Dhfr
entrez
13361
canonical
uniprot
P00375
canonical
ensembl
ENSMUSG00000021707
resolver
ensp
ENSMUSP00000022218
resolver
enst
ENSMUST00000022218
resolver
genesymbol
Dhfr
resolver
uniprot
P70693
resolver
uniprot
Q544T5
resolver
uniprot
Q61485
resolver
uniprot
Q61487
resolver
uniprot
Q61579
resolver
uniprot_entry
DYR_MOUSE
resolver
uniprot_entry
Q544T5_MOUSE
resolver
uniprot_sec
P70693
resolver
uniprot_sec
Q61485
resolver
uniprot_sec
Q61487
resolver
uniprot_sec
Q61579
resolver
amino_acid_sequence
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKKD
uniprot
proteins
ec_number
1.5.1.3
uniprot
proteins
function
FUNCTION: Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner (PubMed:19748785, PubMed:25980602). Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway (PubMed:25980602). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (PubMed:25980602). Binds its own mRNA and that of DHFR2 (By similarity). {ECO:0000250|UniProtKB:P00374, ECO:0000269|PubMed:19748785, ECO:0000269|PubMed:25980602}.
uniprot
proteins
mass_dalton
21606
uniprot
proteins
pathway_participation
PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. {ECO:0000250|UniProtKB:P00374}.
uniprot
proteins
post_translational_modification
PTM: Sumoylated by UBE2I/UBC9. {ECO:0000250|UniProtKB:P00374}.
uniprot
proteins
protein_family
Dihydrofolate reductase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15681865
uniprot
proteins
pubmed
17019704
uniprot
proteins
pubmed
18703847
uniprot
proteins
pubmed
19748785
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
2263462
uniprot
proteins
pubmed
23806337
uniprot
proteins
pubmed
25980602
uniprot
proteins
pubmed
2982814
uniprot
proteins
pubmed
360074
uniprot
proteins
pubmed
6121807
uniprot
proteins
pubmed
6244105
uniprot
proteins
pubmed
6282858
uniprot
proteins
pubmed
6573667
uniprot
proteins
pubmed
762074
uniprot
proteins
sequence_length
187
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:25980602}. Cytoplasm {ECO:0000269|PubMed:25980602}. Nucleus {ECO:0000250|UniProtKB:P00374}. Note=Localized to the nucleus during S and G2/M phase sof the cell cycle. As a component of the de novo thymidylate synthesis complex, localizes specifically to replication forks during DNA synthesis (By similarity). {ECO:0000250|UniProtKB:P00374}.
uniprot
proteins