Enpp3
entrez
209558
canonical
uniprot
Q6DYE8
canonical
ensembl
ENSMUSG00000019989
resolver
ensp
ENSMUSP00000020169
resolver
enst
ENSMUST00000020169
resolver
genesymbol
Enpp3
resolver
uniprot
E9QMU8
resolver
uniprot_entry
ENPP3_MOUSE
resolver
uniprot_sec
E9QMU8
resolver
amino_acid_sequence
MDSRLALATEEPIKKDSLKKYKILCVVLLALLVIVSLGLGLGLGLRKPEEQGSCRKKCFDSSHRGLEGCRCDSGCTGRGDCCWDFEDTCVKSTQIWTCNLFRCGENRLETALCSCADDCLQRKDCCADYKTVCQGESPWVTEACASSQEPQCPPGFDLPPVILFSMDGFRAEYLQTWSTLLPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVHLNKNFSLSSVEKSNPAWWSGQPIWLTAMYQGLKAACYYWPGSDVAVNGSFPTIYRNYSNSVPYERRITTLLQWLDLPKADRPSFYTIYVEEPDSAGHSSGPVSAGVIKALQSVDNAFGMLMEGLKQRNLHNCVNIIVLADHGMDQTSCDRVEYMTDYFPKINFYMYQGPAPRIRTRNIPQDFFTFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKAHLMVDRQWLAFRSKGSSNCGGGTHGYNNEFKSMEAIFLAHGPSFIEKTVIEPFENIEVYNLLCDLLHIEPAPNNGTHGSLNHLLKTPFYKPSHAGELSTPADCGFTTPLPTDPLDCSCPALQNTPGLEEQANQRLNLSEGEVAATVKANLPFGRPRVMQKNGDHCLLYHRDYISGYGKAMKMPMWSSYTVLKPGDTSSLPPTVPDCLRADVRVAPSESQKCSFYLADKNITHGFLYPAIKGTNESRYDALITSNLVPMYKEFKKMWDYFHEVLLIKYAIERNGLNVVSGPIFDYNYDGHFDAPDEITQYVAGTDVPIPTHYFVVLTSCKDQTHTPDSCPGWLDVLPFIVPHRPTNIESCSENKTEDLWVEERFQAHAARVRDVELLTGLDFYQEKAQPVSQILQLKTYLPTFETII
uniprot
proteins
ec_number
3.1.4.1
uniprot
proteins
ec_number
3.6.1.-
uniprot
proteins
ec_number
3.6.1.9
uniprot
proteins
function
FUNCTION: Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (By similarity). Also hydrolyzes extracellular 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production, and is therefore involved in the regulation of innate immune response (PubMed:38749434). 2',3'-cGAMP appears to be further processed to GMP, AMP and inorganic phosphate (PubMed:38749434). Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner (PubMed:25692702). Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (PubMed:28225814). Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation (PubMed:23960081). Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well. In addition, displays an alkaline phosphodiesterase activity in vitro (By similarity). {ECO:0000250|UniProtKB:O14638, ECO:0000269|PubMed:23960081, ECO:0000269|PubMed:25692702, ECO:0000269|PubMed:28225814, ECO:0000269|PubMed:38749434}.
uniprot
proteins
mass_dalton
98662
uniprot
proteins
mutagenesis
MUTAGEN 205; /note="T->A: Loss of phosphodiesterase I activity. Loss of UDP-N-acetyl-alpha-D-glucosamine activity."; /evidence="ECO:0000269|PubMed:23960081"; MUTAGEN 329; /note="H->A: Loss of hydrolase activity toward 2',3'-cGAMP. Does not affect hydrolase activity toward ATP. Does not affect hydrolase activity toward UDP-N-acetyl-alpha-D-glucosamine."; /evidence="ECO:0000269|PubMed:38749434
uniprot
proteins
post_translational_modification
PTM: N-glycosylated. N-glycosylation is necessary for normal transport to the cell membrane, but is not the apical targeting signal. {ECO:0000250|UniProtKB:P97675}.
uniprot
proteins
pubmed
19349973
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
23960081
uniprot
proteins
pubmed
25692702
uniprot
proteins
pubmed
28225814
uniprot
proteins
pubmed
38749434
uniprot
proteins
sequence_length
874
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:25692702}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:O14638}. Apical cell membrane {ECO:0000250|UniProtKB:O14638}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:O14638}. Secreted {ECO:0000250|UniProtKB:O14638}. Note=Detected at the cell surface of basophils (PubMed:25692702). Detected at the apical plasma membrane of bile duct cells. Located to the apical surface in intestinal and kidney epithelial cells. Secreted in serum, and in lumen of epithelial cells (By similarity). {ECO:0000250|UniProtKB:O14638, ECO:0000269|PubMed:25692702}.
uniprot
proteins
transmembrane_region
TRANSMEM 12..30; /note="Helical; Signal-anchor for type II membrane protein"; /evidence="ECO:0000255
uniprot
proteins