Qtrt1
entrez
60507
canonical
uniprot
Q9JMA2
canonical
ensembl
ENSMUSG00000002825
resolver
ensp
ENSMUSP00000002902
resolver
ensp
ENSMUSP00000339681
resolver
enst
ENSMUST00000002902
resolver
genesymbol
Qtrt1
resolver
genesymbol_syn
Tgt
resolver
genesymbol_syn
Tgut
resolver
uniprot
Q80VS3
resolver
uniprot_entry
TGT_MOUSE
resolver
uniprot_sec
Q80VS3
resolver
amino_acid_sequence
MAAVGSPGSLESAPRIMRLVAECSRSGARAGELRLPHGTVATPVFMPVGTQATMKGITTEQLDSLGCRICLGNTYHLGLRPGPELIRKAQGLHGFMNWPHNLLTDSGGFQMVSLFSLSEVTEEGVHFRSPYDGEETLLSPERSVEIQNALGSDIIMQLDHVVSSTVTGPLVEEAMHRSVRWLDRCIAAHKHPDKQNLFAIIQGGLNADLRTTCLKEMTKRDVPGFAIGGLSGGESKAQFWKMVALSTSMLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVYPTRTARFGSALVPTGNLQLKKKQYAKDFSPINPECPCPTCQTHSRAFLHALLHSDNTTALHHLTVHNIAYQLQLLSAVRSSILEQRFPDFVRNFMRTMYGDHSLCPAWAVEALASVGIMLT
uniprot
proteins
ec_number
2.4.2.64
uniprot
proteins
function
FUNCTION: Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:19414587, PubMed:29862811, PubMed:35815944, PubMed:37609797). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity). Modification of cytoplasmic tRNAs with queuosine controls the elongation speed of cognate codons, thereby ensuring the correct folding of nascent proteins to maintain proteome integrity (PubMed:37609797). {ECO:0000255|HAMAP-Rule:MF_03218, ECO:0000269|PubMed:19414587, ECO:0000269|PubMed:29862811, ECO:0000269|PubMed:35815944, ECO:0000269|PubMed:37609797}.
uniprot
proteins
mass_dalton
44093
uniprot
proteins
protein_family
Queuine tRNA-ribosyltransferase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19414587
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
29862811
uniprot
proteins
pubmed
35815944
uniprot
proteins
pubmed
37609797
uniprot
proteins
sequence_length
403
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03218, ECO:0000269|PubMed:19414587}. Mitochondrion outer membrane {ECO:0000255|HAMAP-Rule:MF_03218, ECO:0000269|PubMed:19414587}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03218, ECO:0000305|PubMed:19414587}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03218, ECO:0000269|PubMed:19414587}. Nucleus {ECO:0000269|PubMed:19414587}. Note=Weakly associates with mitochondria, possibly via QTRT2. {ECO:0000255|HAMAP-Rule:MF_03218, ECO:0000269|PubMed:19414587}.
uniprot
proteins