Klf4
entrez
16600
canonical
uniprot
Q60793
canonical
ensembl
ENSMUSG00000003032
resolver
ensp
ENSMUSP00000103245
resolver
enst
ENSMUST00000107619
resolver
genesymbol
Klf4
resolver
genesymbol_syn
Ezf
resolver
genesymbol_syn
Gklf
resolver
genesymbol_syn
Zie
resolver
uniprot
P70421
resolver
uniprot
Q3U2D6
resolver
uniprot
Q3URS6
resolver
uniprot
Q78K30
resolver
uniprot
Q9R255
resolver
uniprot_entry
KLF4_MOUSE
resolver
uniprot_sec
P70421
resolver
uniprot_sec
Q3U2D6
resolver
uniprot_sec
Q3URS6
resolver
uniprot_sec
Q78K30
resolver
uniprot_sec
Q9R255
resolver
amino_acid_sequence
MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWREELSHMKRLPPLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAASNTGGGLLYSRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAVPSCTVSRSLEAHLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLPPGFHPHPGPNYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF
uniprot
proteins
function
FUNCTION: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216, PubMed:39986490). Binds to the promoter region of its own gene and can activate its own transcription (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Regulates the expression of key transcription factors during embryonic development (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity). {ECO:0000250, ECO:0000269|PubMed:10431239, ECO:0000269|PubMed:10556311, ECO:0000269|PubMed:15358627, ECO:0000269|PubMed:16954384, ECO:0000269|PubMed:17060454, ECO:0000269|PubMed:19816951, ECO:0000269|PubMed:20071344, ECO:0000269|PubMed:29593216}.
uniprot
proteins
mass_dalton
51880
uniprot
proteins
mutagenesis
MUTAGEN 46; /note="E->A: No change in KLF4 polyglutamylation."; /evidence="ECO:0000269|PubMed:29593216"; MUTAGEN 95; /note="E->A: No change in KLF4 polyglutamylation."; /evidence="ECO:0000269|PubMed:29593216"; MUTAGEN 229; /note="K->R: Increased cell reprogramming and pluripotency. No change in promoter-binding of target genes."; /evidence="ECO:0000269|PubMed:29593216"; MUTAGEN 326; /note="E->A: No change in KLF4 polyglutamylation."; /evidence="ECO:0000269|PubMed:29593216"; MUTAGEN 381; /note="E->A: Loss of polyglutamylation, reduced cell reprogramming and pluripotency maintenance. Forms heterodimer with KLF5. No change in promoter-binding of target genes. Embryo lethality in mutant homozygous mice."; /evidence="ECO:0000269|PubMed:29593216
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis. {ECO:0000269|PubMed:29593216}.; PTM: Polyglutamylated by TTLL1 and TTLL4 at Glu-381, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination (PubMed:29593216). Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination (PubMed:29593216). KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis (PubMed:29593216). Ubiquitinated in a FBXO32-dependent manner, leading to proteasomal degradation (PubMed:39986490). {ECO:0000269|PubMed:29593216, ECO:0000269|PubMed:39986490}.
uniprot
proteins
protein_family
Krueppel C2H2-type zinc-finger protein family
uniprot
proteins
pubmed
10431239
uniprot
proteins
pubmed
10556311
uniprot
proteins
pubmed
12441126
uniprot
proteins
pubmed
15358627
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16904174
uniprot
proteins
pubmed
16954384
uniprot
proteins
pubmed
17060454
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
19816951
uniprot
proteins
pubmed
20071344
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
24161396
uniprot
proteins
pubmed
29593216
uniprot
proteins
pubmed
35922041
uniprot
proteins
pubmed
39986490
uniprot
proteins
pubmed
8702718
uniprot
proteins
pubmed
8940147
uniprot
proteins
sequence_length
483
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10431239, ECO:0000269|PubMed:24161396, ECO:0000269|PubMed:35922041, ECO:0000269|PubMed:39986490}. Cytoplasm {ECO:0000269|PubMed:35922041}.
uniprot
proteins