Fem1b
entrez
14155
canonical
uniprot
Q9Z2G0
canonical
ensembl
ENSMUSG00000032244
resolver
ensp
ENSMUSP00000034775
resolver
enst
ENSMUST00000034775
resolver
genesymbol
Fem1b
resolver
genesymbol_syn
F1aa
resolver
genesymbol_syn
Kiaa0396
resolver
uniprot
Q3TV57
resolver
uniprot
Q3ULQ3
resolver
uniprot
Q3V148
resolver
uniprot
Q80U13
resolver
uniprot
Q99NC9
resolver
uniprot
Q9QZL3
resolver
uniprot_entry
FEM1B_MOUSE
resolver
uniprot_sec
Q3TV57
resolver
uniprot_sec
Q3ULQ3
resolver
uniprot_sec
Q3V148
resolver
uniprot_sec
Q80U13
resolver
uniprot_sec
Q99NC9
resolver
uniprot_sec
Q9QZL3
resolver
activity_regulation
ACTIVITY REGULATION: Activity of the CRL2(FEM1B) complex toward FNIP1 is inhibited by BEX family proteins (BEX1, BEX2, BEX3 and/or BEX4) in absence of reductive stress (PubMed:34562363). Mechanistically, BEX proteins act as pseudosubstrate inhibitors that associate with FEM1B via zinc in absence of reductive stress, thereby preventing association between FEM1B and FNIP1 (PubMed:34562363). {ECO:0000269|PubMed:34562363}.
uniprot
proteins
amino_acid_sequence
MEGLAGYVYKAASEGKVLTLAALLLNRSESDIRYLLGYVSQQGGQRSTPLIIAARNGHAKVVRLLLEHYRVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVTNSTPLRAACFDGRLDIVKYLVENNANISIANKYDNTCLMIAAYKGHTDVVRYLLEQRADPNAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLSHADCDRRSRIEALELLGASFANDRENYDIMKTYHYLYLAMLERFQDGDNILEKEVLPPIHAYGNRTECRNPQELEAIRQDRDALHMEGLIVRERILGADNIDVSHPIIYRGAVYADNMEFEQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNEAVKAPDIECVLRCSVLEIEQSMNRVKNISDADVHSAMDNYECNLYTFLYLVCISTKTQCSEEDQCRINKQIYNLIHLDPRTREGFSLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIVQYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSLKCLAARAVRANDINYQDQIPRTLEEFVGFH
uniprot
proteins
function
FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (By similarity). The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (By similarity). The CRL2(FEM1B) complex specifically recognizes proteins ending with -Gly-Leu-Asp-Arg, such as CDK5R1, leading to their ubiquitination and degradation (By similarity). Also acts as a regulator of the reductive stress response by mediating ubiquitination of reduced FNIP1: in response to reductive stress, the CRL2(FEM1B) complex specifically recognizes a conserved Cys degron in FNIP1 when this degron is reduced, leading to FNIP1 degradation and subsequent activation of mitochondria to recalibrate reactive oxygen species (ROS) (PubMed:32941802, PubMed:34562363). Mechanistically, recognizes and binds reduced FNIP1 through two interface zinc ions, which act as a molecular glue that recruit reduced FNIP1 to FEM1B (PubMed:34562363). Promotes ubiquitination of GLI1, suppressing GLI1 transcriptional activator activity (By similarity). Promotes ubiquitination and degradation of ANKRD37 (PubMed:21723927). Promotes ubiquitination and degradation of SLBP (By similarity). Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis (By similarity). Also involved in glucose homeostasis in pancreatic islet (PubMed:16024793). May also act as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1 (By similarity). {ECO:0000250|UniProtKB:Q9UK73, ECO:0000269|PubMed:16024793, ECO:0000269|PubMed:21723927, ECO:0000269|PubMed:32941802, ECO:0000269|PubMed:34562363}.
uniprot
proteins
mass_dalton
70223
uniprot
proteins
mutagenesis
MUTAGEN 126; /note="R->A,Q: Abolished association with BEX family proteins (BEX1, BEX2, BEX3 and/or BEX4), leading to constitutive ubiquitination of FNIP1."; /evidence="ECO:0000269|PubMed:34562363"; MUTAGEN 186; /note="C->S: Abolished ability to promote ubiquitination of reduced FNIP1."; /evidence="ECO:0000269|PubMed:32941802"; MUTAGEN 597; /note="L->A: Abolished ability to promote ubiquitination of reduced FNIP1."; /evidence="ECO:0000269|PubMed:32941802
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:32941802, ECO:0000269|PubMed:34562363}.
uniprot
proteins
protein_family
Fem-1 family
uniprot
proteins
pubmed
10542291
uniprot
proteins
pubmed
12693553
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15601915
uniprot
proteins
pubmed
16024793
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
18816836
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21723927
uniprot
proteins
pubmed
32941802
uniprot
proteins
pubmed
34562363
uniprot
proteins
pubmed
9828124
uniprot
proteins
sequence_length
627
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15601915, ECO:0000269|PubMed:21723927}. Nucleus {ECO:0000250|UniProtKB:Q9UK73}. Note=In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. Associated with chromatin. {ECO:0000250|UniProtKB:Q9UK73}.
uniprot
proteins