Alkbh5
entrez
268420
canonical
uniprot
Q3TSG4
canonical
ensembl
ENSMUSG00000042650
resolver
ensp
ENSMUSP00000049116
resolver
enst
ENSMUST00000044250
resolver
genesymbol
Alkbh5
resolver
genesymbol_syn
Abh5
resolver
genesymbol_syn
Ofoxd
resolver
uniprot
Q80US0
resolver
uniprot
Q8BKB9
resolver
uniprot
Q8BKC1
resolver
uniprot_entry
ALKB5_MOUSE
resolver
uniprot_sec
Q80US0
resolver
uniprot_sec
Q8BKB9
resolver
uniprot_sec
Q8BKC1
resolver
activity_regulation
ACTIVITY REGULATION: RNA demethylase activity is inhibited following sumoylation (By similarity). Inhibition is relieved following desumoylation (By similarity). Inhibited by histone demethylase inhibitor IOX1 (PubMed:36859428). {ECO:0000250|UniProtKB:Q6P6C2, ECO:0000269|PubMed:36859428}.
uniprot
proteins
amino_acid_sequence
MAAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPASGTTKRKYQEDSDPERSDYEEHQLQKEEEARKVKSGIRQIRLFSQDECSKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPDWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSTLPPSYASDRLSGNTRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCPEAASSPTRKVKMRRH
uniprot
proteins
ec_number
1.14.11.53
uniprot
proteins
function
FUNCTION: Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:29279410, PubMed:35939687, PubMed:36859428). Demethylates RNA by oxidative demethylation, which requires molecular oxygen, alpha-ketoglutarate and iron (By similarity). Demethylation of m6A mRNA affects mRNA processing, translation and export (PubMed:35939687, PubMed:36859428). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (By similarity). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (PubMed:23177736, PubMed:29279410). Involved in paraspeckle assembly, a nuclear membraneless organelle, by undergoing liquid-liquid phase separation (By similarity). Paraspeckle assembly is coupled with m6A demethylation of RNAs, such as NEAT1 non-coding RNA (By similarity). Also acts as a negative regulator of T-cell development: inhibits gamma-delta T-cell proliferation via demethylation of JAG1 and NOTCH2 transcripts (PubMed:35939687). Inhibits regulatory T-cell (Treg) recruitment by mediating demethylation and destabilization of CCL28 mRNAs (PubMed:36859428). {ECO:0000250|UniProtKB:Q6P6C2, ECO:0000269|PubMed:23177736, ECO:0000269|PubMed:29279410, ECO:0000269|PubMed:35939687, ECO:0000269|PubMed:36859428}.
uniprot
proteins
mass_dalton
44411
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Ser-88 and Ser-326 in response to reactive oxygen species (ROS), promoting sumoylation and inactivation. {ECO:0000250|UniProtKB:Q6P6C2}.; PTM: Acetylated by KAT8 at Lys-236, promoting interaction with PSPC1, thereby facilitating recognition of N(6)-methyladenosine (m6A) mRNA by ALKBH5. Deacetylated at Lys-236 by HDAC7. {ECO:0000250|UniProtKB:Q6P6C2}.; PTM: Sumoylated at Lys-87 and Lys-322 by PIAS4 following phosphorylation at Ser-88 and Ser-326 in response to reactive oxygen species (ROS), inhibiting the RNA demethylase activity. Desumoylated by SENP1; relieving RNA demethylase inhibition, leading to N(6)-methyladenosine-containing mRNAs demethylation. {ECO:0000250|UniProtKB:Q6P6C2}.; PTM: Ubiquitinated at Lys-58 via 'Lys-48'-linked polyubiquitin chain, leading to its degradation by the proteasome. Deubiquitinated at Lys-58 by USP9X, promoting its stabilizazion. {ECO:0000250|UniProtKB:Q6P6C2}.
uniprot
proteins
protein_family
AlkB family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17242355
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
23177736
uniprot
proteins
pubmed
29279410
uniprot
proteins
pubmed
35939687
uniprot
proteins
pubmed
36859428
uniprot
proteins
sequence_length
395
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:Q6P6C2}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q6P6C2}. Note=Promotes formation and localizes to paraspeckles, a nuclear membraneless organelle. {ECO:0000250|UniProtKB:Q6P6C2}.
uniprot
proteins