Pold3
entrez
67967
canonical
uniprot
Q9EQ28
canonical
ensembl
ENSMUSG00000030726
resolver
ensp
ENSMUSP00000032969
resolver
ensp
ENSMUSP00000146603
resolver
ensp
ENSMUSP00000220873
resolver
enst
ENSMUST00000032969
resolver
enst
ENSMUST00000208670
resolver
genesymbol
Pold3
resolver
uniprot
A0A140LHY7
resolver
uniprot
F8WJ15
resolver
uniprot
Q8BH76
resolver
uniprot_entry
A0A140LHY7_MOUSE
resolver
uniprot_entry
DPOD3_MOUSE
resolver
uniprot_entry
F8WJ15_MOUSE
resolver
uniprot_entry
Q8BH76_MOUSE
resolver
amino_acid_sequence
MAEQLYLENIDEFVTDQNKIVTYKWLSYTLGVHVNQAKQMLYEYVERKRKENSGAQLHVTYLVSGSLIQNGHSCHKVAVVREDKLEAVKSKLAVTASIHVYSIQKAMLKDSGPLFNTDYDILKSNLQNCSKFSAIQCAAAVPRAPAESPSSRKYEQSNLQAASEAQASELTTNGHGPPASKQASQQPKGIMGMLISKAATKTQDTNKETKPEAREVTSASSAGGKAPGKGSVMSNFFGKAAMNKLKVNLDSEQAVKEEKTVEQPPVSVTEPKLAAPPAQKKSSRKSEPGKVQQKEKSSRGKRVDLSDEEAKETEHLKKKRRRIKLPQSDSSEDEVFEDSPEMYEADSPSPPPVSPPPDPMPKTEPPPVKRSSGETKRRRKRVLKSKTFVDEEGCIVTEKVYESESCTDSEEELKMKPASAHKPPAAAVKREPREERKGPKKGAAALGKANRQVSITGFFQKK
uniprot
proteins
function
FUNCTION: Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex (By similarity). As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:10219083, PubMed:27524497). Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the tetrameric DNA polymerase zeta complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the DNA polymerase zeta complex compared to the minimal zeta complex, consisting of only REV3L and REV7 (By similarity). {ECO:0000250|UniProtKB:Q15054, ECO:0000269|PubMed:10219083, ECO:0000269|PubMed:27524497}.
uniprot
proteins
mass_dalton
50836
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated, but not targeted to the proteasome. Sumoylated. Sumoylation by SUMO3 may be predominant. {ECO:0000250|UniProtKB:Q15054}.; PTM: Phosphorylation at Ser-454 is thought to decrease the affinity for PCNA and Pol-delta4 processivity. May be phosphorylated by CDK1-cyclin-A complex, as well as CDK2-cyclin-A and CDK2-cyclin-E complexes. PCNA interferes with CDK-cyclin phosphorylation. {ECO:0000250|UniProtKB:Q15054}.
uniprot
proteins
pubmed
10219083
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
27524497
uniprot
proteins
sequence_length
462
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27524497}. Nucleus {ECO:0000269|PubMed:27524497}. Note=Partially colocalizes with PCNA and POLD1 at S phase replication sites. Recruited to DNA damage sites within 2 hours following UV irradiation. {ECO:0000250|UniProtKB:Q15054}.
uniprot
proteins