GABARAPL2
entrez
11345
canonical
uniprot
P60520
canonical
ensembl
ENSG00000034713
resolver
ensp
ENSP00000037243
resolver
enst
ENST00000037243
resolver
genesymbol
GABARAPL2
resolver
genesymbol_syn
FLC3A
resolver
genesymbol_syn
GEF2
resolver
hgnc
HGNC:13291
resolver
uniprot
O08765
resolver
uniprot
Q6FG91
resolver
uniprot
Q9DCP8
resolver
uniprot
Q9UQF7
resolver
uniprot_entry
GBRL2_HUMAN
resolver
uniprot_sec
O08765
resolver
uniprot_sec
Q6FG91
resolver
uniprot_sec
Q9DCP8
resolver
uniprot_sec
Q9UQF7
resolver
amino_acid_sequence
MKWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGFLYVAYSGENTFGF
uniprot
proteins
function
FUNCTION: Ubiquitin-like modifier involved in intra-Golgi traffic (By similarity). Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation (By similarity). It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy (PubMed:20418806, PubMed:23209295). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:20418806, PubMed:23209295). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295). {ECO:0000250|UniProtKB:P60519, ECO:0000269|PubMed:20418806, ECO:0000269|PubMed:23209295}.
uniprot
proteins
mass_dalton
13667
uniprot
proteins
mutagenesis
MUTAGEN 47; /note="R->A: Strongly reduced interaction with UBA5."; /evidence="ECO:0000269|PubMed:30990354"; MUTAGEN 87..88; /note="SS->AA: Impaired phosphorylation by TBK1."; /evidence="ECO:0000269|PubMed:31709703"; MUTAGEN 87..88; /note="SS->DD: Phospho-mimetic mutant; impaired interaction with ATG4 proteins, preventing cleavage at the C-terminus, conjugation to phosphatidylethanolamine."; /evidence="ECO:0000269|PubMed:31709703"; MUTAGEN 88; /note="S->D: Phospho-mimetic mutant; abolished localization to autophagosomes."; /evidence="ECO:0000269|PubMed:31709703"; MUTAGEN 116; /note="G->A: Impairs localization at the autophagosomal membrane."; /evidence="ECO:0000269|PubMed:15169837
uniprot
proteins
post_translational_modification
PTM: The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, GABARAPL2-I (PubMed:15169837, PubMed:20818167, PubMed:30661429, PubMed:31709703). The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, GABARAPL2-II (PubMed:15169837, PubMed:31709703). During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989). ATG4 proteins also mediate the delipidation of PE-conjugated forms required for GABARAPL2 recycling when autophagosomes fuse with lysosomes (PubMed:29458288, PubMed:31709703, PubMed:33909989). In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989). ATG4B constitutes the major protein for proteolytic activation (PubMed:30661429). ATG4D is the main enzyme for delipidation activity (By similarity). {ECO:0000250|UniProtKB:P60521, ECO:0000269|PubMed:15169837, ECO:0000269|PubMed:20818167, ECO:0000269|PubMed:29458288, ECO:0000269|PubMed:30661429, ECO:0000269|PubMed:31709703, ECO:0000269|PubMed:33909989}.; PTM: (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:31722778). RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989). {ECO:0000269|PubMed:31722778, ECO:0000269|PubMed:33909989}.; PTM: Phosphorylation at Ser-87 and Ser-88 by TBK1 prevents interaction with ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) (PubMed:31709703). Phosphorylation by TBK1 on autophagosomes prevents their delipidation by ATG4 and premature removal from nascent autophagosomes (PubMed:31709703). {ECO:0000269|PubMed:31709703}.
uniprot
proteins
protein_family
ATG8 family
uniprot
proteins
pubmed
11096062
uniprot
proteins
pubmed
11146101
uniprot
proteins
pubmed
11414770
uniprot
proteins
pubmed
11825910
uniprot
proteins
pubmed
12507496
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15169837
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
17525332
uniprot
proteins
pubmed
17580304
uniprot
proteins
pubmed
19056683
uniprot
proteins
pubmed
19608861
uniprot
proteins
pubmed
20418806
uniprot
proteins
pubmed
20562859
uniprot
proteins
pubmed
20818167
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21383079
uniprot
proteins
pubmed
22311637
uniprot
proteins
pubmed
22354992
uniprot
proteins
pubmed
22421968
uniprot
proteins
pubmed
22470510
uniprot
proteins
pubmed
23043107
uniprot
proteins
pubmed
23209295
uniprot
proteins
pubmed
24089205
uniprot
proteins
pubmed
24668264
uniprot
proteins
pubmed
25127057
uniprot
proteins
pubmed
25684205
uniprot
proteins
pubmed
26347139
uniprot
proteins
pubmed
26929408
uniprot
proteins
pubmed
29420192
uniprot
proteins
pubmed
29458288
uniprot
proteins
pubmed
30661429
uniprot
proteins
pubmed
30990354
uniprot
proteins
pubmed
31709703
uniprot
proteins
pubmed
31722778
uniprot
proteins
pubmed
32315611
uniprot
proteins
pubmed
33909989
uniprot
proteins
pubmed
34338405
uniprot
proteins
pubmed
39481378
uniprot
proteins
sequence_length
117
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome {ECO:0000269|PubMed:12507496, ECO:0000269|PubMed:15169837, ECO:0000269|PubMed:17580304, ECO:0000269|PubMed:19056683, ECO:0000269|PubMed:22311637, ECO:0000269|PubMed:31709703}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:30990354}. Golgi apparatus {ECO:0000250|UniProtKB:P60519}.
uniprot
proteins