C16orf74
entrez
404550
canonical
uniprot
Q96GX8
canonical
ensembl
ENSG00000154102
resolver
ensp
ENSP00000284245
resolver
genesymbol
C16orf74
resolver
hgnc
HGNC:23362
resolver
uniprot
R4GN89
resolver
uniprot_entry
CP074_HUMAN
resolver
uniprot_sec
R4GN89
resolver
amino_acid_sequence
MGLKMSCLKGFQMCVSSSSSSHDEAPVLNDKHLDVPDIIITPPTPTGMMLPRDLGSTVWLDETGSCPDDGEIDPEA
uniprot
proteins
function
FUNCTION: Associates with and recruits the catalytic subunit of the calcineurin complex (PPP3CA/Calcineurin A) to the plasma membrane, where it functions as a dynamic scaffold and regulator of PPP3CA activity thereby modulating calcineurin signaling (PubMed:41224739). In brown adipocytes, regulates thermogenesis by binding to PPP3CA, promoting the dephosphorylation of the PKA regulatory subunit, PRKAR2B. This modulation of PRKAR2B phosphorylation enhances PKA activity, thereby stimulating heat thermogenesis (By similarity). {ECO:0000250|UniProtKB:Q8K1L6, ECO:0000269|PubMed:41224739}.
uniprot
proteins
mass_dalton
8118
uniprot
proteins
mutagenesis
MUTAGEN 2; /note="G->A: Abolishes localization to membrane. Leads to cytosolic relocalization."; /evidence="ECO:0000269|PubMed:41224739"; MUTAGEN 7; /note="C->A: Abolishes localization to membrane. Leads to cytosolic relocalization; when associated with A-14."; /evidence="ECO:0000269|PubMed:41224739"; MUTAGEN 14; /note="C->A: Abolishes localization to membrane. Leads to cytosolic relocalization.; when associated with A-7."; /evidence="ECO:0000269|PubMed:41224739"; MUTAGEN 33; /note="L->A: Decreased interaction with PPP3CA; when associated with A-35. Reduced dephosphorylation of Tyr-44 by PPP3CA; when associated with A-35."; /evidence="ECO:0000269|PubMed:41224739"; MUTAGEN 35; /note="V->A: Decreased interaction with PPP3CA; when associated with A-35. Reduced dephosphorylation of Tyr-44 by PPP3CA; when associated with A-35."; /evidence="ECO:0000269|PubMed:41224739"; MUTAGEN 36..41; /note="Missing: Abolishes interaction with PPP3CA."; /evidence="ECO:0000269|PubMed:28881575"; MUTAGEN 38; /note="I->A: Abolished interaction with PPP3CA; when associated with A-40 and A-41. Reduced the localization of PPP3CA to membrane leading to its cytosolic relocalization; when associated with A-40 and A-41."; /evidence="ECO:0000269|PubMed:41224739"; MUTAGEN 40; /note="I->A: Abolished interaction with PPP3CA; when associated with A-38 and A-41. Reduced the localization of PPP3CA to membrane leading to its cytosolic relocalization; when associated with A-40 and A-41."; /evidence="ECO:0000269|PubMed:41224739"; MUTAGEN 41; /note="T->A: No effect on phosphorylation. Abolished interaction with PPP3CA; when associated with A-38 and A-40."; /evidence="ECO:0000269|PubMed:28881575, ECO:0000269|PubMed:41224739"; MUTAGEN 44; /note="T->A: Abolished phosphorylation and interaction with PPP3CA."; /evidence="ECO:0000269|PubMed:28881575
uniprot
proteins
post_translational_modification
PTM: N-myristoylation, in combination with reversible S-acylation promote the association of CLMB with membranes. {ECO:0000269|PubMed:41224739}.; PTM: Phosphorylated. Phosphorylation and dephosphorylation of CLMB regulate its interaction with PPP3CA, controlling its recruitment to the plasma membrane and modulate calcineurin signaling. Phosphorylation at Thr-44 increases CLMB binding to PPP3CA. Dephosphorylated by PPP3CA requires two CLMB molecules per PPP3CA allowing one CLMB to bind PPP3CA via its PxIxIT and the other CLMB to undergo LxVP-dependent dephosphorylation. {ECO:0000269|PubMed:41224739}.
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15616553
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
28881575
uniprot
proteins
pubmed
40355556
uniprot
proteins
pubmed
41224739
uniprot
proteins
sequence_length
76
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:40355556}. Membrane {ECO:0000269|PubMed:41224739}. Note=Associates with membranes via lipidation. {ECO:0000269|PubMed:41224739}.
uniprot
proteins