P23965
uniprot
P23965
canonical
amino_acid_sequence
MALAAARRVLLQAGSRLGRRGAVDGARRFSNKRVLVEKEGEAGIAVMKFKNPPVNSLSLEFLTEFVISLEKLENDKSIRGVILTSERPGIFSAGLDLMEMYGRNPAHYAEYWKAVQELWLRLYLSNLTLISAINGASPAGGCLMALTCDYRIMADNSKYTIGLNESLLGIVAPFWLKDNYVNTIGHRAAERALQLGTLFPPAEALKVGLVDEVVPEDQVHSKARSVMAKWFTIPDHSRQLTKSMMRKATADNLIKQREADIQNFTSFISRDSIQKSLHVYLEKLKQKKG
uniprot
proteins
ec_number
5.3.3.8
uniprot
proteins
function
FUNCTION: Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids (PubMed:11781327, PubMed:8218206). Together with the 2,4-dienoyl-CoA reductase (DECR) and the Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (ECH1), they allow the reentrance of the enoyl-CoA into the beta-oxidation cycle. Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2-trans (2E) form in a range of fatty enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (PubMed:11781327, PubMed:8218206). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length, and based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (Probable). {ECO:0000269|PubMed:11781327, ECO:0000269|PubMed:8218206, ECO:0000305|PubMed:11781327, ECO:0000305|PubMed:8218206}.
uniprot
proteins
mass_dalton
32254
uniprot
proteins
mutagenesis
MUTAGEN 165; /note="E->Q: Loss of activity."; /evidence="ECO:0000269|PubMed:8218206
uniprot
proteins
pathway_participation
PATHWAY: Lipid metabolism; fatty acid beta-oxidation. {ECO:0000269|PubMed:11781327, ECO:0000269|PubMed:8218206}.
uniprot
proteins
protein_family
Enoyl-CoA hydratase/isomerase family
uniprot
proteins
pubmed
11781327
uniprot
proteins
pubmed
15883186
uniprot
proteins
pubmed
1958319
uniprot
proteins
pubmed
2040594
uniprot
proteins
pubmed
8218206
uniprot
proteins
sequence_length
289
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000269|PubMed:11781327}.
uniprot
proteins