Epas1
entrez
13819
canonical
uniprot
P97481
canonical
ensembl
ENSMUSG00000024140
resolver
ensp
ENSMUSP00000024954
resolver
enst
ENSMUST00000024954
resolver
genesymbol
Epas1
resolver
genesymbol_syn
Hif2a
resolver
uniprot
O08787
resolver
uniprot
O55046
resolver
uniprot_entry
EPAS1_MOUSE
resolver
uniprot_sec
O08787
resolver
uniprot_sec
O55046
resolver
amino_acid_sequence
MTADKEKKRSSSELRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLTLKNGSGFGKKSKDVSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSSLCGSKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSDDVAVTEKSNYLFTKLKEEPEELAQLAPTPGDAIISLDFGSQNFDEPSAYGKAILPPGQPWVSGLRSHSAQSESGSLPAFTVPQADTPGNTTPSASSSSSCSTPSSPEDYYSSLENPLKIEVIEKLFAMDTEPRDPGSTQTDFSELDLETLAPYIPMDGEDFQLSPICPEEPLMPESPQPTPQHCFSTMTSIFQPLTPGATHGPFFLDKYPQQLESRKTESEHWPMSSIFFDAGSKGSLSPCCGQASTPLSSMGGRSNTQWPPDPPLHFGPTKWPVGDQSAESLGALPVGSSQLEPPSAPPHVSMFKMRSAKDFGARGPYMMSPAMIALSNKLKLKRQLEYEEQAFQDTSGGDPPGTSSSHLMWKRMKSLMGGTCPLMPDKTISANMAPDEFTQKSMRGLGQPLRHLPPPQPPSTRSSGENAKTGFPPQCYASQFQDYGPPGAQKVSGVASRLLGPSFEPYLLPELTRYDCEVNVPVPGSSTLLQGRDLLRALDQAT
uniprot
proteins
function
FUNCTION: Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). {ECO:0000250, ECO:0000269|PubMed:26245371}.
uniprot
proteins
mass_dalton
96712
uniprot
proteins
mutagenesis
MUTAGEN 23; /note="A->D: Decreases HREDNA binding."; /evidence="ECO:0000269|PubMed:26245371"; MUTAGEN 27; /note="R->A: Decreases HREDNA binding."; /evidence="ECO:0000269|PubMed:26245371"; MUTAGEN 169; /note="F->D: Decreases heterodimer formation with ARNT."; /evidence="ECO:0000269|PubMed:26245371"; MUTAGEN 171; /note="R->A: Markedly decreases heterodimer formation with ARNT. Impairs heterodimer formation with ARNT; when associated with D-192."; /evidence="ECO:0000269|PubMed:26245371"; MUTAGEN 184; /note="N->D: Decreases HREDNA binding; when associated with D-186."; /evidence="ECO:0000269|PubMed:26245371"; MUTAGEN 186; /note="K->D: Decreases HREDNA binding; when associated with D-184."; /evidence="ECO:0000269|PubMed:26245371"; MUTAGEN 192; /note="V->D: Markedly decreases heterodimer formation with ARNT. Impairs heterodimer formation with ARNT; when associated with A-171."; /evidence="ECO:0000269|PubMed:26245371"; MUTAGEN 194; /note="H->A: Decreases heterodimer formation with ARNT."; /evidence="ECO:0000269|PubMed:26245371"; MUTAGEN 530; /note="P->A: Confers transcriptional activity at normoxia; when associated with A-851."; /evidence="ECO:0000269|PubMed:12080085"; MUTAGEN 844; /note="T->A: Decreases interaction with CREBBP."; /evidence="ECO:0000269|PubMed:11983697"; MUTAGEN 851; /note="N->A: Confers transcriptional activity at normoxia; when associated with A-530."; /evidence="ECO:0000269|PubMed:12080085
uniprot
proteins
post_translational_modification
PTM: In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). {ECO:0000250}.; PTM: In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. {ECO:0000269|PubMed:11823643}.; PTM: Phosphorylated on multiple sites in the CTAD. {ECO:0000269|PubMed:11983697}.; PTM: The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. {ECO:0000269|PubMed:11823643}.
uniprot
proteins
pubmed
11823643
uniprot
proteins
pubmed
11983697
uniprot
proteins
pubmed
12080085
uniprot
proteins
pubmed
21546903
uniprot
proteins
pubmed
26245371
uniprot
proteins
pubmed
9000051
uniprot
proteins
pubmed
9113979
uniprot
proteins
pubmed
9178256
uniprot
proteins
sequence_length
874
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:21546903}. Nucleus speckle {ECO:0000269|PubMed:21546903}. Note=Colocalizes with HIF3A isoform 2 in the nucleus and speckles. {ECO:0000269|PubMed:21546903}.
uniprot
proteins