BEST2
entrez
54831
canonical
uniprot
Q8NFU1
canonical
ensembl
ENSG00000039987
resolver
ensp
ENSP00000042931
resolver
ensp
ENSP00000447203
resolver
ensp
ENSP00000448310
resolver
enst
ENST00000042931
resolver
enst
ENST00000549706
resolver
enst
ENST00000553030
resolver
genesymbol
BEST2
resolver
genesymbol_syn
VMD2L1
resolver
hgnc
HGNC:17107
resolver
uniprot
Q53YQ8
resolver
uniprot
Q9NXP0
resolver
uniprot_entry
BEST2_HUMAN
resolver
uniprot_sec
Q53YQ8
resolver
uniprot_sec
Q9NXP0
resolver
activity_regulation
ACTIVITY REGULATION: Chloride channel activity is allosterically inhibited by GLUL/glutamine synthase (GS) which affects the gating at the aperture in the absence of intracellular glutamate (PubMed:36289327). Inhibitory effect of GLUL is relieved upon increasing of L-glutamate intracellular level (PubMed:36289327). {ECO:0000269|PubMed:36289327}.
uniprot
proteins
amino_acid_sequence
MTVTYTARVANARFGGFSQLLLLWRGSIYKLLWRELLCFLGFYMALSAAYRFVLTEGQKRYFEKLVIYCDQYASLIPVSFVLGFYVTLVVNRWWSQYLCMPLPDALMCVVAGTVHGRDDRGRLYRRTLMRYAGLSAVLILRSVSTAVFKRFPTIDHVVEAGFMTREERKKFENLNSSYNKYWVPCVWFSNLAAQARREGRIRDNSALKLLLEELNVFRGKCGMLFHYDWISVPLVYTQVVTIALYSYFLACLIGRQFLDPAQGYKDHDLDLCVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNFQVSMLAVDEMYDDLAVLEKDLYWDAAEARAPYTAATVFQLRQPSFQGSTFDITLAKEDMQFQRLDGLDGPMGEAPGDFLQRLLPAGAGMVAGGPLGRRLSFLLRKNSCVSEASTGASCSCAVVPEGAAPECSCGDPLLDPGLPEPEAPPPAGPEPLTLIPGPVEPFSIVTMPGPRGPAPPWLPSPIGEEEENLA
uniprot
proteins
function
FUNCTION: Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed:11904445, PubMed:18400985, PubMed:32251414, PubMed:35789156, PubMed:36289327). Transports a large specter of anions, namely mediates the movement of chloride, L-glutamate and iodide (PubMed:11904445, PubMed:18400985, PubMed:32251414, PubMed:35789156, PubMed:36289327). Calcium-binding triggers the dilation of the aperture, but calcium-dependent gating is only effective when the size of the passing anion is bigger than the closed aperture (By similarity). Mediates the calcium-activated hydrogencarbonate movement and participates in colonic hydrogencarbonate secretion concomitant with mucin secretion (By similarity). In non-pigmented epithelium (NPE), mediates the efflux of intracellular L-glutamate; binding of intracellular L-glutamate activates and open both the neck and the aperture of the channel, leading to L-glutamate exit promoting chloride influx movement from the extracellular side in trans (PubMed:36289327). Also exhibits a directional permeability for intracellular glutamine, in a similar manner as for L-glutamate (PubMed:36289327). {ECO:0000250|UniProtKB:E1BF86, ECO:0000250|UniProtKB:Q8BGM5, ECO:0000269|PubMed:11904445, ECO:0000269|PubMed:18400985, ECO:0000269|PubMed:32251414, ECO:0000269|PubMed:35789156, ECO:0000269|PubMed:36289327}.
uniprot
proteins
mass_dalton
57139
uniprot
proteins
mutagenesis
MUTAGEN 299; /note="G->A: Does not affect expression level. Does not affect interaction with GLUL. Does not affect tethering of GLUL to the membrane. Impairs channel activity."; /evidence="ECO:0000269|PubMed:36289327"; MUTAGEN 304; /note="D->A: Does not affect expression level. Does not affect interaction with GLUL. Does not affect tethering of GLUL to the membrane. Impairs channel activity."; /evidence="ECO:0000269|PubMed:36289327
uniprot
proteins
protein_family
Anion channel-forming bestrophin (TC 1.A.46) family
uniprot
proteins
protein_family
Calcium-sensitive chloride channel subfamily
uniprot
proteins
pubmed
11904445
uniprot
proteins
pubmed
12032738
uniprot
proteins
pubmed
12907679
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
18400985
uniprot
proteins
pubmed
20407206
uniprot
proteins
pubmed
32251414
uniprot
proteins
pubmed
35789156
uniprot
proteins
pubmed
36289327
uniprot
proteins
sequence_length
509
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:E1BF86}; Multi-pass membrane protein {ECO:0000269|PubMed:35789156}. Basolateral cell membrane {ECO:0000269|PubMed:20407206}; Multi-pass membrane protein.
uniprot
proteins
transmembrane_region
TRANSMEM 32..51; /note="Helical"; /evidence="ECO:0000269|PubMed:35789156, ECO:0007744|PDB:8D1E, ECO:0007744|PDB:8D1F, ECO:0007744|PDB:8D1G, ECO:0007744|PDB:8D1H"; TRANSMEM 61..82; /note="Helical"; /evidence="ECO:0000269|PubMed:35789156, ECO:0007744|PDB:8D1E, ECO:0007744|PDB:8D1F, ECO:0007744|PDB:8D1G, ECO:0007744|PDB:8D1H"; TRANSMEM 239..255; /note="Helical"; /evidence="ECO:0000269|PubMed:35789156, ECO:0007744|PDB:8D1E, ECO:0007744|PDB:8D1F, ECO:0007744|PDB:8D1G, ECO:0007744|PDB:8D1H"; TRANSMEM 275..288; /note="Helical"; /evidence="ECO:0000269|PubMed:35789156, ECO:0007744|PDB:8D1E, ECO:0007744|PDB:8D1F, ECO:0007744|PDB:8D1G, ECO:0007744|PDB:8D1H
uniprot
proteins