Adat3
entrez
100113398
canonical
uniprot
Q6PAT0
canonical
ensembl
ENSMUSG00000035370
resolver
ensembl
ENSMUSG00000113640
resolver
ensp
ENSMUSP00000040551
resolver
ensp
ENSMUSP00000136259
resolver
ensp
ENSMUSP00000151394
resolver
enst
ENSMUST00000038411
resolver
enst
ENSMUST00000178231
resolver
enst
ENSMUST00000218067
resolver
genesymbol
Adat3
resolver
uniprot_entry
ADAT3_MOUSE
resolver
amino_acid_sequence
MEPTSGFAEQPGPVKAESEEQEPAQWQALPVLSEQQSGAVELILAYAAPVLDKRQTSRLLREVSAVYPLPAQPHLKRVRPSRSAGGAQSSDLLLCLAGPSAGPRSLAELLPRPAVDPRGLGTPFLVPVPARPPLTRSQFEEARAHWPTSFHEDKQVTSALAGQLFSTQERAAMQTHMERAVCAAQRAAAQGLRAVGAVVVDPASDRVLATGHDCSSVASPLLHAVMVCIDLVAQGQGRGSCDLRSHPACSFTQATATQGARAGSVRKLDEDSLPYVCTGYDLYVTREPCVMCAMALVHARIQRVFYGAPSPDGALGTLFRVHARPDLNHRFQVFRGILEDQCRQLDPDP
uniprot
proteins
function
FUNCTION: Non-catalytic subunit of the tRNA-specific adenosine-34 deaminase complex, composed of the ADAT2 catalytic subunit and the ADAT3 regulatory subunit, which deaminates adenosine-34 (the first, also called wobble position of the anticodon) to inosine in many tRNAs (PubMed:34057470). Inosine-34 allows the decoding of 3 different nucleotides at the third position of mRNA codons, as inosine is able to pair with U, C, and A (PubMed:34057470). Required for binding of the ADAT2-ADAT3 complex to tRNA through its N-terminus, which rotates with respect to the catalytic domain of the complex, formed by ADAT2 and the ADAT3 C-terminal domain, to position the tRNA anticodon stem-loop correctly in the ADAT2 active site (PubMed:34057470). The ADAT2-ADAT3 complex is required for radial migration of projection neurons in the developing brain cortex, and the catalytic activity of the complex is necessary for this function (PubMed:40120092). {ECO:0000269|PubMed:34057470, ECO:0000269|PubMed:40120092}.
uniprot
proteins
mass_dalton
37517
uniprot
proteins
mutagenesis
MUTAGEN 128; /note="V->I,L: Does not affect tRNA-binding activity. Reduces neuronal migration in the embryonic cortex."; /evidence="ECO:0000269|PubMed:34057470"; MUTAGEN 128; /note="V->M: Does not affect tRNA binding or solubility but the ADAT2-ADAT3 heterodimer displays reduced deamination activity. Perturbs but does not prevent folding of the ADAT3 N-terminal domain. Reduces neuronal migration in the embryonic cortex. Reduces protein solubility which is restored following coexpression with wild-type ADAT2."; /evidence="ECO:0000269|PubMed:34057470, ECO:0000269|PubMed:40120092"; MUTAGEN 180; /note="A->L: Severe decrease in deaminase activity of the ADAT2-ADAT3 complex."; /evidence="ECO:0000269|PubMed:40120092"; MUTAGEN 180; /note="A->V: No effect on deaminase activity of the ADAT2-ADAT3 complex."; /evidence="ECO:0000269|PubMed:40120092"; MUTAGEN 225; /note="V->E: Loss of ADAT2-ADAT3 complex deaminase activity; when associated with 'A-73' of ADAT2."; /evidence="ECO:0000269|PubMed:34057470
uniprot
proteins
protein_family
ADAT3 subfamily
uniprot
proteins
protein_family
Cytidine and deoxycytidylate deaminase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
34057470
uniprot
proteins
pubmed
40120092
uniprot
proteins
sequence_length
349
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:40120092}. Cytoplasm {ECO:0000269|PubMed:40120092}. Note=The ADAT2-ADAT3 complex shows a diffuse expression pattern in both the cytoplasm and the nucleus. {ECO:0000269|PubMed:40120092}.
uniprot
proteins