P30099
uniprot
P30099
canonical
amino_acid_sequence
MGACDNDFIELHSRVTADVWLARPWQCLHRTRALGTTATLAPKTLKPFEAIPQYSRNKWLKMIQILREQGQENLHLEMHQAFQELGPIFRHSAGGAQIVSVMLPEDAEKLHQVESILPRRMHLEPWVAHRELRGLRRGVFLLNGAEWRFNRLKLNPNVLSPKAVQNFVPMVDEVARDFLEALKKKVRQNARGSLTMDVQQSLFNYTIEASNFALFGERLGLLGHDLNPGSLKFIHALHSMFKSTTQLLFLPRSLTRWTSTQVWKEHFDAWDVISEYANRCIWKVHQELRLGSSQTYSGIVAALITQGALPLDAIKANSMELTAGSVDTTAIPLVMTLFELARNPDVQQALRQETLAAEASIAANPQKAMSDLPLLRAALKETLRLYPVGGFLERILNSDLVLQNYHVPAGTLVLLYLYSMGRNPAVFPRPERYMPQRWLERKRSFQHLAFGFGVRQCLGRRLAEVEMLLLLHHMLKTFQVETLRQEDVQMAYRFVLMPSSSPVLTFRPIS
uniprot
proteins
ec_number
1.14.15.4
uniprot
proteins
ec_number
1.14.15.5
uniprot
proteins
function
FUNCTION: A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid responsible for salt and water homeostasis (PubMed:1562515, PubMed:1765101, PubMed:2350348, PubMed:2738055). Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that do not leave the enzyme active site during the consecutive hydroxylation reactions), and end with the formation of aldosterone (PubMed:1765101, PubMed:2350348, PubMed:2738055, PubMed:8333830). Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension (By similarity) (PubMed:1562515). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Besides its role in the biosynthesis of mineralocorticoids and glucocorticoids, it can also participate in the androgen metabolic pathway, converting testosterone (17beta-hydroxy-4-androsten-3-one) to 11beta,17beta-dihydroxyandrost-4-ene-3-one (By similarity). Can also use progesterone as substrate (By similarity). {ECO:0000250|UniProtKB:P19099, ECO:0000269|PubMed:1562515, ECO:0000269|PubMed:1765101, ECO:0000269|PubMed:2350348, ECO:0000269|PubMed:2738055, ECO:0000269|PubMed:8333830}.
uniprot
proteins
mass_dalton
58241
uniprot
proteins
pathway_participation
PATHWAY: Steroid biosynthesis. {ECO:0000305|PubMed:1765101}.
uniprot
proteins
protein_family
Cytochrome P450 family
uniprot
proteins
pubmed
1562515
uniprot
proteins
pubmed
1765101
uniprot
proteins
pubmed
2350348
uniprot
proteins
pubmed
2738055
uniprot
proteins
pubmed
8333830
uniprot
proteins
pubmed
8468320
uniprot
proteins
sequence_length
510
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250|UniProtKB:P14137}; Peripheral membrane protein {ECO:0000250|UniProtKB:P14137}.
uniprot
proteins