Cyp4a3
entrez
298423
canonical
uniprot
P20817
canonical
ensembl
ENSRNOG00000066878
resolver
ensp
ENSRNOP00000041025
resolver
enst
ENSRNOT00000051385
resolver
genesymbol
Cyp4a14
resolver
genesymbol
Cyp4a3
resolver
genesymbol_syn
Cyp4a-3
resolver
genesymbol_syn
Cyp4a3
resolver
uniprot
D3ZHZ6
resolver
uniprot_entry
CP4AE_RAT
resolver
uniprot_entry
D3ZHZ6_RAT
resolver
amino_acid_sequence
MGFSVFTPTRSLDGVSGFFQGAFLLSLFLVLFKAVQFYLRRQWLLKALEKFPSTPSHWLWGHDLKDREFQQVLTWVEKFPGACLQWLSGSKTRVLLYDPDYVKVVLGRSDPKASGIYQFLAPWIGYGLLLLNGKKWFQHRRMLTPAFHYGILKPYVKIMADSVNIMLDKWEKLDDQDHPLEIFHYVSLMTLDTVMKCAFSHQGSVQLDVNSRSYTKAVEDLNNLTFFRVRSAFYGNSIIYNMSSDGRLSRRACQIAHEHTDGVIKMRKAQLQNEEELQKARKKRHLDFLDILLFAKMEDGKSLSDEDLRAEVDTFMFEGHDTTASGISWVFYALATHPEHQERCREEVQSILGDGTSVTWDHLDQIPYTTMCIKEALRLYPPVPSVSRELSSPVTFPDGRSIPKGITTTILIYGLHHNPSYWPNPKVFDPSRFSPDSPRHSHAYLPFSGGARNCIGKQFAMNELKVAVALTLLRFELLPDPTRIPVPMARLVLKSKNGIHLRLKKLR
uniprot
proteins
ec_number
1.14.14.80
uniprot
proteins
function
FUNCTION: A cytochrome P450 monooxygenase that catalyzes omega and omega-1 hydroxylation of saturated fatty acids. Exhibits preferential omega versus omega-1 regioselectivity and (R) versus (S) stereoselectivity for hydroxylation of dodecanoic (lauric) acid. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). {ECO:0000269|PubMed:10869363}.
uniprot
proteins
mass_dalton
58232
uniprot
proteins
mutagenesis
MUTAGEN 63; /note="D->N: Has no significant effect on the catalytic activity toward lauric and myristic acids."; /evidence="ECO:0000269|PubMed:10869363"; MUTAGEN 91; /note="K->T: Impairs substrate binding."; /evidence="ECO:0000269|PubMed:10869363"; MUTAGEN 92; /note="T->A: Has no significant effect on the catalytic activity toward lauric and myristic acids."; MUTAGEN 113; /note="A->P: 70-fold increase of the binding constant for lauric acid associated with higher catalytic activity."; /evidence="ECO:0000269|PubMed:10869363"; MUTAGEN 114..116; /note="Missing: Higher kcat for hydroxylation of lauric acid. 2-fold increase of omega/(omega-1) hydroxylation ratio for lauric and myristic acids; when associated with S-119."; /evidence="ECO:0000269|PubMed:10869363"; MUTAGEN 119; /note="F->S: 7-fold increase of the binding constant for lauric acid associated with higher catalytic activity. 2-fold increase of omega/omega-1 hydroxylation ratio for lauric and myristic acids; when associated with S114_I116del."; /evidence="ECO:0000269|PubMed:10869363"; MUTAGEN 318; /note="E->A: Loss of covalent heme binding."; /evidence="ECO:0000269|PubMed:11821421"; MUTAGEN 318; /note="E->D: Significant reduction in covalent heme binding."; /evidence="ECO:0000269|PubMed:11821421"; MUTAGEN 318; /note="E->Q: Significant reduction in covalent heme binding."; /evidence="ECO:0000269|PubMed:11821421
uniprot
proteins
pathway_participation
PATHWAY: Lipid metabolism; fatty acid metabolism. {ECO:0000305|PubMed:10869363}.
uniprot
proteins
protein_family
Cytochrome P450 family
uniprot
proteins
pubmed
10869363
uniprot
proteins
pubmed
11139583
uniprot
proteins
pubmed
11821421
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
2766933
uniprot
proteins
pubmed
3365247
uniprot
proteins
sequence_length
507
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
uniprot
proteins