Hmces
entrez
500251
canonical
uniprot
Q5XIJ1
canonical
ensembl
ENSRNOG00000079966
resolver
ensembl
ENSRNOG00055012800
resolver
ensembl
ENSRNOG00060024371
resolver
ensembl
ENSRNOG00065015164
resolver
ensp
ENSRNOP00000054105
resolver
ensp
ENSRNOP00055017726
resolver
ensp
ENSRNOP00060035000
resolver
ensp
ENSRNOP00065019656
resolver
enst
ENSRNOT00000057286
resolver
enst
ENSRNOT00055021883
resolver
enst
ENSRNOT00060042206
resolver
enst
ENSRNOT00065025115
resolver
genesymbol
Hmces
resolver
genesymbol_syn
Srapd1
resolver
uniprot_entry
HMCES_RAT
resolver
activity_regulation
ACTIVITY REGULATION: Formation and reversal of DNA-protein cross-link depends on DNA context. Catalyzes formation of the thiazolidine linkage in presence of abasic sites in single-stranded DNA. Mediates the reversal of the thiazolidine cross-link in presence of double stranded DNA. {ECO:0000250|UniProtKB:Q96FZ2}.
uniprot
proteins
amino_acid_sequence
MCGRTSCHLPRDALTRACAYLDRQGRRQLPQWRDPDKYCPSYNKSPQSSSPVLLSRLHFEKDADSSDRIIFPMRWGLVPSWFKESDPSKLQFNTSNCRSDTIMEKQSFKAPLGKGRRCVVLADGFYEWQRCQGTNQRQPYFIYFPQSKTEKSGENSGSDSLNNKEEVWDNWRLLTMAGIFDCWEPPKGERLYSYSIITVDSCRGLSDIHSRMPAILDGEEAVSKWLDFGEVSTQEALKLIHPIDNITFHPVSPVVNNSRNNTPECLAPADLLVKKEPKASGSSQRMMQWLATKSPKKEVPDSPKKDASGLPQWSSQFLQKSPLPTKRGASSSLLDRWLKQEKEDEPVAKRPNS
uniprot
proteins
ec_number
3.4.-.-
uniprot
proteins
ec_number
4.-.-.-
uniprot
proteins
function
FUNCTION: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. Promotes error-free repair by protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks. The HMCES DNA-protein cross-link is then either reversed or degraded. HMCES is able to catalyze the reversal of its thiazolidine cross-link and cycle between a cross-link and a non-cross-linked state depending on DNA context: mediates self-reversal of the thiazolidine cross-link in double stranded DNA, allowing APEX1 to initiate downstream repair of abasic sites. The HMCES DNA-protein cross-link can also be degraded by the SPRTN metalloprotease following unfolding by the BRIP1/FANCJ helicase. Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction. Plays a protective role during somatic hypermutation of immunoglobulin genes in B-cells: acts via its ability to form covalent cross-links with abasic sites, thereby limiting the accumulation of deletions in somatic hypermutation target regions (By similarity). Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA-protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity). {ECO:0000250|UniProtKB:Q8R1M0, ECO:0000250|UniProtKB:Q96FZ2}.
uniprot
proteins
mass_dalton
40222
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated; the covalent HMCES DNA-protein cross-link is ubiquitinated, leading to its degradation by the proteasome. {ECO:0000250|UniProtKB:Q96FZ2}.
uniprot
proteins
protein_family
SOS response-associated peptidase family
uniprot
proteins
pubmed
15489334
uniprot
proteins
sequence_length
353
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Chromosome {ECO:0000250|UniProtKB:Q96FZ2}. Note=Recruited to chromatin following DNA damage. Localizes to replication forks. {ECO:0000250|UniProtKB:Q96FZ2}.
uniprot
proteins