Fbxo5
entrez
67141
canonical
uniprot
Q7TSG3
canonical
ensembl
ENSMUSG00000019773
resolver
ensp
ENSMUSP00000019907
resolver
enst
ENSMUST00000019907
resolver
genesymbol
Fbxo5
resolver
genesymbol_syn
Emi1
resolver
uniprot_entry
FBX5_MOUSE
resolver
amino_acid_sequence
MSRRTCSDLRRPSSCPCRLGARTTVDGCKEESPVLSVTMKCFNCNPDLSELEVVKPEDSGIEASYSPVCLEPSCNDCVRNHERLSFIDSPIVGHDNKENQRVQNTLDSSNETEELEASRLYEDSGYSSFTQSDRDDGILILENFRNSPQARLLPSQSPDQHPNKTLLPVLHFERVVCSTLKKNGKRNPKVDREMLKEVIASGNFRLQNIIGKKMGLEHLDILAELSRRGFVHLLANILTKLSGMDLVNLSKVSRIWKKILENNKGAFQLYSKTMQRVIESSKLSLHATTRGYVVGRAALTCVQKSSTWAPPKKDVQIKSSSQRGQRVSTYSRHNEFVEVAKTLKNNESLKACVRCNFPAKYDHYLERAVCKRESCQFEYCTKCLCAYHNNKDCLNGKILKASCKVGPLPGTKKSKKNLQRL
uniprot
proteins
function
FUNCTION: Regulator of APC activity during mitotic and meiotic cell cycle (PubMed:15526037, PubMed:16809773, PubMed:17190794). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed:16809773). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase. Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment. As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box co-receptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities. Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (By similarity). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (PubMed:15526037). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (PubMed:17190794). Promotes migration and osteogenic differentiation of mesenchymal stem cells (By similarity). {ECO:0000250|UniProtKB:Q9UKT4, ECO:0000269|PubMed:15526037, ECO:0000269|PubMed:16809773, ECO:0000269|PubMed:17190794}.
uniprot
proteins
mass_dalton
47508
uniprot
proteins
mutagenesis
MUTAGEN 284; /note="S->A: Decreases phosphorylation by RPS6KA2."; /evidence="ECO:0000269|PubMed:15526037"; MUTAGEN 289; /note="T->A: Decreases phosphorylation by RPS6KA2."; /evidence="ECO:0000269|PubMed:15526037"; MUTAGEN 342; /note="T->A: Does not affect phosphorylation by RPS6KA2."; /evidence="ECO:0000269|PubMed:15526037"; MUTAGEN 348; /note="S->A: Does not affect phosphorylation by RPS6KA2."; /evidence="ECO:0000269|PubMed:15526037
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination.
uniprot
proteins
post_translational_modification
PTM: Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression (By similarity). Phosphorylated by RPS6KA2; increases and stabilizes interaction with CDC20 (PubMed:15526037). {ECO:0000250|UniProtKB:Q9UKT4, ECO:0000269|PubMed:15526037}.; PTM: Ubiquitinated by the SCF(BTRC) complex following phosphorylation by PLK1. Undergoes both 'Lys-11' and 'Lys-48'-linked polyubiquitination by APC-FZR1 complex leading to degradation during G1 phase by the proteasome (By similarity). Degraded through the SCF(BTRC) complex; degradation occurs during oocyte maturation, between germinal vesicle breakdown (GVBD) and meiosis I, and is required for the meiosis I-meiosis II transition (PubMed:17190794). {ECO:0000250|UniProtKB:Q9UKT4, ECO:0000269|PubMed:17190794}.
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15526037
uniprot
proteins
pubmed
16809773
uniprot
proteins
pubmed
17190794
uniprot
proteins
pubmed
17875940
uniprot
proteins
pubmed
21183079
uniprot
proteins
sequence_length
421
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UKT4}. Cytoplasm {ECO:0000269|PubMed:15526037}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:15526037}. Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle. {ECO:0000250|UniProtKB:Q9UKT4}.
uniprot
proteins