QTRT1
entrez
81890
canonical
uniprot
Q9BXR0
canonical
ensembl
ENSG00000213339
resolver
ensp
ENSP00000250237
resolver
genesymbol
QTRT1
resolver
genesymbol_syn
TGT
resolver
genesymbol_syn
TGUT
resolver
hgnc
HGNC:23797
resolver
uniprot
B4DFM7
resolver
uniprot
Q71RF8
resolver
uniprot
Q96BQ4
resolver
uniprot
Q9BXQ9
resolver
uniprot_entry
Q71RF8_HUMAN
resolver
uniprot_entry
TGT_HUMAN
resolver
uniprot_sec
B4DFM7
resolver
uniprot_sec
Q96BQ4
resolver
uniprot_sec
Q9BXQ9
resolver
amino_acid_sequence
MAGAATQASLESAPRIMRLVAECSRSRARAGELWLPHGTVATPVFMPVGTQATMKGITTEQLDALGCRICLGNTYHLGLRPGPELIQKANGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGVRFRSPYDGNETLLSPEKSVQIQNALGSDIIMQLDDVVSSTVTGPRVEEAMYRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLRATCLEEMTKRDVPGFAIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKHSRAFLHALLHSDNTAALHHLTVHNIAYQLQLMSAVRTSIVEKRFPDFVRDFMGAMYGDPTLCPTWATDALASVGITLG
uniprot
proteins
ec_number
2.4.2.64
uniprot
proteins
function
FUNCTION: Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154, PubMed:34009357, PubMed:34241577). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity). Modification of cytoplasmic tRNAs with queuosine controls the elongation speed of cognate codons, thereby ensuring the correct folding of nascent proteins to maintain proteome integrity (PubMed:30093495). {ECO:0000255|HAMAP-Rule:MF_03218, ECO:0000269|PubMed:11255023, ECO:0000269|PubMed:20354154, ECO:0000269|PubMed:30093495, ECO:0000269|PubMed:34009357, ECO:0000269|PubMed:34241577}.
uniprot
proteins
mass_dalton
44048
uniprot
proteins
mutagenesis
MUTAGEN 279; /note="D->N: Loss of transglycosylase activity."; /evidence="ECO:0000269|PubMed:20354154
uniprot
proteins
protein_family
Queuine tRNA-ribosyltransferase family
uniprot
proteins
pubmed
11255023
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15057824
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
20354154
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22814378
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
30093495
uniprot
proteins
pubmed
30149595
uniprot
proteins
pubmed
34009357
uniprot
proteins
pubmed
34241577
uniprot
proteins
sequence_length
403
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03218}. Mitochondrion outer membrane {ECO:0000255|HAMAP-Rule:MF_03218}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03218}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03218}. Note=Weakly associates with mitochondria, possibly via QTRT2. {ECO:0000255|HAMAP-Rule:MF_03218}.
uniprot
proteins