P35763
uniprot
P35763
canonical
amino_acid_sequence
MAAYLFLLGLFLLLPRPVPAPCYTATRSECKQNHKFVPGVWAAGEGVDVTTLRRSSSFPVNTGKFLRPDRTCTLCKNALMNDGIQRLPVAIAHWRPHGSHCQRNVATTKVSSTEGVAREAAANINNDWRAGLDVNPKPEANVHVSVAGSHSKIANFAAEKAHQDQYNFNTDTVECRMYSFRLAQKPPLHPDFRKALKNLPHNFNSSTEHAYRRLISSYGTHFITAVDLGGRVSVLTALRTCQLTLDGLTADEVGDCLSVEAQVSIGAQASVSSEYKACEEKKKQHKIATSFHQTYRERHVEVLGGPLDSSNDLLFGNQATPEHFSTWIASLPTRPDVVDYSLEPLHILLEDSDPKREALRQAISHYVMSRARWRDCNRPCRAGQHKSSRDSCQCVCQDSNVTNQDCCPRQRGLAKLMVRNFQAKGLWGDYITSTDAYLKVFFGGQEIRTGVVWNNNHPSWSDKMDFGNVLLSTGGPLRVQVWDADNGWDDDLLGTCDKSPKSGFHEVNCPLNHGSIKFIYQANCLPDLTGETCLEYAPQGLLGDPRGNRSGAVW
uniprot
proteins
function
FUNCTION: Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:2809217). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (By similarity). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (By similarity). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (By similarity). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity). {ECO:0000250|UniProtKB:P10820, ECO:0000250|UniProtKB:P14222, ECO:0000269|PubMed:2809217}.
uniprot
proteins
mass_dalton
61513
uniprot
proteins
post_translational_modification
PTM: N-glycosylated. {ECO:0000250|UniProtKB:P10820}.
uniprot
proteins
protein_family
Complement C6/C7/C8/C9 family
uniprot
proteins
pubmed
2809217
uniprot
proteins
sequence_length
554
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytolytic granule {ECO:0000269|PubMed:2809217}. Secreted {ECO:0000269|PubMed:2809217}. Cell membrane {ECO:0000269|PubMed:2809217}; Multi-pass membrane protein {ECO:0000269|PubMed:2809217}. Endosome lumen {ECO:0000250|UniProtKB:P14222}. Note=Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes (By similarity). {ECO:0000250|UniProtKB:P14222}.
uniprot
proteins
transmembrane_region
TRANSMEM 128..148; /note="Beta stranded; Name=CH1"; /evidence="ECO:0000250|UniProtKB:P10820"; TRANSMEM 256..278; /note="Beta stranded; Name=CH2"; /evidence="ECO:0000250|UniProtKB:P10820
uniprot
proteins