Napepld
entrez
296757
canonical
uniprot
Q769K2
canonical
ensembl
ENSRNOG00000011363
resolver
ensp
ENSRNOP00000073608
resolver
ensp
ENSRNOP00000108050
resolver
enst
ENSRNOT00000086609
resolver
enst
ENSRNOT00000136190
resolver
genesymbol
Napepld
resolver
uniprot
A0A0G2K5Y7
resolver
uniprot
A0ABK0LBJ3
resolver
uniprot_entry
A0A0G2K5Y7_RAT
resolver
uniprot_entry
A0ABK0LBJ3_RAT
resolver
uniprot_entry
NAPEP_RAT
resolver
activity_regulation
ACTIVITY REGULATION: Activated by divalent cations (PubMed:17655883). Activated by bile acids (By similarity). Activated by membrane phospholipids such as phosphatidylethanolamines. Inhibited by cardiolipins (PubMed:17655883). {ECO:0000250|UniProtKB:Q6IQ20, ECO:0000269|PubMed:17655883}.
uniprot
proteins
amino_acid_sequence
MDENENSQSPAPSHQYPKETLRKRQNSVQNSGGSESSRLSRKSFKLDYRLEEDVTKSKKGKDGRFVNPWPTWKNVSIPNVLRWLIMEKDHSSVPGSKEELDKELPVLKPYFISDPEEAGVREAGLRVTWLGHATLMVEMDELILLTDPMFSSRASPSQYMGPKRFRRPPCTISELPPIDAVLISHNHYDHLDYGSVLALNERFGSELRWFVPLGLLDWMQKCGCENVIELDWWEENCVPGHDKVTFVFTPSQHWCKRTLLDDNKVLWGSWSVLGPWNRFFFAGDTGYCPAFEEIGKRFGPFDLAAIPIGAYEPRWFMKYQHADPEDAVRIHIDVQAKRSVAIHWGTFALANEHYLEPPVKLNEALERYGLKSEDFFILKHGESRYLNTDDKAFEET
uniprot
proteins
ec_number
3.1.4.54
uniprot
proteins
function
FUNCTION: D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid (PubMed:14634025, PubMed:16527816, PubMed:17655883). Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain (By similarity). May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation (By similarity). As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs (By similarity). {ECO:0000250|UniProtKB:Q8BH82, ECO:0000269|PubMed:14634025, ECO:0000269|PubMed:16527816, ECO:0000269|PubMed:17655883}.
uniprot
proteins
mass_dalton
45723
uniprot
proteins
mutagenesis
MUTAGEN 147; /note="D->N: Abolishes activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 170; /note="C->S: Small decrease in activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 185; /note="H->N: <1% of wild-type activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 187; /note="H->N: <1% of wild-type activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 189; /note="D->N: <1% of wild-type activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 190; /note="H->N: <1% of wild-type activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 207; /note="L->F: <5% of wild-type activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 222; /note="C->S: No effect on activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 224; /note="C->S: Considerable decrease in activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 237; /note="C->S: No effect on activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 253; /note="H->N: Abolishes activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 255; /note="C->S: No effect on activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 284; /note="D->N: Abolishes activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 288; /note="C->S: No effect on activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 321; /note="H->N: Abolishes activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 331; /note="H->N: 4% of wild-type activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 343; /note="H->N: No effect on activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 353; /note="H->N: No effect on activity."; /evidence="ECO:0000269|PubMed:16527816"; MUTAGEN 380; /note="H->R: <5% of wild-type activity."; /evidence="ECO:0000269|PubMed:16527816
uniprot
proteins
protein_family
NAPE-PLD family
uniprot
proteins
pubmed
14634025
uniprot
proteins
pubmed
16527816
uniprot
proteins
pubmed
17655883
uniprot
proteins
sequence_length
396
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q6IQ20}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q6IQ20}. Early endosome membrane {ECO:0000250|UniProtKB:Q6IQ20}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q6IQ20}. Nucleus envelope {ECO:0000250|UniProtKB:Q6IQ20}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q6IQ20}. Note=Localized in the proximity of the cellular membranes likely through interaction with membrane phospholipids. {ECO:0000250|UniProtKB:Q6IQ20}.
uniprot
proteins