Bub1b
entrez
12236
canonical
uniprot
Q9Z1S0
canonical
ensembl
ENSMUSG00000040084
resolver
ensp
ENSMUSP00000037126
resolver
ensp
ENSMUSP00000185712
resolver
enst
ENSMUST00000038341
resolver
genesymbol
Bub1b
resolver
genesymbol_syn
Mad3l
resolver
uniprot
A2ARS1
resolver
uniprot
Q3UZD6
resolver
uniprot_entry
BUB1B_MOUSE
resolver
uniprot_entry
Q3UZD6_MOUSE
resolver
uniprot_sec
A2ARS1
resolver
activity_regulation
ACTIVITY REGULATION: Kinase activity stimulated by CENPE. {ECO:0000269|PubMed:12925705}.
uniprot
proteins
amino_acid_sequence
MAEASEAMCLEGAEWELSKENIQPLRHGRVMSTLQGALAKQESAGHTALQQQKRAFESEIRFYSGDDPLDVWDRYINWTEQNYPQGGKESNMSALVERAIEALQGETRYYNDPRFLSLWIKLGHLCNEPLDMYSYLQSQGIGVSLAQFYISWAEEYEARENFKKADIIFQEGIERKAEPLDRLQSQHRQFQSRVSRQAFLALGNEEEEALEPSEPQRSSLAELKSRGKKMARAPISRVGGALKAPGQSRGFLNAVPQPVHGNRRITVFDENADTASRTELSKPVAQPWMAPPVPRAKENELQPGPWSTDRPAGRRPHDNPASVTSIPSVLPSFTPYVEESAQQTVMTPCKIEPSINHVLSTRKPGREEGDPLQRVQSHQQGCEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAKKREEMQKQIEEMERRLKAMQAVQQEGAGGQQEEKMPTEDPARLQIASGPQEMSGVPLSCSICPLSSNPREISPAENILQEQPDSKGSSMPFSIFDESLSDKKDKSPATGGPQVLNAQRRPLSVLKTTEVGTTNEDVSPDICDELTELEPLSEDAIITGFRNVTLCPNPEDTCDFARAARLASTPFHEILSSKGIAADPEGLLQEEDLDGKAAEAHHTVHHQALIIKKLSPIIEESREATHSSGFSRSSSSAPSTSSIKGFQLLEKLELTNDGAEDAIQSPWCSQYRLQLLKSLLELSAFAEFSVEDRPMPVLEIGKEIELGPEDYVIKQEHLTCDDYRLFWVAPRSSAELTMIKASSQPIPWDFYINLKLKERLNEDYDQLCSCCQYQDGHVVWYQYINCSTLQNLLQHSEFVTHEIIVLIIYNLLTIVEKLHRAEIVHGDLSPRSLILRNRIHDPYDYVNKDDHAVRIMDFSYSVDLRVQLDAFAYSGFRTAQILEGQKILANCSSPYHVDLLGIADLAHLLLFKEHLHVFWDGLLWKLSQSTSELKDSELWNKFFVRILNASDKSTVSVLGELAAEMGGAFDATFHSHLNRALWKLGKTISPEALLTQQDKQPGGSQSPA
uniprot
proteins
disease_involvement
DISEASE: Note=Defects in Bub1b are involved in the development of lung and intestinal adenocarcinomas after exposure to a carcinogen. {ECO:0000269|PubMed:14744753}.
uniprot
proteins
ec_number
2.7.11.1
uniprot
proteins
function
FUNCTION: Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle (By similarity). The MCC, which consists of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3, is a key player of the spindle assembly checkpoint (By similarity). The MCC-bound APC/C complex is inactive and this delays the metaphase/anaphase transition (By similarity). When the spindle assembly checkpoint is silenced, the MCC-APC/C complex recruits the E2 enzyme UBCH10 and triggers the auto-ubiquitination of CDC20 in the MCC, thereby reactivating APC/C for transition into anaphase (By similarity). MCC also monitors kinetochore activities that depend on the kinetochore motor CENPE (By similarity). Required for kinetochore localization of CENPE (By similarity). Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification (By similarity). May play a role for tumor suppression (PubMed:14744753). {ECO:0000250|UniProtKB:O60566, ECO:0000269|PubMed:14744753}.
uniprot
proteins
mass_dalton
118392
uniprot
proteins
post_translational_modification
PTM: Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. {ECO:0000250|UniProtKB:O60566}.; PTM: Acetylation at Lys-243 regulates its degradation and timing in anaphase entry. {ECO:0000250|UniProtKB:O60566}.; PTM: Ubiquitinated. Degraded by the proteasome. Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex. {ECO:0000250|UniProtKB:O60566}.; PTM: Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore (By similarity). {ECO:0000250|UniProtKB:O60566}.; PTM: Autophosphorylated in vitro. Intramolecular autophosphorylation stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-659 and Ser-1033 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase. {ECO:0000250|UniProtKB:O60566}.; PTM: Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-603. {ECO:0000250|UniProtKB:O60566}.
uniprot
proteins
protein_family
BUB1 subfamily
uniprot
proteins
protein_family
Protein kinase superfamily
uniprot
proteins
protein_family
Ser/Thr protein kinase family
uniprot
proteins
pubmed
12925705
uniprot
proteins
pubmed
14744753
uniprot
proteins
pubmed
15208629
uniprot
proteins
pubmed
17925231
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
9889005
uniprot
proteins
sequence_length
1052
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O60566}. Nucleus {ECO:0000250|UniProtKB:O60566}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:12925705, ECO:0000269|PubMed:17925231}. Note=Cytoplasmic in interphase cells (By similarity). Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1 (By similarity). {ECO:0000250|UniProtKB:O60566}.
uniprot
proteins