Mkln1
entrez
83536
canonical
uniprot
Q99PV3
canonical
ensembl
ENSRNOG00000054514
resolver
ensp
ENSRNOP00000077228
resolver
enst
ENSRNOT00000113146
resolver
genesymbol
Mkln1
resolver
genesymbol_syn
Msk
resolver
uniprot
A0A8I5Y5I8
resolver
uniprot_entry
A0A8I5Y5I8_RAT
resolver
uniprot_entry
MKLN1_RAT
resolver
amino_acid_sequence
MAAGGAVAAAPECRLLPYALHKWSSFSSTYLPENILVDKPNDQSSRWSSESNYPPQYLILKLERPAIVQNITFGKYEKTHVCNLKKFKVFGGMNEENMTELLSSGLKNDYNKETFTLKHKIDEQMFPCRFIKIVPLLSWGPSFNFSIWYVELSGIDDPDIVQPCLNWYSKYREQEAIRLCLKHFRQHNYTEAFESLQKKTKIALEHPMLTDMHDKLVLKGDFDACEELIEKAVNDGLFNQYISQQEYKPRWSQIIPKSTKGDGEDNRPGMRGGHQMVIDVQTETVYLFGGWDGTQDLADFWAYSVKENQWTCISRDTEKENGPSARSCHKMCIDIQRRQIYTLGRYLDSSVRNSKSLKSDFYRYDIDTNTWMLLSEDTAADGGPKLVFDHQMCMDSEKHMIYTFGGRILTCNGSVDDSRASEPQFSGLFAFNCQCQTWKLLREDSCNAGPEDIQSRIGHCMLFHSKNRCLYVFGGQRSKTYLNDFFSYDVDSDHVDIISDGTKKDSGMVPMTGFTQRATIDPELNEIHVLSGLSKDKEKREENVRNSFWIYDIVRNSWSCVYKNDQAAKENLSKSLQEEEPCPRFAHQLVYDELHKVHYLFGGNPGKSCSPKMRLDDFWSLKLCRPSKDYLLRHCKYLIRKHRFEEKAQMDPLSALKYLQNDLYITVDHSDPEETKEFQLLASALFKSGSDFTALGFSDVDHTYAQRTQLFDTLVNFFPDSMTPPKGNLVDLITL
uniprot
proteins
function
FUNCTION: Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (By similarity). Required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent GABRA1 degradation (PubMed:25579817). Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1 (By similarity). {ECO:0000250|UniProtKB:O89050, ECO:0000250|UniProtKB:Q9UL63, ECO:0000269|PubMed:25579817}.
uniprot
proteins
mass_dalton
84834
uniprot
proteins
mutagenesis
MUTAGEN 53; /note="Y->A: Reduced head to tail dimerization."; /evidence="ECO:0000269|PubMed:25579817"; MUTAGEN 143; /note="F->A: Strongly reduced head to tail dimerization."; /evidence="ECO:0000269|PubMed:25579817"; MUTAGEN 144; /note="N->R: Loss of head to tail dimerization. Causes loss of location in the cytosol and accumulation in the nucleus. No effect on GABA receptor internalization."; /evidence="ECO:0000269|PubMed:25579817"; MUTAGEN 180; /note="C->S: Reduced head to tail dimerization."; /evidence="ECO:0000269|PubMed:25579817"; MUTAGEN 184; /note="F->A: Reduced head to tail dimerization."; /evidence="ECO:0000269|PubMed:25579817"; MUTAGEN 184; /note="F->E: Strongly reduced head to tail dimerization and strongly reduced GABA receptor internalization; when associated with Q-196. Causes loss of location in the cytosol and accumulation in the nucleus; when associated with Q-196."; /evidence="ECO:0000269|PubMed:25579817"; MUTAGEN 185; /note="R->A: Reduced head to tail dimerization."; /evidence="ECO:0000269|PubMed:25579817"; MUTAGEN 196; /note="L->Q: Strongly reduced head to tail dimerization and strongly reduced GABA receptor internalization; when associated with E-184. Causes loss of location in the cytosol and accumulation in the nucleus; when associated with E-184."; /evidence="ECO:0000269|PubMed:25579817
uniprot
proteins
pubmed
11006128
uniprot
proteins
pubmed
21482357
uniprot
proteins
pubmed
25579817
uniprot
proteins
sequence_length
735
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11006128, ECO:0000269|PubMed:25579817}. Cell projection, ruffle {ECO:0000250|UniProtKB:O89050}. Cytoplasm, cell cortex {ECO:0000250|UniProtKB:O89050}. Synapse {ECO:0000250|UniProtKB:O89050}. Postsynapse {ECO:0000250|UniProtKB:O89050}. Note=Colocalizes with GABRA1 at synapses and in postsynaptic regions. Colocalizes with actin fibers in the cell cortex. {ECO:0000250|UniProtKB:O89050}.
uniprot
proteins