Slc12a5
entrez
171373
canonical
uniprot
Q63633
canonical
ensembl
ENSRNOG00000018111
resolver
ensembl
ENSRNOG00055004852
resolver
ensembl
ENSRNOG00060001320
resolver
ensembl
ENSRNOG00065013559
resolver
ensp
ENSRNOP00000024657
resolver
ensp
ENSRNOP00000093106
resolver
genesymbol
Slc12a5
resolver
genesymbol_syn
Kcc2
resolver
uniprot
A7Y821
resolver
uniprot_entry
S12A5_RAT
resolver
uniprot_sec
A7Y821
resolver
activity_regulation
ACTIVITY REGULATION: Inhibited following phosphorylation by OXSR1/OSR1 and STK39/SPAK: phosphorylation takes place downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4) in response to hyperosmotic stress and subsequent cell shrinkage. {ECO:0000269|PubMed:22544747}.
uniprot
proteins
amino_acid_sequence
MSRRFTVTSLPPAASAASADPESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSAAQKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS
uniprot
proteins
function
FUNCTION: Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis (PubMed:11551954). As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (PubMed:9930699). Involved in the regulation of dendritic spine formation and maturation (PubMed:22345354). {ECO:0000269|PubMed:11551954, ECO:0000269|PubMed:22345354, ECO:0000269|PubMed:9930699}.
uniprot
proteins
mass_dalton
126247
uniprot
proteins
mutagenesis
MUTAGEN 929; /note="T->A: Decreased phosphorylation by WNK kinases, leading to increased potassium-chloride cotransport activity; when associated with A-1030."; /evidence="ECO:0000269|PubMed:22544747"; MUTAGEN 929; /note="T->E: Mimics phosphorylation, leading to increased potassium-chloride cotransport activity; when associated with E-1030."; /evidence="ECO:0000269|PubMed:22544747"; MUTAGEN 1030; /note="T->A: Decreased phosphorylation by WNK kinases, leading to increased potassium-chloride cotransport activity; when associated with A-929."; /evidence="ECO:0000269|PubMed:22544747"; MUTAGEN 1030; /note="T->E: Mimics phosphorylation, leading to increased potassium-chloride cotransport activity; when associated with E-929."; /evidence="ECO:0000269|PubMed:22544747
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Thr-929 and Thr-1030 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. {ECO:0000269|PubMed:22544747}.
uniprot
proteins
protein_family
K/Cl co-transporter subfamily
uniprot
proteins
protein_family
SLC12A transporter family
uniprot
proteins
pubmed
11551954
uniprot
proteins
pubmed
17715129
uniprot
proteins
pubmed
22345354
uniprot
proteins
pubmed
22544747
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
8663311
uniprot
proteins
pubmed
9930699
uniprot
proteins
sequence_length
1139
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q91V14}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q91V14}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q91V14}. Note=Detected on dendrites, but not on axons of spinal cord neurons and at GPHN-positive inhibitory synapses. {ECO:0000250|UniProtKB:Q91V14}.
uniprot
proteins
transmembrane_region
TRANSMEM 123..143; /note="Discontinuously helical; Name=1"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 152..176; /note="Helical; Name=2"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 198..227; /note="Helical; Name=3"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 252..273; /note="Helical; Name=4"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 274..299; /note="Helical; Name=5"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 423..443; /note="Helical; Name=6"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 453..476; /note="Helical; Name=7"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 509..537; /note="Helical; Name=8"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 550..575; /note="Helical; Name=9"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 576..601; /note="Helical; Name=10"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 619..636; /note="Helical; Name=11"; /evidence="ECO:0000250|UniProtKB:Q91V14"; TRANSMEM 637..655; /note="Helical; Name=12"; /evidence="ECO:0000250|UniProtKB:Q91V14
uniprot
proteins