SLC22A2
entrez
6582
canonical
uniprot
O15244
canonical
ensembl
ENSG00000112499
resolver
ensp
ENSP00000355920
resolver
genesymbol
SLC22A2
resolver
genesymbol_syn
OCT2
resolver
hgnc
HGNC:10966
resolver
uniprot
Q5T7Q6
resolver
uniprot
Q6PIQ8
resolver
uniprot
Q8NG62
resolver
uniprot
Q9NQB9
resolver
uniprot_entry
S22A2_HUMAN
resolver
uniprot_sec
Q5T7Q6
resolver
uniprot_sec
Q6PIQ8
resolver
uniprot_sec
Q8NG62
resolver
uniprot_sec
Q9NQB9
resolver
activity_regulation
ACTIVITY REGULATION: Tyrosine phosphorylation of the transporter leads to activation of the transport activity (PubMed:26979622). TEA uptake is activated by tyrosine phosphorylation (PubMed:26979622). Inhibited by cGMP, most likely through a cGMP-binding protein that interacts with OCT2 (PubMed:12395288). {ECO:0000269|PubMed:12395288, ECO:0000269|PubMed:26979622}.
uniprot
proteins
amino_acid_sequence
MPTTVDDVLEHGGEFHFFQKQMFFLLALLSATFAPIYVGIVFLGFTPDHRCRSPGVAELSLRCGWSPAEELNYTVPGPGPAGEASPRQCRRYEVDWNQSTFDCVDPLASLDTNRSRLPLGPCRDGWVYETPGSSIVTEFNLVCANSWMLDLFQSSVNVGFFIGSMSIGYIADRFGRKLCLLTTVLINAAAGVLMAISPTYTWMLIFRLIQGLVSKAGWLIGYILITEFVGRRYRRTVGIFYQVAYTVGLLVLAGVAYALPHWRWLQFTVSLPNFFFLLYYWCIPESPRWLISQNKNAEAMRIIKHIAKKNGKSLPASLQRLRLEEETGKKLNPSFLDLVRTPQIRKHTMILMYNWFTSSVLYQGLIMHMGLAGDNIYLDFFYSALVEFPAAFMIILTIDRIGRRYPWAASNMVAGAACLASVFIPGDLQWLKIIISCLGRMGITMAYEIVCLVNAELYPTFIRNLGVHICSSMCDIGGIITPFLVYRLTNIWLELPLMVFGVLGLVAGGLVLLLPETKGKALPETIEEAENMQRPRKNKEKMIYLQVQKLDIPLN
uniprot
proteins
function
FUNCTION: Electrogenic voltage-dependent uniporter that mediates the transport of a variety of organic cations such as endogenous bioactive amines, cationic drugs and xenobiotics (PubMed:9260930, PubMed:9687576). Functions as a Na(+)-independent, bidirectional uniporter (PubMed:21128598, PubMed:9687576). Cation cellular uptake or release is driven by the electrochemical potential, i.e. membrane potential and concentration gradient (PubMed:15212162, PubMed:9260930, PubMed:9687576). However, may also engage electroneutral cation exchange when saturating concentrations of cation substrates are reached (By similarity). Predominantly expressed at the basolateral membrane of hepatocytes and proximal tubules and involved in the uptake and disposition of cationic compounds by hepatic and renal clearance from the blood flow (PubMed:15783073). Implicated in monoamine neurotransmitters uptake such as histamine, dopamine, adrenaline/epinephrine, noradrenaline/norepinephrine, serotonin and tyramine, thereby supporting a physiological role in the central nervous system by regulating interstitial concentrations of neurotransmitters (PubMed:16581093, PubMed:17460754, PubMed:9687576). Also capable of transporting dopaminergic neuromodulators cyclo(his-pro), salsolinol and N-methyl-salsolinol, thereby involved in the maintenance of dopaminergic cell integrity in the central nervous system (PubMed:17460754). Mediates the bidirectional transport of acetylcholine (ACh) at the apical membrane of ciliated cell in airway epithelium, thereby playing a role in luminal release of ACh from bronchial epithelium (PubMed:15817714). Also transports guanidine and endogenous monoamines such as vitamin B1/thiamine, creatinine and N-1-methylnicotinamide (NMN) (PubMed:12089365, PubMed:15212162, PubMed:17072098, PubMed:24961373, PubMed:9260930). Mediates the uptake and efflux of choline (PubMed:9260930). Mediates the bidirectional transport of polyamine agmatine and the uptake of polyamines putrescine and spermidine (PubMed:12538837, PubMed:21128598). Able to transport non-amine endogenous compounds such as prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha) (PubMed:11907186). Also involved in the uptake of xenobiotic 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP) (PubMed:12395288, PubMed:16394027). May contribute to regulate the transport of organic compounds in testis across the blood-testis-barrier (Probable). {ECO:0000250|UniProtKB:Q9R0W2, ECO:0000269|PubMed:11907186, ECO:0000269|PubMed:12089365, ECO:0000269|PubMed:12395288, ECO:0000269|PubMed:12538837, ECO:0000269|PubMed:15212162, ECO:0000269|PubMed:15783073, ECO:0000269|PubMed:15817714, ECO:0000269|PubMed:16394027, ECO:0000269|PubMed:16581093, ECO:0000269|PubMed:17072098, ECO:0000269|PubMed:17460754, ECO:0000269|PubMed:21128598, ECO:0000269|PubMed:24961373, ECO:0000269|PubMed:9260930, ECO:0000269|PubMed:9687576, ECO:0000305|PubMed:35307651}.; FUNCTION: [Isoform 2]: In contrast with isoform 1, not able to transport guanidine, creatinine, cimetidine and metformin. {ECO:0000269|PubMed:12089365, ECO:0000269|PubMed:15212162, ECO:0000269|PubMed:16272756}.
uniprot
proteins
mass_dalton
62581
uniprot
proteins
mutagenesis
MUTAGEN 73; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 92; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 128; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 169; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 241; /note="Y->F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-362 and F-377."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 257; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 279; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 280; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 284; /note="P->A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 286; /note="S->A: No change in TEA and metformin uptake. No change in tyrosine phosphorylation."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 287; /note="P->A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 362; /note="Y->F: Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-241. Strong decrease in TEA uptake; when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-377."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 377; /note="Y->F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-362."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 458; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622"; MUTAGEN 544; /note="Y->F: No change in TEA uptake."; /evidence="ECO:0000269|PubMed:26979622
uniprot
proteins
post_translational_modification
PTM: Tyrosine phosphorylated by tyrosine-protein kinase YES1. {ECO:0000269|PubMed:26979622}.
uniprot
proteins
protein_family
Major facilitator (TC 2.A.1) superfamily
uniprot
proteins
protein_family
Organic cation transporter (TC 2.A.1.19) family
uniprot
proteins
pubmed
10942111
uniprot
proteins
pubmed
11907186
uniprot
proteins
pubmed
11912245
uniprot
proteins
pubmed
12089365
uniprot
proteins
pubmed
12142729
uniprot
proteins
pubmed
12395288
uniprot
proteins
pubmed
12538837
uniprot
proteins
pubmed
14574404
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15212162
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15496291
uniprot
proteins
pubmed
15783073
uniprot
proteins
pubmed
15817714
uniprot
proteins
pubmed
16006492
uniprot
proteins
pubmed
16263091
uniprot
proteins
pubmed
16272756
uniprot
proteins
pubmed
16314463
uniprot
proteins
pubmed
16394027
uniprot
proteins
pubmed
16581093
uniprot
proteins
pubmed
16914559
uniprot
proteins
pubmed
16951202
uniprot
proteins
pubmed
17072098
uniprot
proteins
pubmed
17111267
uniprot
proteins
pubmed
17393420
uniprot
proteins
pubmed
17460754
uniprot
proteins
pubmed
21128598
uniprot
proteins
pubmed
24961373
uniprot
proteins
pubmed
26979622
uniprot
proteins
pubmed
35307651
uniprot
proteins
pubmed
40972734
uniprot
proteins
pubmed
9260930
uniprot
proteins
pubmed
9687576
uniprot
proteins
sequence_length
555
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Basolateral cell membrane {ECO:0000250|UniProtKB:Q9R0W2}; Multi-pass membrane protein {ECO:0000305}. Basal cell membrane {ECO:0000269|PubMed:35307651}; Multi-pass membrane protein {ECO:0000305}. Apical cell membrane {ECO:0000269|PubMed:15817714, ECO:0000269|PubMed:9260930}; Multi-pass membrane protein {ECO:0000305}. Note=Localized to the basal membrane of Sertoli cells (PubMed:35307651). Localized to the basolateral membrane of proximal tubule (PubMed:11912245). Localized to the luminal/apical membrane of distal tubule (PubMed:9260930). Localized to the luminal/apical membrane of ciliated epithelial cells in bronchi (PubMed:15817714). {ECO:0000269|PubMed:11912245, ECO:0000269|PubMed:15817714, ECO:0000269|PubMed:35307651, ECO:0000269|PubMed:9260930}.
uniprot
proteins
transmembrane_region
TRANSMEM 23..43; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 151..171; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 178..198; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 209..229; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 239..259; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 264..284; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 349..369; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 376..396; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 415..435; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 442..462; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 465..485; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 495..515; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins