Sphk1
entrez
20698
canonical
uniprot
Q8CI15
canonical
ensembl
ENSMUSG00000061878
resolver
ensp
ENSMUSP00000067865
resolver
ensp
ENSMUSP00000097775
resolver
ensp
ENSMUSP00000101994
resolver
ensp
ENSMUSP00000101995
resolver
ensp
ENSMUSP00000101996
resolver
ensp
ENSMUSP00000210174
resolver
ensp
ENSMUSP00000210175
resolver
genesymbol
Sphk1
resolver
genesymbol_syn
Sk1
resolver
uniprot
O88886
resolver
uniprot
Q3U2E3
resolver
uniprot
Q91ZN3
resolver
uniprot_entry
SPHK1_MOUSE
resolver
uniprot_sec
O88886
resolver
uniprot_sec
Q3U2E3
resolver
uniprot_sec
Q91ZN3
resolver
activity_regulation
ACTIVITY REGULATION: Acetyltransferase activity increases in presence of the kinase substrate, sphingosine (PubMed:29662056). In Purkinje cells, kinase activity on sphingosine increases in presence of VEGFA (PubMed:25417698). In neurons, kinase activity increases during the first 24h in presence of Amyloid-beta protein 42 to decrease after 96h (By similarity). {ECO:0000250|UniProtKB:Q91V26, ECO:0000269|PubMed:25417698, ECO:0000269|PubMed:29662056}.
uniprot
proteins
amino_acid_sequence
MEPVECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQVTNEDLLINCTLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQGQLAYLPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFVLVLVLLHTHLSSELFAAPMGRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRSQRGVFSVDGELMVCEAVQGQVHPNYLWMVCGSRDAPSGRDSRRGPPPEEP
uniprot
proteins
ec_number
2.3.1.-
uniprot
proteins
ec_number
2.7.1.91
uniprot
proteins
function
FUNCTION: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions (PubMed:17346996, PubMed:21084291, PubMed:25417698, PubMed:29662056, PubMed:33334894). Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed:9726979). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed:16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling (By similarity). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (By similarity). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed:27806293, PubMed:28049734). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed:25417698). {ECO:0000250|UniProtKB:Q9NYA1, ECO:0000269|PubMed:16118219, ECO:0000269|PubMed:21084291, ECO:0000269|PubMed:25417698, ECO:0000269|PubMed:27806293, ECO:0000269|PubMed:28049734, ECO:0000269|PubMed:33334894, ECO:0000269|PubMed:9726979, ECO:0000305|PubMed:29662056}.; FUNCTION: Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. {ECO:0000269|PubMed:29662056}.
uniprot
proteins
mass_dalton
42443
uniprot
proteins
mutagenesis
MUTAGEN 82; /note="G->D: No effect on recruitment to endocytic membrane. No effect on membrane fusion produced by sphingosine."; /evidence="ECO:0000269|PubMed:27806293
uniprot
proteins
pubmed
12077123
uniprot
proteins
pubmed
15196915
uniprot
proteins
pubmed
15459201
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16118219
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16314531
uniprot
proteins
pubmed
17346996
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21084291
uniprot
proteins
pubmed
24929359
uniprot
proteins
pubmed
25417698
uniprot
proteins
pubmed
27806293
uniprot
proteins
pubmed
28049734
uniprot
proteins
pubmed
29662056
uniprot
proteins
pubmed
33334894
uniprot
proteins
pubmed
9726979
uniprot
proteins
sequence_length
382
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16118219, ECO:0000269|PubMed:27806293, ECO:0000269|PubMed:29662056}. Endosome membrane {ECO:0000269|PubMed:24929359, ECO:0000269|PubMed:27806293}; Peripheral membrane protein {ECO:0000269|PubMed:24929359}. Nucleus {ECO:0000269|PubMed:29662056}. Cell membrane {ECO:0000250|UniProtKB:Q9NYA1}. Synapse {ECO:0000269|PubMed:24929359}. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner. Binds to membranes containing negatively charged lipids but not neutral lipids (By similarity). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (PubMed:27806293). {ECO:0000250|UniProtKB:Q9NYA1, ECO:0000269|PubMed:27806293}.
uniprot
proteins