Pias1
entrez
56469
canonical
uniprot
O88907
canonical
ensembl
ENSMUSG00000032405
resolver
ensp
ENSMUSP00000096248
resolver
enst
ENSMUST00000098651
resolver
genesymbol
Pias1
resolver
genesymbol_syn
Ddxbp1
resolver
uniprot
Q8C6H5
resolver
uniprot_entry
PIAS1_MOUSE
resolver
uniprot_sec
Q8C6H5
resolver
amino_acid_sequence
MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVIDLTIDSSSDEEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSSGSNSSLVSSNSLRESHGHGVASRSSADTASIFGIIPDIISLD
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
function
FUNCTION: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed:24061474). Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2, PML and ZNF76 (PubMed:24061474). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway (By similarity). In vitro, binds A/T-rich DNA (By similarity). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context (By similarity). Mediates sumoylation of MRE11, stabilizing MRE11 on chromatin during end resection (By similarity). Sumoylates PML (at 'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation (PubMed:22406621). PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (PubMed:22406621). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (By similarity). Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (PubMed:24061474). Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (PubMed:16600910). Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumoylation activity (PubMed:16600910). Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter (PubMed:16600910). {ECO:0000250|UniProtKB:O75925, ECO:0000269|PubMed:16600910, ECO:0000269|PubMed:22406621, ECO:0000269|PubMed:24061474}.
uniprot
proteins
mass_dalton
71618
uniprot
proteins
mutagenesis
MUTAGEN 346; /note="C->G: Loss of promotion of EKLF sumoylation; when associated with G-351 and A-356."; /evidence="ECO:0000269|PubMed:17938210"; MUTAGEN 351; /note="C->G: Loss of promotion of EKLF sumoylation; when associated with G-346 and A-356."; /evidence="ECO:0000269|PubMed:17938210"; MUTAGEN 351; /note="C->S: No effect on interaction with MSX1 or localization of either protein at the nuclear periphery."; /evidence="ECO:0000269|PubMed:16600910"; MUTAGEN 356; /note="C->A: Loss of promotion of EKLF sumoylation; when associated with G-346 and G-351."; /evidence="ECO:0000269|PubMed:17938210"; MUTAGEN 363; /note="I->S: Loss of promotion of EKLF sumoylation."; /evidence="ECO:0000269|PubMed:17938210"; MUTAGEN 372; /note="W->A: Loss of promotion of NCOA2 sumoylation. No effect on interaction with MSX1 or localization of either protein at the nuclear periphery."; /evidence="ECO:0000269|PubMed:12077349, ECO:0000269|PubMed:16600910"; MUTAGEN 375..376; /note="PV->AA: Loss of promotion of EKLF sumoylation."; /evidence="ECO:0000269|PubMed:17938210
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein sumoylation.
uniprot
proteins
post_translational_modification
PTM: Sumoylated. {ECO:0000250|UniProtKB:O75925}.
uniprot
proteins
protein_family
PIAS family
uniprot
proteins
pubmed
10854042
uniprot
proteins
pubmed
11117529
uniprot
proteins
pubmed
12077349
uniprot
proteins
pubmed
12855578
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16600910
uniprot
proteins
pubmed
17187077
uniprot
proteins
pubmed
17242355
uniprot
proteins
pubmed
17938210
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22406621
uniprot
proteins
pubmed
24061474
uniprot
proteins
pubmed
9724754
uniprot
proteins
sequence_length
651
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16600910}. Nucleus speckle {ECO:0000250|UniProtKB:O75925}. Nucleus, PML body {ECO:0000269|PubMed:12077349, ECO:0000269|PubMed:22406621}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:O75925}. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton (By similarity). Interaction with MSX1 is required for localization to the nuclear periphery (PubMed:16600910). {ECO:0000250|UniProtKB:O75925, ECO:0000269|PubMed:16600910}.
uniprot
proteins