MCRS1
entrez
10445
canonical
uniprot
Q96EZ8
canonical
ensembl
ENSG00000187778
resolver
ensp
ENSP00000345358
resolver
ensp
ENSP00000349640
resolver
ensp
ENSP00000444982
resolver
ensp
ENSP00000447514
resolver
ensp
ENSP00000448056
resolver
genesymbol
MCRS1
resolver
genesymbol_syn
INO80Q
resolver
genesymbol_syn
MSP58
resolver
hgnc
HGNC:6960
resolver
uniprot
O14742
resolver
uniprot
O75497
resolver
uniprot
Q6VN53
resolver
uniprot
Q7Z372
resolver
uniprot_entry
MCRS1_HUMAN
resolver
uniprot_sec
O14742
resolver
uniprot_sec
O75497
resolver
uniprot_sec
Q6VN53
resolver
uniprot_sec
Q7Z372
resolver
amino_acid_sequence
MDKDSQGLLDSSLMASGTASRSEDEESLAGQKRASSQALGTIPKRRSSSRFIKRKKFDDELVESSLAKSSTRAKGASGVEPGRCSGSEPSSSEKKKVSKAPSTPVPPSPAPAPGLTKRVKKSKQPLQVTKDLGRWKPADDLLLINAVLQTNDLTSVHLGVKFSCRFTLREVQERWYALLYDPVISKLACQAMRQLHPEAIAAIQSKALFSKAEEQLLSKVGSTSQPTLETFQDLLHRHPDAFYLARTAKALQAHWQLMKQYYLLEDQTVQPLPKGDQVLNFSDAEDLIDDSKLKDMRDEVLEHELMVADRRQKREIRQLEQELHKWQVLVDSITGMSSPDFDNQTLAVLRGRMVRYLMRSREITLGRATKDNQIDVDLSLEGPAWKISRKQGVIKLKNNGDFFIANEGRRPIYIDGRPVLCGSKWRLSNNSVVEIASLRFVFLINQDLIALIRAEAAKITPQ
uniprot
proteins
function
FUNCTION: Modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus (PubMed:11948183). As part of the NSL complex, may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. May also be an inhibitor of TERT telomerase activity (PubMed:15044100). Binds to G-quadruplex structures in mRNA (PubMed:16571602). Binds to RNA homomer poly(G) and poly(U) (PubMed:16571602). Maintains RHEB at the lysosome in its active GTP-bound form and prevents its interaction with the mTORC1 complex inhibitor TSC2, ensuring activation of the mTORC1 complex by RHEB (PubMed:25816988). Stabilizes the minus ends of kinetochore fibers by protecting them from depolymerization, ensuring functional spindle assembly during mitosis (PubMed:22081094, PubMed:27192185). Following phosphorylation by TTK/MPS1, enhances recruitment of KIF2A to the minus ends of mitotic spindle microtubules which promotes chromosome alignment (PubMed:30785839). Regulates the morphology of microtubule minus ends in mitotic spindle by maintaining them in a closed conformation characterized by the presence of an electron-dense cap (PubMed:36350698). Regulates G2/M transition and spindle assembly during oocyte meiosis (By similarity). Mediates histone modifications and transcriptional regulation in germinal vesicle oocytes which are required for meiotic progression (By similarity). Also regulates microtubule nucleation and spindle assembly by activating aurora kinases during oocyte meiosis (By similarity). Contributes to the establishment of centriolar satellites and also plays a role in primary cilium formation by recruiting TTBK2 to the mother centriole which is necessary for removal of the CP110 cap from the mother centriole, an early step in ciliogenesis (PubMed:27263857). Required for epiblast development during early embryogenesis (By similarity). Essential for cell viability (PubMed:16547491). {ECO:0000250|UniProtKB:Q99L90, ECO:0000269|PubMed:11948183, ECO:0000269|PubMed:15044100, ECO:0000269|PubMed:16547491, ECO:0000269|PubMed:16571602, ECO:0000269|PubMed:20018852, ECO:0000269|PubMed:22081094, ECO:0000269|PubMed:25816988, ECO:0000269|PubMed:27192185, ECO:0000269|PubMed:27263857, ECO:0000269|PubMed:30785839, ECO:0000269|PubMed:36350698}.
uniprot
proteins
mass_dalton
51803
uniprot
proteins
mutagenesis
MUTAGEN 35..36; /note="SS->AA: Reduces phosphorylation by AURKA. Does not rescue the mitotic defect seen in MCRS1-silenced cells. Does not affect localization to the spindle poles."; /evidence="ECO:0000269|PubMed:27192185"; MUTAGEN 35..36; /note="SS->EE: Phosphomimetic mutant; shows a more severe mitotic defect than MCRS1-silenced cells. Does not affect localization to the spindle poles."; /evidence="ECO:0000269|PubMed:27192185"; MUTAGEN 64..65; /note="SS->AA: Reduces phosphorylation by TTK. Cells show a mitotic defect and remain longer in mitosis."; /evidence="ECO:0000269|PubMed:30785839"; MUTAGEN 65; /note="S->A: Exhibits mitotic defect. Does not rescue the misaligned chromosome phenotype seen in MCRS1-silenced cells. Does not affect localization to the nucleus and nucleolus during interphase or to the spindle poles during mitosis."; /evidence="ECO:0000269|PubMed:30785839"; MUTAGEN 65; /note="S->D: Exhibits normal mitotic timing. Rescues the misaligned chromosome phenotype seen in MCRS1-silenced cells."; /evidence="ECO:0000269|PubMed:30785839
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated by UBR5 when not assembled in the INO80 complex, leading to its degradation: UBR5 recognizes and binds a degron that is not accessible when MCRS1 is part of the INO80 complex. {ECO:0000269|PubMed:37478862}.; PTM: Phosphorylated by AURKA on Ser-35 and/or Ser-36 during mitosis which is required for kinetochore fiber assembly and mitotic progression but not for spindle localization or for chromosome-induced microtubule aster formation (PubMed:27192185). Also phosphorylated by AURKA on Ser-85 and/or Ser-87 (PubMed:27192185). Phosphorylated by TTK/MPS1 which enhances recruitment of KIF2A to the minus end of spindle microtubules and facilitates precise chromosome segregation (PubMed:30785839). {ECO:0000269|PubMed:27192185, ECO:0000269|PubMed:30785839}.
uniprot
proteins
pubmed
11948183
uniprot
proteins
pubmed
15044100
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15960975
uniprot
proteins
pubmed
16230350
uniprot
proteins
pubmed
16541075
uniprot
proteins
pubmed
16547491
uniprot
proteins
pubmed
16571602
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
16964243
uniprot
proteins
pubmed
17014843
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
18922472
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19608861
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20018852
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21303910
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
22081094
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
25816988
uniprot
proteins
pubmed
27192185
uniprot
proteins
pubmed
27263857
uniprot
proteins
pubmed
30785839
uniprot
proteins
pubmed
36350698
uniprot
proteins
pubmed
37478862
uniprot
proteins
pubmed
9654073
uniprot
proteins
pubmed
9765390
uniprot
proteins
sequence_length
462
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15044100, ECO:0000269|PubMed:16547491, ECO:0000269|PubMed:16571602, ECO:0000269|PubMed:18922472, ECO:0000269|PubMed:20018852, ECO:0000269|PubMed:22081094, ECO:0000269|PubMed:30785839}. Nucleus, nucleolus {ECO:0000269|PubMed:16571602, ECO:0000269|PubMed:9654073}. Cytoplasm {ECO:0000269|PubMed:16571602, ECO:0000269|PubMed:30785839}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:16547491}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:16547491, ECO:0000269|PubMed:22081094, ECO:0000269|PubMed:27192185, ECO:0000269|PubMed:30785839}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:22081094}. Chromosome {ECO:0000250|UniProtKB:Q99L90}. Lysosome {ECO:0000269|PubMed:25816988}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite {ECO:0000269|PubMed:27263857}. Note=Predominantly concentrated in the nucleus but also localizes to the centrosome (PubMed:16547491). Detected on the spindle poles during mitosis from prometaphase to telophase (PubMed:16547491). Found in microspherules in the nucleolus (PubMed:9654073). Localizes to lysosomes under high amino acid concentration conditions (PubMed:25816988). Localizes to the minus ends of kinetochore fibers and chromosomal microtubules (PubMed:22081094). Present in the nucleus of germinal vesicle oocytes and associates with spindles poles and chromosomes after germinal vesicle breakdown (By similarity). {ECO:0000250|UniProtKB:Q99L90, ECO:0000269|PubMed:16547491, ECO:0000269|PubMed:22081094, ECO:0000269|PubMed:25816988, ECO:0000269|PubMed:9654073}.
uniprot
proteins