P97562
uniprot
P97562
canonical
amino_acid_sequence
MGSPMHRVSLGDHWSWQVHPDIDSERHSPSFSVERLTNILDGGLPNTVLRRKVESIIQSDPVFNLKKLYFMTREELYEDAIQKRFHLEKLAWSLGWSEDGPERIYANRVLDGNVNLSLHGVAMNAIRSLGSDEQIAKWGQLCKNFQIITTYAQTELGHGTYLQGLETEATYDEARQELVIHSPTMTSTKWWPGDLGWSVTHAVVLAQLTCLGVRHGMHAFIVPIRSLEDHTPLPGITVGDIGPKMGLEHIDNGFLQLNHVRVPRENMLSRFAEVLPDGTYQRLGTPQSNYLGMLVTRVQLLCKGILPSLQKACIIATRYSVIRHQSRLRPSDPEAKILEYQTQQQKLLPQLAVSYAFHFTATSLSEFFHSSYSAILKRDFSLLPELHALSTGMKATFADFCAQGAEICRRACGGHGYSKLSGLPTLVARATASCTYEGENTVLYLQVARFLMKSYLQAQASPGATPQKPLPQSVMYIATQRPARCSAQTAADFRCPDVYTTAWAYVSTRLIRDAAHRTQTLMKSGVDQHDAWNQTTVIHLQAAKAHCYFITVKNFKEAVEKLDKEPEIQRVLQRLCDLYALHGVLTNSGDFLHDGFLSGAQVDMAREAFLDLLPLIRKDAILLTDAFDFSDHCLNSALGCYDGHVYERLFEWAQKYPANTQENPAYKKYIRPLMLGWRHKM
uniprot
proteins
ec_number
1.3.3.6
uniprot
proteins
function
FUNCTION: Involved in peroxisomal beta-oxidation of branched-chain fatty acids (BCFAs) and bile acids intermediates. Catalyzes the initial and rate-limiting dehydrogenation step that removes two hydrogen molecules and introduces a trans double bond between the alpha and beta carbons of the acyl CoA molecules while generating hydrogen peroxide as a by-product. Oxidizes its substrates in a stereospecific way and can only desaturate isomers carrying (2S)-methyl groups (Probable). In brown adipose tissue peroxisomes, mediates beta-oxidation of monomethyl branched-chain fatty acids with iso configuration (mmBCFAs) as part of a substrate synthesis and oxidation cycle fueled by FASN-dependent de novo mmBCFA synthesis. The mmBCFA catabolism is coupled to AMPK activation and mitochondrial ATP production as a thermogenic adaptation mechanism to maintain the body temperature in cold environment (By similarity). Metabolizes multi-methyl-branched fatty acyl-CoA esters such as (2S)-pristanoyl-CoA, displaying redundancy with ACOX3 (Probable). In the liver, mediates side-chain beta-oxidation of C27 bile acyl-CoA intermediates carrying a (25S)-methyl group to yield 24,25-trans unsaturated derivatives as part of peroxisomal bile acid synthesis pathway (Probable). Can oxidize straight-chain fatty acyl CoAs such as C10-CoA and C16-CoA with low efficiency (By similarity). {ECO:0000250|UniProtKB:Q99424, ECO:0000250|UniProtKB:Q9QXD1, ECO:0000305|PubMed:1400324}.
uniprot
proteins
mass_dalton
76799
uniprot
proteins
pathway_participation
PATHWAY: Lipid metabolism; peroxisomal fatty acid beta-oxidation. {ECO:0000250|UniProtKB:Q99424}.
uniprot
proteins
protein_family
Acyl-CoA oxidase family
uniprot
proteins
pubmed
1400324
uniprot
proteins
pubmed
16396499
uniprot
proteins
pubmed
8947475
uniprot
proteins
sequence_length
681
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:8947475}.
uniprot
proteins