Cyp51a1
entrez
25427
canonical
uniprot
Q64654
canonical
ensembl
ENSRNOG00000007234
resolver
ensembl
ENSRNOG00055001760
resolver
ensembl
ENSRNOG00060016934
resolver
ensembl
ENSRNOG00065018261
resolver
ensp
ENSRNOP00000009985
resolver
ensp
ENSRNOP00055002121
resolver
ensp
ENSRNOP00060023314
resolver
ensp
ENSRNOP00065024339
resolver
enst
ENSRNOT00000009985
resolver
enst
ENSRNOT00055002837
resolver
enst
ENSRNOT00060028958
resolver
enst
ENSRNOT00065030601
resolver
genesymbol
Cyp51a1
resolver
genesymbol_syn
Cyp51
resolver
uniprot
Q64549
resolver
uniprot_entry
CP51A_RAT
resolver
uniprot_sec
Q64549
resolver
activity_regulation
ACTIVITY REGULATION: Inhibited by azalanstat (PubMed:7665087). Inhibited by azole antifungal agents ketoconazole, itraconazole and fluconazole (PubMed:10544287). {ECO:0000269|PubMed:10544287, ECO:0000269|PubMed:7665087}.
uniprot
proteins
amino_acid_sequence
MVLLGLLQSGGSVLGQAMEQVTGGNLLSTLLIACAFTLSLVYLFRLAVGHMVQLPAGAKSPPYIYSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEEVYGRLTTPVFGKGVAYDVPNAVFLEQKKILKSGLNIAHFKQYVSIIEKEAKEYFKSWGESGERNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKNIFYKAIQKRRLSKEPAEDILQTLLDSTYKDGRPLTDDEIAGMLIGLLLAGQHTSSTTSAWMGFFLARDKPLQDKCYLEQKTVCGEDLPPLTYEQLKDLNLLDRCIKETLRLRPPIMTMMRMAKTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLINGYFPSVNYTTMIHTPENPVIRYKRRSK
uniprot
proteins
ec_number
1.14.14.154
uniprot
proteins
function
FUNCTION: Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis (PubMed:10544287, PubMed:7665087). Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25-dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4-dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis (PubMed:10544287, PubMed:11328599, PubMed:1872829, PubMed:3782148, PubMed:7665087). Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25-dihydrolanosterol), but at a lower rate than DHL (PubMed:1872829). {ECO:0000269|PubMed:10544287, ECO:0000269|PubMed:11328599, ECO:0000269|PubMed:1872829, ECO:0000269|PubMed:3782148, ECO:0000269|PubMed:7665087}.
uniprot
proteins
mass_dalton
56707
uniprot
proteins
mutagenesis
MUTAGEN 130; /note="V->A: Decreases lanosterol 14-alpha demethylase activity."; /evidence="ECO:0000269|PubMed:11328599"; MUTAGEN 136; /note="T->A,V: Decreases lanosterol 14-alpha demethylase activity."; /evidence="ECO:0000269|PubMed:11328599"; MUTAGEN 139; /note="F->A: Impairs lanosterol 14-alpha demethylase activity."; /evidence="ECO:0000269|PubMed:11328599"; MUTAGEN 144; /note="A->I,V: Decreases lanosterol 14-alpha demethylase activity. Increases the susceptibility to ketoconazole."; /evidence="ECO:0000269|PubMed:11328599"; MUTAGEN 227; /note="Y->A: Significantly decreases lanosterol 14-alpha demethylase activity. Increases the susceptibility to ketoconazole."; /evidence="ECO:0000269|PubMed:11328599"; MUTAGEN 231; /note="D->A,E: Significantly decreases lanosterol 14-alpha demethylase activity."; /evidence="ECO:0000269|PubMed:11328599"; MUTAGEN 314; /note="H->F,A,K,D: Significantly decreases lanosterol 14-alpha demethylase activity. Increases the susceptibility to ketoconazole."; /evidence="ECO:0000269|PubMed:11328599"; MUTAGEN 315; /note="T->A,V,K,N: Impairs lanosterol 14-alpha demethylase activity. Increases the susceptibility to ketoconazole."; /evidence="ECO:0000269|PubMed:11328599"; MUTAGEN 316; /note="S->T,V,L: Significantly decreases lanosterol 14-alpha demethylase activity."; /evidence="ECO:0000269|PubMed:11328599
uniprot
proteins
pathway_participation
PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6. {ECO:0000305|PubMed:7665087}.
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated by MARCHF6, leading to proteasomal degradation. {ECO:0000250|UniProtKB:Q16850}.
uniprot
proteins
protein_family
Cytochrome P450 family
uniprot
proteins
pubmed
10544287
uniprot
proteins
pubmed
11328599
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
1872829
uniprot
proteins
pubmed
3782148
uniprot
proteins
pubmed
7665087
uniprot
proteins
pubmed
8024575
uniprot
proteins
pubmed
8797093
uniprot
proteins
pubmed
9498553
uniprot
proteins
sequence_length
503
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305|PubMed:8797093}; Single-pass membrane protein {ECO:0000255}. Microsome membrane {ECO:0000269|PubMed:8797093}; Single-pass membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 24..44; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins