Rab3a
entrez
25531
canonical
uniprot
P63012
canonical
ensembl
ENSRNOG00000019433
resolver
ensp
ENSRNOP00000026392
resolver
genesymbol
Rab3a
resolver
uniprot
P05713
resolver
uniprot_entry
RAB3A_RAT
resolver
uniprot_sec
P05713
resolver
activity_regulation
ACTIVITY REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) including RAB3IL1 and MADD which promote the exchange of bound GDP for free GTP (PubMed:9020086). Regulated by GTPase activating proteins (GAPs) including RAB3GAP1 and TBC1D10B which increase the GTP hydrolysis activity (By similarity). Inhibited by GDP dissociation inhibitors (GDIs) which prevent Rab-GDP dissociation (Probable). Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity). {ECO:0000250|UniProtKB:P20336, ECO:0000250|UniProtKB:P63011, ECO:0000269|PubMed:9020086, ECO:0000305}.
uniprot
proteins
amino_acid_sequence
MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQGPQLTDQQAPPHQDCAC
uniprot
proteins
ec_number
3.6.5.2
uniprot
proteins
function
FUNCTION: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (By similarity). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). RAB3A plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (PubMed:21689256). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (PubMed:18407218). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (PubMed:17149709). Stimulates insulin secretion through interaction with RIMS2 and RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (By similarity). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (By similarity). Plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (PubMed:28057568). {ECO:0000250|UniProtKB:P20336, ECO:0000250|UniProtKB:P63011, ECO:0000269|PubMed:17149709, ECO:0000269|PubMed:18407218, ECO:0000269|PubMed:21689256, ECO:0000269|PubMed:28057568}.
uniprot
proteins
mass_dalton
24970
uniprot
proteins
mutagenesis
MUTAGEN 36; /note="T->N: No significant effect on interaction with RAB3IP."; /evidence="ECO:0000269|PubMed:7532276"; MUTAGEN 51; /note="F->L: Disrupts the interaction with RAB3IP."; /evidence="ECO:0000269|PubMed:7532276"; MUTAGEN 52; /note="V->A: No significant effect on interaction with RAB3IP."; /evidence="ECO:0000269|PubMed:7532276"; MUTAGEN 55; /note="V->E: Disrupts the interaction with RAB3IP."; /evidence="ECO:0000269|PubMed:7532276"; MUTAGEN 56; /note="G->D: Disrupts the interaction with RAB3IP."; /evidence="ECO:0000269|PubMed:7532276
uniprot
proteins
post_translational_modification
PTM: Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM and RAB GDP dissociation inhibitors GDI1 and GDI2. {ECO:0000250|UniProtKB:P20336}.
uniprot
proteins
protein_family
Rab family
uniprot
proteins
protein_family
Small GTPase superfamily
uniprot
proteins
pubmed
10025402
uniprot
proteins
pubmed
10196122
uniprot
proteins
pubmed
10748113
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
17149709
uniprot
proteins
pubmed
17311845
uniprot
proteins
pubmed
18407218
uniprot
proteins
pubmed
21689256
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
28057568
uniprot
proteins
pubmed
3317403
uniprot
proteins
pubmed
3344209
uniprot
proteins
pubmed
7532276
uniprot
proteins
pubmed
9020086
uniprot
proteins
sequence_length
220
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:17311845, ECO:0000269|PubMed:18407218}. Lysosome {ECO:0000250|UniProtKB:P20336}. Cytoplasmic vesicle, secretory vesicle {ECO:0000269|PubMed:17311845, ECO:0000269|PubMed:18407218}. Cell projection, axon {ECO:0000250|UniProtKB:P63011}. Cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Presynapse {ECO:0000250|UniProtKB:P63011}. Postsynapse {ECO:0000250|UniProtKB:P63011}. Note=Cycles between a vesicle-associated GTP-bound form and a cytosolic GDP-bound form. {ECO:0000269|PubMed:18407218}.
uniprot
proteins