P55016
uniprot
P55016
canonical
activity_regulation
ACTIVITY REGULATION: Activated following phosphorylation by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4). {ECO:0000250|UniProtKB:Q13621}.
uniprot
proteins
amino_acid_sequence
MSVNIPSNSVPSGASRFQVHVINEGHGSGAAMSDSTDPPHYEETSFGDEAQNRLKISFRPGNQECYENFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFRFANAVRVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIGIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVRACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS
uniprot
proteins
function
FUNCTION: Renal sodium, potassium and chloride non-electrogenic ion symporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (Probable) (PubMed:8021284). It can substitute NH4(+) for K(+), enabling NH4(+) apical transmembrane transport in the medullary thick ascending limb (MTAL). This function is crucial for maintaining ammonium homeostasis by the kidney, particularly during metabolic acidosis (By similarity). {ECO:0000250|UniProtKB:P55015, ECO:0000269|PubMed:8021284, ECO:0000305|PubMed:25967121}.
uniprot
proteins
mass_dalton
120597
uniprot
proteins
mutagenesis
MUTAGEN 96; /note="T->A: Loss of interaction with PPP3CB."; /evidence="ECO:0000269|PubMed:25967121"; MUTAGEN 96; /note="T->D: No effect on interaction with PPP3CB."; /evidence="ECO:0000269|PubMed:25967121"; MUTAGEN 101; /note="T->A: Loss of interaction with PPP3CB."; /evidence="ECO:0000269|PubMed:25967121"; MUTAGEN 101; /note="T->D: No effect on interaction with PPP3CB."; /evidence="ECO:0000269|PubMed:25967121"; MUTAGEN 114; /note="T->A: Loss of interaction with PPP3CB."; /evidence="ECO:0000269|PubMed:25967121"; MUTAGEN 114; /note="T->D: No effect on interaction with PPP3CB."; /evidence="ECO:0000269|PubMed:25967121
uniprot
proteins
post_translational_modification
PTM: Phosphorylated at Ser-87, Thr-96 and Thr-101 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity. {ECO:0000250|UniProtKB:Q13621}.
uniprot
proteins
protein_family
SLC12A transporter family
uniprot
proteins
pubmed
17341212
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
25967121
uniprot
proteins
pubmed
8021284
uniprot
proteins
sequence_length
1095
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000250|UniProtKB:P55014}; Multi-pass membrane protein {ECO:0000255}.
uniprot
proteins
transmembrane_region
TRANSMEM 174..194; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 198..218; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 256..276; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 299..319; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 324..344; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 376..396; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 414..434; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 547..567; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 568..588; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 606..626; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins