Ano1
entrez
101772
canonical
uniprot
Q8BHY3
canonical
ensembl
ENSMUSG00000031075
resolver
ensp
ENSMUSP00000112616
resolver
ensp
ENSMUSP00000113899
resolver
ensp
ENSMUSP00000159034
resolver
ensp
ENSMUSP00000257596
resolver
ensp
ENSMUSP00000257598
resolver
enst
ENSMUST00000118556
resolver
enst
ENSMUST00000121758
resolver
enst
ENSMUST00000238848
resolver
genesymbol
Ano1
resolver
genesymbol_syn
Tmem16a
resolver
uniprot
A0A0A0MQF2
resolver
uniprot
A0A5F8MPM2
resolver
uniprot
G8JL82
resolver
uniprot
Q6P5C6
resolver
uniprot
Q8BI26
resolver
uniprot
Q99JK1
resolver
uniprot_entry
A0A0A0MQF2_MOUSE
resolver
uniprot_entry
A0A5F8MPM2_MOUSE
resolver
uniprot_entry
ANO1_MOUSE
resolver
uniprot_entry
G8JL82_MOUSE
resolver
uniprot_sec
Q6P5C6
resolver
uniprot_sec
Q8BI26
resolver
uniprot_sec
Q99JK1
resolver
activity_regulation
ACTIVITY REGULATION: ATP and calmodulin are essential for its activation. Channel activity is inhibited by CFTR protein and by chloride inhibitors such as niflumic acid (NFA) and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) (By similarity). Activated by heat with activation seen at temperatures above 44 degrees Celsius (PubMed:22634729). Activated by BDNF in radial glial cells (PubMed:31147466). {ECO:0000250|UniProtKB:Q5XXA6, ECO:0000269|PubMed:22634729, ECO:0000269|PubMed:31147466}.
uniprot
proteins
amino_acid_sequence
MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL
uniprot
proteins
function
FUNCTION: Calcium-activated chloride channel (CaCC) (PubMed:18724360, PubMed:22002868, PubMed:22634729, PubMed:23840801, PubMed:24913262, PubMed:25779870, PubMed:28561733, PubMed:28963502, PubMed:29236684, PubMed:29236691, PubMed:31147466, PubMed:34089532, PubMed:34433575, PubMed:35135993). Plays a role in transepithelial anion transport and smooth muscle contraction (PubMed:28561733, PubMed:29236684, PubMed:29236691). Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression (By similarity). Required for basal and ATP-dependent mucus secretion in airways and intestine, probably by controlling exocytosis of mucus-filled granules by providing Ca(2+) to an apical signaling compartment (PubMed:30586313). Contributes to airway mucus expression induced by interleukins IL3 and IL8 and by the asthma-associated protein CLCA1 and is required for expression of mucin MUC5AC (By similarity). However, was shown in another study not to be required for MUC5AC expression (By similarity). Plays a role in the propagation of Ca(2+) waves in Kolliker's organ in the cochlea and contributes to the refinement of auditory brainstem circuitries prior to hearing onset (PubMed:35129434). In vomeronasal sensory neurons, modulates spontaneous firing patterns in the absence of stimuli as well as the firing pattern of pheromone-evoked activity (PubMed:34433575). Responsible for calcium-activated chloride channel activity in type I taste cells of the vallate papillae (PubMed:34089532). Acts as a heat sensor in nociceptive neurons (PubMed:22634729). In dorsal root ganglion neurons, plays a role in mediating non-histaminergic Mas-related G protein-coupled receptor (MRGPR)-dependent itching, acting as a downstream effector of MRGPRs (PubMed:35135993). In the developing brain, required for the Ca(2+)-dependent process extension of radial glial cells (PubMed:31147466). {ECO:0000250|UniProtKB:Q5XXA6, ECO:0000269|PubMed:18585372, ECO:0000269|PubMed:18724360, ECO:0000269|PubMed:22002868, ECO:0000269|PubMed:22075693, ECO:0000269|PubMed:22634729, ECO:0000269|PubMed:24913262, ECO:0000269|PubMed:25779870, ECO:0000269|PubMed:28561733, ECO:0000269|PubMed:28963502, ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691, ECO:0000269|PubMed:30586313, ECO:0000269|PubMed:31147466, ECO:0000269|PubMed:34089532, ECO:0000269|PubMed:34433575, ECO:0000269|PubMed:35129434, ECO:0000269|PubMed:35135993}.
uniprot
proteins
mass_dalton
110916
uniprot
proteins
mutagenesis
MUTAGEN 425; /note="E->A,K: Increased Ca(2+) sensitivity."; /evidence="ECO:0000269|PubMed:33378669"; MUTAGEN 428; /note="K->A,E: Decreased Ca(2+) sensitivity."; /evidence="ECO:0000269|PubMed:33378669"; MUTAGEN 515; /note="R->A: Decreased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:28561733"; MUTAGEN 535; /note="R->A: Decreased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:28561733"; MUTAGEN 546; /note="N->D: Decreased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 550; /note="I->A: Low constitutive channel activity. Decreased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; MUTAGEN 550; /note="I->K: Induces phospholipid scramblase activity."; /evidence="ECO:0000269|PubMed:39495104"; MUTAGEN 551; /note="I->K: Induces phospholipid scramblase activity."; /evidence="ECO:0000269|PubMed:39495104"; MUTAGEN 555; /note="E->K: Induces phospholipid scramblase activity."; /evidence="ECO:0000269|PubMed:39495104"; MUTAGEN 588; /note="K->A,Q: Decreased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:23021219, ECO:0000269|PubMed:28561733"; MUTAGEN 591; /note="N->A: Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 593; /note="Y->D: Decreased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 596; /note="I->A: Decreased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 599; /note="V->A: Increased threshold for activation by calcium. Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 599; /note="V->K,L,R: Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 621; /note="R->E: Reduced anion permeability and increased cation permeability."; /evidence="ECO:0000269|PubMed:18724360"; MUTAGEN 625; /note="C->A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current."; /evidence="ECO:0000269|PubMed:18724360"; MUTAGEN 630; /note="C->A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current."; /evidence="ECO:0000269|PubMed:18724360"; MUTAGEN 635; /note="C->A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current."; /evidence="ECO:0000269|PubMed:18724360"; MUTAGEN 639; /note="S->A: Decreased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 643; /note="L->A: Increased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 644; /note="G->A: Decreased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236691"; MUTAGEN 644; /note="G->P: Low constitutive channel activity. Decreased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236691"; MUTAGEN 645; /note="K->A: Decreased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:28561733"; MUTAGEN 645; /note="K->E: Reduced anion permeability and increased cation permeability."; /evidence="ECO:0000269|PubMed:18724360"; MUTAGEN 651; /note="N->A: Strongly increased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236691"; MUTAGEN 654; /note="E->A: Strongly increased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 656; /note="G->A,P: No effect on threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236691"; MUTAGEN 658; /note="P->A,G: Moderately increased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236691"; MUTAGEN 668; /note="K->E: Reduced anion permeability and increased cation permeability."; /evidence="ECO:0000269|PubMed:18724360"; MUTAGEN 709; /note="Q->A: Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 712; /note="F->A: Decreased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 716; /note="F->A: Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 730; /note="N->A: Strongly increased threshold for activation by calcium."; /evidence="ECO:0000269|PubMed:29236691"; MUTAGEN 784; /note="D->A: Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 791; /note="Y->A: Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 807; /note="H->A: Increased permeability to chloride ions."; /evidence="ECO:0000269|PubMed:29236684"; MUTAGEN 883; /note="D->A,K: Increased Ca(2+) sensitivity."; /evidence="ECO:0000269|PubMed:33378669"; MUTAGEN 888; /note="D->A,K,N: Decreased Ca(2+) sensitivity."; /evidence="ECO:0000269|PubMed:33378669
uniprot
proteins
protein_family
Anoctamin family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
18585372
uniprot
proteins
pubmed
18724360
uniprot
proteins
pubmed
18729231
uniprot
proteins
pubmed
20056604
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
22002868
uniprot
proteins
pubmed
22075693
uniprot
proteins
pubmed
22634729
uniprot
proteins
pubmed
23021219
uniprot
proteins
pubmed
23840801
uniprot
proteins
pubmed
24509911
uniprot
proteins
pubmed
24913262
uniprot
proteins
pubmed
25779870
uniprot
proteins
pubmed
28561733
uniprot
proteins
pubmed
28963502
uniprot
proteins
pubmed
29236684
uniprot
proteins
pubmed
29236691
uniprot
proteins
pubmed
29928889
uniprot
proteins
pubmed
30586313
uniprot
proteins
pubmed
30630631
uniprot
proteins
pubmed
31147466
uniprot
proteins
pubmed
31175367
uniprot
proteins
pubmed
33378669
uniprot
proteins
pubmed
34089532
uniprot
proteins
pubmed
34433575
uniprot
proteins
pubmed
35129434
uniprot
proteins
pubmed
35135993
uniprot
proteins
pubmed
39495104
uniprot
proteins
sequence_length
960
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000269|PubMed:18585372, ECO:0000269|PubMed:22075693, ECO:0000269|PubMed:24913262, ECO:0000269|PubMed:28561733, ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691}; Multi-pass membrane protein {ECO:0000269|PubMed:28561733, ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691}. Presynapse {ECO:0000269|PubMed:23840801}. Note=In differentiating airway epithelial cells, predominantly intracellular at day 0 but is apically localized by day 30 (By similarity). Expressed in the presynapse of retinal neurons (PubMed:23840801). {ECO:0000250|UniProtKB:Q5XXA6, ECO:0000269|PubMed:23840801}.
uniprot
proteins
transmembrane_region
TRANSMEM 334..354; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 407..427; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 494..514; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 543..563; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 582..602; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 632..652; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 700..720; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 721..741; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 759..779; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691"; TRANSMEM 867..887; /note="Helical"; /evidence="ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691
uniprot
proteins