Gne
entrez
50798
canonical
uniprot
Q91WG8
canonical
ensembl
ENSMUSG00000028479
resolver
ensp
ENSMUSP00000030201
resolver
ensp
ENSMUSP00000100000
resolver
enst
ENSMUST00000030201
resolver
enst
ENSMUST00000102936
resolver
genesymbol
Gne
resolver
genesymbol_syn
Glcne
resolver
genesymbol_syn
Uae1
resolver
uniprot
F8WH91
resolver
uniprot
Q3TCI8
resolver
uniprot
Q3UW64
resolver
uniprot
Q8CC83
resolver
uniprot
Q8CCB0
resolver
uniprot
Q9Z0P6
resolver
uniprot_entry
F8WH91_MOUSE
resolver
uniprot_entry
GLCNE_MOUSE
resolver
uniprot_entry
Q3TCI8_MOUSE
resolver
uniprot_entry
Q3UW64_MOUSE
resolver
uniprot_sec
Q8CC83
resolver
uniprot_sec
Q8CCB0
resolver
uniprot_sec
Q9Z0P6
resolver
activity_regulation
ACTIVITY REGULATION: The UDP-N-acetylglucosamine 2-epimerase activity, in contrast to the N-acetylmannosamine kinase activity, exhibits allosteric regulation by cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), the end product of neuraminic acid biosynthesis (By similarity). Moreover, the activity is contingent upon the oligomeric state of the enzyme. The monomeric form is inactive, while the dimeric form selectively catalyzes the phosphorylation of N-acetylmannosamine. The hexameric form, on the other hand, demonstrates full proficiency in both enzyme activities (By similarity). Furthermore, the UDP-N-acetylglucosamine 2-epimerase activity is increased by PKC-mediated phosphorylation (PubMed:10745088). {ECO:0000250|UniProtKB:O35826, ECO:0000250|UniProtKB:Q9Y223, ECO:0000269|PubMed:10745088}.
uniprot
proteins
amino_acid_sequence
MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKCKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIH
uniprot
proteins
ec_number
2.7.1.60
uniprot
proteins
ec_number
3.2.1.183
uniprot
proteins
function
FUNCTION: Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis (PubMed:11929971, PubMed:38237079). Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition (By similarity). {ECO:0000250|UniProtKB:Q9Y223, ECO:0000269|PubMed:11929971, ECO:0000269|PubMed:38237079}.
uniprot
proteins
mass_dalton
79199
uniprot
proteins
mutagenesis
MUTAGEN 563; /note="C->Y: Loss-of-function mutant resulting in impaired sialic acid biosynthesis."; /evidence="ECO:0000269|PubMed:38237079"; MUTAGEN 704; /note="P->R: Homozygous embryos exhibit cerebrospinal hemorrhages and defective angiogenesis in the diencephalon at 11 dpc, and become non-viable between 11.5 and 12.5 dpc. Loss-of-function mutant resulting in impaired sialic acid biosynthesis."; /evidence="ECO:0000269|PubMed:38237079
uniprot
proteins
pathway_participation
PATHWAY: Amino-sugar metabolism; N-acetylneuraminate biosynthesis. {ECO:0000269|PubMed:11929971}.
uniprot
proteins
post_translational_modification
PTM: Phosphorylated. Phosphorylation by PKC activates the UDP-N-acetylglucosamine 2-epimerase activity. {ECO:0000269|PubMed:10745088}.
uniprot
proteins
protein_family
UDP-N-acetylglucosamine 2-epimerase family; ROK (NagC/XylR) family
uniprot
proteins
pubmed
10103025
uniprot
proteins
pubmed
10745088
uniprot
proteins
pubmed
11929971
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
38237079
uniprot
proteins
sequence_length
722
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:O35826}.
uniprot
proteins