POLN
entrez
353497
canonical
uniprot
Q7Z5Q5
canonical
ensembl
ENSG00000130997
resolver
ensp
ENSP00000435506
resolver
genesymbol
POLN
resolver
hgnc
HGNC:18870
resolver
uniprot
A2A336
resolver
uniprot
B4E158
resolver
uniprot
Q4TTW4
resolver
uniprot
Q6ZNF4
resolver
uniprot_entry
DPOLN_HUMAN
resolver
uniprot_sec
A2A336
resolver
uniprot_sec
B4E158
resolver
uniprot_sec
Q4TTW4
resolver
uniprot_sec
Q6ZNF4
resolver
activity_regulation
ACTIVITY REGULATION: Inhibited by ddTTP. {ECO:0000269|PubMed:16787914}.
uniprot
proteins
amino_acid_sequence
MENYEALVGFDLCNTPLSSVAQKIMSAMHSGDLVDSKTWGKSTETMEVINKSSVKYSVQLEDRKTQSPEKKDLKSLRSQTSRGSAKLSPQSFSVRLTDQLSADQKQKSISSLTLSSCLIPQYNQEASVLQKKGHKRKHFLMENINNENKGSINLKRKHITYNNLSEKTSKQMALEEDTDDAEGYLNSGNSGALKKHFCDIRHLDDWAKSQLIEMLKQAAALVITVMYTDGSTQLGADQTPVSSVRGIVVLVKRQAEGGHGCPDAPACGPVLEGFVSDDPCIYIQIEHSAIWDQEQEAHQQFARNVLFQTMKCKCPVICFNAKDFVRIVLQFFGNDGSWKHVADFIGLDPRIAAWLIDPSDATPSFEDLVEKYCEKSITVKVNSTYGNSSRNIVNQNVRENLKTLYRLTMDLCSKLKDYGLWQLFRTLELPLIPILAVMESHAIQVNKEEMEKTSALLGARLKELEQEAHFVAGERFLITSNNQLREILFGKLKLHLLSQRNSLPRTGLQKYPSTSEAVLNALRDLHPLPKIILEYRQVHKIKSTFVDGLLACMKKGSISSTWNQTGTVTGRLSAKHPNIQGISKHPIQITTPKNFKGKEDKILTISPRAMFVSSKGHTFLAADFSQIELRILTHLSGDPELLKLFQESERDDVFSTLTSQWKDVPVEQVTHADREQTKKVVYAVVYGAGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAAIAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIPECAALVRRTMESLEQVQALELQLQVPLKVSLSAGRSWGHLVPLQEAWGPPPGPCRTESPSNSLAAPGSPASTQPPPLHFSPSFCL
uniprot
proteins
ec_number
2.7.7.7
uniprot
proteins
function
FUNCTION: DNA polymerase with very low fidelity that catalyzes considerable misincorporation by inserting dTTP opposite a G template, and dGTP opposite a T template (PubMed:16787914, PubMed:17118716). Is the least accurate of the DNA polymerase A family (i.e. POLG, POLN and POLQ) (PubMed:17118716). Can perform accurate translesion DNA synthesis (TLS) past a 5S-thymine glycol. Can perform efficient strand displacement past a nick or a gap and gives rise to an amount of product similar to that on non-damaged template. Has no exonuclease activity (PubMed:16787914). Error-prone DNA polymerase that preferentially misincorporates dT regardless of template sequence (PubMed:25775266). May play a role in TLS during interstrand cross-link (ICL) repair (PubMed:19908865). May be involved in TLS when genomic replication is blocked by extremely large major groove DNA lesions. May function in the bypass of some DNA-protein and DNA-DNA cross-links. May have a role in cellular tolerance to DNA cross-linking agents (PubMed:20102227). Involved in the repair of DNA cross-links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with HELQ helicase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links (PubMed:19995904). {ECO:0000269|PubMed:16787914, ECO:0000269|PubMed:17118716, ECO:0000269|PubMed:19908865, ECO:0000269|PubMed:19995904, ECO:0000269|PubMed:20102227, ECO:0000269|PubMed:25775266}.
uniprot
proteins
mass_dalton
100307
uniprot
proteins
mutagenesis
MUTAGEN 623; /note="D->A: Abolishes catalytic activity."; /evidence="ECO:0000269|PubMed:12794064, ECO:0000269|PubMed:16787914"; MUTAGEN 675; /note="E->R: Reduces polymerase activity. No effect on accuracy."; /evidence="ECO:0000269|PubMed:25775266"; MUTAGEN 679; /note="K->A: No effect on polymerase activity. Increases accuracy by ten-fold."; /evidence="ECO:0000269|PubMed:25775266
uniprot
proteins
protein_family
DNA polymerase type-A family
uniprot
proteins
pubmed
12794064
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15815621
uniprot
proteins
pubmed
16787914
uniprot
proteins
pubmed
17118716
uniprot
proteins
pubmed
19908865
uniprot
proteins
pubmed
19995904
uniprot
proteins
pubmed
20102227
uniprot
proteins
pubmed
25775266
uniprot
proteins
sequence_length
900
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12794064}.
uniprot
proteins