RNF144B
entrez
255488
canonical
uniprot
Q7Z419
canonical
ensembl
ENSG00000137393
resolver
ensp
ENSP00000259939
resolver
ensp
ENSP00000552852
resolver
genesymbol
RNF144B
resolver
genesymbol_syn
IBRDC2
resolver
genesymbol_syn
P53RFP
resolver
hgnc
HGNC:21578
resolver
uniprot
B3KUA8
resolver
uniprot
B4DZI2
resolver
uniprot
Q5TB85
resolver
uniprot
Q6P4Q0
resolver
uniprot
Q8N3R7
resolver
uniprot
Q9BX38
resolver
uniprot_entry
R144B_HUMAN
resolver
uniprot_sec
B3KUA8
resolver
uniprot_sec
B4DZI2
resolver
uniprot_sec
Q5TB85
resolver
uniprot_sec
Q6P4Q0
resolver
uniprot_sec
Q8N3R7
resolver
uniprot_sec
Q9BX38
resolver
amino_acid_sequence
MGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPYRTWCPVADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRALFGTDAEAPIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDKGPCRNKLGHSRASVMWNRTQVVGILVGLGIIALVTSPLLLLASPCIICCVCKSCRGKKKKHDPSTT
uniprot
proteins
ec_number
2.3.2.31
uniprot
proteins
function
FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. Plays a crucial role in maintaining the genomic stability by controlling the degradation of multiple proteins involved in mitotic progression and DNA damage (PubMed:38685100). Regulates epithelial homeostasis by mediating degradation of CDKN1A and isoform 2 of TP63 (PubMed:23128396). Plays a regulatory role in innate immunity by negatively regulating IRF3 activation and IFN-beta production. Mechanistically, inhibits TBK1 phosphorylation and 'Lys-63'-linked polyubiquitination independently of its E3 ligase activity (PubMed:31509299). Alternatively, promotes 'Lys-27' and 'Lys-33'-linked ubiquitination of IFIH1/MDA5, promoting selective autophagic degradation of IFIH1/MDA5 to inhibit antiviral response (PubMed:39285245). {ECO:0000269|PubMed:12853982, ECO:0000269|PubMed:20300062, ECO:0000269|PubMed:23128396, ECO:0000269|PubMed:31509299, ECO:0000269|PubMed:39285245}.
uniprot
proteins
mass_dalton
33697
uniprot
proteins
mutagenesis
MUTAGEN 48; /note="C->A: Strong loss of IFIH1/MDA5 ubiquitination."; /evidence="ECO:0000269|PubMed:39285245"; MUTAGEN 80; /note="C->A: Strong loss of IFIH1/MDA5 ubiquitination."; /evidence="ECO:0000269|PubMed:39285245"; MUTAGEN 206; /note="C->A: No loss in inhibitory effects on TBK1-induced IFN-beta promoter activity."; /evidence="ECO:0000269|PubMed:31509299"; MUTAGEN 219; /note="C->A: Strong loss of IFIH1/MDA5 ubiquitination."; /evidence="ECO:0000269|PubMed:39285245
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination.
uniprot
proteins
post_translational_modification
PTM: Auto-ubiquitinated.
uniprot
proteins
protein_family
RBR family
uniprot
proteins
protein_family
RNF144 subfamily
uniprot
proteins
pubmed
12853982
uniprot
proteins
pubmed
14574404
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16427630
uniprot
proteins
pubmed
17974005
uniprot
proteins
pubmed
20300062
uniprot
proteins
pubmed
23128396
uniprot
proteins
pubmed
31509299
uniprot
proteins
pubmed
38685100
uniprot
proteins
pubmed
39285245
uniprot
proteins
sequence_length
303
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000269|PubMed:20300062}; Single-pass membrane protein {ECO:0000269|PubMed:20300062}. Cytoplasm {ECO:0000269|PubMed:20300062, ECO:0000269|PubMed:39285245}. Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.
uniprot
proteins
transmembrane_region
TRANSMEM 258..278; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins