pubmed
17999726
uniprot
proteins
pubmed
18704115
uniprot
proteins
pubmed
19074260
uniprot
proteins
pubmed
19124466
uniprot
proteins
pubmed
19164948
uniprot
proteins
pubmed
19473119
uniprot
proteins
pubmed
19666601
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22342342
uniprot
proteins
pubmed
23202584
uniprot
proteins
pubmed
24191030
uniprot
proteins
pubmed
26812546
uniprot
proteins
pubmed
32960676
uniprot
proteins
pubmed
34870550
uniprot
proteins
pubmed
9852036
uniprot
proteins
sequence_length
140
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Preautophagosomal structure membrane {ECO:0000269|PubMed:22342342}; Peripheral membrane protein {ECO:0000269|PubMed:22342342}. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.
uniprot
proteins