Cps1
entrez
497840
canonical
uniprot
P07756
canonical
ensembl
ENSRNOG00055010377
resolver
ensembl
ENSRNOG00060019455
resolver
ensembl
ENSRNOG00065028982
resolver
ensp
ENSRNOP00055014140
resolver
ensp
ENSRNOP00060027534
resolver
ensp
ENSRNOP00065041155
resolver
enst
ENSRNOT00055017561
resolver
enst
ENSRNOT00060033638
resolver
enst
ENSRNOT00065050086
resolver
genesymbol
Cps1
resolver
uniprot_entry
CPSM_RAT
resolver
activity_regulation
ACTIVITY REGULATION: Requires N-acetyl-L-glutamate (NAG) as an allosteric activator. N-acetyl-L-beta-phenylglutamate (Phe-NAG) can also activate CPSase I, but with an activation constant that is 2-fold higher than that for NAG. {ECO:0000269|PubMed:19754428}.
uniprot
proteins
amino_acid_sequence
MTRILTACKVVKTLKSGFGLANVTSKRQWDFSRPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYSEALTDPAYKGQILTMANPIIGNGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSHDYNHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVKVFGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMDYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFDSFFSLIKKGKGTTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLMDLGTKAFAMTNQILVERSVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLRKELSEPSSTRIYAIAKALENNMSLDEIVKLTSIDKWFLYKMRDILNMDKTLKGLNSESVTEETLRQAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFDEHGIMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACNGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFANSVGYPCLLRPSYVLSGSAMNVVFSEDEMKRFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKEGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGVDFIDVATKVMIGESVDEKHLPTLEQPIIPSDYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVQKARTVDSKSLFHYRQYSAGKAA
uniprot
proteins
ec_number
6.3.4.16
uniprot
proteins
function
FUNCTION: Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
uniprot
proteins
mass_dalton
164580
uniprot
proteins
mutagenesis
MUTAGEN 1391; /note="T->V: 400-fold increase in the activation constant of NAG. 3-fold decrease in the reaction rate at saturation of NAG."; /evidence="ECO:0000269|PubMed:19754428"; MUTAGEN 1394; /note="T->A: 900-fold increase in the activation constant of NAG. 3-fold decrease in the reaction rate at saturation of NAG."; /evidence="ECO:0000269|PubMed:19754428"; MUTAGEN 1410; /note="W->K: 60-fold increase in the activation constant of NAG."; /evidence="ECO:0000269|PubMed:19754428"; MUTAGEN 1437; /note="N->D: 70-fold increase in the activation constant of NAG."; /evidence="ECO:0000269|PubMed:19754428"; MUTAGEN 1440; /note="N->D: 110-fold increase in the activation constant of NAG. Modifies the specificity for the activator: Binds Phe-NAG considerably better than NAG."; /evidence="ECO:0000269|PubMed:19754428
uniprot
proteins
post_translational_modification
PTM: 50% of the mature protein that was isolated had Leu-39 as its N-terminal residue and 50% had Ser-40 suggesting two adjacent processing sites. However, the possibility of proteolytic removal of Leu-39 during the isolation of the enzyme cannot be excluded. Undergoes proteolytic cleavage in the C-terminal region corresponding to the loss of approximately 12 AA residues from the C-terminus (PubMed:23649895). {ECO:0000269|PubMed:23649895}.; PTM: Succinylated at Lys-287 and Lys-1291. Desuccinylated at Lys-1291 by SIRT5, leading to activation (By similarity). {ECO:0000250|UniProtKB:Q8C196}.; PTM: Glutarylated. Glutarylation levels increase during fasting. Deglutarylated by SIRT5 at Lys-55, Lys-219, Lys-412, Lys-889, Lys-892, Lys-915, Lys-1360 and Lys-1486, leading to activation. {ECO:0000250|UniProtKB:P31327}.
uniprot
proteins
pubmed
19754428
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
23649895
uniprot
proteins
pubmed
24098488
uniprot
proteins
pubmed
2991241
uniprot
proteins
pubmed
3038878
uniprot
proteins
sequence_length
1500
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion. Nucleus, nucleolus {ECO:0000250|UniProtKB:P31327}. Cell membrane {ECO:0000250|UniProtKB:Q8C196}; Peripheral membrane protein {ECO:0000305}; Extracellular side {ECO:0000250|UniProtKB:Q8C196}. Note=Localizes to the cell surface of hepatocytes. {ECO:0000250|UniProtKB:Q8C196}.
uniprot
proteins