CYP4F8
entrez
11283
canonical
uniprot
P98187
canonical
ensembl
ENSG00000186526
resolver
ensp
ENSP00000477567
resolver
enst
ENST00000612078
resolver
genesymbol
CYP4F8
resolver
hgnc
HGNC:2648
resolver
uniprot_entry
CP4F8_HUMAN
resolver
amino_acid_sequence
MSLLSLSWLGLRPVAASPWLLLLVVGASWLLARILAWTYAFYHNGRRLRCFPQPRKQNWFLGHLGLVTPTEEGLRVLTQLVATYPQGFVRWLGPITPIINLCHPDIVRSVINTSDAITDKDIVFYKTLKPWLGDGLLLSVGDKWRHHRRLLTPAFHFNILKPYIKIFSKSANIMHAKWQRLAMEGSTCLDVFEHISLMTLDSLQKCIFSFDSNCQEKPSEYITAIMELSALVVKRNNQFFRYKDFLYFLTPCGRRFHRACRLVHDFTDAVIQERRRTLTSQGVDDFLQAKAKSKTLDFIDVLLLSEDKNGKELSDEDIRAEADTFMFGGHDTTASGLSWVLYNLARHPEYQERCRQEVQELLKDREPKEIEWDDLAQLPFLTMCLKESLRLHPPIPTFARGCTQDVVLPDSRVIPKGNVCNINIFAIHHNPSVWPDPEVYDPFRFDPENAQKRSPMAFIPFSAGPRNCIGQKFAMAEMKVVLALTLLRFRILPDHREPRRTPEIVLRAEDGLWLRVEPLG
uniprot
proteins
ec_number
1.14.14.1
uniprot
proteins
function
FUNCTION: A cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives (oxylipins). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds, with preference for omega-1 and omega-2 positions (PubMed:10791960, PubMed:15789615, PubMed:16112640). Hydroxylates (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) predominantly at omega-2 position to form (18R)-hydroxyeicosatetraenoic acid (18R-HETE) (PubMed:10791960). Exhibits omega-1 hydroxylase activity toward prostaglandin (PG) H1, PGH2 and PGI2 (PubMed:10791960, PubMed:15789615). Catalyzes the epoxidation of double bonds of PUFAs, including docosahexaenoic and docosapentaenoic acids (PubMed:16112640). Shows little activity against PGD2, PGE1, PGE2, PGF2alpha, and leukotriene B4. {ECO:0000269|PubMed:10791960, ECO:0000269|PubMed:15789615, ECO:0000269|PubMed:16112640}.
uniprot
proteins
mass_dalton
59995
uniprot
proteins
mutagenesis
MUTAGEN 328; /note="G->E: No effect on U-44069 and U-51605 hydroxylation. 20:4n-6 hydroxylation shifted from C-18 to C-19."; /evidence="ECO:0000269|PubMed:16112640
uniprot
proteins
pathway_participation
PATHWAY: Lipid metabolism; fatty acid metabolism. {ECO:0000269|PubMed:10791960}.
uniprot
proteins
protein_family
Cytochrome P450 family
uniprot
proteins
pubmed
10405341
uniprot
proteins
pubmed
10791960
uniprot
proteins
pubmed
12464258
uniprot
proteins
pubmed
15789615
uniprot
proteins
pubmed
16112640
uniprot
proteins
sequence_length
520
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9HBI6}; Single-pass membrane protein {ECO:0000250|UniProtKB:Q9HBI6}. Microsome membrane {ECO:0000250|UniProtKB:Q9HBI6}; Single-pass membrane protein {ECO:0000250|UniProtKB:Q9HBI6}.
uniprot
proteins
transmembrane_region
TRANSMEM 15..37; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins