Dgcr8
entrez
94223
canonical
uniprot
Q9EQM6
canonical
ensembl
ENSMUSG00000022718
resolver
ensp
ENSMUSP00000009321
resolver
ensp
ENSMUSP00000253442
resolver
ensp
ENSMUSP00000253454
resolver
enst
ENSMUST00000009321
resolver
genesymbol
Dgcr8
resolver
uniprot_entry
DGCR8_MOUSE
resolver
amino_acid_sequence
METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGVERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGALGQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCTVDV
uniprot
proteins
function
FUNCTION: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis (PubMed:17259983). Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (By similarity). Involved in the silencing of embryonic stem cell self-renewal (PubMed:17259983). Also plays a role in DNA repair by promoting the recruitment of RNF168 to RNF8 and MDC1 at DNA double-strand breaks and subsequently the clearance of DNA breaks (By similarity). {ECO:0000250|UniProtKB:Q8WYQ5, ECO:0000269|PubMed:17259983}.
uniprot
proteins
mass_dalton
86321
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Ser-677 by ATM upon radiation, which is crucial for its stability. {ECO:0000250|UniProtKB:Q8WYQ5}.; PTM: Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization. {ECO:0000250|UniProtKB:Q8WYQ5}.
uniprot
proteins
pubmed
12705904
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
17259983
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
26255770
uniprot
proteins
sequence_length
773
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8WYQ5}. Nucleus, nucleolus {ECO:0000250|UniProtKB:Q8WYQ5}. Note=Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies. {ECO:0000250|UniProtKB:Q8WYQ5}.
uniprot
proteins