Tmem97
entrez
69071
canonical
uniprot
Q8VD00
canonical
ensembl
ENSMUSG00000037278
resolver
ensp
ENSMUSP00000099532
resolver
enst
ENSMUST00000103242
resolver
genesymbol
Tmem97
resolver
genesymbol_syn
S2r
resolver
uniprot_entry
SGMR2_MOUSE
resolver
amino_acid_sequence
MGALAARRCVEWLLGLYFVSHIPITLFIDLQAVLPPELYPQEFSNLLRWYSKEFKDPLMQEPPVWFKSFLLCELVFQLPFFPIAAYAFFKGSCRWIRIPAIIYAAHTITTLIPILYTLLFEDFSKAVAFKGQRPESFRERLTLVGVYAPYLIIPLILLLFMLRNPYYKYEEKRKKK
uniprot
proteins
function
FUNCTION: Sigma-2 receptor implicated in the regulation of multiple cellular processes, including cholesterol biosynthesis and trafficking, membrane trafficking, autophagy, lipid membrane-associated protein trafficking, and stabilization of receptors at the cell surface, thereby contributing to the maintenance of cellular homeostasis. Forms a ternary complex with PGRMC1 and LDLR at the plasma membrane, promoting LDLR-mediated internalization of LDL cholesterol. Reduces the availability of the lysosomal sterol transporter NPC1, likely through direct interaction, thereby regulating lipid transport, including cholesterol and LBPA, outside of late endosome/lysosome (By similarity). Binds the regio- and stereoselective oxysterol 20(S)-hydroxycholesterol (20(S)-OHC) (PubMed:34799735). Ligand binding enhances association with NPC1 and modulates TMEM97-containing protein complexes involving PGRMC1 and TSPO, consistent with a role in cholesterol homeostasis (By similarity). Also able to bind cholesterol (By similarity). Interacts with the salivary peptide histatin 1 (Hst 1/HTN1) at the ER membrane, which is critical for increasing mitochondria-ER contacts and stimulating Hst 1 wound healing properties. May alter the activity of some cytochrome P450 proteins. Although sharing homology with sterol isomerases through the EXPERA domain, lacks sterol isomerase activity; instead, may function as a sterol or oxysterol sensor (By similarity). Acts as an ER quality control factor by promoting proteolytic degradation of nonproductive and extramitochondrial precursor proteins in the ER membrane, thereby removing them from the ER surface (By similarity). {ECO:0000250|UniProtKB:Q3MHW7, ECO:0000250|UniProtKB:Q5BJF2, ECO:0000269|PubMed:34799735}.
uniprot
proteins
mass_dalton
20811
uniprot
proteins
mutagenesis
MUTAGEN 61; /note="E->A: No change in 20(S)-OHC binding."; /evidence="ECO:0000269|PubMed:34799735"; MUTAGEN 88; /note="F->A: Loss of protein synthesis."; /evidence="ECO:0000269|PubMed:34799735"; MUTAGEN 95; /note="W->A: No change in 20(S)-OHC binding."; /evidence="ECO:0000269|PubMed:34799735"; MUTAGEN 150; /note="Y->A: Loss of 20(S)-OHC binding."; /evidence="ECO:0000269|PubMed:34799735"; MUTAGEN 150; /note="Y->F: Decreased 20(S)-OHC binding; loss of 20(S)-OHC epimer stereoselectivity."; /evidence="ECO:0000269|PubMed:34799735"; MUTAGEN 150; /note="Y->S: Loss of 20(S)-OHC binding; loss of 20(S)-OHC epimer stereoselectivity."; /evidence="ECO:0000269|PubMed:34799735
uniprot
proteins
protein_family
TMEM97/sigma-2 receptor family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
34799735
uniprot
proteins
sequence_length
176
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Rough endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q5BJF2}; Multi-pass membrane protein {ECO:0000255}. Nucleus membrane {ECO:0000250|UniProtKB:Q5BJF2}; Multi-pass membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 10..30; /note="Helical; Name=1"; /evidence="ECO:0000255"; TRANSMEM 69..89; /note="Helical; Name=2"; /evidence="ECO:0000255"; TRANSMEM 100..120; /note="Helical; Name=3"; /evidence="ECO:0000255"; TRANSMEM 141..161; /note="Helical; Name=4"; /evidence="ECO:0000255
uniprot
proteins