ALKBH2
entrez
121642
canonical
uniprot
Q6NS38
canonical
ensembl
ENSG00000189046
resolver
ensp
ENSP00000343021
resolver
ensp
ENSP00000398181
resolver
ensp
ENSP00000399820
resolver
ensp
ENSP00000478765
resolver
ensp
ENSP00000531432
resolver
ensp
ENSP00000675618
resolver
ensp
ENSP00000675619
resolver
ensp
ENSP00000774087
resolver
genesymbol
ALKBH2
resolver
genesymbol_syn
ABH2
resolver
hgnc
HGNC:32487
resolver
uniprot
A4PET2
resolver
uniprot
Q5XLE3
resolver
uniprot_entry
ALKB2_HUMAN
resolver
uniprot_sec
A4PET2
resolver
uniprot_sec
Q5XLE3
resolver
activity_regulation
ACTIVITY REGULATION: Activated by ascorbate and magnesium ions. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:18519673}.
uniprot
proteins
amino_acid_sequence
MDRFLVKGAQGGLLRKQEEQEPTGEEPAVLGGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADEIFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLSPKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILLTKK
uniprot
proteins
ec_number
1.14.11.33
uniprot
proteins
function
FUNCTION: Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:20714506, PubMed:23972994, PubMed:25797601). Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis (PubMed:18432238, PubMed:22659876). Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:20714506, PubMed:23972994, PubMed:25797601, PubMed:26408825). Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription (PubMed:23972994). In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication (PubMed:19736315, PubMed:26408825). {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:18432238, ECO:0000269|PubMed:19736315, ECO:0000269|PubMed:20714506, ECO:0000269|PubMed:22659876, ECO:0000269|PubMed:23972994, ECO:0000269|PubMed:25797601, ECO:0000269|PubMed:26408825}.
uniprot
proteins
mass_dalton
29322
uniprot
proteins
mutagenesis
MUTAGEN 3; /note="R->K: Impairs PCNA-binding. No effect on PCNA-binding; when associated with R-7."; /evidence="ECO:0000269|PubMed:19736315"; MUTAGEN 4; /note="F->A: Complete loss of PCNA-binding."; /evidence="ECO:0000269|PubMed:19736315, ECO:0000269|PubMed:26408825"; MUTAGEN 4; /note="F->Y: No effect on PCNA-binding."; /evidence="ECO:0000269|PubMed:19736315"; MUTAGEN 5..6; /note="LV->AA: Strong decrease in PCNA-binding."; /evidence="ECO:0000269|PubMed:19736315"; MUTAGEN 7; /note="K->R: No effect on PCNA-binding; when associated with K-3."; /evidence="ECO:0000269|PubMed:19736315"; MUTAGEN 38..40; /note="RKR->AAA: Leads to cytoplasmic relocalization."; /evidence="ECO:0000269|PubMed:23972994"; MUTAGEN 101..103; /note="VFG->RED: Strong decrease of activity toward N1-methyladenine adduct in both ssDNA and dsDNA substrates."; /evidence="ECO:0000269|PubMed:20714506"; MUTAGEN 101; /note="V->A: Decreases activity toward N1-methyladenine adduct in ssDNA. Has no effect on lesion repair in dsDNA."; /evidence="ECO:0000269|PubMed:20714506"; MUTAGEN 101; /note="V->G: Loss of activity toward N1-methyladenine adduct in either ssDNA or dsDNA; when associated with A-102."; /evidence="ECO:0000269|PubMed:22659876"; MUTAGEN 102; /note="F->A: Strong decrease of activity toward N1-methyladenine adduct. Loss of activity toward N1-methyladenine adduct in either ssDNA or dsDNA; when associated with G-101."; /evidence="ECO:0000269|PubMed:20714506, ECO:0000269|PubMed:22659876"; MUTAGEN 110; /note="R->A: Loss of activity toward N1-methyladenine adduct in either ssDNA or dsDNA."; /evidence="ECO:0000269|PubMed:20714506"; MUTAGEN 122; /note="Y->A: Decreases activity toward N1-methyladenine adduct in either ssDNA or dsDNA."; /evidence="ECO:0000269|PubMed:20714506"; MUTAGEN 124; /note="F->A: Loss of activity toward N1-methyladenine adduct in either ssDNA or dsDNA."; /evidence="ECO:0000269|PubMed:20714506"; MUTAGEN 125; /note="S->A: Strong decrease of activity toward N1-methyladenine adduct in ssDNA. Has no effect on lesion repair in dsDNA."; /evidence="ECO:0000269|PubMed:20714506"; MUTAGEN 173; /note="D->A: Loss of activity associated with decreased rDNA transcription."; /evidence="ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:23972994"; MUTAGEN 175; /note="E->A: Loss of activity."; /evidence="ECO:0000269|PubMed:20714506"; MUTAGEN 236; /note="H->A: Decreases activity."; /evidence="ECO:0000269|PubMed:16174769
uniprot
proteins
protein_family
AlkB family
uniprot
proteins
pubmed
12486230
uniprot
proteins
pubmed
12594517
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16174769
uniprot
proteins
pubmed
16541075
uniprot
proteins
pubmed
17979886
uniprot
proteins
pubmed
18432238
uniprot
proteins
pubmed
18519673
uniprot
proteins
pubmed
19736315
uniprot
proteins
pubmed
20223766
uniprot
proteins
pubmed
20714506
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22659876
uniprot
proteins
pubmed
23972994
uniprot
proteins
pubmed
25797601
uniprot
proteins
pubmed
26408825
uniprot
proteins
sequence_length
261
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:19736315}. Nucleus, nucleolus {ECO:0000269|PubMed:23972994}. Nucleus, nucleoplasm {ECO:0000269|PubMed:23972994}. Note=Relocates to the replication foci during S-phase. {ECO:0000269|PubMed:12594517}.
uniprot
proteins