Alkbh2
entrez
231642
canonical
uniprot
Q6P6J4
canonical
ensembl
ENSMUSG00000044339
resolver
ensp
ENSMUSP00000056043
resolver
ensp
ENSMUSP00000107898
resolver
ensp
ENSMUSP00000242918
resolver
ensp
ENSMUSP00000242925
resolver
ensp
ENSMUSP00000327716
resolver
enst
ENSMUST00000053657
resolver
enst
ENSMUST00000112279
resolver
genesymbol
Alkbh2
resolver
genesymbol_syn
Abh2
resolver
uniprot
Q8C591
resolver
uniprot_entry
ALKB2_MOUSE
resolver
uniprot_sec
Q8C591
resolver
activity_regulation
ACTIVITY REGULATION: Activated by magnesium ions. {ECO:0000269|PubMed:16642038}.
uniprot
proteins
amino_acid_sequence
MDKFLVRPDLRDLQGGGEEPAPTGGASGDLKSPDWRHLRAEGLSCDYTVLFGKAEADKIFRELEQEVEYFTGALAKVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLTPKPWVPVLERVRDRVCEVTGQTFNFVLVNRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFIFRHKDSRGKRPRRTVEVVRLQLAHGSLLMMNPPTNTHWYHSLPIRKRVLAPRVNLTFRKILPTKK
uniprot
proteins
ec_number
1.14.11.33
uniprot
proteins
function
FUNCTION: Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA (PubMed:16174769, PubMed:16642038, PubMed:18519673). Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis (By similarity) (PubMed:16174769, PubMed:16642038, PubMed:18519673). Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine (By similarity). Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription (By similarity). In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication (By similarity). {ECO:0000250|UniProtKB:Q6NS38, ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:16642038, ECO:0000269|PubMed:18519673}.
uniprot
proteins
mass_dalton
27129
uniprot
proteins
mutagenesis
MUTAGEN 151; /note="D->A: Loss of activity."; /evidence="ECO:0000269|PubMed:16174769"; MUTAGEN 214; /note="H->A: Reduces activity."; /evidence="ECO:0000269|PubMed:16174769
uniprot
proteins
protein_family
AlkB family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16174769
uniprot
proteins
pubmed
16642038
uniprot
proteins
pubmed
18519673
uniprot
proteins
sequence_length
239
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16642038, ECO:0000269|PubMed:18519673}. Nucleus, nucleolus {ECO:0000250|UniProtKB:Q6NS38}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q6NS38}. Note=Relocates to the replication foci during S-phase. {ECO:0000250|UniProtKB:Q6NS38}.
uniprot
proteins