Q5I0K7
uniprot
Q5I0K7
canonical
amino_acid_sequence
MKRAFVTVGTTSFDDLVARVVANDTVQILKSLGYNHLVLQIGRGTVVPEPFSTEPFTLDVYRYKESLKEDLQQADLVISHAGAGSCLESLEKGKPLVVVVNEKLMNNHQFELAKQLHKEGHLFYCTCSMLPGLLQSMDLSTLKCYPPGQPEKFSAFLDKVVGLQK
uniprot
proteins
ec_number
2.4.1.141
uniprot
proteins
function
FUNCTION: Catalytic subunit of the UDP-N-acetylglucosamine transferase complex that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. On the cytoplasmic face of the endoplasmic reticulum, the dimeric ALG13/ALG14 complex catalyzes the second step of dolichol pyrophosphate biosynthesis, transferring a beta1,4-linked N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to GlcNAc-pyrophosphatedolichol (Gn-PDol) to produce N,N'-diacetylchitobiosyl diphosphodolichol. N,N'-diacetylchitobiosyl diphosphodolichol is a substrate for ALG1, the following enzyme in the biosynthetic pathway. {ECO:0000250|UniProtKB:Q9NP73}.
uniprot
proteins
mass_dalton
18329
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein glycosylation. {ECO:0000250|UniProtKB:Q9NP73}.
uniprot
proteins
protein_family
Glycosyltransferase 28 family
uniprot
proteins
pubmed
15489334
uniprot
proteins
sequence_length
165
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9NP73}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9NP73}. Note=Recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14. {ECO:0000250|UniProtKB:Q9NP73}.
uniprot
proteins