Ctps1
entrez
51797
canonical
uniprot
P70698
canonical
ensembl
ENSMUSG00000028633
resolver
ensp
ENSMUSP00000030381
resolver
ensp
ENSMUSP00000205523
resolver
enst
ENSMUST00000030381
resolver
genesymbol
Ctps1
resolver
genesymbol_syn
Ctps
resolver
uniprot
Q922Y4
resolver
uniprot_entry
PYRG1_MOUSE
resolver
uniprot_sec
Q922Y4
resolver
activity_regulation
ACTIVITY REGULATION: Polymerizes into filaments upon substrate-binding, increasing the CTP synthase activity. In contrast to CTPS2, only weakly inhibited by CTP; CTP synthase activity at higher CTP concentrations is consistent with its critical role in expanding nucleotide pools in proliferating cells. Activated by GTP. {ECO:0000250|UniProtKB:P17812}.
uniprot
proteins
amino_acid_sequence
MKYILVTGGVISGIGKGVIASSVGTILKSCGLHVTSIKIDPYINIDAGTFSPYEHGEVFVLDDGGEVDLDLGNYERFLDIRLTKDNNLTTGKIYQYVINKERKGDYLGKTVQVVPHITDAIQEWVMRQALIPVDEDGLEPQVCVIELGGTVGDIESMPFIEAFRQFQFKVKRENFCNIHVSLVPQPSSTGEQKTKPTQNSVRELRGLGLSPDLVVCRCSNPLDTSVKEKISMFCHVEPEQVICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQSRKMLMKWKEMADRYDRLLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSTDLEPSTLQEEPVRYHEAWQKLCSAHGVLVPGGFGVRGTEGKIQAIAWARKQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPKTSHPVVIDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDTDYLEERHRHRFEVNPVLKKCLEEQGLKFVGQDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLPHYLQKGCRLSPRDTYSDRSGSSSPDSEITELKFPSISQD
uniprot
proteins
ec_number
3.5.1.-
uniprot
proteins
ec_number
6.3.4.2
uniprot
proteins
function
FUNCTION: CTP synthase involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids (PubMed:38438357). Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen (By similarity). CTPS1 CTP synthase activity plays a crucial role in the proliferation of activated lymphocytes and immunity; additional CTP being required to meet increased demand for DNA, RNA and lipid membrane biosynthesis in proliferating lymphocytes (PubMed:38438357). In addition to CTP synthase activity, also acts as a protein deamidase that catalyzes the side chain deamidation of specific asparagine residues of proteins to aspartate (PubMed:39719712). Acts as a negative regulator of innate immunity by mediating deamidation of 'Asn-85' of IRF3, preventing IRF3 from binding DNA (PubMed:39719712). Facilitates chromatin relaxation in response to DNA damage by mediating deamidation of 'Asn-76' and 'Asn-77' of histone H1, thereby promoting subsequent acetylation of histone H1 at 'Lys-75' (H1K75ac), increasing chromatin accessibility to facilitate the recruitment of DNA repair proteins (By similarity). {ECO:0000250|UniProtKB:P17812, ECO:0000269|PubMed:38438357, ECO:0000269|PubMed:39719712}.
uniprot
proteins
mass_dalton
66682
uniprot
proteins
mutagenesis
MUTAGEN 250; /note="I->T: Abolished inhibition by small compound R80."; /evidence="ECO:0000269|PubMed:34583994"; MUTAGEN 399; /note="C->A: Abolished protein-asparagine deamidase activity; when associated with 526-A--A-528."; /evidence="ECO:0000269|PubMed:39719712"; MUTAGEN 526..528; /note="HPE->APA: Abolished protein-asparagine deamidase activity; when associated with A-399."; /evidence="ECO:0000269|PubMed:39719712"; MUTAGEN 575; /note="S->A: Impaired ability to inhibit IRF3."; /evidence="ECO:0000269|PubMed:39719712
uniprot
proteins
pathway_participation
PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. {ECO:0000269|PubMed:38438357}.
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Thr-455 by PKA and PKC inhibits the CTP synthase activity. Phosphorylation at Ser-462 by PKC increases the CTP synthase activity (By similarity). Phosphorylation by GSK3-beta (GSK3B) inhibits both the CTP synthase and protein deamidase activities (PubMed:39719712). Phosphorylation at Ser-571 by GSK3-beta (GSK3B) constitutes the major phosphorylation site, while phosphorylation at Ser-575 may by required for priming phosphorylation at Ser-571 (By similarity). Phosphorylation at Ser-575 by GSK3-beta (GSK3B) inhibits its ability to mediate deamination of IRF3 (PubMed:39719712). {ECO:0000250|UniProtKB:P17812, ECO:0000269|PubMed:39719712}.
uniprot
proteins
protein_family
CTP synthase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17242355
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
25223282
uniprot
proteins
pubmed
34583994
uniprot
proteins
pubmed
38438357
uniprot
proteins
pubmed
39719712
uniprot
proteins
sequence_length
591
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:25223282}. Nucleus {ECO:0000250|UniProtKB:P17812}. Chromosome {ECO:0000250|UniProtKB:P17812}. Note=Cytoplasmic and nuclear; when active, detected in long filamentous structures named cytoophidium (PubMed:25223282). Enriched on chromatin in response to DNA damage (By similarity). {ECO:0000250|UniProtKB:P17812, ECO:0000269|PubMed:25223282}.
uniprot
proteins