Phf5a
entrez
68479
canonical
uniprot
P83870
canonical
ensembl
ENSMUSG00000061360
resolver
ensp
ENSMUSP00000023117
resolver
enst
ENSMUST00000023117
resolver
genesymbol
Phf5a
resolver
uniprot
Q3TQX7
resolver
uniprot
Q9UH06
resolver
uniprot_entry
PHF5A_MOUSE
resolver
uniprot_sec
Q3TQX7
resolver
uniprot_sec
Q9UH06
resolver
amino_acid_sequence
MAKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR
uniprot
proteins
function
FUNCTION: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (By similarity). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (By similarity). Within the 17S U2 SnRNP complex, PHF5A is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (By similarity). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (By similarity). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (By similarity). Also involved in elongation by RNA polymerase II as part of the PAF1 complex (PAF1C) (PubMed:27749823). PAF1C is required for maintenance of embryonic stem cell (ESC) self-renewal and cellular reprogramming of stem cells (PubMed:27749823). Maintains pluripotency by recruiting and stabilizing PAF1C on pluripotency genes loci, and by regulating the expression of the pluripotency genes (PubMed:27749823). Regulates the deposition of elongation-associated histone modifications, including dimethylated histone H3 'Lys-79' (H3K79me2) and trimethylated histone H3 'Lys-36' (H3K36me3), on PAF1C targets, self-renewal and pluripotency genes (PubMed:27749823). Regulates RNA polymerase II promoter-proximal pause release of the PAF1C targets and self-renewal genes, and the levels of elongating ('Ser-2' phosphorylated) RNA polymerase II in their gene bodies (PubMed:27749823). Regulates muscle specification in adult stem cells by stabilizing PAF1C in chromatin to promote myogenic differentiation (PubMed:27749823). Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha (By similarity). {ECO:0000250|UniProtKB:P83871, ECO:0000250|UniProtKB:Q7RTV0, ECO:0000269|PubMed:27749823}.
uniprot
proteins
mass_dalton
12405
uniprot
proteins
protein_family
PHF5 family
uniprot
proteins
pubmed
12054543
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
18758164
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
23806337
uniprot
proteins
pubmed
27749823
uniprot
proteins
sequence_length
110
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12054543, ECO:0000269|PubMed:18758164, ECO:0000269|PubMed:27749823}. Nucleus speckle {ECO:0000269|PubMed:18758164}.
uniprot
proteins