Slc12a2
entrez
20496
canonical
uniprot
P55012
canonical
ensembl
ENSMUSG00000024597
resolver
ensp
ENSMUSP00000111023
resolver
ensp
ENSMUSP00000183715
resolver
enst
ENSMUST00000115366
resolver
genesymbol
Slc12a2
resolver
genesymbol_syn
Nkcc1
resolver
uniprot
E9PYU3
resolver
uniprot
E9QM38
resolver
uniprot_entry
E9QM38_MOUSE
resolver
uniprot_entry
S12A2_MOUSE
resolver
uniprot_sec
E9PYU3
resolver
activity_regulation
ACTIVITY REGULATION: Activated following phosphorylation by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4). Inhibited by bumetanide and furosemide (PubMed:36306358). {ECO:0000269|PubMed:14563843, ECO:0000269|PubMed:16382158, ECO:0000269|PubMed:21972418, ECO:0000269|PubMed:36306358}.
uniprot
proteins
amino_acid_sequence
MEPGPARPRLAPAARPGWGRAAGCRRRGGPARHGRASGQEDATTAGRQAGGGVRGEGTPAAGDGLGRPLGPTPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFVDPAASSSADDSLSDAAGVGGDGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTFYS
uniprot
proteins
function
FUNCTION: Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane (PubMed:14563843, PubMed:32658972, PubMed:36306358). Plays a vital role in the regulation of ionic balance and cell volume (By similarity). {ECO:0000250|UniProtKB:P55011, ECO:0000269|PubMed:14563843, ECO:0000269|PubMed:32658972, ECO:0000269|PubMed:36306358}.
uniprot
proteins
mass_dalton
131034
uniprot
proteins
mutagenesis
MUTAGEN 78; /note="F->A: Abolished interaction with STK39/SPAK and subsequent phosphorylation and activation."; /evidence="ECO:0000269|PubMed:14563843"; MUTAGEN 135..136; /note="RV->AA: Abolished interaction with STK39/SPAK and subsequent phosphorylation and activation."; /evidence="ECO:0000269|PubMed:14563843"; MUTAGEN 320; /note="A->V: Reduced potassium ion transmembrane transport."; /evidence="ECO:0000269|PubMed:32658972"; MUTAGEN 369; /note="N->I: Reduced potassium ion transmembrane transport."; /evidence="ECO:0000269|PubMed:32658972"; MUTAGEN 372; /note="A->L: Reduced potassium ion transmembrane transport."; /evidence="ECO:0000269|PubMed:32658972"; MUTAGEN 403; /note="R->Q: Reduced potassium ion transmembrane transport."; /evidence="ECO:0000269|PubMed:32658972"; MUTAGEN 794; /note="R->A: Decrease in K(+) influx and reduced sensitivity to inhibitor bumetanide."; /evidence="ECO:0000269|PubMed:36306358"; MUTAGEN 885..1205; /note="Missing: Reduced potassium ion transmembrane transport."; /evidence="ECO:0000269|PubMed:32658972"; MUTAGEN 973; /note="E->K: Reduced potassium ion transmembrane transport."; /evidence="ECO:0000269|PubMed:32658972
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Thr-196, Thr-200 and Thr-205 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity. {ECO:0000250|UniProtKB:P55011}.
uniprot
proteins
protein_family
SLC12A transporter family
uniprot
proteins
pubmed
14563843
uniprot
proteins
pubmed
16382158
uniprot
proteins
pubmed
19144319
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
19656770
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21972418
uniprot
proteins
pubmed
32294086
uniprot
proteins
pubmed
32658972
uniprot
proteins
pubmed
36306358
uniprot
proteins
pubmed
7929272
uniprot
proteins
sequence_length
1205
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Basolateral cell membrane {ECO:0000250|UniProtKB:A0A0G2KTI4}; Multi-pass membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 280..309; /note="Discontinuously helical; Name=1"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 310..329; /note="Helical; Name=2"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 361..388; /note="Helical; Name=3"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 399..422; /note="Helical; Name=4"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 426..447; /note="Helical; Name=5"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 480..497; /note="Discontinuously helical; Name=6"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 513..534; /note="Helical; Name=7"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 592..616; /note="Helical; Name=8"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 645..665; /note="Helical; Name=9"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 666..684; /note="Helical; Name=10"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 708..725; /note="Helical; Name=11"; /evidence="ECO:0000250|UniProtKB:P55011"; TRANSMEM 726..738; /note="Helical; Name=12"; /evidence="ECO:0000250|UniProtKB:P55011
uniprot
proteins