Rnf145
entrez
74315
canonical
uniprot
Q5SWK7
canonical
ensembl
ENSMUSG00000019189
resolver
ensp
ENSMUSP00000019333
resolver
ensp
ENSMUSP00000098881
resolver
ensp
ENSMUSP00000296909
resolver
ensp
ENSMUSP00000296910
resolver
ensp
ENSMUSP00000296931
resolver
enst
ENSMUST00000019333
resolver
enst
ENSMUST00000101327
resolver
genesymbol
Rnf145
resolver
uniprot
F6VL67
resolver
uniprot
Q3UTM8
resolver
uniprot
Q8BU61
resolver
uniprot
Q8BXX5
resolver
uniprot
Q9CXG1
resolver
uniprot_entry
F6VL67_MOUSE
resolver
uniprot_entry
Q3UTM8_MOUSE
resolver
uniprot_entry
Q8BU61_MOUSE
resolver
uniprot_entry
RN145_MOUSE
resolver
uniprot_sec
Q8BXX5
resolver
uniprot_sec
Q9CXG1
resolver
amino_acid_sequence
MAAKEKLEAVLNVALRVPSIMLLDVLYRWDVSSFFQQIQRSSLNNNPLFQYKYLALNMHYVGYILSVVLLTLPRQHLVQLYLYFVTALLLYAGHQISRDYVRSELESAYEGPMYLEPLSMNRFTTALIGQLVVCTLCSCVMKTKQIWLFSAHMLPLLARLCLVPLETIVIINKFAMIFTGLEVLYFLGSNLLVPYNLAKSAYRELVQVVEVYGLLALGMSLWNQLVVPVLFMVFWLVLFALQIYSYFSTRDQPASRERLLFLFLTSIAECCSTPYSLLGLVFTVSFVALGVLTLCKFYLQGYRAFMNDPAMNRGMTEGVTLLILAVQTGLIELQVVHRAFLLSIILFIVVASILQSMLEIADPIVLALGASRDKSLWKHFRAVSLCLFLLVFPAYMAYMICQFFHMDFWLLIIISSSILTSLQVLGTLFIYVLFMVEEFRKEPVENMDDVIYYVNGTYRLLEFVVALCVVAYGVSETIFGEWTVMGSMIIFIHSYYNVWLRAQLGWKSFLLRRDAVNKIKSLPVATQEQLEKHNDICAICYQDMKSAVITPCSHFFHAGCLKKWLYVQDTCPLCHCHLKNSSQLPGLGTEAAPQPPAGAEQNIVLQEGPEPPDHESPPGTGTQEGSGDSSEHINRGSASQEGAADAGEGPQIPEGEVCPVESA
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
function
FUNCTION: E3 ubiquitin ligase that catalyzes the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. In response to bacterial infection, negatively regulates the phagocyte oxidative burst by controlling the turnover of the NADPH oxidase complex subunits. Promotes monoubiquitination of CYBA and 'Lys-48'-linked polyubiquitination and degradation of CYBB NADPH oxidase catalytic subunits, both essential for the generation of antimicrobial reactive oxygen species (PubMed:26194095). Involved in the maintenance of cholesterol homeostasis. In response to high sterol concentrations ubiquitinates HMGCR, a rate-limiting enzyme in cholesterol biosynthesis, and targets it for degradation. The interaction with INSIG1 is required for this function (PubMed:29374057). In addition, triggers ubiquitination of SCAP, likely inhibiting its transport to the Golgi apparatus and the subsequent processing/maturation of SREBPF2, ultimately down-regulating cholesterol biosynthesis (PubMed:29068315). {ECO:0000269|PubMed:26194095, ECO:0000269|PubMed:29068315, ECO:0000269|PubMed:29374057}.
uniprot
proteins
mass_dalton
74648
uniprot
proteins
mutagenesis
MUTAGEN 81..84; /note="YLYF->AAAA: Impairs interaction with INSIG1."; /evidence="ECO:0000269|PubMed:29374057"; MUTAGEN 537; /note="C->A: Abolishes E3 ubiquitin ligase activity. Impairs ubiquitination of SCAP and functional regulation of cholesterol biosynthesis. Impairs sterol-induced ubiquitination and degradation of HMGCR."; /evidence="ECO:0000269|PubMed:29068315, ECO:0000269|PubMed:29374057
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
26194095
uniprot
proteins
pubmed
29068315
uniprot
proteins
pubmed
29374057
uniprot
proteins
sequence_length
663
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:26194095, ECO:0000269|PubMed:29068315, ECO:0000269|PubMed:29374057}; Multi-pass membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 53..73; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 77..97; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 123..143; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 146..166; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 168..188; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 205..222; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 225..245; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 275..295; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 316..336; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 340..360; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 384..404; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 410..430; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 460..480; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 482..502; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins