Prag1
entrez
306506
canonical
uniprot
D3ZMK9
canonical
ensembl
ENSRNOG00000011407
resolver
ensp
ENSRNOP00000015166
resolver
enst
ENSRNOT00000015166
resolver
genesymbol
Prag1
resolver
genesymbol_syn
Pragmin
resolver
uniprot_entry
PRAG1_RAT
resolver
amino_acid_sequence
MSACSDFVEHIWKPGSCKNCFCLRSDHQLTAGHPKARASSLPAGARLPARPEICRLEDEGVNGLAYSKPTIAVKPTMMTSETADLWTEASLSAEVPKVNWRRTPGKLLLQKQEDGPIVYLGSFRGMQKAAGPLACTDSNSRCPPAYTMVGLHNLEARVDRNTALQPVNFQEEKAGREELPSAQESFRQKLAAFTGMTSSCLKGPRPCTSPQPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCRPESRDAVHNTEGSGRRRGDCSPICWEQGTCTRPTEEEKQALNFPRECCGQGSTANPPHLGPKKPSLNSEAASSSDGLSCGSSRSGANSPFAPHLENDYCSLVKEPTSVKQQDSGCHLVNSGKYVGQAVDLQPPALPREAVQPEPIYAESAKRKKAAPVPQRPEPKKEQVSSGQVWTGDTWSQKTPSGWSQEREGPNAAPQVATTITVIAAHPEEDHRTIYLSSPDSAVGVQWPRGSLNQDLHGSGEEPLVVQGLSSRESHPHNMTENSSKEKPAIPPKLSKSSPGGSPVSPAAPPLTDHSDGNTSGSSVGSQPSSRVPTNLTSSCQTNGVAAGDPAKCPPQANSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQELLSQSWGRELENGIADHSNSSTWHRLHPIDGASGQNGKTNSGMSKSASFAFEFPKDRGRLESFSPPPPPPKSRHLLKMNKSSSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLASDSRTCSDGGPSCEATHSPTISGKKLFAPVPFPSGSTEDVSPSGPAQPPPLPQKKIVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPLSYSLNSGNHPHHVFSSSEPLEKAFKGSVPWAPALGPANSKGGCGSPNLQGRAATSTSSSQLSVSSQASTGSSQLQLHSLLSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGLKTELRFDENSWSLFKLICNKPCCDSGDAIYYGATCSKDPDSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCGHFVASVPSSMLAFPDTSSKDPAPAAPSHTPAQEQDCVVVITREVPHQTASDFVRDSVASHRAEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCNPQSSPGPSANPSVPTTTSRCPSAAPAATTACQGGPGEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTLSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCPSEEVLCNTLHNWIDMKRALMMMKFAEKAVERRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL
uniprot
proteins
function
FUNCTION: Catalytically inactive protein kinase that acts as a scaffold protein (PubMed:29503074). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (PubMed:16481321). Promotes Src family kinase (SFK) signallig by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (PubMed:21873224, PubMed:27116701). Acts as a critical coactivator of Notch signaling (By similarity). {ECO:0000250|UniProtKB:Q571I4, ECO:0000269|PubMed:16481321, ECO:0000269|PubMed:21873224, ECO:0000269|PubMed:27116701, ECO:0000269|PubMed:29503074}.
uniprot
proteins
mass_dalton
147716
uniprot
proteins
mutagenesis
MUTAGEN 391; /note="Y->A: Does not affect cytoplasmic localization."; /evidence="ECO:0000269|PubMed:21873224"; MUTAGEN 391; /note="Y->F: Decreases the interaction with CSK."; /evidence="ECO:0000269|PubMed:21873224, ECO:0000269|PubMed:29503074"; MUTAGEN 939; /note="L->E: Does not affect homodimerization."; /evidence="ECO:0000269|PubMed:29503074"; MUTAGEN 978; /note="D->N: No ATP-binding activity."; /evidence="ECO:0000269|PubMed:29503074"; MUTAGEN 981; /note="Y->F: No ATP-binding activity."; /evidence="ECO:0000269|PubMed:29503074"; MUTAGEN 997; /note="K->A: Does not affect PRAG1-CSK complex formation. Does not affect PRAG1-induced protein tyrosine phosphorylation."; /evidence="ECO:0000269|PubMed:29503074"; MUTAGEN 1021; /note="Q->E: No ATP-binding activity."; /evidence="ECO:0000269|PubMed:29503074"; MUTAGEN 1329; /note="A->E: Strong reduction of homodimerization; Does not affect PRAG1-CSK complex formation. Reduces CSK activation."; /evidence="ECO:0000269|PubMed:29503074"; MUTAGEN 1349; /note="Y->E: Does not affect homodimerization."; /evidence="ECO:0000269|PubMed:29503074"; MUTAGEN 1362; /note="L->E: Does not affect homodimerization."; /evidence="ECO:0000269|PubMed:29503074
uniprot
proteins
post_translational_modification
PTM: Phosphorylated by CSK on Tyr-238, Tyr-343, and Tyr-391; Tyr-391 is a primary site of phosphorylation. {ECO:0000269|PubMed:27116701}.
uniprot
proteins
protein_family
Protein kinase superfamily
uniprot
proteins
pubmed
15057822
uniprot
proteins
pubmed
16481321
uniprot
proteins
pubmed
21873224
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
27116701
uniprot
proteins
pubmed
29503074
uniprot
proteins
sequence_length
1368
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21873224, ECO:0000269|PubMed:27116701}. Nucleus {ECO:0000250|UniProtKB:Q571I4}. Cell junction, focal adhesion {ECO:0000269|PubMed:27116701}. Note=Colocalized with NOTCH1 in the nucleus. {ECO:0000250|UniProtKB:Q571I4}.
uniprot
proteins