Nos2
entrez
18126
canonical
uniprot
P29477
canonical
ensembl
ENSMUSG00000020826
resolver
ensp
ENSMUSP00000018610
resolver
ensp
ENSMUSP00000150558
resolver
enst
ENSMUST00000018610
resolver
genesymbol
Nos2
resolver
genesymbol_syn
Inosl
resolver
uniprot
O70515
resolver
uniprot
O70516
resolver
uniprot
Q5SXT3
resolver
uniprot
Q6P6A0
resolver
uniprot
Q8R410
resolver
uniprot_entry
NOS2_MOUSE
resolver
uniprot_sec
O70515
resolver
uniprot_sec
O70516
resolver
uniprot_sec
Q5SXT3
resolver
uniprot_sec
Q6P6A0
resolver
uniprot_sec
Q8R410
resolver
activity_regulation
ACTIVITY REGULATION: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.
uniprot
proteins
amino_acid_sequence
MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLHVTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPWKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFNTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHEDIFGAVFSYGAKKGSALEEPKATRL
uniprot
proteins
ec_number
1.14.13.39
uniprot
proteins
function
FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed:16373578). As component of the iNOS-S100A8/9 transnitrosylase complex is involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (By similarity). {ECO:0000250|UniProtKB:P35228, ECO:0000250|UniProtKB:P79290, ECO:0000269|PubMed:16373578, ECO:0000269|PubMed:7503239}.
uniprot
proteins
mass_dalton
130575
uniprot
proteins
mutagenesis
MUTAGEN 22; /note="K->A: Reduced interaction with SPSB2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 23; /note="D->A: Significant loss of interaction with SPSB2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 25; /note="N->A,Q: Significant loss of interaction with SPSB2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 27; /note="N->A,Q: Loss of interaction with SPSB2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 28; /note="V->A: Reduced interaction with SPSB2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 30; /note="K->A: Reduced interaction with SPSB2."; /evidence="ECO:0000269|PubMed:20603330
uniprot
proteins
post_translational_modification
PTM: Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. {ECO:0000269|PubMed:20603330, ECO:0000269|PubMed:21199876}.
uniprot
proteins
protein_family
NOS family
uniprot
proteins
pubmed
10438970
uniprot
proteins
pubmed
10562538
uniprot
proteins
pubmed
10562539
uniprot
proteins
pubmed
10769116
uniprot
proteins
pubmed
11669619
uniprot
proteins
pubmed
12437348
uniprot
proteins
pubmed
12464241
uniprot
proteins
pubmed
1372907
uniprot
proteins
pubmed
1373522
uniprot
proteins
pubmed
1379716
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16373578
uniprot
proteins
pubmed
19144319
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
20603330
uniprot
proteins
pubmed
21199876
uniprot
proteins
pubmed
22073225
uniprot
proteins
pubmed
22081021
uniprot
proteins
pubmed
24970700
uniprot
proteins
pubmed
35978195
uniprot
proteins
pubmed
7503239
uniprot
proteins
pubmed
7544010
uniprot
proteins
pubmed
9334294
uniprot
proteins
pubmed
9516116
uniprot
proteins
sequence_length
1144
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P35228}. Note=Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. {ECO:0000250|UniProtKB:P35228}.
uniprot
proteins