EIF5
entrez
1983
canonical
uniprot
P55010
canonical
ensembl
ENSG00000100664
resolver
ensp
ENSP00000216554
resolver
ensp
ENSP00000376477
resolver
ensp
ENSP00000453743
resolver
enst
ENST00000216554
resolver
enst
ENST00000392715
resolver
enst
ENST00000558506
resolver
genesymbol
EIF5
resolver
hgnc
HGNC:3299
resolver
uniprot
Q53XB3
resolver
uniprot
Q9H5N2
resolver
uniprot
Q9UG48
resolver
uniprot_entry
IF5_HUMAN
resolver
uniprot_sec
Q53XB3
resolver
uniprot_sec
Q9H5N2
resolver
uniprot_sec
Q9UG48
resolver
amino_acid_sequence
MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI
uniprot
proteins
function
FUNCTION: Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed:11166181, PubMed:22813744, PubMed:24319994). In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3) (PubMed:11166181). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD) (PubMed:22813744). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC (PubMed:24319994). When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP (PubMed:11166181). Start codon recognition also induces a conformational change of the PIC to a closed state (PubMed:22813744). This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC (PubMed:22813744). Finally, EIF5 stabilizes the PIC in its closed conformation (PubMed:22813744). {ECO:0000269|PubMed:11166181, ECO:0000269|PubMed:22813744, ECO:0000269|PubMed:24319994}.
uniprot
proteins
mass_dalton
49223
uniprot
proteins
mutagenesis
MUTAGEN 15; /note="R->M: Loss of ability to promote hydrolysis of GTP."; /evidence="ECO:0000269|PubMed:11166181"; MUTAGEN 305..306; /note="HN->DD: Disruption of binding to eIF1 and EIF2-beta (EIF2S2); when associated with 347-K-K-348."; /evidence="ECO:0000269|PubMed:22813744"; MUTAGEN 347..348; /note="EE->KK: Disruption of binding to eIF1 and EIF2-beta (EIF2S2); when associated with 305-D-D-306."; /evidence="ECO:0000269|PubMed:22813744
uniprot
proteins
protein_family
EIF-2-beta/eIF-5 family
uniprot
proteins
pubmed
11166181
uniprot
proteins
pubmed
11230166
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
17974005
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
18691976
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
21745818
uniprot
proteins
pubmed
22813744
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
24319994
uniprot
proteins
pubmed
24658146
uniprot
proteins
pubmed
28112733
uniprot
proteins
pubmed
30211544
uniprot
proteins
pubmed
8663286
uniprot
proteins
sequence_length
431
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
uniprot
proteins