TOP1
entrez
7150
canonical
uniprot
P11387
canonical
ensembl
ENSG00000198900
resolver
ensp
ENSP00000354522
resolver
enst
ENST00000361337
resolver
genesymbol
TOP1
resolver
hgnc
HGNC:11986
resolver
uniprot
A8KA78
resolver
uniprot
E1P5W3
resolver
uniprot
O43256
resolver
uniprot
Q12855
resolver
uniprot
Q12856
resolver
uniprot
Q15610
resolver
uniprot
Q5TFY3
resolver
uniprot
Q9BVT2
resolver
uniprot
Q9UJN0
resolver
uniprot_entry
Q9BVT2_HUMAN
resolver
uniprot_entry
TOP1_HUMAN
resolver
uniprot_sec
A8KA78
resolver
uniprot_sec
E1P5W3
resolver
uniprot_sec
O43256
resolver
uniprot_sec
Q12855
resolver
uniprot_sec
Q12856
resolver
uniprot_sec
Q15610
resolver
uniprot_sec
Q5TFY3
resolver
uniprot_sec
Q9UJN0
resolver
activity_regulation
ACTIVITY REGULATION: Specifically inhibited by camptothecin (CPT), a plant alkaloid with antitumor activity.
uniprot
proteins
amino_acid_sequence
MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDAKIKKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPRDEDDADYKPKKIKTEDTKKEKKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF
uniprot
proteins
disease_involvement
DISEASE: Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. {ECO:0000269|PubMed:10556215}.
uniprot
proteins
ec_number
5.6.2.1
uniprot
proteins
function
FUNCTION: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter. {ECO:0000250|UniProtKB:Q13472, ECO:0000269|PubMed:14594810, ECO:0000269|PubMed:16033260, ECO:0000269|PubMed:19168442, ECO:0000269|PubMed:22904072, ECO:0000269|PubMed:2833744}.
uniprot
proteins
mass_dalton
90726
uniprot
proteins
mutagenesis
MUTAGEN 103; /note="K->R: Localizes in both nucleoplasm and nucleoli; when associated with R-117 or R-153. Almost complete loss of sumoylation, concentrates in nucleoli and no clearing from nucleoli on CPT treatment; when associated with R-117 and R-153."; /evidence="ECO:0000269|PubMed:12149243"; MUTAGEN 117; /note="K->R: 5-fold decrease in sumoylation. Localizes in both nucleoplasm and nucleoli; when associated with or without R-103 or R-153. Almost complete loss of sumoylation, concentrates in nucleoli and no clearing from nucleoli on CPT treatment; when associated with R-103 and R-153."; /evidence="ECO:0000269|PubMed:12149243"; MUTAGEN 153; /note="K->R: Localizes in both nucleoplasm and nucleoli; when associated with R-103 or R-117. Almost complete loss of sumoylation, concentrates in nucleoli and no clearing from nucleoli on CPT treatment; when associated with R-103 and R-117."; /evidence="ECO:0000269|PubMed:12149243"; MUTAGEN 532; /note="K->A: Almost abolishes enzyme activity."; /evidence="ECO:0000269|PubMed:14594810"; MUTAGEN 532; /note="K->R: Strongly reduced enzyme activity."; /evidence="ECO:0000269|PubMed:14594810"; MUTAGEN 723; /note="Y->F: No change in CPT-induced clearing from nuclei."; /evidence="ECO:0000269|PubMed:12149243
uniprot
proteins
post_translational_modification
PTM: Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. {ECO:0000269|PubMed:12149243}.; PTM: Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin. {ECO:0000269|PubMed:23185622}.
uniprot
proteins
protein_family
Type IB topoisomerase family
uniprot
proteins
pubmed
10556215
uniprot
proteins
pubmed
10841763
uniprot
proteins
pubmed
11780052
uniprot
proteins
pubmed
12149243
uniprot
proteins
pubmed
12209008
uniprot
proteins
pubmed
12426403
uniprot
proteins
pubmed
12533542
uniprot
proteins
pubmed
1332703
uniprot
proteins
pubmed
14594810
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15165849
uniprot
proteins
pubmed
15801827
uniprot
proteins
pubmed
16033260
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
17081983
uniprot
proteins
pubmed
18003733
uniprot
proteins
pubmed
1849260
uniprot
proteins
pubmed
1851751
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
19168442
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19608861
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
2176592
uniprot
proteins
pubmed
22904072
uniprot
proteins
pubmed
23185622
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
23213405
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
2461859
uniprot
proteins
pubmed
2479024
uniprot
proteins
pubmed
25114211
uniprot
proteins
pubmed
25218447
uniprot
proteins
pubmed
2544263
uniprot
proteins
pubmed
25755297
uniprot
proteins
pubmed
25772364
uniprot
proteins
pubmed
26030138
uniprot
proteins
pubmed
28112733
uniprot
proteins
pubmed
2833744
uniprot
proteins
pubmed
7882333
uniprot
proteins
pubmed
9488644
uniprot
proteins
pubmed
9488652
uniprot
proteins
sequence_length
765
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12149243}. Nucleus, nucleoplasm {ECO:0000269|PubMed:12149243}. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli.
uniprot
proteins