Ifitm3
entrez
66141
canonical
uniprot
Q9CQW9
canonical
ensembl
ENSMUSG00000025492
resolver
ensp
ENSMUSP00000026565
resolver
enst
ENSMUST00000026565
resolver
genesymbol
Ifitm3
resolver
uniprot
Q9D8L6
resolver
uniprot_entry
IFM3_MOUSE
resolver
uniprot_sec
Q9D8L6
resolver
amino_acid_sequence
MNHTSQAFITAASGGQPPNYERIKEEYEVAEMGAPHGSASVRTTVINMPREVSVPDHVVWSLFNTLFMNFCCLGFIAYAYSVKSRDRKMVGDVTGAQAYASTAKCLNISTLVLSILMVVITIVSVIIIVLNAQNLHT
uniprot
proteins
function
FUNCTION: IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV) and vesicular stomatitis virus (VSV) (PubMed:33270927). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV and SARS-CoV-2 S protein-mediated viral entry and VSV G protein-mediated viral entry (PubMed:33270927). Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state. In hepatocytes, IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation. IFITM2 and IFITM3 display anti-HCV activity that may complement the anti-HCV activity of IFITM1 by inhibiting the late stages of HCV entry, possibly in a coordinated manner by trapping the virion in the endosomal pathway and targeting it for degradation at the lysosome. Exerts opposing activities on SARS-CoV-2, including amphipathicity-dependent restriction of virus at endosomes and amphipathicity-independent enhancement of infection at the plasma membrane. {ECO:0000269|PubMed:12124616, ECO:0000269|PubMed:18505827, ECO:0000269|PubMed:21253575, ECO:0000269|PubMed:22467717, ECO:0000269|PubMed:33270927}.
uniprot
proteins
mass_dalton
14954
uniprot
proteins
mutagenesis
MUTAGEN 20; /note="Y->A: Accumulates at the plasma membrane. No effect on anti-SARS-CoV-2 activity."; /evidence="ECO:0000269|PubMed:33270927"; MUTAGEN 71..72; /note="CC->AA: No effect on SARS-CoV-2 infection; when associated with A-105."; /evidence="ECO:0000269|PubMed:33270927"; MUTAGEN 105; /note="C->A: No effect on SARS-CoV-2 infection; when associated with 71-A-A-72."; /evidence="ECO:0000269|PubMed:33270927
uniprot
proteins
post_translational_modification
PTM: Polyubiquitinated with both 'Lys-48' and 'Lys-63' linkages. Ubiquitination negatively regulates antiviral activity. Lys-24 is the most prevalent ubiquitination site. {ECO:0000250|UniProtKB:Q01628}.; PTM: Phosphorylation at Tyr-20 is required for endosomal and lysosomal location. {ECO:0000250|UniProtKB:Q01628}.
uniprot
proteins
protein_family
CD225/Dispanin family
uniprot
proteins
pubmed
12124616
uniprot
proteins
pubmed
12659663
uniprot
proteins
pubmed
14516695
uniprot
proteins
pubmed
15184081
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16326387
uniprot
proteins
pubmed
16395393
uniprot
proteins
pubmed
18505827
uniprot
proteins
pubmed
19144319
uniprot
proteins
pubmed
21166591
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21253575
uniprot
proteins
pubmed
22363774
uniprot
proteins
pubmed
22446628
uniprot
proteins
pubmed
22467717
uniprot
proteins
pubmed
33270927
uniprot
proteins
sequence_length
137
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q01628}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q01628}. Late endosome membrane {ECO:0000269|PubMed:33270927}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q01628}. Early endosome membrane {ECO:0000269|PubMed:33270927}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q01628}. Lysosome membrane {ECO:0000269|PubMed:33270927}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q01628}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q01628}. Note=Co-localizes with BRI3 isoform 1 at the perinuclear region. {ECO:0000250|UniProtKB:Q01628}.
uniprot
proteins
transmembrane_region
TRANSMEM 110..130; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins