Slc17a8
entrez
216227
canonical
uniprot
Q8BFU8
canonical
ensembl
ENSMUSG00000019935
resolver
ensp
ENSMUSP00000020102
resolver
ensp
ENSMUSP00000100932
resolver
enst
ENSMUST00000020102
resolver
enst
ENSMUST00000105295
resolver
genesymbol
Slc17a8
resolver
genesymbol_syn
Vglut3
resolver
uniprot
D3YTT3
resolver
uniprot
Q3UE85
resolver
uniprot_entry
D3YTT3_MOUSE
resolver
uniprot_entry
Q3UE85_MOUSE
resolver
uniprot_entry
VGLU3_MOUSE
resolver
activity_regulation
ACTIVITY REGULATION: The L-glutamate uniporter activity exhibits a biphasic dependence on chloride concentration. Chloride channel activity is allosterically activated by lumenal H(+) and Cl(-) leading to synaptic vesicles acidification. The L-glutamate transport activity is allosterically activated by lumenal H(+) and Cl(-), preventing non-vesicular L-glutamate release. {ECO:0000250|UniProtKB:Q7TSF2}.
uniprot
proteins
amino_acid_sequence
MPFKAFDTFKEKILKPGKEGVKNAVGDSLGILQRKIDGTNEEEDAIELNEEGRPVQTSRAHRPVCDCSCCGIPKRYICDCSCCGIPKRYIIAVMSGLGFCISFGIRCNLGVAIVEMVNNSTVYVDGKPEIQTAQFNWDPETVGLIHGSFFWGYIVTQIPGGFISNKFAASRVFGAAIFLTSTLNMFIPSAARVHYGCVMGVRILQGLVEGVTYPACHGMWSKWAPPLERSRLATTSFCGSYAGAVVAMPLAGVLVQYIGWASVFYIYGMFGIIWYMFWLLQAYECPAAHPTISNAERTYIETSIGEGANLASLSKFNTPWRRFFTSLPVYAIIVANFCRSWTFYLLLISQPAYFEEVFGFAISKVGLLSAVPHMVMTIVVPIGGQLADYLRSRKILTTTAVRKIMNCGGFGMEATLLLVVGFSHTKGVAISFLVLAVGFSGFAISGFNVNHLDIAPRYASILMGISNGVGTLSGMVCPLIVGAMTKHKTREEWQNVFLIAALVHYSGVIFYGVFASGEKQDWADPENLSEDKCGIIDQDELAEETELNHETFVSPRKKMSYGATTQNCEVQKTEWRQQRESAFDGEEPLSYQAEGDFSETS
uniprot
proteins
function
FUNCTION: Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, sodium and phosphate (PubMed:12384506, PubMed:18080752, PubMed:18215623). At the synaptic vesicle membrane, mainly functions as an uniporter that mediates the uptake of L-glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed:12384506, PubMed:18080752, PubMed:18215623). The L-glutamate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (PubMed:12384506). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (By similarity). The symporter activity is electrogenic (By similarity). Moreover, operates synergistically with SLC18A3/VACHT under a constant H(+) gradient, thereby allowing striatal vesicular acetylcholine uptake (PubMed:18278042). {ECO:0000250|UniProtKB:Q7TSF2, ECO:0000250|UniProtKB:Q8NDX2, ECO:0000269|PubMed:12384506, ECO:0000269|PubMed:18080752, ECO:0000269|PubMed:18215623, ECO:0000269|PubMed:18278042}.
uniprot
proteins
mass_dalton
66148
uniprot
proteins
protein_family
Major facilitator superfamily
uniprot
proteins
protein_family
Sodium/anion cotransporter family
uniprot
proteins
protein_family
VGLUT subfamily
uniprot
proteins
pubmed
12384506
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
18080752
uniprot
proteins
pubmed
18215623
uniprot
proteins
pubmed
18278042
uniprot
proteins
pubmed
18674745
uniprot
proteins
sequence_length
601
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:Q7TSF2}. Cell membrane {ECO:0000250|UniProtKB:Q8NDX2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q8NDX2}. Synapse, synaptosome {ECO:0000250|UniProtKB:Q7TSF2}.
uniprot
proteins
transmembrane_region
TRANSMEM 90..110; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 144..164; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 167..187; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 196..216; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 235..255; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 263..283; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 328..348; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 367..387; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 404..424; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 427..447; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 461..481; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 495..515; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins