P53349
uniprot
P53349
canonical
activity_regulation
ACTIVITY REGULATION: Activated by autophosphorylation on Thr-1381 and Thr-1393 following oligomerization. {ECO:0000269|PubMed:14500727, ECO:0000269|PubMed:9078260}.
uniprot
proteins
amino_acid_sequence
MAAAAGDRASSSGFPGAAAASPEAGGGGGGGGALQGSGAPAAGAAGLLREPGSAGRERADWRRRQLRKVRSVELDQLPEQPLFLAAASPPCPSTSPSPEPADAAAGASRFQPAAGPPPPGAASRCGSHSAELAAARDSGARSPAGAEPPSAAAPSGREMENKETLKGLHKMEDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKGDGSEVNNLAAEPQGEGQAGSAAPAPKGRRSPSPGSSPSGRSVKPESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGAFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVESPASLRAVQQPSSPQQPVAGSQRRNQESSFNLTHFGTQQIPSAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGGSGGSLSAGAASGSSQPSISGDVVEACCSVLSIVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTVLELCKGQAGELAVGREILKAGSIGVGGVDYVLSCILGNQAESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQAVAPTSCLENSSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSDETVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVAVRCLELQPQDRPPSRELLKHPVFRTTW
uniprot
proteins
ec_number
2.7.11.25
uniprot
proteins
function
FUNCTION: Component of a protein kinase signal transduction cascade (PubMed:14500727). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed:14500727). May phosphorylate the MAPK8/JNK1 kinase (PubMed:17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed:14500727). {ECO:0000269|PubMed:14500727, ECO:0000269|PubMed:17761173}.
uniprot
proteins
mass_dalton
161289
uniprot
proteins
mutagenesis
MUTAGEN 1381; /note="T->A: Fails to activate MAP2K1, MAP2K4, MAP2K7, CHUK and IKBKB."; /evidence="ECO:0000269|PubMed:14500727, ECO:0000269|PubMed:9078260"; MUTAGEN 1381; /note="T->E: Loss of kinase activity and autophosphorylation."; /evidence="ECO:0000269|PubMed:14500727, ECO:0000269|PubMed:9078260"; MUTAGEN 1381; /note="T->S: Reduced kinase activity and autophosphorylation."; /evidence="ECO:0000269|PubMed:14500727, ECO:0000269|PubMed:9078260"; MUTAGEN 1393; /note="T->A: Loss of kinase activity and autophosphorylation. Fails to activate MAP2K1, MAP2K4, MAP2K7, CHUK and IKBKB."; /evidence="ECO:0000269|PubMed:14500727, ECO:0000269|PubMed:9078260"; MUTAGEN 1393; /note="T->E: Loss of kinase activity and autophosphorylation."; /evidence="ECO:0000269|PubMed:14500727, ECO:0000269|PubMed:9078260"; MUTAGEN 1393; /note="T->S: Reduced kinase activity and autophosphorylation."; /evidence="ECO:0000269|PubMed:14500727, ECO:0000269|PubMed:9078260"; MUTAGEN 1394; /note="I->A: Loss of NF-kappa-B transcription factor activity and reduced ability to activate MAP2K1, MAP2K4, MAP2K7. No effect on AP-1 activity or activation of CHUK and IKBKB. Loss of binding to IKBKB."; /evidence="ECO:0000269|PubMed:14500727"; MUTAGEN 1396; /note="F->A: Loss of AP-1 and NF-kappa-B transcription factor activity. Reduced ability to activate MAP2K1, MAP2K4, MAP2K7, CHUK and IKBKB."; /evidence="ECO:0000269|PubMed:14500727"; MUTAGEN 1397; /note="M->A: Loss of AP-1 and NF-kappa-B transcription factor activity. Reduced ability to activate MAP2K1, MAP2K4, MAP2K7, CHUK and IKBKB."; /evidence="ECO:0000269|PubMed:14500727"; MUTAGEN 1401; /note="V->A: Loss of AP-1 and NF-kappa-B transcription factor activity. Reduced ability to activate MAP2K1, MAP2K4, MAP2K7, CHUK and IKBKB."; /evidence="ECO:0000269|PubMed:14500727"; MUTAGEN 1402; /note="L->A: Loss of AP-1 transcription factor activity and reduced ability to activate CHUK and IKBKB. No effect on NF-kappa-B activity or activation of MAP2K1, MAP2K4, MAP2K7. Loss of binding to MAP2K4."; /evidence="ECO:0000269|PubMed:14500727"; MUTAGEN 1403; /note="R->A: Loss of AP-1 transcription factor activity, no effect on NF-kappa-B activity."; /evidence="ECO:0000269|PubMed:14500727"; MUTAGEN 1404; /note="G->A: Loss of AP-1 and NF-kappa-B transcription factor activity."; /evidence="ECO:0000269|PubMed:14500727
uniprot
proteins
post_translational_modification
PTM: Autophosphorylated. {ECO:0000269|PubMed:9078260}.
uniprot
proteins
protein_family
MAP kinase kinase kinase subfamily
uniprot
proteins
protein_family
Protein kinase superfamily
uniprot
proteins
protein_family
STE Ser/Thr protein kinase family
uniprot
proteins
pubmed
10523642
uniprot
proteins
pubmed
14500727
uniprot
proteins
pubmed
15262978
uniprot
proteins
pubmed
17761173
uniprot
proteins
pubmed
19144319
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
8385802
uniprot
proteins
pubmed
9078260
uniprot
proteins
sequence_length
1493
uniprot
proteins