ACAP1
entrez
9744
canonical
uniprot
Q15027
canonical
ensembl
ENSG00000072818
resolver
ensembl
ENSG00000288169
resolver
ensp
ENSP00000158762
resolver
ensp
ENSP00000499859
resolver
ensp
ENSP00000581097
resolver
ensp
ENSP00000735630
resolver
enst
ENST00000158762
resolver
enst
ENST00000672212
resolver
enst
ENST00000911038
resolver
genesymbol
ACAP1
resolver
genesymbol_syn
CENTB1
resolver
genesymbol_syn
KIAA0050
resolver
hgnc
HGNC:16467
resolver
uniprot
Q53XN9
resolver
uniprot_entry
ACAP1_HUMAN
resolver
uniprot_sec
Q53XN9
resolver
activity_regulation
ACTIVITY REGULATION: GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid. {ECO:0000269|PubMed:11062263}.
uniprot
proteins
amino_acid_sequence
MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL
uniprot
proteins
function
FUNCTION: GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. {ECO:0000269|PubMed:11062263, ECO:0000269|PubMed:16256741, ECO:0000269|PubMed:17398097, ECO:0000269|PubMed:17664335, ECO:0000269|PubMed:22645133}.
uniprot
proteins
mass_dalton
81536
uniprot
proteins
mutagenesis
MUTAGEN 14; /note="S->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 29; /note="S->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 274; /note="K->N: Loss of binding to PIP2 and PIP3. Loss of association with endosomal tubules when coexpressed with PIP5K1C."; /evidence="ECO:0000269|PubMed:17010122"; MUTAGEN 277; /note="S->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 280; /note="F->A: Reduced membrane binding and ability to induce liposome tubulation."; /evidence="ECO:0000269|PubMed:25284369"; MUTAGEN 280; /note="F->E: Almost abolishes membrane binding."; /evidence="ECO:0000269|PubMed:25284369"; MUTAGEN 280; /note="F->W: Preserves membrane binding and ability to tubulate liposomes."; /evidence="ECO:0000269|PubMed:25284369"; MUTAGEN 289; /note="T->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 358; /note="S->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 389; /note="T->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 448; /note="R->Q: Loss of GAP activity. No effect on GULP1 binding or association with endosomal tubules when coexpressed with PIP5K1C."; /evidence="ECO:0000269|PubMed:11062263, ECO:0000269|PubMed:17398097"; MUTAGEN 461; /note="T->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 554; /note="S->A: Loss of phosphorylation by PKB, interaction with ITGB1 and ITGB1-dependent cell migration."; /evidence="ECO:0000269|PubMed:16256741, ECO:0000269|PubMed:22645133"; MUTAGEN 554; /note="S->D: Enhances interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741, ECO:0000269|PubMed:22645133"; MUTAGEN 568; /note="S->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 711; /note="T->A: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 712; /note="Y->F: No effect on interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 724; /note="S->A: Loss of phosphorylation at S-554, interaction with ITGB1 and ITGB1-dependent cell migration."; /evidence="ECO:0000269|PubMed:16256741"; MUTAGEN 724; /note="S->D: Enhances interaction with ITGB1."; /evidence="ECO:0000269|PubMed:16256741
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. {ECO:0000269|PubMed:16256741}.
uniprot
proteins
pubmed
11062263
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16256741
uniprot
proteins
pubmed
16777052
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
17010122
uniprot
proteins
pubmed
17398097
uniprot
proteins
pubmed
17664335
uniprot
proteins
pubmed
22645133
uniprot
proteins
pubmed
25284369
uniprot
proteins
pubmed
7584044
uniprot
proteins
sequence_length
740
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Recycling endosome membrane {ECO:0000269|PubMed:16256741}; Peripheral membrane protein {ECO:0000269|PubMed:16256741}; Cytoplasmic side {ECO:0000269|PubMed:16256741}.
uniprot
proteins