Clec12a
entrez
232413
canonical
uniprot
Q504P2
canonical
ensembl
ENSMUSG00000053063
resolver
ensp
ENSMUSP00000063627
resolver
enst
ENSMUST00000065289
resolver
genesymbol
Clec12a
resolver
genesymbol_syn
Micl
resolver
genesymbol_syn
klrl1
resolver
uniprot
A0A0R4J0T2
resolver
uniprot
Q8BN96
resolver
uniprot_entry
A0A0R4J0T2_MOUSE
resolver
uniprot_entry
CL12A_MOUSE
resolver
uniprot_sec
Q8BN96
resolver
amino_acid_sequence
MSEEIVYANLKIQDPDKKEETQKSDKCGGKVSADASHSQQKTVLILILLCLLLFIGMGVLGGIFYTTLATEMIKSNQLQRAKEELQENVFLQLKHNLNSSKKIKNLSAMLQSTATQLCRELYSKEPEHKCKPCPKGSEWYKDSCYSQLNQYGTWQESVMACSARNASLLKVKNKDVLEFIKYKKLRYFWLALLPRKDRTQYPLSEKMFLSEESERSTDDIDKKYCGYIDRVNVYYTYCTDENNIICEETASKVQLESVLNGLPEDSR
uniprot
proteins
function
FUNCTION: Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation (PubMed:14739280, PubMed:15238421, PubMed:18350551, PubMed:24631154, PubMed:39143217). Myeloid cell inhibition is required to limit proinflammatory pathways and protect against excessive inflammation (PubMed:26275430, PubMed:39143217). Specifically recognizes and binds various structures, such as neutrophil extracellular traps (NETs) or monosodium urate crystals (PubMed:39143217). Also acts as a pattern-recognition receptor for pathogen-associated molecules, such as plasmodium hemozoin or mycobacterial micolic acid (PubMed:31269448, PubMed:36542980). Ligand-binding induces phosphorylation of its ITIM motif, followed by recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11, which counteract tyrosine-protein kinase SYK, thereby preventing myeloid cell activation (PubMed:18350551, PubMed:24631154, PubMed:39143217). Acts as a pattern-recognition receptor for NETs in neutrophils: specifically recognizes DNA in NETs, leading to inhibit neutrophil activation and limit further NET formation (PubMed:39143217). This regulation is essential for controlling key neutrophil responses and limit NET-mediated inflammatory conditions (PubMed:39143217). Also recognizes dead cells by acting as a receptor for monosodium urate crystals, leading to down-regulate neutrophil activation (PubMed:24631154). Binding to monosodium urate crystals also promotes the type I interferon response (PubMed:31451663). Acts as an inhibitor of natural killer (NK) cell cytotoxicity (By similarity). Also acts as an inhibitor of dendritic cell maturation in an IL10-dependent manner (PubMed:30787988). {ECO:0000250|UniProtKB:Q5QGZ9, ECO:0000269|PubMed:14739280, ECO:0000269|PubMed:15238421, ECO:0000269|PubMed:18350551, ECO:0000269|PubMed:24631154, ECO:0000269|PubMed:26275430, ECO:0000269|PubMed:30787988, ECO:0000269|PubMed:31269448, ECO:0000269|PubMed:31451663, ECO:0000269|PubMed:36542980, ECO:0000269|PubMed:39143217}.
uniprot
proteins
mass_dalton
30757
uniprot
proteins
mutagenesis
MUTAGEN 7; /note="Y->F: Abolished phosphorylation."; /evidence="ECO:0000269|PubMed:34638548"; MUTAGEN 118; /note="C->A: Decreased localization to the plasma membrane."; /evidence="ECO:0000269|PubMed:34638548"; MUTAGEN 130; /note="C->A: Does not affect cell-surface expression but increases its oligomerization."; /evidence="ECO:0000269|PubMed:34638548
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Tyr-7 by SRC in the ITIM motif following ligand-binding, promoting recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11. {ECO:0000269|PubMed:31451663, ECO:0000269|PubMed:34638548}.
uniprot
proteins
pubmed
14739280
uniprot
proteins
pubmed
15238421
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
18350551
uniprot
proteins
pubmed
24631154
uniprot
proteins
pubmed
26275430
uniprot
proteins
pubmed
30787988
uniprot
proteins
pubmed
31269448
uniprot
proteins
pubmed
31451663
uniprot
proteins
pubmed
34638548
uniprot
proteins
pubmed
36542980
uniprot
proteins
pubmed
39143217
uniprot
proteins
sequence_length
267
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:34638548}; Single-pass type II membrane protein {ECO:0000255}. Note=Ligand binding leads to internalization. {ECO:0000250|UniProtKB:Q5QGZ9}.
uniprot
proteins
transmembrane_region
TRANSMEM 44..64; /note="Helical; Signal-anchor for type II membrane protein"; /evidence="ECO:0000255
uniprot
proteins