O35254
uniprot
O35254
canonical
amino_acid_sequence
MGLGASSEQPAGGEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEHVWHVLDVEPSSPAALAGLRPYTDYIVGSDQILQESEDFFTLIESHEGKPLKLMVYNSESDSCREVTVTPNAAWGGEGSLGCGIGYGYLHRIPTQPSSQYKKPPSASSPGTPAKTPQPNAFPLGAPPPWPIPQDSSGPELGSRQSDYMEALPQVPGGFMEEQLPGPGSPGHGTADYGGCLHSMEIPLQPPPPVQRVMDPGFLDVSGMSLLDSNNTSVCPSLSSSSLLTPTAVSALGPEDIGSSSSSHERGGEATWSGSEFEISFPDSPGSQAQVDHLPRLTLPDGLTSAASPEEGLSAELLEAQTEEPAHTASLDCMAQTEGPAGQVQAAPDPEPGLCEGPW
uniprot
proteins
function
FUNCTION: Key structural protein of the Golgi apparatus (By similarity). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (By similarity). Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (By similarity). However, other studies suggest that GORASP1 plays an important role in assembly and membrane stacking of the cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (PubMed:12839990, PubMed:23940043, PubMed:9346242). Caspase-mediated cleavage of GORASP1 is required for fragmentation of the Golgi during apoptosis (PubMed:11815631, PubMed:12015985). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity). {ECO:0000250|UniProtKB:Q91X51, ECO:0000250|UniProtKB:Q9BQQ3, ECO:0000269|PubMed:11815631, ECO:0000269|PubMed:12015985, ECO:0000269|PubMed:12839990, ECO:0000269|PubMed:23940043, ECO:0000269|PubMed:9346242}.
uniprot
proteins
mass_dalton
47673
uniprot
proteins
mutagenesis
MUTAGEN 54; /note="L->A: Strongly reduced ability to promote Golgi stack reassembly."; /evidence="ECO:0000269|PubMed:23940043"; MUTAGEN 149; /note="F->A: Strongly reduced ability to promote Golgi stack reassembly."; /evidence="ECO:0000269|PubMed:23940043"; MUTAGEN 209; /note="Y->A: Strongly reduced ability to promote Golgi stack reassembly."; /evidence="ECO:0000269|PubMed:23940043"; MUTAGEN 320; /note="D->A: Blocks the caspase-mediated cleavage."; /evidence="ECO:0000269|PubMed:11815631"; MUTAGEN 375; /note="D->A: Blocks the caspase-mediated cleavage."; /evidence="ECO:0000269|PubMed:11815631"; MUTAGEN 393; /note="D->A: Blocks the caspase-mediated cleavage."; /evidence="ECO:0000269|PubMed:11815631
uniprot
proteins
post_translational_modification
PTM: Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking. {ECO:0000269|PubMed:11050165, ECO:0000269|PubMed:12839990, ECO:0000269|PubMed:9346242}.; PTM: Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis. {ECO:0000269|PubMed:11815631}.; PTM: Myristoylated. {ECO:0000269|PubMed:9346242}.
uniprot
proteins
protein_family
GORASP family
uniprot
proteins
pubmed
11050165
uniprot
proteins
pubmed
11706049
uniprot
proteins
pubmed
11739401
uniprot
proteins
pubmed
11739402
uniprot
proteins
pubmed
11815631
uniprot
proteins
pubmed
12015985
uniprot
proteins
pubmed
12839990
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
23940043
uniprot
proteins
pubmed
9346242
uniprot
proteins
sequence_length
451
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane {ECO:0000269|PubMed:11706049, ECO:0000269|PubMed:11739401, ECO:0000269|PubMed:12015985, ECO:0000269|PubMed:12839990, ECO:0000269|PubMed:9346242}; Peripheral membrane protein {ECO:0000269|PubMed:9346242}; Cytoplasmic side {ECO:0000269|PubMed:9346242}.
uniprot
proteins