Slc22a1
entrez
20517
canonical
uniprot
O08966
canonical
ensembl
ENSMUSG00000023829
resolver
ensp
ENSMUSP00000024596
resolver
enst
ENSMUST00000024596
resolver
genesymbol
Slc22a1
resolver
genesymbol_syn
Lx1
resolver
genesymbol_syn
Oct1
resolver
uniprot
Q9R1Q4
resolver
uniprot_entry
S22A1_MOUSE
resolver
uniprot_sec
Q9R1Q4
resolver
activity_regulation
ACTIVITY REGULATION: Phosphorylation of the transporter leads to changes in its substrate affinity, resulting in a regulation of the transport activity. In contrast with rat ortholog, ASP uptake is inhibited by protein kinase A (PKA) and C (PKC) activation. ASP uptake is also endogenously activated by calmodulin, the calmodulin-dependent kinase II and LCK tyrosine kinase (By similarity). Inhibited by cGMP, most likely through a cGMP-binding protein that interacts with OCT1 (By similarity). {ECO:0000250|UniProtKB:O15245, ECO:0000250|UniProtKB:Q63089}.
uniprot
proteins
amino_acid_sequence
MPTVDDVLEHVGEFGWFQKQAFLLLCLISASLAPIYVGIVFLGFTPDHHCRSPGVAELSQRCGWSPAEELNYTVPGLGSAGEASFLSQCMKYEVDWNQSTLDCVDPLSSLAANRSHLPLSPCEHGWVYDTPGSSIVTEFNLVCGDAWKVDLFQSCVNLGFFLGSLVVGYIADRFGRKLCLLVTTLVTSLSGVLTAVAPDYTSMLLFRLLQGMVSKGSWVSGYTLITEFVGSGYRRTTAILYQVAFTVGLVGLAGVAYAIPDWRWLQLAVSLPTFLFLLYYWFVPESPRWLLSQKRTTQAVRIMEQIAQKNRKVPPADLKMMCLEEDASERRSPSFADLFRTPSLRKHTLILMYLWFSCAVLYQGLIMHVGATGANLYLDFFYSSLVEFPAAFIILVTIDRIGRIYPIAASNLVAGAACLLMIFIPHELHWLNVTLACLGRMGATIVLQMVCLVNAELYPTFIRNLGMMVCSALCDLGGIFTPFMVFRLMEVWQALPLILFGVLGLSAGAVTLLLPETKGVALPETIEEAENLGRRKSKAKENTIYLQVQTGKSPHT
uniprot
proteins
function
FUNCTION: Electrogenic voltage-dependent uniporter that mediates the transport of a variety of organic cations such as endogenous bioactive amines, cationic drugs and xenobiotics (PubMed:10216142, PubMed:11463829, PubMed:12176030, PubMed:23458604, PubMed:24961373). Functions as a pH- and Na(+)-independent, bidirectional transporter (By similarity). Cation cellular uptake or release is driven by the electrochemical potential (i.e. membrane potential and concentration gradient) and substrate selectivity (By similarity). Hydrophobicity is a major requirement for recognition in polyvalent substrates and inhibitors (PubMed:23458604). Primarily expressed in the basolateral membrane of hepatocytes and proximal tubules and involved in the uptake and disposition of cationic compounds from the blood by hepatic and renal clearance (By similarity). Most likely functions as an uptake carrier in enterocytes contributing to the intestinal elimination of organic cations from the systemic circulation (PubMed:11463829, PubMed:24961373). Transports endogenous monoamines such as N-1-methylnicotinamide (NMN), guanidine, neurotransmitters dopamine, serotonin, noradrenaline, adrenaline and histamine, and quaternary ammonium compound such as choline (PubMed:24961373, PubMed:35469921). Also transports natural polyamines such as spermidine, agmatine and putrescine at low affinity, but relatively high turnover (PubMed:23458604). Involved in the hepatic and intestinal uptake of the vitamin B1/thiamine, hence regulating hepatic lipid and energy metabolism (PubMed:24961373). Contributes to the influx and efflux of fatty acid carriers carnitines and acylcarnitines across the basolateral membrane of hepatocytes, from the liver to the systemic circulation and inversely and may be involved in regulating the systemic availability of hepatic acylcarnitines (PubMed:28942964, PubMed:34040533). Also capable of transporting non-amine endogenous compounds such as prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha) (By similarity). May contribute to the transport of cationic compounds in testes across the blood-testis-barrier (By similarity). Also mediates the uptake of xenobiotics tributylmethylammonium (TBuMA), quinidine, N-methyl-quinine (NMQ), N-methyl-quinidine (NMQD) N-(4,4-azo-n-pentyl)-quinuclidine (APQ), azidoprocainamide methoiodide (AMP), N-(4,4-azo-n-pentyl)-21-deoxyajmalinium (APDA) and 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP) (PubMed:11463829). {ECO:0000250|UniProtKB:O15245, ECO:0000250|UniProtKB:Q63089, ECO:0000269|PubMed:10216142, ECO:0000269|PubMed:11463829, ECO:0000269|PubMed:12176030, ECO:0000269|PubMed:23458604, ECO:0000269|PubMed:24961373, ECO:0000269|PubMed:28942964, ECO:0000269|PubMed:34040533, ECO:0000269|PubMed:35469921}.
uniprot
proteins
mass_dalton
61521
uniprot
proteins
mutagenesis
MUTAGEN 32; /note="L->F: Increased trospium uptake. Increased trospium affinity. No change in fenoterol uptake."; /evidence="ECO:0000269|PubMed:35469921"; MUTAGEN 36; /note="Y->C: Decreased fenoterol uptake. Decreased fenoterol affinity. No change in trospium uptake. No change in terbutaline affinity."; /evidence="ECO:0000269|PubMed:35469921
uniprot
proteins
post_translational_modification
PTM: Phosphorylated. {ECO:0000250|UniProtKB:Q63089}.
uniprot
proteins
protein_family
Major facilitator (TC 2.A.1) superfamily
uniprot
proteins
protein_family
Organic cation transporter (TC 2.A.1.19) family
uniprot
proteins
pubmed
10216142
uniprot
proteins
pubmed
11463829
uniprot
proteins
pubmed
12176030
uniprot
proteins
pubmed
15458920
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16381671
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
23458604
uniprot
proteins
pubmed
23680637
uniprot
proteins
pubmed
24961373
uniprot
proteins
pubmed
28942964
uniprot
proteins
pubmed
34040533
uniprot
proteins
pubmed
35469921
uniprot
proteins
pubmed
8854860
uniprot
proteins
sequence_length
556
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Basolateral cell membrane {ECO:0000269|PubMed:28942964}; Multi-pass membrane protein {ECO:0000305}. Apical cell membrane {ECO:0000269|PubMed:23680637}; Multi-pass membrane protein {ECO:0000305}. Lateral cell membrane {ECO:0000250|UniProtKB:O15245}; Multi-pass membrane protein {ECO:0000305}. Basal cell membrane {ECO:0000250|UniProtKB:O15245}; Multi-pass membrane protein {ECO:0000305}. Note=Localized to the sinusoidal/basolateral membrane of hepatocytes (PubMed:28942964). Mainly localized to the basolateral membrane of renal proximal tubular cells (By similarity). However, also identified at the apical side of proximal tubular cells (By similarity). Mainly expressed at the lateral membrane of enterocytes (By similarity). Also observed at the apical side of enterocytes (PubMed:23680637). Localized to the basal membrane of Sertoli cells (By similarity). {ECO:0000250|UniProtKB:O15245, ECO:0000250|UniProtKB:Q63089, ECO:0000269|PubMed:23680637, ECO:0000269|PubMed:28942964}.
uniprot
proteins
transmembrane_region
TRANSMEM 22..42; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 151..171; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 178..198; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 212..231; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 239..259; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 264..284; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 349..369; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 378..398; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 404..424; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 430..452; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 466..486; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 494..514; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins