Qtrt1
entrez
64016
canonical
uniprot
Q4QR99
canonical
genesymbol
Qtrt1
resolver
uniprot
Q9JMA0
resolver
uniprot_entry
TGT_RAT
resolver
uniprot_sec
Q9JMA0
resolver
amino_acid_sequence
MAAVGSPGSLESAPRIMRLVAECSRSRARAGELRLPHGTVATPVFMPVGTQATMKGITAEQLDSLGCRICLGNTYHLGLRPGPELIQKAHGLHGFMNWPHNLLTDSGGFQMVSLISLSEVTEEGVRFRSPYDGEETLLSPERSVEIQNALGSDIIMQLDDVVSSTVTGPRVEEAMHRSVRWLDRCIAAHKRQDKQNLFAIIQGGLNADLRTTCLKEMTKRDVPGFAIGGLSGGESKEQFWKMVALSTSMLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVYPTRTARFGSALVPTGNLQLKKQQYAKDFSPINPECPCATCQTHSRAFLHTLLHSDNTAALHHLTVHNIAYQLQLLSAARSSILEQRFPDFVRNFMRTMYGDHSLCPAWAIEALASVGITLT
uniprot
proteins
ec_number
2.4.2.64
uniprot
proteins
function
FUNCTION: Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:6986171). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product. {ECO:0000255|HAMAP-Rule:MF_03218, ECO:0000269|PubMed:6986171}.
uniprot
proteins
mass_dalton
44187
uniprot
proteins
protein_family
Queuine tRNA-ribosyltransferase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
6986171
uniprot
proteins
sequence_length
403
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03218}. Mitochondrion outer membrane {ECO:0000255|HAMAP-Rule:MF_03218}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03218}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03218}. Note=Weakly associates with mitochondria, possibly via QTRT2. {ECO:0000255|HAMAP-Rule:MF_03218}.
uniprot
proteins