PRIM2
entrez
5558
canonical
uniprot
P49643
canonical
ensembl
ENSG00000146143
resolver
ensp
ENSP00000483201
resolver
ensp
ENSP00000484105
resolver
ensp
ENSP00000485304
resolver
ensp
ENSP00000708789
resolver
ensp
ENSP00000773964
resolver
ensp
ENSP00000797706
resolver
genesymbol
PRIM2
resolver
genesymbol_syn
PRIM2A
resolver
hgnc
HGNC:9370
resolver
uniprot
Q53FJ8
resolver
uniprot
Q6P1Q7
resolver
uniprot
Q8WVL2
resolver
uniprot
Q9H413
resolver
uniprot_entry
PRI2_HUMAN
resolver
uniprot_sec
Q53FJ8
resolver
uniprot_sec
Q6P1Q7
resolver
uniprot_sec
Q8WVL2
resolver
uniprot_sec
Q9H413
resolver
amino_acid_sequence
MEFSGRKWRKLRLAGDQRNASYPHCLQFYLQPPSENISLIEFENLAIDRVKLLKSVENLGVSYVKGTEQYQSKLESELRKLKFSYRENLEDEYEPRRRDHISHFILRLAYCQSEELRRWFIQQEMDLLRFRFSILPKDKIQDFLKDSQLQFEAISDEEKTLREQEIVASSPSLSGLKLGFESIYKIPFADALDLFRGRKVYLEDGFAYVPLKDIVAIILNEFRAKLSKALALTARSLPAVQSDERLQPLLNHLSHSYTGQDYSTQGNVGKISLDQIDLLSTKSFPPCMRQLHKALRENHHLRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKGKMDPDKFDKGYSYNIRHSFGKEGKRTDYTPFSCLKIILSNPPSQGDYHGCPFRHSDPELLKQKLQSYKISPGGISQILDLVKGTHYQVACQKYFEMIHNVDDCGFSLNHPNQFFCESQRILNGGKDIKKEPIQPETPQPKPSVQKTKDASSALASLNSSLEMDMEGLEDYFSEDS
uniprot
proteins
function
FUNCTION: Regulatory subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis (PubMed:17893144, PubMed:25550159, PubMed:26975377, PubMed:9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerizing short RNA primers on both leading and lagging strands (PubMed:17893144). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity). In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit (PubMed:17893144, PubMed:25550159). Binds RNA:DNA duplex and coordinates the catalytic activities of PRIM1 and POLA2 during primase-to-polymerase switch. {ECO:0000250|UniProtKB:P09884, ECO:0000250|UniProtKB:P33610, ECO:0000269|PubMed:17893144, ECO:0000269|PubMed:25550159, ECO:0000269|PubMed:26975377, ECO:0000269|PubMed:9705292}.
uniprot
proteins
mass_dalton
58806
uniprot
proteins
mutagenesis
MUTAGEN 97; /note="R->A: Decreases primase affinity for POLA1 by 10-fold."; /evidence="ECO:0000269|PubMed:24043831"; MUTAGEN 104; /note="F->A: Decreases primase affinity for POLA1 by 40-fold."; /evidence="ECO:0000269|PubMed:24043831"; MUTAGEN 107; /note="R->A: Decreases primase affinity for POLA1 by 30-fold."; /evidence="ECO:0000269|PubMed:24043831"; MUTAGEN 108; /note="L->A: Decreases primase affinity for POLA1 by 40-fold."; /evidence="ECO:0000269|PubMed:24043831"; MUTAGEN 256..270; /note="Missing: Decreases RNA primer di-nucleotide formation about 5-fold. Does not affect the ratio between the di-nucleotide and its extension products."; /evidence="ECO:0000269|PubMed:25550159
uniprot
proteins
protein_family
Eukaryotic-type primase large subunit family
uniprot
proteins
pubmed
14574404
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
17893144
uniprot
proteins
pubmed
19369195
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24043831
uniprot
proteins
pubmed
25550159
uniprot
proteins
pubmed
26975377
uniprot
proteins
pubmed
8026492
uniprot
proteins
pubmed
9705292
uniprot
proteins
sequence_length
509
uniprot
proteins