METTL14
entrez
57721
canonical
uniprot
Q9HCE5
canonical
ensembl
ENSG00000145388
resolver
ensp
ENSP00000373474
resolver
ensp
ENSP00000685201
resolver
enst
ENST00000388822
resolver
genesymbol
METTL14
resolver
genesymbol_syn
KIAA1627
resolver
hgnc
HGNC:29330
resolver
uniprot
A6NIG1
resolver
uniprot
Q969V2
resolver
uniprot_entry
MET14_HUMAN
resolver
uniprot_sec
A6NIG1
resolver
uniprot_sec
Q969V2
resolver
amino_acid_sequence
MDSRLQEIRERQKLRRQLLAQQLGAESADSIGAVLNSKDEQREIAETRETCRASYDTSAPNAKRKYLDEGETDEDKMEEYKDELEMQQDEENLPYEEEIYKDSSTFLKGTQSLNPHNDYCQHFVDTGHRPQNFIRDVGLADRFEEYPKLRELIRLKDELIAKSNTPPMYLQADIEAFDIRELTPKFDVILLEPPLEEYYRETGITANEKCWTWDDIMKLEIDEIAAPRSFIFLWCGSGEGLDLGRVCLRKWGYRRCEDICWIKTNKNNPGKTKTLDPKAVFQRTKEHCLMGIKGTVKRSTDGDFIHANVDIDLIITEEPEIGNIEKPVEIFHIIEHFCLGRRRLHLFGRDSTIRPGWLTVGPTLTNSNYNAETYASYFSAPNSYLTGCTEEIERLRPKSPPPKSKSDRGGGAPRGGGRGGTSAGRGRERNRSNFRGERGGFRGGRGGAHRGGFPPR
uniprot
proteins
function
FUNCTION: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27281194, PubMed:27373337, PubMed:29348140). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27281194, PubMed:27373337, PubMed:27627798, PubMed:29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity). {ECO:0000250|UniProtKB:Q3UIK4, ECO:0000269|PubMed:24316715, ECO:0000269|PubMed:24407421, ECO:0000269|PubMed:25719671, ECO:0000269|PubMed:27281194, ECO:0000269|PubMed:27373337, ECO:0000269|PubMed:27627798, ECO:0000269|PubMed:29348140}.
uniprot
proteins
mass_dalton
52150
uniprot
proteins
mutagenesis
MUTAGEN 63..65; /note="KRK->GGG: Does not affect nuclear localization."; /evidence="ECO:0000269|PubMed:29348140"; MUTAGEN 173; /note="D->A: Little or no effect on S-adenosyl-L-methionine-binding or methyltransferase activity; when associated with A-192."; /evidence="ECO:0000269|PubMed:27281194"; MUTAGEN 192; /note="E->A: Little or no effect on methyltransferase activity. Little or no effect on S-adenosyl-L-methionine-binding or methyltransferase activity; when associated with A-173."; /evidence="ECO:0000269|PubMed:27281194, ECO:0000269|PubMed:27373337, ECO:0000269|PubMed:27627798"; MUTAGEN 198; /note="Y->A: Does not affect methyltransferase activity of the heterodimer complex formed with METTL3."; /evidence="ECO:0000269|PubMed:27627798"; MUTAGEN 245; /note="R->E: Reduced RNA-binding. Reduced RNA-binding; when associated with E-255."; /evidence="ECO:0000269|PubMed:27281194"; MUTAGEN 254..255; /note="RR->AA: Strongly reduced methyltransferase activity of the heterodimer complex formed with METTL3."; /evidence="ECO:0000269|PubMed:27627798"; MUTAGEN 255; /note="R->E: Reduced RNA-binding; when associated with E-245."; /evidence="ECO:0000269|PubMed:27281194"; MUTAGEN 297..298; /note="KR->EE: Reduced RNA-binding."; /evidence="ECO:0000269|PubMed:27281194"; MUTAGEN 298; /note="R->P: Strongly decreased methyltransferase activity of the heterodimer complex formed with METTL3, probably due to reduced RNA-binding."; /evidence="ECO:0000269|PubMed:27373337"; MUTAGEN 312; /note="D->A: Decreased methyltransferase activity of the heterodimer complex formed with METTL3."; /evidence="ECO:0000269|PubMed:27373337"; MUTAGEN 338; /note="C->A: Does not affect methyltransferase activity of the heterodimer complex formed with METTL3."; /evidence="ECO:0000269|PubMed:27627798"; MUTAGEN 362..363; /note="PT->AA: Little or no effect on methyltransferase activity of the heterodimer complex formed with METTL3."; /evidence="ECO:0000269|PubMed:27627798"; MUTAGEN 399; /note="S->A,E: Does not affect interaction with METTL3."; /evidence="ECO:0000269|PubMed:29348140
uniprot
proteins
protein_family
MT-A70-like family
uniprot
proteins
pubmed
10997877
uniprot
proteins
pubmed
12168954
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15815621
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
24316715
uniprot
proteins
pubmed
24407421
uniprot
proteins
pubmed
24981863
uniprot
proteins
pubmed
25719671
uniprot
proteins
pubmed
26458103
uniprot
proteins
pubmed
27281194
uniprot
proteins
pubmed
27373337
uniprot
proteins
pubmed
27627798
uniprot
proteins
pubmed
29348140
uniprot
proteins
pubmed
29507755
uniprot
proteins
sequence_length
456
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24316715, ECO:0000269|PubMed:24407421, ECO:0000269|PubMed:26458103, ECO:0000269|PubMed:29348140}.
uniprot
proteins