EME1
entrez
146956
canonical
uniprot
Q96AY2
canonical
ensembl
ENSG00000154920
resolver
ensp
ENSP00000339897
resolver
ensp
ENSP00000376952
resolver
ensp
ENSP00000597776
resolver
ensp
ENSP00000618387
resolver
ensp
ENSP00000759623
resolver
genesymbol
EME1
resolver
genesymbol_syn
MMS4
resolver
hgnc
HGNC:24965
resolver
uniprot
Q96N62
resolver
uniprot_entry
EME1_HUMAN
resolver
uniprot_sec
Q96N62
resolver
amino_acid_sequence
MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD
uniprot
proteins
function
FUNCTION: Non-catalytic subunit of the structure-specific, heterodimeric DNA endonuclease MUS81-EME1 which is involved in the maintenance of genome stability. In the complex, EME1 is required for DNA cleavage, participating in DNA recognition and bending (PubMed:12686547, PubMed:12721304, PubMed:14617801, PubMed:17289582, PubMed:24733841, PubMed:24813886, PubMed:35290797, PubMed:39015284). MUS81-EME1 cleaves 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs (PubMed:24733841, PubMed:35290797). Active during prometaphase, MUS81-EME1 resolves mitotic recombination intermediates, including Holliday junctions, which form during homologous recombination (PubMed:14617801, PubMed:24813886). {ECO:0000269|PubMed:12686547, ECO:0000269|PubMed:12721304, ECO:0000269|PubMed:14617801, ECO:0000269|PubMed:17289582, ECO:0000269|PubMed:24733841, ECO:0000269|PubMed:24813886, ECO:0000269|PubMed:35290797, ECO:0000269|PubMed:39015284}.
uniprot
proteins
mass_dalton
63252
uniprot
proteins
mutagenesis
MUTAGEN 491; /note="R->E: Loss of endonuclease activity; when associated with W-493."; /evidence="ECO:0000269|PubMed:24733841"; MUTAGEN 493; /note="S->W: Loss of endonuclease activity; when associated with E-491."; /evidence="ECO:0000269|PubMed:24733841"; MUTAGEN 534; /note="R->E: Decreased endonuclease activity; when associated with Y-541."; /evidence="ECO:0000269|PubMed:24733841"; MUTAGEN 541; /note="T->Y: Decreased endonuclease activity; when associated with E-534."; /evidence="ECO:0000269|PubMed:24733841
uniprot
proteins
protein_family
EME1/MMS4 family
uniprot
proteins
pubmed
12686547
uniprot
proteins
pubmed
12721304
uniprot
proteins
pubmed
14617801
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
17081983
uniprot
proteins
pubmed
17289582
uniprot
proteins
pubmed
18220336
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24733841
uniprot
proteins
pubmed
24813886
uniprot
proteins
pubmed
28112733
uniprot
proteins
pubmed
35290797
uniprot
proteins
pubmed
39015284
uniprot
proteins
sequence_length
570
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:14617801}. Note=Recruited to regions of DNA damage in S-phase cells.
uniprot
proteins