RPA3
entrez
6119
canonical
uniprot
P35244
canonical
ensembl
ENSG00000106399
resolver
ensp
ENSP00000223129
resolver
ensp
ENSP00000379914
resolver
ensp
ENSP00000605666
resolver
enst
ENST00000223129
resolver
enst
ENST00000396682
resolver
enst
ENST00000935607
resolver
genesymbol
RPA3
resolver
genesymbol_syn
REPA3
resolver
genesymbol_syn
RPA14
resolver
hgnc
HGNC:10291
resolver
uniprot
A4D105
resolver
uniprot
Q549U6
resolver
uniprot_entry
A4D105_HUMAN
resolver
uniprot_entry
RFA3_HUMAN
resolver
uniprot_sec
Q549U6
resolver
amino_acid_sequence
MVDMMDLPRSRINAGMLAQFIDKPVCFVGRLEKIHPTGKMFILSDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEAVKIIHDFPQFYPLGIVQHD
uniprot
proteins
function
FUNCTION: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:17596542, PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Also plays a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). {ECO:0000269|PubMed:17596542, ECO:0000269|PubMed:19010961, ECO:0000269|PubMed:19116208, ECO:0000269|PubMed:19996105, ECO:0000269|PubMed:7697716, ECO:0000269|PubMed:9430682, ECO:0000269|PubMed:9765279, ECO:0000303|PubMed:24332808}.
uniprot
proteins
mass_dalton
13569
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination (PubMed:26474068). {ECO:0000269|PubMed:26474068}.
uniprot
proteins
protein_family
Replication factor A protein 3 family
uniprot
proteins
pubmed
11927569
uniprot
proteins
pubmed
12853948
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
17596542
uniprot
proteins
pubmed
19010961
uniprot
proteins
pubmed
19116208
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19996105
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22223895
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
24332808
uniprot
proteins
pubmed
25944712
uniprot
proteins
pubmed
26474068
uniprot
proteins
pubmed
7697716
uniprot
proteins
pubmed
7760808
uniprot
proteins
pubmed
8454588
uniprot
proteins
pubmed
9430682
uniprot
proteins
pubmed
9765279
uniprot
proteins
sequence_length
121
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17596542, ECO:0000269|PubMed:9430682}.
uniprot
proteins