Alox12
entrez
287454
canonical
uniprot
F1LQ70
canonical
ensembl
ENSRNOG00000027037
resolver
ensp
ENSRNOP00000034009
resolver
enst
ENSRNOT00000033098
resolver
genesymbol
Alox12
resolver
uniprot_entry
LOX12_RAT
resolver
activity_regulation
ACTIVITY REGULATION: Activated by EGF. Arachidonic acid conversion is inhibited by (13S,14S)-epoxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate (13S,14S-epoxy-DHA) (By similarity). Arachidonate 12-lipoxygenase activity is decreased when PH decreases from 7.4 to 6 (By similarity). {ECO:0000250|UniProtKB:P18054, ECO:0000250|UniProtKB:P39655}.
uniprot
proteins
amino_acid_sequence
MGRYRVRVVTGAWLFSGSVNLVRLWLVGAHREARLELQLRPARGKEEEFDFDVAEDLGPLQFVKLHKEHTVVDDAWFCNLITVQGPGMSAEAVFPCYRWVQGEGILRLPEGTARLAGDNALDVFQKHREKELKERQQTYRWATWKQGLPQTIAADCKDDLPPNMRFHEEKRLDFEWTLKAGVLEMGLKRVYTLLRSWNRLEDFDQIFWGQKSALAEKVHRCWQEDELFGYQFLNGANPMLLRRSTSLPSRLVLPPGTEELQAQLEKELKDGCLFEADFILLDGIPANVIRGEQQYLAAPLVMLRMDPSGKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLAKIWVRNSDFQLQELQFHLLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRSRTQLISDGGIFDQVVSTGGGGHVQLLTRAVAQLTYCSLCPPDDLATRGLLRVPSALYAQDALQLWEVTARYVNGMVHLFYQSDDIVRGDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTSQHAAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETIMGSLPDVQKSCLQMTITWHLGRLQPDMVPLGHHKEKYFSDPRTKAVLSQFQADLENLEKEITARNQQLDLPYEYLKPSRIENSITI
uniprot
proteins
ec_number
1.13.11.-
uniprot
proteins
ec_number
1.13.11.31
uniprot
proteins
ec_number
1.13.11.33
uniprot
proteins
function
FUNCTION: Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:23382512). Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE. Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA. Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets (By similarity). An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:23382512). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic. Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (By similarity). {ECO:0000250|UniProtKB:P18054, ECO:0000250|UniProtKB:P39655, ECO:0000269|PubMed:23382512}.
uniprot
proteins
mass_dalton
75534
uniprot
proteins
pathway_participation
PATHWAY: Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis. {ECO:0000269|PubMed:23382512}.
uniprot
proteins
protein_family
Lipoxygenase family
uniprot
proteins
pubmed
15057822
uniprot
proteins
pubmed
15105833
uniprot
proteins
pubmed
22673903
uniprot
proteins
pubmed
23382512
uniprot
proteins
sequence_length
663
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Membrane {ECO:0000250}. Note=Membrane association is stimulated by EGF. {ECO:0000250}.
uniprot
proteins