NUDT5
entrez
11164
canonical
uniprot
Q9UKK9
canonical
ensembl
ENSG00000165609
resolver
ensp
ENSP00000419628
resolver
ensp
ENSP00000575451
resolver
enst
ENST00000491614
resolver
genesymbol
NUDT5
resolver
genesymbol_syn
NUDIX5
resolver
hgnc
HGNC:8052
resolver
uniprot
A8K516
resolver
uniprot
Q6IAG0
resolver
uniprot
Q9UH49
resolver
uniprot_entry
NUDT5_HUMAN
resolver
uniprot_sec
A8K516
resolver
uniprot_sec
Q6IAG0
resolver
uniprot_sec
Q9UH49
resolver
amino_acid_sequence
MESQEPTESSQNGKQYIISEELISEGKWVKLEKTTYMDPTGKTRTWESVKRTTRKEQTADGVAVIPVLQRTLHYECIVLVKQFRPPMGGYCIEFPAGLIDDGETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCTIHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAKPFEVPFLKF
uniprot
proteins
ec_number
2.7.7.96
uniprot
proteins
ec_number
3.6.1.13
uniprot
proteins
ec_number
3.6.1.58
uniprot
proteins
function
FUNCTION: Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate (PubMed:27257257). In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP (PubMed:10567213, PubMed:10722730, PubMed:17052728, PubMed:12717453, PubMed:19699693, PubMed:21389046, PubMed:38635563). Can also hydrolyze other nucleotide sugars with low activity (PubMed:12717453, PubMed:19699693, PubMed:21389046). In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). In vitro, it has decapping activity on 11-mer RNA capped with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by hydrolyzing the pyrophosphate bridge of the sugar cap (PubMed:38716860). Does not play a role in U8 snoRNA decapping (By similarity). Binds U8 snoRNA (By similarity). {ECO:0000250|UniProtKB:Q9JKX6, ECO:0000269|PubMed:10567213, ECO:0000269|PubMed:10722730, ECO:0000269|PubMed:12717453, ECO:0000269|PubMed:17052728, ECO:0000269|PubMed:19699693, ECO:0000269|PubMed:21389046, ECO:0000269|PubMed:27257257, ECO:0000269|PubMed:38635563, ECO:0000269|PubMed:38716860}.
uniprot
proteins
mass_dalton
24328
uniprot
proteins
mutagenesis
MUTAGEN 28; /note="W->A: Reduces affinity for substrate about 8-fold. Strongly reduced catalytic activity and strongly reduced affinity for substrate; when associated with A-46."; /evidence="ECO:0000269|PubMed:18462755"; MUTAGEN 45; /note="T->A: Impaired phosphorylation; generates ATP in the presence of diphosphate."; /evidence="ECO:0000269|PubMed:27257257"; MUTAGEN 45; /note="T->D: Phosphomimetic mutant; unable to generate ATP in the presence of diphosphate."; /evidence="ECO:0000269|PubMed:27257257"; MUTAGEN 46; /note="W->A: Reduces affinity for substrate about 6-fold. Strongly reduced catalytic activity and strongly reduced affinity for substrate; when associated with A-28."; /evidence="ECO:0000269|PubMed:18462755"; MUTAGEN 51; /note="R->Q: Reduces affinity for substrate about 15-fold and reduces catalytic rate about 17-fold."; /evidence="ECO:0000269|PubMed:18462755"; MUTAGEN 84; /note="R->Q: Reduces affinity for substrate about 5-fold and reduces catalytic rate 67-fold."; /evidence="ECO:0000269|PubMed:18462755"; MUTAGEN 98; /note="L->A: Reduces affinity for substrate about 6-fold."; /evidence="ECO:0000269|PubMed:18462755"; MUTAGEN 112; /note="E->Q: Catalytic inactive mutant for both ADP-sugar pyrophosphatase and nuclear ATP-synthesis activities. Reduces catalytic rate 6300-fold."; /evidence="ECO:0000269|PubMed:18462755, ECO:0000269|PubMed:27257257"; MUTAGEN 116; /note="E->Q: Reduces catalytic rate 2000-fold."; /evidence="ECO:0000269|PubMed:18462755"; MUTAGEN 166; /note="E->Q: Reduces catalytic rate 120-fold."; /evidence="ECO:0000269|PubMed:18462755
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Thr-45 is required for homodimer stability; dephosphorylation results in destabilization of the homodimer. Dephosphorylation at Thr-45 promotes the ATP-synthesis activity. {ECO:0000269|PubMed:27257257}.
uniprot
proteins
protein_family
Nudix hydrolase family
uniprot
proteins
pubmed
10567213
uniprot
proteins
pubmed
10722730
uniprot
proteins
pubmed
11042152
uniprot
proteins
pubmed
12717453
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15592455
uniprot
proteins
pubmed
17052728
uniprot
proteins
pubmed
18462755
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19608861
uniprot
proteins
pubmed
19699693
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21389046
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
21768126
uniprot
proteins
pubmed
22223895
uniprot
proteins
pubmed
22905912
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
27257257
uniprot
proteins
pubmed
28112733
uniprot
proteins
pubmed
38635563
uniprot
proteins
pubmed
38716860
uniprot
proteins
sequence_length
219
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:27257257}.
uniprot
proteins