RNF186
entrez
54546
canonical
uniprot
Q9NXI6
canonical
ensembl
ENSG00000178828
resolver
ensp
ENSP00000364263
resolver
enst
ENST00000375121
resolver
genesymbol
RNF186
resolver
hgnc
HGNC:25978
resolver
uniprot
Q53GE0
resolver
uniprot_entry
RN186_HUMAN
resolver
uniprot_sec
Q53GE0
resolver
amino_acid_sequence
MACTKTLQQSQPISAGATTTTTAVAPAGGHSGSTECDLECLVCREPYSCPRLPKLLACQHAFCAICLKLLLCVQDNTWSITCPLCRKVTAVPGGLICSLRDHEAVVGQLAQPCTEVSLCPQGLVDPADLAAGHPSLVGEDGQDEVSANHVAARRLAAHLLLLALLIILIGPFIYPGVLRWVLTFIIALALLMSTLFCCLPSTRGSCWPSSRTLFCREQKHSHISSIA
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
function
FUNCTION: E3 ubiquitin protein ligase that is part of an apoptotic signaling pathway activated by endoplasmic reticulum stress (PubMed:23896122). Stimulates the expression of proteins specific of the unfolded protein response (UPR), ubiquitinates BNIP1 and regulates its localization to the mitochondrion and induces calcium release from the endoplasmic reticulum that ultimately leads to cell apoptosis (PubMed:23896122). Plays a role in the maintenance of intestinal homeostasis and clearance of enteric pathogens. Upon NOD2 stimulation, ubiquitinates the ER stress sensor activating transcription factor 6/ATF6 and promotes the unfolded protein response UPR (PubMed:34623328). Participates in basal level of autophagy maintenance by regulating the ubiquitination of EPHB2 and EPHB3. Upon stimulation by ligand EFNB1, ubiquitinates EPHB2 and further recruits MAP1LC3B for autophagy induction (PubMed:33280498). Controls nutrient sensing by ubiquitinating Sestrin-2/SESN2, which is an intracellular sensor of cytosolic leucine and inhibitor of mTORC1 activity (PubMed:31586034). {ECO:0000269|PubMed:23896122, ECO:0000269|PubMed:31586034, ECO:0000269|PubMed:33280498, ECO:0000269|PubMed:34623328}.
uniprot
proteins
mass_dalton
24145
uniprot
proteins
mutagenesis
MUTAGEN 58..66; /note="CQHAFCAIC->AQWAFAAIA: Loss of autoubiquitination; loss of BNIP1 polyubiquitination; no effect on interaction with BNIP1; decreased effect on calcium release from the endoplasmic reticulum."; /evidence="ECO:0000269|PubMed:23896122"; MUTAGEN 60; /note="H->W: Loss of ability to decrease SESN2 protein."; /evidence="ECO:0000269|PubMed:31586034"; MUTAGEN 64; /note="A->T: Exhibits a reduced ability to ubiquitinate EPHB2 but retained ability to ubiquitinate EPHB3."; /evidence="ECO:0000269|PubMed:33280498
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:23896122}.
uniprot
proteins
post_translational_modification
PTM: Polyubiquitinated. 'Lys-29'-linked autoubiquitination leads to proteasomal degradation. {ECO:0000269|PubMed:23896122}.
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16710414
uniprot
proteins
pubmed
23896122
uniprot
proteins
pubmed
31586034
uniprot
proteins
pubmed
33280498
uniprot
proteins
pubmed
34623328
uniprot
proteins
sequence_length
227
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:23896122, ECO:0000269|PubMed:34623328}; Multi-pass membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 158..178; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 180..200; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins