Dph3
entrez
105638
canonical
uniprot
Q8K0W9
canonical
ensembl
ENSMUSG00000021905
resolver
ensp
ENSMUSP00000022461
resolver
ensp
ENSMUSP00000068491
resolver
ensp
ENSMUSP00000120583
resolver
enst
ENSMUST00000022461
resolver
enst
ENSMUST00000067955
resolver
enst
ENSMUST00000124303
resolver
genesymbol
Dph3
resolver
genesymbol_syn
Desr1
resolver
genesymbol_syn
Zcsl2
resolver
uniprot
D3YXV3
resolver
uniprot_entry
D3YXV3_MOUSE
resolver
uniprot_entry
DPH3_MOUSE
resolver
amino_acid_sequence
MAVFHDEVEIEDFQYDEDSETYFYPCPCGDNFAITKEDLENGEDVATCPSCSLIIKVIYDKDQFMCGETVPAPSTNKELVKC
uniprot
proteins
function
FUNCTION: Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Acts as an electron donor to reduce the Fe-S cluster in DPH1-DPH2 keeping the [4Fe-4S] clusters in the active and reduced state. Restores iron to DPH1-DPH2 iron-sulfur clusters which have degraded from [4Fe-4S] to [3Fe-4S] by donating an iron atom to reform [4Fe-4S] clusters, in a manner dependent on the presence of elongation factor 2 and SAM. Associates with the elongator complex and is required for tRNA Wobble base modifications mediated by the elongator complex. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). {ECO:0000250|UniProtKB:Q3E840}.
uniprot
proteins
mass_dalton
9286
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis. {ECO:0000305}.
uniprot
proteins
protein_family
DPH3 family
uniprot
proteins
pubmed
14527407
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16648478
uniprot
proteins
pubmed
23185508
uniprot
proteins
sequence_length
82
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96FX2}. Nucleus {ECO:0000250|UniProtKB:Q96FX2}.
uniprot
proteins