P26434
uniprot
P26434
canonical
activity_regulation
ACTIVITY REGULATION: Activated upon hypertonic conditions. Insensitive to amiloride, a common inhibitor of sodium:proton exchangers. Inhibited by certain amiloride analogs such as 5-(N,N-hexamethylene)amiloride (HMA) and 5-(N,N-dimethyl)amiloride (DMA). Resistant to 5-(N-ethyl-N-isopropyl)amiloride (EIPA) and cimetidine inhibitors when compared to SLC9A1. Activated by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). {ECO:0000269|PubMed:7961960, ECO:0000269|PubMed:8944646, ECO:0000269|PubMed:9038815}.
uniprot
proteins
amino_acid_sequence
MGPAMLRAFSSWKWLLLLMVLTCLEASSYVNESSSPTGQQTPDARFAASSSDPDERISVFELDYDYVQIPYEVTLWILLASLAKIGFHLYHRLPHLMPESCLLIIVGALVGSIIFGTHHKSPPVMDSSIYFLYLLPPIVLESGYFMPTRPFFENIGSILWWAGLGALINAFGIGLSLYFICQIKAFGLGDINLLQNLLFGSLISAVDPVAVLAVFEEARVNEQLYMMIFGEALLNDGISVVLYNILIAFTKMHKFEDIEAVDILAGCARFVIVGCGGVFFGIIFGFISAFITRFTQNISAIEPLIVFMFSYLSYLAAETLYLSGILAITACAVTMKKYVEENVSQTSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFVCFTLAFCQIWRAISVFTLFYVSNQFRTFPFSIKDQLIIFYSGVRGAGSFSLAFLLPLTLFPRKKLFVTATLVVTYFTVFFQGITIGPLVRYLDVRKTNKKESINEELHIRLMDHLKAGIEDVCGQWSHYQVRDKFKKFDHRYLRKILIRRNQPKSSIVSLYKKLEMKQAIEMAETGLLSSVASPTPYQSERIQGIKRLSPEDVESMRDILTRNMYQVRQRTLSYNKYNLKPQTSEKQAKEILIRRQNTLRESLRKGQSLPWVKPAGTKNFRYLSFPYSNPQPARRGARAAESTGNPCCWLLHFLLCRAMVEKIWGPGGQETQPRLLCRNLN
uniprot
proteins
function
FUNCTION: Electroneutral antiporter that exchanges sodium for protons or ammonium ions at the basolateral membrane of epithelia to regulate cell volume and intracellular pH upon hypertonic conditions (PubMed:7961960, PubMed:8944646, PubMed:9038815). As part of transcellular ammonia transport in renal tubules, mediates basolateral ammonium extrusion in the medullary thick ascending limb, regulating the corticopapillary ammonium gradient and overall renal acid excretion (By similarity). Mediates sodium:proton exchange in gastric parietal cells secondary to cAMP-dependent acid secretion and hyperosmolarity. Possibly coupled to chloride:bicarbonate antiporter, enables loading of parietal cells with sodium and chloride ions to maintain cell volume and normal gastric acid secretion (By similarity). Functions as a sodium sensor in neurons of organum vasculosum of the lamina terminalis where it regulates water intake in response to increased sodium concentration in body fluids (By similarity). {ECO:0000250|UniProtKB:Q8BUE1, ECO:0000269|PubMed:7961960, ECO:0000269|PubMed:8944646, ECO:0000269|PubMed:9038815}.
uniprot
proteins
mass_dalton
81522
uniprot
proteins
post_translational_modification
PTM: May be phosphorylated.
uniprot
proteins
protein_family
Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
uniprot
proteins
pubmed
11553518
uniprot
proteins
pubmed
12576672
uniprot
proteins
pubmed
1577762
uniprot
proteins
pubmed
16158325
uniprot
proteins
pubmed
7961960
uniprot
proteins
pubmed
8944646
uniprot
proteins
pubmed
9038815
uniprot
proteins
pubmed
9374634
uniprot
proteins
pubmed
9610358
uniprot
proteins
pubmed
9691122
uniprot
proteins
pubmed
9755122
uniprot
proteins
sequence_length
717
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Basolateral cell membrane {ECO:0000269|PubMed:11553518, ECO:0000269|PubMed:9755122}; Multi-pass membrane protein {ECO:0000255}. Apical cell membrane {ECO:0000250|UniProtKB:Q8BUE1}; Multi-pass membrane protein {ECO:0000255}. Zymogen granule membrane {ECO:0000269|PubMed:9610358}; Multi-pass membrane protein {ECO:0000255}. Note=Enrichment at apical or basolateral membrane may be tissue-dependent. {ECO:0000305|PubMed:11553518, ECO:0000305|PubMed:9755122}.
uniprot
proteins
transmembrane_region
TRANSMEM 95..114; /note="Helical; Name=C/M3"; /evidence="ECO:0000255"; TRANSMEM 128..148; /note="Helical; Name=D/M4"; /evidence="ECO:0000255"; TRANSMEM 155..175; /note="Helical; Name=E/M5"; /evidence="ECO:0000255"; TRANSMEM 192..216; /note="Helical; Name=F/M5A"; /evidence="ECO:0000255"; TRANSMEM 226..247; /note="Helical; Name=G/M5B"; /evidence="ECO:0000255"; TRANSMEM 270..290; /note="Helical; Name=H/M6"; /evidence="ECO:0000255"; TRANSMEM 305..325; /note="Helical; Name=I/M7"; /evidence="ECO:0000255"; TRANSMEM 357..377; /note="Helical; Name=J/M8"; /evidence="ECO:0000255"; TRANSMEM 385..405; /note="Helical; Name=K/M9"; /evidence="ECO:0000255"; TRANSMEM 451..471; /note="Helical; Name=M/M10"; /evidence="ECO:0000255
uniprot
proteins