Mboat4
entrez
234155
canonical
uniprot
P0C7A3
canonical
ensembl
ENSMUSG00000071113
resolver
ensp
ENSMUSP00000092988
resolver
enst
ENSMUST00000095345
resolver
genesymbol
Mboat4
resolver
genesymbol_syn
Gm171
resolver
genesymbol_syn
Goat
resolver
uniprot
B1Q004
resolver
uniprot
B2RWL6
resolver
uniprot_entry
MBOA4_MOUSE
resolver
uniprot_sec
B1Q004
resolver
uniprot_sec
B2RWL6
resolver
activity_regulation
ACTIVITY REGULATION: Inhibited by 1-[2-cyano-3,12-dioxooleana-1,9(11)- dien-28-oyl]ethylamide (CDDO-EA) with an IC(50) of 60 uM (PubMed:28134508). Inhibited by Fe3+ and Cu2+ and the O-acyltransferase activity is completely blocked over 5 mM Fe3+ and 0.5 mM Cu2+ (PubMed:19501572). {ECO:0000269|PubMed:19501572, ECO:0000269|PubMed:28134508}.
uniprot
proteins
amino_acid_sequence
MDWLQLFFLHPLSFYQGAAFPFALLFNYLCILDTFSTRARYLFLLAGGGVLAFAAMGPYSLLIFIPALCAVALVSFLSPQEVHRLTFFFQMGWQTLCHLGLHYTEYYLGEPPPVRFYITLSSLMLLTQRVTSLSLDICEGKVEAPRRGIRSKSSFSEHLWDALPHFSYLLFFPALLGGSLCSFRRFQACVQRSSSLYPSISFRALTWRGLQILGLECLKVALRSAVSAGAGLDDCQRLECIYLMWSTAWLFKLTYYSHWILDDSLLHAAGFGAEAGQGPGEEGYVPDVDIWTLETTHRISLFARQWNRSTALWLRRLVFRKSRRWPLLQTFAFSAWWHGLHPGQVFGFLCWSVMVKADYLIHTFANVCIRSWPLRLLYRALTWAHTQLIIAYIMLAVEGRSLSSLCQLCCSYNSLFPVMYGLLLFLLAERKDKRN
uniprot
proteins
ec_number
2.3.1.-
uniprot
proteins
function
FUNCTION: Catalyzes ghrelin acylation at 'Ser-3' using preferentially octanoyl-CoA, hexanoyl-CoA and decanoyl-CoA as acyl-CoA donors leading to ghrelin activity (PubMed:18267071, PubMed:18443287, PubMed:18669668, PubMed:19501572, PubMed:24045953, PubMed:28134508). In vitro also uses acyl-CoA donors of different lengths from short-chain (C2) to long-chain fatty acids (C16) knowing that acyl-CoA donors from butanoyl-CoA (C4) to dodecanoyl-CoA (C12) are more efficient compared to longer acyl-CoA donors, such as myristoyl-CoA (C14) and palmitoyl-CoA (C16) that are not efficient (PubMed:19501572). {ECO:0000269|PubMed:18267071, ECO:0000269|PubMed:18443287, ECO:0000269|PubMed:18669668, ECO:0000269|PubMed:19501572, ECO:0000269|PubMed:24045953, ECO:0000269|PubMed:28134508}.; FUNCTION: [44,4 kDa fragment]: Inactive octanoyltransferase activity. {ECO:0000269|PubMed:24045953}.
uniprot
proteins
mass_dalton
49879
uniprot
proteins
mutagenesis
MUTAGEN 27; /note="N->H: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 56; /note="M->A: Loss of 44,4 kDa fragment."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 56; /note="M->I: Loss of 44,4 kDa form."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 149; /note="I->N: Does not glycosylated."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 181; /note="C->A: No effect on ability to acylate ghrelin."; /evidence="ECO:0000269|PubMed:18267071"; MUTAGEN 191; /note="Q->N: Does not glycosylated."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 219; /note="K->R: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 252; /note="K->R: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 290; /note="I->N: Does not glycosylated."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 293; /note="L->N: Does not glycosylated."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 298; /note="R->N: Does not glycosylated."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 307; /note="N->A: Abolished ability to acylate ghrelin."; /evidence="ECO:0000269|PubMed:18267071"; MUTAGEN 307; /note="N->H: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 309; /note="S->A: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 321; /note="K->R: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 338; /note="H->A: Abolished ability to acylate ghrelin. Does not affect proteic cleavage. loss of serine O-acyltransferase activity."; /evidence="ECO:0000269|PubMed:18267071, ECO:0000269|PubMed:18669668, ECO:0000269|PubMed:24045953"; MUTAGEN 338; /note="H->N: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 356; /note="K->R: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 366; /note="N->H: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 368; /note="C->A: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 431; /note="K->R: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953"; MUTAGEN 433; /note="K->R: Does not affect protein cleavage."; /evidence="ECO:0000269|PubMed:24045953
uniprot
proteins
post_translational_modification
PTM: Not glycosylated. {ECO:0000269|PubMed:24045953}.
uniprot
proteins
protein_family
Membrane-bound acyltransferase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
18267071
uniprot
proteins
pubmed
18443287
uniprot
proteins
pubmed
18669668
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
19501572
uniprot
proteins
pubmed
24045953
uniprot
proteins
pubmed
28134508
uniprot
proteins
sequence_length
435
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:18267071, ECO:0000269|PubMed:24045953}; Multi-pass membrane protein {ECO:0000269|PubMed:18267071}.
uniprot
proteins
transmembrane_region
TRANSMEM 6..26; /note="Helical"; /evidence="ECO:0000255, ECO:0000305|PubMed:24045953"; TRANSMEM 41..56; /note="Helical"; /evidence="ECO:0000255, ECO:0000305|PubMed:24045953"; TRANSMEM 60..76; /note="Helical"; /evidence="ECO:0000305|PubMed:24045953"; TRANSMEM 83..101; /note="Helical"; /evidence="ECO:0000305|PubMed:24045953"; TRANSMEM 121..136; /note="Helical"; /evidence="ECO:0000305|PubMed:24045953"; TRANSMEM 207..227; /note="Helical"; /evidence="ECO:0000305|PubMed:24045953"; TRANSMEM 241..261; /note="Helical"; /evidence="ECO:0000255, ECO:0000305|PubMed:24045953"; TRANSMEM 325..338; /note="Helical"; TRANSMEM 341..357; /note="Helical"; TRANSMEM 377..397; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 408..428; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins