Cdc34
entrez
216150
canonical
uniprot
Q8CFI2
canonical
ensembl
ENSMUSG00000020307
resolver
ensp
ENSMUSP00000020550
resolver
ensp
ENSMUSP00000151763
resolver
ensp
ENSMUSP00000152000
resolver
enst
ENSMUST00000020550
resolver
enst
ENSMUST00000219791
resolver
genesymbol
Cdc34
resolver
genesymbol_syn
Ubch3
resolver
genesymbol_syn
Ube2r1
resolver
uniprot
A0A1W2P7U1
resolver
uniprot
Q505K8
resolver
uniprot_entry
A0A1W2P7U1_MOUSE
resolver
uniprot_entry
UB2R1_MOUSE
resolver
uniprot_sec
Q505K8
resolver
activity_regulation
ACTIVITY REGULATION: CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8 to the CUL1 SCF E3 ligase complex subunit. {ECO:0000250|UniProtKB:P49427}.
uniprot
proteins
amino_acid_sequence
MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEADSCFGDEEDDSGTEES
uniprot
proteins
ec_number
2.3.2.23
uniprot
proteins
ec_number
2.3.2.24
uniprot
proteins
function
FUNCTION: E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (By similarity). In vitro catalyzes 'Lys-48'-linked polyubiquitination (By similarity). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:10230406). Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1 (By similarity). Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation (By similarity). Also involved in the degradation of beta-catenin (By similarity). {ECO:0000250|UniProtKB:P49427, ECO:0000269|PubMed:10230406}.
uniprot
proteins
mass_dalton
26622
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination. {ECO:0000250|UniProtKB:P49427}.
uniprot
proteins
post_translational_modification
PTM: Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controls the nuclear localization. {ECO:0000250|UniProtKB:P49427}.
uniprot
proteins
protein_family
Ubiquitin-conjugating enzyme family
uniprot
proteins
pubmed
10230406
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
32640224
uniprot
proteins
sequence_length
235
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P49427}. Nucleus {ECO:0000250|UniProtKB:P49427}. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase. {ECO:0000250|UniProtKB:P49427}.
uniprot
proteins