CHST2
entrez
9435
canonical
uniprot
Q9Y4C5
canonical
ensembl
ENSG00000175040
resolver
ensp
ENSP00000307911
resolver
genesymbol
CHST2
resolver
genesymbol
HEL-S-75
resolver
genesymbol_syn
GN6ST
resolver
hgnc
HGNC:1970
resolver
uniprot
D3DNG5
resolver
uniprot
Q2M370
resolver
uniprot
Q9GZN5
resolver
uniprot
Q9UED5
resolver
uniprot
Q9Y6F2
resolver
uniprot
V9HVX9
resolver
uniprot_entry
CHST2_HUMAN
resolver
uniprot_entry
V9HVX9_HUMAN
resolver
uniprot_sec
D3DNG5
resolver
uniprot_sec
Q2M370
resolver
uniprot_sec
Q9GZN5
resolver
uniprot_sec
Q9UED5
resolver
uniprot_sec
Q9Y6F2
resolver
amino_acid_sequence
MSRSPQRALPPGALPRLLQAAPAAAPRALLPQWPRRPGRRWPASPLGMKVFRRKALVLCAGYALLLVLTMLNLLDYKWHKEPLQQCNPDGPLGAAAGAAGGSWGRPGPPPAGPPRAHARLDLRTPYRPPAAAVGAAPAAAAGMAGVAAPPGNGTRGTGGVGDKRQLVYVFTTWRSGSSFFGELFNQNPEVFFLYEPVWHVWQKLYPGDAVSLQGAARDMLSALYRCDLSVFQLYSPAGSGGRNLTTLGIFGAATNKVVCSSPLCPAYRKEVVGLVDDRVCKKCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLRDPALDLKVIHLVRDPRAVASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGVGGPADYHALGAMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQFALNMTSGSGSSSKPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERVNSPEEVKDLSKTLLRKPRL
uniprot
proteins
ec_number
2.8.2.-
uniprot
proteins
function
FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N- and O-linked glycans and within O-linked mucin-type glycans (PubMed:11042394, PubMed:11726653, PubMed:35939855, PubMed:38034954, PubMed:9722682). Selectively transfers the sulfate group onto the terminal GlcNAc of alpha1,3-Man or alpha1,6-Man antenna of complex-type N-glycans depending on glycan composition. Only sulfates terminal GlcNAc of alpha1,3-Man antenna of G0 complex-type N-glycans. Can sulfate keratan-type N-acetyllactosamine (LacNAc) repeats generating epitopes for self versus non-self immune recognition by C-type lectins (PubMed:35939855, PubMed:38034954). Transfers the sulfate group primarily on core 2 GlcNAcbeta1-6(Galbeta1-3)GalNAcalpha-Ser/Thr and with lower efficiency on extended core 1 GlcNAcbeta1-3Galbeta1-3GalNAcalpha-Ser/Thr based O-linked glycans on peripheral node addressins (PNAds) expressed on the lumenal side of high endothelial venules (HEVs). Shares substrate specificity with CHST4 and both contribute to generate sialyl 6-sulfo Lewis X determinant (also known as MECA-79 epitope) for SELL recognition, a prerequisite for continuous lymphocyte homing into peripheral lymph nodes and antigen immune surveillance (By similarity) (PubMed:9722682). Has no activity toward alpha-linked GlcNAc moiety exposed at the non-reducing ends or to internally located GlcNAc residues (PubMed:11042394). {ECO:0000250|UniProtKB:Q80WV3, ECO:0000269|PubMed:11042394, ECO:0000269|PubMed:11726653, ECO:0000269|PubMed:35939855, ECO:0000269|PubMed:38034954, ECO:0000269|PubMed:9722682}.
uniprot
proteins
mass_dalton
57857
uniprot
proteins
mutagenesis
MUTAGEN 59; /note="C->S: Does not affect homodimerization. Abolishes homodimerization but not enzyme activity; when associated with S-39."; /evidence="ECO:0000269|PubMed:15220337"; MUTAGEN 86; /note="C->S: Induces migration in both homodimeric and monomeric forms. Abolishes homodimerization but not enzyme activity; when associated with S-12."; /evidence="ECO:0000269|PubMed:15220337"; MUTAGEN 174; /note="R->A: Induces a strong decrease in enzyme activity."; /evidence="ECO:0000269|PubMed:12501187"; MUTAGEN 296; /note="R->A: Induces a strong decrease in enzyme activity."; /evidence="ECO:0000269|PubMed:12501187"; MUTAGEN 304; /note="K->A: Loss of function."; /evidence="ECO:0000269|PubMed:12501187"; MUTAGEN 332; /note="R->A: Loss of function."; /evidence="ECO:0000269|PubMed:12501187"; MUTAGEN 341; /note="R->A: Induces a strong decrease in enzyme activity."; /evidence="ECO:0000269|PubMed:12501187"; MUTAGEN 457; /note="N->A: Reduced localization in the Golgi."; /evidence="ECO:0000269|PubMed:19571171"; MUTAGEN 475; /note="N->A: Unable to sulfate the sialyl Lewis X tetrasaccharide."; /evidence="ECO:0000269|PubMed:19571171"; MUTAGEN 518; /note="K->A: Has weak or no effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 519; /note="D->A: Has weak or no effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 520; /note="L->A: Has weak or no effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 521; /note="S->A: No effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 522; /note="K->A: No effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 523; /note="T->A: Has weak or no effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 524; /note="L->A,T: Induces a strong decrease in enzyme activity."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 525; /note="L->A: Induces a strong decrease in enzyme activity."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 525; /note="L->T: Has weak or no effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 526; /note="R->A: Has weak or no effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 527; /note="K->A: No effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 528; /note="P->A: Has weak or no effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 529; /note="R->A: No effect."; /evidence="ECO:0000269|PubMed:15632306"; MUTAGEN 530; /note="L->A,T: Induces a strong decrease in enzyme activity."; /evidence="ECO:0000269|PubMed:15632306
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; carbohydrate sulfation. {ECO:0000269|PubMed:11726653}.
uniprot
proteins
post_translational_modification
PTM: Glycosylation at Asn-475 is required for catalytic activity. {ECO:0000269|PubMed:19571171}.
uniprot
proteins
protein_family
Gal/GlcNAc/GalNAc subfamily
uniprot
proteins
protein_family
Sulfotransferase 1 family
uniprot
proteins
pubmed
10049591
uniprot
proteins
pubmed
11042394
uniprot
proteins
pubmed
11310842
uniprot
proteins
pubmed
11726653
uniprot
proteins
pubmed
12501187
uniprot
proteins
pubmed
12855678
uniprot
proteins
pubmed
15220337
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15632306
uniprot
proteins
pubmed
19571171
uniprot
proteins
pubmed
22260995
uniprot
proteins
pubmed
35939855
uniprot
proteins
pubmed
38034954
uniprot
proteins
pubmed
9722682
uniprot
proteins
sequence_length
530
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:12855678, ECO:0000269|PubMed:22260995}; Single-pass type II membrane protein {ECO:0000269|PubMed:12855678, ECO:0000269|PubMed:22260995}.
uniprot
proteins
transmembrane_region
TRANSMEM 55..75; /note="Helical; Signal-anchor for type II membrane protein"; /evidence="ECO:0000255
uniprot
proteins