NCBP1
entrez
4686
canonical
uniprot
Q09161
canonical
ensembl
ENSG00000136937
resolver
ensp
ENSP00000364289
resolver
enst
ENST00000375147
resolver
genesymbol
NCBP1
resolver
genesymbol_syn
CBP80
resolver
genesymbol_syn
NCBP
resolver
hgnc
HGNC:7658
resolver
uniprot
B2R718
resolver
uniprot
Q59G76
resolver
uniprot
Q5T1V0
resolver
uniprot
Q5T7X2
resolver
uniprot_entry
NCBP1_HUMAN
resolver
uniprot_sec
B2R718
resolver
uniprot_sec
Q59G76
resolver
uniprot_sec
Q5T1V0
resolver
uniprot_sec
Q5T7X2
resolver
amino_acid_sequence
MSRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLSQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSFSHSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGVLEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSVLTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA
uniprot
proteins
function
FUNCTION: Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858). {ECO:0000269|PubMed:11551508, ECO:0000269|PubMed:12093754, ECO:0000269|PubMed:15059963, ECO:0000269|PubMed:15361857, ECO:0000269|PubMed:16186820, ECO:0000269|PubMed:16317009, ECO:0000269|PubMed:17190602, ECO:0000269|PubMed:17873884, ECO:0000269|PubMed:18369367, ECO:0000269|PubMed:19632182, ECO:0000269|PubMed:19648179, ECO:0000269|PubMed:26382858, ECO:0000269|PubMed:7651522, ECO:0000269|PubMed:8069914}.
uniprot
proteins
mass_dalton
91839
uniprot
proteins
mutagenesis
MUTAGEN 7; /note="S->A: Reduced phosphorylation by RPS6KB1. Abolishes phosphorylation by RPS6KB1; when associated with 21-A-A-22."; /evidence="ECO:0000269|PubMed:10973943"; MUTAGEN 17..18; /note="KR->AA: Abolishes nuclear localization and phosphorylation by RPS6KB1."; /evidence="ECO:0000269|PubMed:10973943"; MUTAGEN 21..22; /note="TS->A: Reduced phosphorylation by RPS6KB1. Abolishes phosphorylation by RPS6KB1; when associated with A-7."; /evidence="ECO:0000269|PubMed:10973943
uniprot
proteins
post_translational_modification
PTM: Dephosphorylated at Thr-21 by the PNUTS-PP1 complex during RNA polymerase II transcription pause-release. {ECO:0000269|PubMed:39603239}.
uniprot
proteins
protein_family
NCBP1 family
uniprot
proteins
pubmed
10973943
uniprot
proteins
pubmed
11340157
uniprot
proteins
pubmed
11545740
uniprot
proteins
pubmed
11551508
uniprot
proteins
pubmed
12093754
uniprot
proteins
pubmed
12374755
uniprot
proteins
pubmed
12434151
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15059963
uniprot
proteins
pubmed
15164053
uniprot
proteins
pubmed
15361857
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16186820
uniprot
proteins
pubmed
16317009
uniprot
proteins
pubmed
17081983
uniprot
proteins
pubmed
17190602
uniprot
proteins
pubmed
17289661
uniprot
proteins
pubmed
17873884
uniprot
proteins
pubmed
18369367
uniprot
proteins
pubmed
18423201
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
18691976
uniprot
proteins
pubmed
19608861
uniprot
proteins
pubmed
19632182
uniprot
proteins
pubmed
19648179
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
25220460
uniprot
proteins
pubmed
26382858
uniprot
proteins
pubmed
27117702
uniprot
proteins
pubmed
27871484
uniprot
proteins
pubmed
28112733
uniprot
proteins
pubmed
30842217
uniprot
proteins
pubmed
34564892
uniprot
proteins
pubmed
39603239
uniprot
proteins
pubmed
7651522
uniprot
proteins
pubmed
7937105
uniprot
proteins
pubmed
8069914
uniprot
proteins
pubmed
9111328
uniprot
proteins
sequence_length
790
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19648179}. Cytoplasm {ECO:0000269|PubMed:17289661}. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. {ECO:0000269|PubMed:17289661}.
uniprot
proteins