EME2
entrez
197342
canonical
uniprot
A4GXA9
canonical
ensembl
ENSG00000197774
resolver
ensp
ENSP00000457353
resolver
genesymbol
EME2
resolver
hgnc
HGNC:27289
resolver
uniprot
Q8TEP2
resolver
uniprot
Q96RY3
resolver
uniprot_entry
EME2_HUMAN
resolver
uniprot_sec
Q8TEP2
resolver
uniprot_sec
Q96RY3
resolver
amino_acid_sequence
MARVGPGRAGVSCQGRGRGRGGSGQRRPPTWEISDSDAEDSAGSEAAARARDPAGERRAAAEALRLLRPEQVLKRLAVCVDTAILEDAGADVLMEALEALGCECRIEPQRPARSLRWTRASPDPCPRSLPPEVWAAGEQELLLLLEPEEFLQGVATLTQISGPTHWVPWISPETTARPHLAVIGLDAYLWSRQHVSRGTQQPESPKVAGAEVAVSWPEVEEALVLLQLWANLDVLLVASWQELSRHVCAVTKALAQYPLKQYRESQAFSFCTAGRWAAGEPVARDGAGLQAAWRRQIRQFSRVSPAVADAVVTAFPSPRLLQQALEACSTERERMGLLADLPVPPSEGGRPRRVGPDLSRRICLFLTTANPDLLLDLGS
uniprot
proteins
function
FUNCTION: Non-catalytic subunit of the structure-specific, heterodimeric DNA endonuclease MUS81-EME2 which is involved in the maintenance of genome stability. In the complex, EME2 is required for DNA cleavage, participating in DNA recognition and bending (PubMed:17289582, PubMed:24371268, PubMed:24813886, PubMed:35290797). MUS81-EME2 cleaves 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs (PubMed:24371268). MUS81-EME2 which is active during the replication of DNA is more specifically involved in replication fork processing (PubMed:17289582, PubMed:24813886). Replication forks frequently encounter obstacles to their passage, including DNA base lesions, DNA interstrand cross-links, difficult-to-replicate sequences, transcription bubbles, or tightly bound proteins. One mechanism for the restart of a stalled replication fork involves nucleolytic cleavage mediated by the MUS81-EME2 endonuclease. By acting upon the stalled fork, MUS81-EME2 generates a DNA double-strand break (DSB) that can be repaired by homologous recombination, leading to the restoration of an active fork (PubMed:24813886). MUS81-EME2 could also function in telomere maintenance (PubMed:24813886). {ECO:0000269|PubMed:17289582, ECO:0000269|PubMed:24371268, ECO:0000269|PubMed:24813886, ECO:0000269|PubMed:35290797}.
uniprot
proteins
mass_dalton
41178
uniprot
proteins
mutagenesis
MUTAGEN 294; /note="R->E: No effect on cleavage of 3 prime flaps, nHJs and 5 prime flaps; when associated with E-295."; /evidence="ECO:0000269|PubMed:35290797"; MUTAGEN 295; /note="R->E: No effect on cleavage of 3 prime flaps, nHJs and 5 prime flaps; when associated with E-294."; /evidence="ECO:0000269|PubMed:35290797"; MUTAGEN 352; /note="R->E: Decreased cleavage of 3 prime flaps, nHJs and 5 prime flaps; when associated with E-353."; /evidence="ECO:0000269|PubMed:35290797"; MUTAGEN 353; /note="R->E: Decreased cleavage of 3 prime flaps, nHJs and 5 prime flaps; when associated with E-353."; /evidence="ECO:0000269|PubMed:35290797"; MUTAGEN 360; /note="R->E: Decreased cleavage of 3 prime flaps, nHJs and 5 prime flaps; when associated with E-361."; /evidence="ECO:0000269|PubMed:35290797"; MUTAGEN 361; /note="R->E: Decreased cleavage of 3 prime flaps, nHJs and 5 prime flaps; when associated with E-360."; /evidence="ECO:0000269|PubMed:35290797
uniprot
proteins
protein_family
EME1/MMS4 family
uniprot
proteins
pubmed
11157797
uniprot
proteins
pubmed
12693554
uniprot
proteins
pubmed
17289582
uniprot
proteins
pubmed
24371268
uniprot
proteins
pubmed
24813886
uniprot
proteins
pubmed
35290797
uniprot
proteins
sequence_length
379
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:35290797}.
uniprot
proteins