ANKLE1
entrez
126549
canonical
uniprot
Q8NAG6
canonical
ensembl
ENSG00000160117
resolver
ensp
ENSP00000377971
resolver
ensp
ENSP00000384008
resolver
ensp
ENSP00000470895
resolver
ensp
ENSP00000498416
resolver
enst
ENST00000394458
resolver
enst
ENST00000652132
resolver
genesymbol
ANKLE1
resolver
genesymbol_syn
ANKRD41
resolver
genesymbol_syn
LEM3
resolver
hgnc
HGNC:26812
resolver
uniprot
A0A494C092
resolver
uniprot
A0A499FJM0
resolver
uniprot
A8VU82
resolver
uniprot
B4DSY8
resolver
uniprot
B4E124
resolver
uniprot
Q8N8J8
resolver
uniprot_entry
A0A494C092_HUMAN
resolver
uniprot_entry
A0A499FJM0_HUMAN
resolver
uniprot_entry
ANKL1_HUMAN
resolver
uniprot_entry
B4DSY8_HUMAN
resolver
uniprot_entry
B4E124_HUMAN
resolver
uniprot_sec
A8VU82
resolver
uniprot_sec
Q8N8J8
resolver
amino_acid_sequence
MCSEARLARRLRDALREEEPWAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGENPHPITPFTRQLYHQQLEEAQIAPGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG
uniprot
proteins
ec_number
3.1.-.-
uniprot
proteins
function
FUNCTION: Structure-selective DNA endonuclease that specifically cleaves branched DNA (PubMed:22399800, PubMed:27245214, PubMed:32866453, PubMed:36825683, PubMed:37216589). Processes chromatin bridges during cytokinesis by cleaving DNA, both priming TREX1-mediated resolution and directly resolving bridges (PubMed:36825683). Proper processing of chromatin bridges prevents their breakage during cytokinesis, thereby reducing DNA damage, micronuclei formation, and the activation of immune responses such as the cGAS-STING pathway (PubMed:36825683). Acts as a DNA tension sensor that resolves stretched chromatin bridges during cell division by cleaving mechanically stressed and supercoiled DNA (PubMed:41361016). Its activity increases in response to mechanical stress and negative supercoiling, specifically cleaving both strands of negatively supercoiled DNA at higher stretching forces (PubMed:41361016). {ECO:0000269|PubMed:22399800, ECO:0000269|PubMed:27245214, ECO:0000269|PubMed:32866453, ECO:0000269|PubMed:36825683, ECO:0000269|PubMed:37216589}.
uniprot
proteins
mass_dalton
66890
uniprot
proteins
mutagenesis
MUTAGEN 110..117; /note="LRPLDLAL->ARPADAAA: Slight increase in nuclear localization."; /evidence="ECO:0000269|PubMed:27245214"; MUTAGEN 271..280; /note="LNARLQALTL->ANARAQAATA: Increased nuclear localization."; /evidence="ECO:0000269|PubMed:27245214"; MUTAGEN 453; /note="Y->A: Loss of endonucleolytic activity."; /evidence="ECO:0000269|PubMed:22399800"; MUTAGEN 453; /note="Y->F: Loss of endonucleolytic activity."; /evidence="ECO:0000269|PubMed:32866453, ECO:0000269|PubMed:37216589"; MUTAGEN 486..488; /note="YVG->AAA: Probable loss of endonucleolytic activity. Fails to induce DNA damage response upon leptomycin-mediated nuclear localization. No nuclear translocation upon treatment with DNA damaging agents. Steady state cytoplasmic localization is not affected."; /evidence="ECO:0000269|PubMed:27245214"; MUTAGEN 486; /note="Y->F: Loss of endonucleolytic activity."; /evidence="ECO:0000269|PubMed:32866453, ECO:0000269|PubMed:37216589"; MUTAGEN 488; /note="G->A: Loss of endonucleolytic activity."; /evidence="ECO:0000269|PubMed:22399800"; MUTAGEN 498; /note="H->A: Reduced endonucleolytic activity."; /evidence="ECO:0000269|PubMed:37216589"; MUTAGEN 519; /note="K->A: Reduced endonucleolytic activity."; /evidence="ECO:0000269|PubMed:37216589"; MUTAGEN 551; /note="E->A: Loss of endonucleolytic activity."; /evidence="ECO:0000269|PubMed:22399800, ECO:0000269|PubMed:37216589"; MUTAGEN 565; /note="N->D: Loss of endonucleolytic activity."; /evidence="ECO:0000269|PubMed:37216589
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15057824
uniprot
proteins
pubmed
22399800
uniprot
proteins
pubmed
27245214
uniprot
proteins
pubmed
32866453
uniprot
proteins
pubmed
36825683
uniprot
proteins
pubmed
37216589
uniprot
proteins
pubmed
41361016
uniprot
proteins
sequence_length
615
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22399800, ECO:0000269|PubMed:27245214}. Nucleus {ECO:0000269|PubMed:22399800, ECO:0000269|PubMed:27245214}. Midbody {ECO:0000269|PubMed:36825683, ECO:0000269|PubMed:41361016}. Note=At the steady state, localizes predominantly in the cytoplasm (PubMed:22399800, PubMed:27245214). Localizes to the midbody in a KIF23 and PRC1-dependent manner (PubMed:36825683). {ECO:0000269|PubMed:22399800, ECO:0000269|PubMed:27245214, ECO:0000269|PubMed:36825683}.
uniprot
proteins