Piwil1
entrez
57749
canonical
uniprot
Q9JMB7
canonical
ensembl
ENSMUSG00000029423
resolver
ensp
ENSMUSP00000083222
resolver
ensp
ENSMUSP00000344592
resolver
genesymbol
Piwil1
resolver
genesymbol_syn
Miwi
resolver
uniprot
A1L324
resolver
uniprot
A1L325
resolver
uniprot
Q6NXX0
resolver
uniprot_entry
PIWL1_MOUSE
resolver
uniprot_sec
A1L324
resolver
uniprot_sec
A1L325
resolver
uniprot_sec
Q6NXX0
resolver
amino_acid_sequence
MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL
uniprot
proteins
ec_number
3.1.26.-
uniprot
proteins
function
FUNCTION: Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:11578866, PubMed:21237665, PubMed:22121019). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:11578866, PubMed:21237665, PubMed:22121019). Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:11578866, PubMed:21237665, PubMed:22121019). Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias) (PubMed:24757166). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle (PubMed:22121019). Acts as an endoribonuclease that cleaves transposon messenger RNAs (PubMed:22121019). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (PubMed:16938833). Probable component of some RISC complex, which mediates RNA cleavage and translational silencing (PubMed:16938833). Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (PubMed:16787948). Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1 (PubMed:28552346). {ECO:0000269|PubMed:11578866, ECO:0000269|PubMed:16787948, ECO:0000269|PubMed:16938833, ECO:0000269|PubMed:21237665, ECO:0000269|PubMed:22121019, ECO:0000269|PubMed:24757166, ECO:0000269|PubMed:28552346}.
uniprot
proteins
mass_dalton
98574
uniprot
proteins
mutagenesis
MUTAGEN 218..221; /note="RRLL->ARLA: Does not affect piRNA-binding but abolishes ubiquitination by the APC/C. Causes male sterility."; /evidence="ECO:0000269|PubMed:23328397, ECO:0000269|PubMed:28552346"; MUTAGEN 330..335; /note="KSTFKK->ASTFAA: Abolishes ubiquitination by the APC/C."; /evidence="ECO:0000269|PubMed:23328397"; MUTAGEN 343; /note="F->A: Impairs binding to 2'-O-methylated 3'-end of piRNAs."; /evidence="ECO:0000269|PubMed:21237665"; MUTAGEN 346..347; /note="YY->AA: Abolishes piRNA-binding and ubiquitination by the APC/C."; /evidence="ECO:0000269|PubMed:23328397"; MUTAGEN 382; /note="M->A: Impairs binding to 2'-O-methylated 3'-end of piRNAs."; /evidence="ECO:0000269|PubMed:21237665"; MUTAGEN 633; /note="D->A: In DAH mutant; causes male infertility due to derepression of LINE1 retrotransposons transcripts."; /evidence="ECO:0000269|PubMed:22121019
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in late spermatids, leading to its degradation (PubMed:23328397). Ubiquitination only takes place following piRNA-binding in adult testis (PubMed:23328397). Ubiquitination and degradation in late spermatogenesis by APC/C is probably required to release RNF8 from the cytoplasm and promote histone to protamine exchange by RNF8 (PubMed:28552346). {ECO:0000269|PubMed:23328397, ECO:0000269|PubMed:28552346}.; PTM: Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. {ECO:0000269|PubMed:19377467, ECO:0000269|PubMed:19465913, ECO:0000269|PubMed:19584108, ECO:0000269|PubMed:19918066, ECO:0000269|PubMed:19926723}.
uniprot
proteins
protein_family
Argonaute family
uniprot
proteins
protein_family
Piwi subfamily
uniprot
proteins
pubmed
11578866
uniprot
proteins
pubmed
12062093
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16751776
uniprot
proteins
pubmed
16766680
uniprot
proteins
pubmed
16787948
uniprot
proteins
pubmed
16787967
uniprot
proteins
pubmed
16938833
uniprot
proteins
pubmed
19020299
uniprot
proteins
pubmed
19377467
uniprot
proteins
pubmed
19465913
uniprot
proteins
pubmed
19584108
uniprot
proteins
pubmed
19918066
uniprot
proteins
pubmed
19926723
uniprot
proteins
pubmed
20534472
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
21237665
uniprot
proteins
pubmed
22121019
uniprot
proteins
pubmed
22900038
uniprot
proteins
pubmed
23328397
uniprot
proteins
pubmed
23523368
uniprot
proteins
pubmed
23714778
uniprot
proteins
pubmed
24757166
uniprot
proteins
pubmed
28254886
uniprot
proteins
pubmed
28552346
uniprot
proteins
pubmed
39868537
uniprot
proteins
sequence_length
862
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11578866, ECO:0000269|PubMed:12062093, ECO:0000269|PubMed:16787948, ECO:0000269|PubMed:16938833, ECO:0000269|PubMed:19020299, ECO:0000269|PubMed:19584108}. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Also present in chromatoid body.
uniprot
proteins