RSAD2
entrez
91543
canonical
uniprot
Q8WXG1
canonical
ensembl
ENSG00000134321
resolver
ensp
ENSP00000371471
resolver
ensp
ENSP00000406427
resolver
ensp
ENSP00000506390
resolver
enst
ENST00000382040
resolver
enst
ENST00000442639
resolver
enst
ENST00000680607
resolver
genesymbol
RSAD2
resolver
genesymbol_syn
CIG5
resolver
hgnc
HGNC:30908
resolver
uniprot
C9J674
resolver
uniprot
Q8WVI4
resolver
uniprot_entry
C9J674_HUMAN
resolver
uniprot_entry
RSAD2_HUMAN
resolver
uniprot_sec
Q8WVI4
resolver
activity_regulation
ACTIVITY REGULATION: IRAK1 and TRAF6 synergistically activate RSAD2 increasing its activity with CTP as substrate about 10-fold. {ECO:0000269|PubMed:30872404}.
uniprot
proteins
amino_acid_sequence
MWVLTPAAFAGKLLSVFRQPLSSLWRSLVPLFCWLRATFWLLATKRRKQQLVLRGPDETKEEEEDPPLPTTPTSVNYHFTRQCNYKCGFCFHTAKTSFVLPLEEAKRGLLLLKEAGMEKINFSGGEPFLQDRGEYLGKLVRFCKVELRLPSVSIVSNGSLIRERWFQNYGEYLDILAISCDSFDEEVNVLIGRGQGKKNHVENLQKLRRWCRDYRVAFKINSVINRFNVEEDMTEQIKALNPVRWKVFQCLLIEGENCGEDALREAERFVIGDEEFERFLERHKEVSCLVPESNQKMKDSYLILDEYMRFLNCRKGRKDPSKSILDVGVEEAIKFSGFDEKMFLKRGGKYIWSKADLKLDW
uniprot
proteins
ec_number
4.2.-.-
uniprot
proteins
function
FUNCTION: Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon (PubMed:31812350). Catalyzes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP) via a SAM-dependent radical mechanism (PubMed:29925952, PubMed:30872404). In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication (PubMed:29925952). Therefore, inhibits a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), zika virus, and human immunodeficiency virus (HIV-1) (PubMed:29925952, PubMed:30587778, PubMed:30934824, PubMed:31921110). Also promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6 (PubMed:30872404). Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. {ECO:0000269|PubMed:11752458, ECO:0000269|PubMed:16108059, ECO:0000269|PubMed:16982913, ECO:0000269|PubMed:17686841, ECO:0000269|PubMed:18005719, ECO:0000269|PubMed:19074433, ECO:0000269|PubMed:29925952, ECO:0000269|PubMed:30587778, ECO:0000269|PubMed:30872404, ECO:0000269|PubMed:30934824, ECO:0000269|PubMed:31812350, ECO:0000269|PubMed:31921110}.
uniprot
proteins
mass_dalton
42170
uniprot
proteins
mutagenesis
MUTAGEN 83; /note="C->A: Loss of ability to assemble an Fe-S cluster and significant decrease in protein stability."; /evidence="ECO:0000269|PubMed:22363738"; MUTAGEN 87; /note="C->A: Loss of ability to assemble an Fe-S cluster and significant decrease in protein stability."; /evidence="ECO:0000269|PubMed:22363738"; MUTAGEN 90; /note="C->A: Loss of ability to assemble an Fe-S cluster and significant decrease in protein stability."; /evidence="ECO:0000269|PubMed:22363738"; MUTAGEN 197; /note="K->R: Loss of acetylation."; /evidence="ECO:0000269|PubMed:31812350"; MUTAGEN 206; /note="K->R: Loss of Lys-6-linked ubiquitination."; /evidence="ECO:0000269|PubMed:31812350"; MUTAGEN 358; /note="K->A: Complete loss of antiviral activity against Zika virus."; /evidence="ECO:0000269|PubMed:30934824
uniprot
proteins
post_translational_modification
PTM: Acetylated by HAT1. HAT1-mediated acetylation of Lys-197 in turn recruits UBE4A that stimulates RSAD2 polyubiquitination leading to proteasomal degradation. {ECO:0000269|PubMed:31812350}.; PTM: 'Lys-6'-linked polyubiquitination at Lys-206 leads to RSAD2 protein degradation. {ECO:0000269|PubMed:31812350}.
uniprot
proteins
protein_family
RSAD2 family
uniprot
proteins
protein_family
Radical SAM superfamily
uniprot
proteins
pubmed
11752458
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15815621
uniprot
proteins
pubmed
15890971
uniprot
proteins
pubmed
16108059
uniprot
proteins
pubmed
16150475
uniprot
proteins
pubmed
16849320
uniprot
proteins
pubmed
16982913
uniprot
proteins
pubmed
17686841
uniprot
proteins
pubmed
18005719
uniprot
proteins
pubmed
18005724
uniprot
proteins
pubmed
19074433
uniprot
proteins
pubmed
20026307
uniprot
proteins
pubmed
20176015
uniprot
proteins
pubmed
21142818
uniprot
proteins
pubmed
21527675
uniprot
proteins
pubmed
21957124
uniprot
proteins
pubmed
22177558
uniprot
proteins
pubmed
22182524
uniprot
proteins
pubmed
22363738
uniprot
proteins
pubmed
29925952
uniprot
proteins
pubmed
30587778
uniprot
proteins
pubmed
30872404
uniprot
proteins
pubmed
30934824
uniprot
proteins
pubmed
31812350
uniprot
proteins
pubmed
31921110
uniprot
proteins
pubmed
36387278
uniprot
proteins
pubmed
9391139
uniprot
proteins
sequence_length
361
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus {ECO:0000269|PubMed:11752458, ECO:0000269|PubMed:16982913, ECO:0000269|PubMed:31921110}. Endoplasmic reticulum {ECO:0000269|PubMed:11752458, ECO:0000269|PubMed:16982913, ECO:0000269|PubMed:30587778}. Lipid droplet {ECO:0000269|PubMed:31921110}. Mitochondrion. Mitochondrion inner membrane. Mitochondrion outer membrane. Note=Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28. Interaction with human cytomegalovirus/HHV-5 protein vMIA/UL37 results in its relocalization from the endoplasmic reticulum to the mitochondria.
uniprot
proteins