Mpst
entrez
192172
canonical
uniprot
P97532
canonical
ensembl
ENSRNOG00000000185
resolver
ensembl
ENSRNOG00055032040
resolver
ensembl
ENSRNOG00060030521
resolver
ensembl
ENSRNOG00065033335
resolver
ensp
ENSRNOP00000000201
resolver
ensp
ENSRNOP00055045741
resolver
ensp
ENSRNOP00060044072
resolver
ensp
ENSRNOP00065047146
resolver
enst
ENSRNOT00000000201
resolver
enst
ENSRNOT00055055416
resolver
enst
ENSRNOT00060053015
resolver
enst
ENSRNOT00065057305
resolver
genesymbol
Mpst
resolver
uniprot_entry
THTM_RAT
resolver
activity_regulation
ACTIVITY REGULATION: By oxidative stress, and thioredoxin. Under oxidative stress conditions, the catalytic cysteine site is converted to a sulfenate which inhibits the MPST enzyme activity. Reduced thioredoxin cleaves an intersubunit disulfide bond to turn on the redox switch and reactivate the enzyme. Inhibited by different oxidants, hydrogen peroxide and tetrathionate. {ECO:0000269|PubMed:16107337, ECO:0000269|PubMed:17130129}.
uniprot
proteins
amino_acid_sequence
MAAPQLFRALVSAQWVAEALKSPRASQPLKLLDASWYLPKLGRDARREFEERHIPGAAFFDIDRCSDHTSPYDHMLPSATHFADYAGSLGVSAATHVVIYDGSDQGLYSAPRVWWMFRAFGHHSVSLLDGGFRYWLSQNLPISSGKSPSEPAEFCAQLDPSFIKTHEDILENLDARRFQVVDARAAGRFQGTQPEPRDGIEPGHIPGSVNIPFTEFLTSEGLEKSPEEIQRLFQEKKVDLSKPLVATCGSGVTACHVVLGAFLCGKPDVPVYDGSWVEWYMRAQPEHVISQGRGKTL
uniprot
proteins
ec_number
2.8.1.2
uniprot
proteins
function
FUNCTION: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions. {ECO:0000269|PubMed:16107337, ECO:0000269|PubMed:19605461, ECO:0000269|PubMed:8910318, ECO:0000269|PubMed:9749958}.
uniprot
proteins
mass_dalton
32940
uniprot
proteins
mutagenesis
MUTAGEN 65; /note="C->S: No effect on redox potential."; /evidence="ECO:0000269|PubMed:16107337"; MUTAGEN 155; /note="C->S: No effect on redox potential."; /evidence="ECO:0000269|PubMed:16107337"; MUTAGEN 188; /note="R->G: Large decrease in MST activity; some decrease in rhodanese activity."; /evidence="ECO:0000269|PubMed:8910318"; MUTAGEN 197; /note="R->G: Decreased MST activity; increased rhodanese activity."; /evidence="ECO:0000269|PubMed:8910318"; MUTAGEN 248; /note="C->S: Loss of both enzyme activities. Greatly reduced redox potential."; /evidence="ECO:0000269|PubMed:16107337, ECO:0000269|PubMed:8910318"; MUTAGEN 249; /note="G->R: Decreased MST activity; increased rhodanese activity."; MUTAGEN 250; /note="S->A: Slight decrease in MST activity."; /evidence="ECO:0000269|PubMed:8910318"; MUTAGEN 250; /note="S->K: Slight decrease in MST activity; increased rhodanese activity."; /evidence="ECO:0000269|PubMed:8910318"; MUTAGEN 255; /note="C->S: Little change in redox potential."; /evidence="ECO:0000269|PubMed:16107337"; MUTAGEN 264; /note="C->S: Greatly reduced redox potential."; /evidence="ECO:0000269|PubMed:16107337
uniprot
proteins
post_translational_modification
PTM: The N-terminus is blocked.
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16107337
uniprot
proteins
pubmed
17130129
uniprot
proteins
pubmed
19605461
uniprot
proteins
pubmed
7608189
uniprot
proteins
pubmed
8910318
uniprot
proteins
pubmed
9749958
uniprot
proteins
sequence_length
297
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9749958}. Mitochondrion {ECO:0000269|PubMed:9749958}. Synapse, synaptosome {ECO:0000250|UniProtKB:Q99J99}.
uniprot
proteins