Carm1
entrez
363026
canonical
uniprot
Q4AE70
canonical
ensembl
ENSRNOG00000031129
resolver
ensembl
ENSRNOG00055013317
resolver
ensembl
ENSRNOG00060030321
resolver
ensembl
ENSRNOG00065012588
resolver
ensp
ENSRNOP00000042739
resolver
ensp
ENSRNOP00000049428
resolver
genesymbol
Carm1
resolver
genesymbol_syn
Prmt4
resolver
uniprot
Q4AE68
resolver
uniprot
Q4AE69
resolver
uniprot
Q4AE71
resolver
uniprot_entry
CARM1_RAT
resolver
uniprot_sec
Q4AE68
resolver
uniprot_sec
Q4AE69
resolver
uniprot_sec
Q4AE71
resolver
activity_regulation
ACTIVITY REGULATION: Methylation of H3R17 (H3R17me) by CARM1 is stimulated by preacetylation of H3 'Lys-18' (H3K18ac) H3 'Lys-23' (H3K23ac) by EP300 and blocked by citrullination of H3 'Arg-17' (H3R17ci) by PADI4. {ECO:0000250|UniProtKB:Q9WVG6}.
uniprot
proteins
amino_acid_sequence
MAAAAATAVGPGAGSAGVAGPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDAAGIALYSHEDVCVFKCSVSRETECSRVGRQSFIITLGCNSVLIQFATPHDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGVAVAGMPTAYDLSSVIAGGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGNRVAGPWAGDLPPGLRTRSSYQWGPGRLRGHAGSSVPMTCPTGSSGAQGGGGSSSAHYAVNNQFTMGGPAISMASPMSIPTNTMHYGS
uniprot
proteins
ec_number
2.1.1.319
uniprot
proteins
function
FUNCTION: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability (PubMed:16508003). Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activation of transcription via chromatin remodeling (By similarity). During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription (By similarity). During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C (By similarity). During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B (By similarity). Acts as a coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue (By similarity). Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors (By similarity). Also seems to be involved in p53/TP53 transcriptional activation (By similarity). Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation (By similarity). Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs (PubMed:16508003). Acts as a transcriptional coactivator of ACACA/acetyl-CoA carboxylase by enriching H3R17 methylation at its promoter, thereby positively regulating fatty acid synthesis (By similarity). Independently of its methyltransferase activity, involved in replication fork progression: promotes PARP1 recruitment to replication forks, leading to poly-ADP-ribosylation of chromatin at replication forks and reduced fork speed (By similarity). {ECO:0000250|UniProtKB:Q86X55, ECO:0000250|UniProtKB:Q9WVG6, ECO:0000269|PubMed:16508003}.; FUNCTION: [Isoform 3]: Isoform 3 specifically affects pre-mRNA splicing (PubMed:15944154). This activity is independent from methyltransferase activity (PubMed:15944154). {ECO:0000269|PubMed:15944154}.
uniprot
proteins
mass_dalton
70341
uniprot
proteins
post_translational_modification
PTM: Auto-methylated on Arg-551. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity). {ECO:0000250|UniProtKB:Q9WVG6}.; PTM: Phosphorylation at Ser-217 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity. Phosphorylation at Ser-217 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-217 may promote location in the cytosol (By similarity). {ECO:0000250|UniProtKB:Q86X55, ECO:0000250|UniProtKB:Q9WVG6}.; PTM: Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO9 at Lys-228; leading to proteasomal degradation. {ECO:0000250|UniProtKB:Q86X55}.
uniprot
proteins
protein_family
Class I-like SAM-binding methyltransferase superfamily
uniprot
proteins
protein_family
Protein arginine N-methyltransferase family
uniprot
proteins
pubmed
15944154
uniprot
proteins
pubmed
16508003
uniprot
proteins
pubmed
17882262
uniprot
proteins
sequence_length
651
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16508003}. Cytoplasm {ECO:0000269|PubMed:16508003}. Chromosome {ECO:0000250|UniProtKB:Q86X55}. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane. Localizes to replication forks. {ECO:0000250|UniProtKB:Q86X55}.
uniprot
proteins