Pias4
entrez
59004
canonical
uniprot
Q9JM05
canonical
ensembl
ENSMUSG00000004934
resolver
ensp
ENSMUSP00000005064
resolver
enst
ENSMUST00000005064
resolver
genesymbol
Pias4
resolver
genesymbol_syn
Piasg
resolver
uniprot
Q3ULQ6
resolver
uniprot
Q8R165
resolver
uniprot_entry
PIAS4_MOUSE
resolver
uniprot_entry
Q3ULQ6_MOUSE
resolver
uniprot_sec
Q8R165
resolver
amino_acid_sequence
MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKSAEPGPQAPRPLDPLALHSMPRTPLSGPTVDYPVLYGKYLNGLGRLPTKTLKPEVRLVKLPFFNMLDELLKPTELVPQSAEKLQESPCIFALTPRQVEMIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSDLLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAAYDQLIIDGLLSKILSECEGADEIEFLAEGSWRPIRAEKEPSCSPQGPILVLGTSDANGLAPASSTPGIGSGLSGPGSAGSGAGAAGSLENGKTGADVVDLTLDSSSSSEDEDEDEDDDEDEDEGPRPKRRCPFQKGLVPAC
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
function
FUNCTION: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (By similarity). Mediates sumoylation of ALKBH5, AXIN1, CEBPA, KLF8, GATA2, PARK7, HERC2, MYB, TCF4 and RNF168 (By similarity). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway (By similarity). Involved in gene silencing (By similarity). In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (By similarity). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (PubMed:11731474). Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR) (By similarity). Mediates sumoylation of PARP1 in response to PARP1 trapping to chromatin (By similarity). Mediates sumoylation of KLF8, repressing KLF8 transcriptional activity and cell cycle progression into G(1) phase (By similarity). Sumoylates ALKBH5 downstream of MAPK8/JNK1 and MAPK9/JNK2 in response to reactive oxygen species (ROS), inhibiting ALKBH5 RNA demethylase activity (By similarity). {ECO:0000250|UniProtKB:Q8N2W9, ECO:0000269|PubMed:11731474}.
uniprot
proteins
mass_dalton
55570
uniprot
proteins
mutagenesis
MUTAGEN 330; /note="C->S: Abrogates sumoylation of LEF1 and increases LEF1-mediated transcriptional activity; when associated with S-335; A-337 and S-340."; /evidence="ECO:0000269|PubMed:11731474"; MUTAGEN 335; /note="C->S: Abrogates sumoylation of LEF1 and increases LEF1-mediated transcriptional activity; when associated with S-330; A-337 and S-340."; /evidence="ECO:0000269|PubMed:11731474"; MUTAGEN 337; /note="H->A: Abrogates sumoylation of LEF1 and increases LEF1-mediated transcriptional activity; when associated with S-330; S-335 and S-340."; /evidence="ECO:0000269|PubMed:11731474"; MUTAGEN 340; /note="C->S: Abrogates sumoylation of LEF1 and increases LEF1-mediated transcriptional activity; when associated with S-330; S-335 and A-337."; /evidence="ECO:0000269|PubMed:11731474"; MUTAGEN 470..474; /note="SSSSS->AAAAA: No effect on sumoylation of LEF1, nor on LEF1-binding."; /evidence="ECO:0000269|PubMed:11731474
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein sumoylation. {ECO:0000250|UniProtKB:Q8N2W9}.
uniprot
proteins
post_translational_modification
PTM: Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate. {ECO:0000269|PubMed:11731474}.; PTM: Ubiquitinated by TRIM32 upon treatment with UVB and TNF. {ECO:0000269|PubMed:16816390}.
uniprot
proteins
protein_family
PIAS family
uniprot
proteins
pubmed
10854042
uniprot
proteins
pubmed
11731474
uniprot
proteins
pubmed
12750312
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16352559
uniprot
proteins
pubmed
16816390
uniprot
proteins
pubmed
21183079
uniprot
proteins
sequence_length
507
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus, PML body {ECO:0000269|PubMed:11731474, ECO:0000269|PubMed:16816390}. Note=Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies. Accumulates in the cytoplasm upon treatment with UVB and TNF. {ECO:0000250|UniProtKB:Q8N2W9}.
uniprot
proteins