Izumo1
entrez
73456
canonical
uniprot
Q9D9J7
canonical
ensembl
ENSMUSG00000064158
resolver
ensp
ENSMUSP00000033100
resolver
enst
ENSMUST00000033100
resolver
genesymbol
Izumo1
resolver
uniprot
A0A455R725
resolver
uniprot_entry
A0A455R725_MOUSE
resolver
uniprot_entry
IZUM1_MOUSE
resolver
amino_acid_sequence
MGPHFTLLLAALANCLCPGRPCIKCDQFVTDALKTFENTYLNDHLPHDIHKNVMRMVNHEVSSFGVVTSAEDSYLGAVDENTLEQATWSFLKDLKRITDSDLKGELFIKELLWMLRHQKDIFNNLARQFQKEVLCPNKCGVMSQTLIWCLKCEKQLHICRKSLDCGERHIEVHRSEDLVLDCLLSWHRASKGLTDYSFYRVWENSSETLIAKGKEPYLTKSMVGPEDAGNYRCVLDTINQGHATVIRYDVTVLPPKHSEENQPPNIITQEEHETPVHVTPQTPPGQEPESELYPELHPELYPELIPTVAQNPEKKMKTRLLILLTLGFVVLVASIIISVLHFRKVSAKLKNASDEVKPTASGSKSDQSLSQQMGLKKASQADFNSDYSGDKSEATEN
uniprot
proteins
function
FUNCTION: Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg (PubMed:15759005, PubMed:24739963, PubMed:27309808). The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion (PubMed:15759005, PubMed:24739963, PubMed:27309808). The ligand-receptor interaction probably does not act as a membrane 'fusogen' (PubMed:15759005, PubMed:24739963, PubMed:27309808). Plays a critical role in sperm-oolemma binding prior to plasma membrane fusion (PubMed:35096839). Can mediate cell-cell fusion in cultured mammalian cells independently of its binding to IZUMO1R/JUNO (PubMed:36394541). {ECO:0000269|PubMed:15759005, ECO:0000269|PubMed:24739963, ECO:0000269|PubMed:27309808, ECO:0000269|PubMed:35096839, ECO:0000269|PubMed:36394541}.
uniprot
proteins
mass_dalton
44885
uniprot
proteins
mutagenesis
MUTAGEN 28; /note="F->A: No effect on localization to cell membrane or interaction with IZUMO1R but loss of its ability to mediate cell-cell fusion; when associated with A-88 and A-113."; /evidence="ECO:0000269|PubMed:36394541"; MUTAGEN 88; /note="W->A: No effect on localization to cell membrane or interaction with IZUMO1R but loss of its ability to mediate cell-cell fusion; when associated with A-28 and A-113."; /evidence="ECO:0000269|PubMed:36394541"; MUTAGEN 113; /note="W->A: No effect on localization to cell membrane or interaction with IZUMO1R but loss of its ability to mediate cell-cell fusion; when associated with A-28 and A-88."; /evidence="ECO:0000269|PubMed:36394541"; MUTAGEN 148; /note="W->A: Abolishes adhesion to oocytes. Loss of interaction with IZUMO1R but no effect on localization to cell membrane or its ability to mediate cell-cell fusion."; /evidence="ECO:0000269|PubMed:27309808, ECO:0000269|PubMed:36394541"; MUTAGEN 154; /note="K->A: Decreases adhesion to oocytes."; /evidence="ECO:0000269|PubMed:27309808"; MUTAGEN 157; /note="H->A: Abolishes adhesion to oocytes."; /evidence="ECO:0000269|PubMed:27309808"; MUTAGEN 158; /note="I->R: Strongly decreases adhesion to oocytes."; /evidence="ECO:0000269|PubMed:27309808"; MUTAGEN 160; /note="R->A: Abolishes adhesion to oocytes."; /evidence="ECO:0000269|PubMed:27309808"; MUTAGEN 163; /note="L->A: Decreases adhesion to oocytes."; /evidence="ECO:0000269|PubMed:27309808"; MUTAGEN 204; /note="N->Q: Almost no change in fusion-facilitating activity."; /evidence="ECO:0000269|PubMed:18952059
uniprot
proteins
post_translational_modification
PTM: N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm. {ECO:0000269|PubMed:18952059}.; PTM: Phosphorylated (PubMed:19658160, PubMed:27624483). The cytoplasmic C-terminus is phosphorylated and undergoes phosphorylation changes during epididymal transit (PubMed:27624483). {ECO:0000269|PubMed:19658160, ECO:0000269|PubMed:27624483}.
uniprot
proteins
protein_family
Izumo family
uniprot
proteins
pubmed
15759005
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
18952059
uniprot
proteins
pubmed
19658160
uniprot
proteins
pubmed
20421979
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
24739963
uniprot
proteins
pubmed
25209248
uniprot
proteins
pubmed
26568141
uniprot
proteins
pubmed
27309808
uniprot
proteins
pubmed
27374339
uniprot
proteins
pubmed
27624483
uniprot
proteins
pubmed
29954238
uniprot
proteins
pubmed
30517645
uniprot
proteins
pubmed
31672133
uniprot
proteins
pubmed
32393636
uniprot
proteins
pubmed
32484434
uniprot
proteins
pubmed
35096839
uniprot
proteins
pubmed
35618043
uniprot
proteins
pubmed
35960805
uniprot
proteins
pubmed
36394541
uniprot
proteins
sequence_length
397
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:25209248, ECO:0000269|PubMed:27309808, ECO:0000269|PubMed:36394541, ECO:0000305|PubMed:19658160}; Single-pass type I membrane protein {ECO:0000255}. Cytoplasmic vesicle, secretory vesicle, acrosome membrane {ECO:0000269|PubMed:20421979, ECO:0000269|PubMed:27309808, ECO:0000269|PubMed:31672133, ECO:0000269|PubMed:32393636, ECO:0000269|PubMed:32484434}; Single-pass type I membrane protein {ECO:0000255}. Note=Localizes initially to the acrosome membrane of the sperm head (both outer and inner acrosomal membranes). During the acrosome reaction, translocates to the plasma membrane. {ECO:0000269|PubMed:27624483, ECO:0000269|PubMed:31672133}.
uniprot
proteins
transmembrane_region
TRANSMEM 320..340; /note="Helical"; /evidence="ECO:0000255
uniprot
proteins