NCBP2
entrez
22916
canonical
uniprot
P52298
canonical
ensembl
ENSG00000114503
resolver
ensp
ENSP00000326806
resolver
ensp
ENSP00000394105
resolver
ensp
ENSP00000397619
resolver
ensp
ENSP00000412785
resolver
ensp
ENSP00000413518
resolver
enst
ENST00000422610
resolver
enst
ENST00000447325
resolver
genesymbol
NCBP2
resolver
genesymbol_syn
CBP20
resolver
hgnc
HGNC:7659
resolver
uniprot
A0A9K3Y7M6
resolver
uniprot
A1QEE0
resolver
uniprot
B2RE91
resolver
uniprot
B4DMK7
resolver
uniprot
D3DXB7
resolver
uniprot
E9PAR5
resolver
uniprot
F6WVI0
resolver
uniprot
Q14924
resolver
uniprot
Q2TS50
resolver
uniprot_entry
A1QEE0_HUMAN
resolver
uniprot_entry
F6WVI0_HUMAN
resolver
uniprot_entry
NCBP2_HUMAN
resolver
uniprot_sec
A0A9K3Y7M6
resolver
uniprot_sec
B2RE91
resolver
uniprot_sec
B4DMK7
resolver
uniprot_sec
D3DXB7
resolver
uniprot_sec
E9PAR5
resolver
uniprot_sec
Q14924
resolver
uniprot_sec
Q2TS50
resolver
amino_acid_sequence
MSGGLLKALRSDSYVELSQYRDQHFRGDNEEQEKLLKKSCTLYVGNLSFYTTEEQIYELFSKSGDIKKIIMGLDKMKKTACGFCFVEYYSRADAENAMRYINGTRLDDRIIRTDWDAGFKEGRQYGRGRSGGQVRDEYRQDYDAGRGGYGKLAQNQ
uniprot
proteins
function
FUNCTION: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (PubMed:26382858). {ECO:0000269|PubMed:11551508, ECO:0000269|PubMed:15361857, ECO:0000269|PubMed:17190602, ECO:0000269|PubMed:17363367, ECO:0000269|PubMed:17873884, ECO:0000269|PubMed:18369367, ECO:0000269|PubMed:19632182, ECO:0000269|PubMed:26382858}.
uniprot
proteins
mass_dalton
18001
uniprot
proteins
mutagenesis
MUTAGEN 20; /note="Y->A: Abolishes mRNA cap-binding."; /evidence="ECO:0000269|PubMed:12374755"; MUTAGEN 20; /note="Y->F: Strongly impairs mRNA cap-binding."; /evidence="ECO:0000269|PubMed:12374755"; MUTAGEN 25; /note="F->A: Does not affect mRNA cap-binding."; /evidence="ECO:0000269|PubMed:11545740"; MUTAGEN 43; /note="Y->A: Abolishes mRNA cap-binding."; /evidence="ECO:0000269|PubMed:11545740, ECO:0000269|PubMed:12374755"; MUTAGEN 43; /note="Y->F: Does not affect mRNA cap-binding."; /evidence="ECO:0000269|PubMed:11545740, ECO:0000269|PubMed:12374755"; MUTAGEN 46; /note="N->A: Does not affect mRNA cap-binding."; /evidence="ECO:0000269|PubMed:11545740"; MUTAGEN 83; /note="F->A: Abolishes mRNA cap-binding."; /evidence="ECO:0000269|PubMed:11545740"; MUTAGEN 85; /note="F->A: Impairs mRNA cap-binding."; MUTAGEN 112; /note="R->A,T: Does not affect mRNA cap-binding."; /evidence="ECO:0000269|PubMed:12374755"; MUTAGEN 114; /note="D->A: Does not affect mRNA cap-binding."; /evidence="ECO:0000269|PubMed:11545740"; MUTAGEN 116; /note="D->A: Abolishes mRNA cap-binding."; /evidence="ECO:0000269|PubMed:11545740"; MUTAGEN 119; /note="F->A: Does not affect mRNA cap-binding."; /evidence="ECO:0000269|PubMed:11545740"; MUTAGEN 138; /note="Y->A: Does not affect mRNA cap-binding."; /evidence="ECO:0000269|PubMed:12374755
uniprot
proteins
protein_family
RRM NCBP2 family
uniprot
proteins
pubmed
11545740
uniprot
proteins
pubmed
11551508
uniprot
proteins
pubmed
12374755
uniprot
proteins
pubmed
12434151
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15361857
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16641997
uniprot
proteins
pubmed
17190602
uniprot
proteins
pubmed
17363367
uniprot
proteins
pubmed
17525332
uniprot
proteins
pubmed
17873884
uniprot
proteins
pubmed
18369367
uniprot
proteins
pubmed
19632182
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24129315
uniprot
proteins
pubmed
26382858
uniprot
proteins
pubmed
34564892
uniprot
proteins
pubmed
7478990
uniprot
proteins
pubmed
7651522
uniprot
proteins
pubmed
8601613
uniprot
proteins
pubmed
9111328
uniprot
proteins
sequence_length
156
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:8601613}. Cytoplasm {ECO:0000269|PubMed:8601613}.
uniprot
proteins