Srsf1
entrez
110809
canonical
uniprot
Q6PDM2
canonical
ensembl
ENSMUSG00000018379
resolver
ensp
ENSMUSP00000120595
resolver
ensp
ENSMUSP00000133517
resolver
genesymbol
Srsf1
resolver
genesymbol_syn
Sfrs1
resolver
uniprot
B2KGJ5
resolver
uniprot
Q3UCH2
resolver
uniprot
Q5SXC5
resolver
uniprot
Q8BJV3
resolver
uniprot
Q8C1H9
resolver
uniprot_entry
SRSF1_MOUSE
resolver
uniprot_sec
B2KGJ5
resolver
uniprot_sec
Q3UCH2
resolver
uniprot_sec
Q5SXC5
resolver
uniprot_sec
Q8BJV3
resolver
uniprot_sec
Q8C1H9
resolver
amino_acid_sequence
MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRT
uniprot
proteins
function
FUNCTION: Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing (PubMed:28785060). Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing (By similarity). Specifically regulates alternative splicing of cardiac isoforms of CAMK2D, LDB3/CYPHER and TNNT2/CTNT during heart remodeling at the juvenile to adult transition. The inappropriate accumulation of a neonatal and neuronal isoform of CAMKD2 in the adult heart results in aberrant calcium handling and defective excitation-contraction coupling in cardiomyocytes. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway (PubMed:15652482). {ECO:0000250|UniProtKB:Q07955, ECO:0000269|PubMed:15652482, ECO:0000269|PubMed:28785060}.
uniprot
proteins
mass_dalton
27745
uniprot
proteins
post_translational_modification
PTM: Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Phosphorylated by SRPK1 at multiple serines in its RS domain via a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds to a docking groove in the large lobe of the kinase domain of SRPK1 and this induces certain structural changes in SRPK1 and/or RRM 2 domain of SRSF1, allowing RRM 2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM 2, which then docks at the docking groove of SRPK1. This also signals RRM 2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed (By similarity). {ECO:0000250|UniProtKB:Q07955}.; PTM: Asymmetrically dimethylated at arginines, probably by PRMT1, methylation promotes localization to nuclear speckles. {ECO:0000250|UniProtKB:Q07955}.
uniprot
proteins
protein_family
Splicing factor SR family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15652482
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19131326
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
24129315
uniprot
proteins
pubmed
28785060
uniprot
proteins
pubmed
9307018
uniprot
proteins
sequence_length
248
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Chromosome {ECO:0000250|UniProtKB:Q07955}. Cytoplasm {ECO:0000250|UniProtKB:Q07955}. Nucleus speckle {ECO:0000269|PubMed:28785060}. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm. Nuclear import is mediated via interaction with TNPO3. {ECO:0000250|UniProtKB:Q07955}.
uniprot
proteins