Spsb2
entrez
14794
canonical
uniprot
O88838
canonical
ensembl
ENSMUSG00000038451
resolver
ensp
ENSMUSP00000041585
resolver
ensp
ENSMUSP00000060124
resolver
ensp
ENSMUSP00000118347
resolver
ensp
ENSMUSP00000206757
resolver
ensp
ENSMUSP00000206758
resolver
ensp
ENSMUSP00000206759
resolver
ensp
ENSMUSP00000206760
resolver
ensp
ENSMUSP00000206761
resolver
ensp
ENSMUSP00000206762
resolver
ensp
ENSMUSP00000360917
resolver
genesymbol
Spsb2
resolver
genesymbol_syn
Grcc9
resolver
genesymbol_syn
Ssb2
resolver
uniprot
Q8CBA5
resolver
uniprot
Q91Z15
resolver
uniprot_entry
SPSB2_MOUSE
resolver
uniprot_sec
Q8CBA5
resolver
uniprot_sec
Q91Z15
resolver
amino_acid_sequence
MGQTALARGSSSTPTSQALYSDFSPPEGLEELLSAPPPDLVAQRHHGWNPKDCSENIDVKEGGLCFERRPVAQSTDGVRGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQADHYAALLGSNSESWGWDIGRGKLYHQSKGLEAPQYPAGPQGEQLVVPERLLVVLDMEEGTLGYSIGGTYLGPAFRGLKGRTLYPSVSAVWGQCQVRIRYMGERRVEEPQSLLHLSRLCVRHALGDTRLGQISTLPLPPAMKRYLLYK
uniprot
proteins
function
FUNCTION: Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:20603330). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:20603330). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity). {ECO:0000250|UniProtKB:Q99619, ECO:0000269|PubMed:20603330}.
uniprot
proteins
mass_dalton
28938
uniprot
proteins
mutagenesis
MUTAGEN 100..101; /note="RG->AA: Loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 102..103; /note="TH->AA: Significant loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 118..119; /note="DH->AA: No loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 120; /note="Y->A: Loss of interaction with PARW. No effect on interaction with MET. Loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:16369487, ECO:0000269|PubMed:20561531, ECO:0000269|PubMed:20603330"; MUTAGEN 120; /note="Y->F: Loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 123..125; /note="LLG->AAA: Loss of interaction with MET and PARW. Loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:16369487, ECO:0000269|PubMed:20561531, ECO:0000269|PubMed:20603330"; MUTAGEN 126..128; /note="SNS->AAA: Loss of interaction with PAWR. Significant loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:20561531, ECO:0000269|PubMed:20603330"; MUTAGEN 141..143; /note="YHQ->AAA: Strongly reduced interaction with MET and PARW."; /evidence="ECO:0000269|PubMed:16369487"; MUTAGEN 151..152; /note="QY->AA: Loss of interaction with MET and PARW."; /evidence="ECO:0000269|PubMed:16369487"; MUTAGEN 160..161; /note="QL->AA: No loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:20603330"; MUTAGEN 188..189; /note="GP->AA: Strongly reduced interaction with MET. Loss of interaction with PARW."; /evidence="ECO:0000269|PubMed:16369487"; MUTAGEN 198; /note="T->A: No loss of interaction with PAWR."; /evidence="ECO:0000269|PubMed:20561531"; MUTAGEN 206; /note="V->A: Loss of interaction with PARW. No effect on interaction with MET. Loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:16369487, ECO:0000269|PubMed:20561531, ECO:0000269|PubMed:20603330"; MUTAGEN 207; /note="W->A: Significant loss of interaction with NOS2."; /evidence="ECO:0000269|PubMed:20603330
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination.
uniprot
proteins
protein_family
SPSB family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
16369487
uniprot
proteins
pubmed
20561531
uniprot
proteins
pubmed
20603330
uniprot
proteins
pubmed
9445485
uniprot
proteins
sequence_length
264
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q99619}. Note=Exhibits a diffuse cytosolic localization. {ECO:0000250|UniProtKB:Q99619}.
uniprot
proteins