Ooep
entrez
67968
canonical
uniprot
Q9CWE6
canonical
ensembl
ENSMUSG00000032346
resolver
ensp
ENSMUSP00000034900
resolver
enst
ENSMUST00000034900
resolver
genesymbol
Ooep
resolver
genesymbol_syn
Oep19
resolver
genesymbol_syn
Sddr
resolver
uniprot_entry
OOEP_MOUSE
resolver
amino_acid_sequence
MASHTADADAKPDSDSQKLLNVLPVSLRLRTRPWWFPIQEVSNPLVLYMEAWVAERVIGTDQAEISEIEWMCQALLTVDSVNSGNLAEITIFGQPSAQTRMKNILLNMAAWHKENELQRAVKVKEVEEFLKIRASSILSKLSKKGLKLAGFPLPLEGRETQMES
uniprot
proteins
function
FUNCTION: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (PubMed:18804437, PubMed:25208553, PubMed:37922900). The SCMC complex is a structural constituent of cytoplasmic lattices, which consist in fibrous structures found in the cytoplasm of oocytes and preimplantation embryos (PubMed:31575650, PubMed:37922900, PubMed:41845018, PubMed:41917274, PubMed:41986725). They are required to store maternal proteins critical for embryonic development, such as ribosomal proteins, cytoskeletal tubulins and proteins controlling epigenetic reprogramming of the preimplantation embryo, in a poised but restrained state, regulating their availability for early embryonic development (PubMed:37922900, PubMed:41845018, PubMed:41917274, PubMed:41986725). Cytoplasmic lattices also tightly control ubiquitination during the oocyte-to-embryo transition by trapping E3 ubiquitin-protein ligase UHRF1 in a compact autoinhibited conformation and maintaining SCF ubiquitin ligase complexes inactive (PubMed:41845018, PubMed:41917274, PubMed:41986725). As part of the OOEP-KHDC3 scaffold, recruits BLM and TRIM25 to DNA replication forks, thereby promoting the ubiquitination of BLM by TRIM25, enhancing BLM retainment at replication forks and therefore promoting stalled replication fork restart (PubMed:29125140). Positively regulates the homologous recombination-mediated DNA double-strand break (DSB) repair pathway by regulating ATM activation and RAD51 recruitment to DSBs in oocytes (PubMed:29955025). Thereby contributes to oocyte survival and the resumption and completion of meiosis (PubMed:29955025). {ECO:0000269|PubMed:18804437, ECO:0000269|PubMed:25208553, ECO:0000269|PubMed:29125140, ECO:0000269|PubMed:29955025, ECO:0000269|PubMed:31575650, ECO:0000269|PubMed:37922900, ECO:0000269|PubMed:41845018, ECO:0000269|PubMed:41917274, ECO:0000269|PubMed:41986725}.
uniprot
proteins
mass_dalton
18460
uniprot
proteins
mutagenesis
MUTAGEN 32; /note="R->W,P,G: Impaired formation of the subcortical maternal complex (SCMC)."; /evidence="ECO:0000269|PubMed:38177687
uniprot
proteins
protein_family
KHDC1 family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
18804437
uniprot
proteins
pubmed
25208553
uniprot
proteins
pubmed
28992324
uniprot
proteins
pubmed
29125140
uniprot
proteins
pubmed
29955025
uniprot
proteins
pubmed
31575650
uniprot
proteins
pubmed
37922900
uniprot
proteins
pubmed
38177687
uniprot
proteins
pubmed
41118754
uniprot
proteins
pubmed
41845018
uniprot
proteins
pubmed
41917274
uniprot
proteins
pubmed
41986725
uniprot
proteins
sequence_length
164
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18804437, ECO:0000269|PubMed:37922900}. Nucleus {ECO:0000269|PubMed:29125140}. Note=Core component of cytoplasmic lattices in oocytes (PubMed:37922900). In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages (PubMed:18804437, PubMed:25208553). From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions (PubMed:18804437). Expression largely disappears in blastocysts (PubMed:25208553). {ECO:0000269|PubMed:18804437, ECO:0000269|PubMed:25208553, ECO:0000269|PubMed:37922900}.
uniprot
proteins