P24462
uniprot
P24462
canonical
amino_acid_sequence
MDLIPNLAVETWLLLAVSLILLYLYGTRTHGLFKKLGIPGPTPLPFLGNALSFRKGYWTFDMECYKKYRKVWGIYDCQQPMLAITDPDMIKTVLVKECYSVFTNRRPFGPVGFMKNAISIAEDEEWKRIRSLLSPTFTSGKLKEMVPIIAQYGDVLVRNLRREAETGKPVTLKHVFGAYSMDVITSTSFGVSIDSLNNPQDPFVENTKKLLRFNPLDPFVLSIKVFPFLTPILEALNITVFPRKVISFLTKSVKQIKEGRLKETQKHRVDFLQLMIDSQNSKDSETHKALSDLELMAQSIIFIFAGYETTSSVLSFIIYELATHPDVQQKVQKEIDTVLPNKAPPTYDTVLQLEYLDMVVNETLRLFPVAMRLERVCKKDVEINGMFIPKGVVVMIPSYVLHHDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALVNMKLALVRVLQNFSFKPCKETQIPLKLRFGGLLLTEKPIVLKAESRDETVSGA
uniprot
proteins
ec_number
1.14.14.1
uniprot
proteins
function
FUNCTION: A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins during embryogenesis (PubMed:11093772, PubMed:12865317, PubMed:14559847, PubMed:17178770, PubMed:9555064). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:11093772, PubMed:12865317, PubMed:14559847, PubMed:17178770, PubMed:9555064). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA), a precursor in the biosynthesis of androgen and estrogen steroid hormones (PubMed:17178770, PubMed:9555064). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1), particularly D-ring hydroxylated estrone at the C16-alpha position (PubMed:12865317, PubMed:14559847). Mainly hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in atRA clearance during fetal development (PubMed:11093772). Also involved in the oxidative metabolism of xenobiotics including anticonvulsants (PubMed:9555064). {ECO:0000269|PubMed:11093772, ECO:0000269|PubMed:12865317, ECO:0000269|PubMed:14559847, ECO:0000269|PubMed:17178770, ECO:0000269|PubMed:9555064}.
uniprot
proteins
mass_dalton
57470
uniprot
proteins
mutagenesis
MUTAGEN 116; /note="N->S: Has no effect on catalytic activity."; /evidence="ECO:0000269|PubMed:17178770"; MUTAGEN 174; /note="H->D: Increases catalytic activity."; /evidence="ECO:0000269|PubMed:17178770"; MUTAGEN 214; /note="N->D: Increases catalytic activity."; /evidence="ECO:0000269|PubMed:17178770"; MUTAGEN 224; /note="K->T: Reduces affinity for substrate and catalytic efficiency."; /evidence="ECO:0000269|PubMed:17178770"; MUTAGEN 244; /note="K->E: Reduces affinity for substrate and catalytic efficiency."; /evidence="ECO:0000269|PubMed:17178770"; MUTAGEN 262; /note="K->E: Has no effect on catalytic activity."; /evidence="ECO:0000269|PubMed:17178770
uniprot
proteins
pathway_participation
PATHWAY: Steroid hormone biosynthesis. {ECO:0000269|PubMed:12865317, ECO:0000269|PubMed:14559847, ECO:0000269|PubMed:17178770}.; PATHWAY: Cofactor metabolism; retinol metabolism. {ECO:0000269|PubMed:11093772}.
uniprot
proteins
protein_family
Cytochrome P450 family
uniprot
proteins
pubmed
11093772
uniprot
proteins
pubmed
11137287
uniprot
proteins
pubmed
11266076
uniprot
proteins
pubmed
12690205
uniprot
proteins
pubmed
12865317
uniprot
proteins
pubmed
14559847
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
17178770
uniprot
proteins
pubmed
2492179
uniprot
proteins
pubmed
2722762
uniprot
proteins
pubmed
2786707
uniprot
proteins
pubmed
9555064
uniprot
proteins
sequence_length
503
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane {ECO:0000305|PubMed:2492179}; Peripheral membrane protein.
uniprot
proteins