QK3
entrez
9444
canonical
uniprot
Q96PU8
canonical
ensembl
ENSG00000112531
resolver
ensp
ENSP00000275262
resolver
ensp
ENSP00000354867
resolver
ensp
ENSP00000355094
resolver
ensp
ENSP00000375973
resolver
ensp
ENSP00000408775
resolver
ensp
ENSP00000543439
resolver
ensp
ENSP00000664332
resolver
ensp
ENSP00000744833
resolver
ensp
ENSP00000744835
resolver
genesymbol
QK3
resolver
genesymbol
QKI
resolver
genesymbol_syn
HKQ
resolver
hgnc
HGNC:21100
resolver
uniprot
Q2I375
resolver
uniprot
Q5MJQ1
resolver
uniprot
Q8WY44
resolver
uniprot
Q969L9
resolver
uniprot
Q96EJ3
resolver
uniprot
Q96KA3
resolver
uniprot
Q96PU6
resolver
uniprot
Q96PU7
resolver
uniprot
Q9P0X6
resolver
uniprot
Q9P0X7
resolver
uniprot
Q9P0X8
resolver
uniprot
Q9P0X9
resolver
uniprot
Q9P0Y0
resolver
uniprot
Q9P0Y1
resolver
uniprot_entry
Q8WY44_HUMAN
resolver
uniprot_entry
QKI_HUMAN
resolver
uniprot_sec
Q2I375
resolver
uniprot_sec
Q5MJQ1
resolver
uniprot_sec
Q969L9
resolver
uniprot_sec
Q96EJ3
resolver
uniprot_sec
Q96KA3
resolver
uniprot_sec
Q96PU6
resolver
uniprot_sec
Q96PU7
resolver
uniprot_sec
Q9P0X6
resolver
uniprot_sec
Q9P0X7
resolver
uniprot_sec
Q9P0X8
resolver
uniprot_sec
Q9P0X9
resolver
uniprot_sec
Q9P0Y0
resolver
uniprot_sec
Q9P0Y1
resolver
amino_acid_sequence
MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN
uniprot
proteins
function
FUNCTION: RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as pre-mRNA splicing, circular RNA (circRNA) formation, mRNA export, mRNA stability and/or translation (PubMed:22398723, PubMed:23630077, PubMed:25768908, PubMed:27029405, PubMed:31331967, PubMed:37379838). Involved in various cellular processes, such as mRNA storage into stress granules, apoptosis, lipid deposition, interferon response, glial cell fate and development (PubMed:25768908, PubMed:31829086, PubMed:34428287, PubMed:37379838). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence (PubMed:23630077). Acts as a mRNA modification reader that specifically recognizes and binds mRNA transcripts modified by internal N(7)-methylguanine (m7G) (PubMed:37379838). Promotes the formation of circular RNAs (circRNAs) during the epithelial to mesenchymal transition and in cardiomyocytes: acts by binding to sites flanking circRNA-forming exons (PubMed:25768908). CircRNAs are produced by back-splicing circularization of pre-mRNAs (PubMed:25768908). Plays a central role in myelinization via 3 distinct mechanisms (PubMed:16641098). First, acts by protecting and promoting stability of target mRNAs such as MBP, SIRT2 and CDKN1B, which promotes oligodendrocyte differentiation (By similarity). Second, participates in mRNA transport by regulating the nuclear export of MBP mRNA (By similarity). Finally, indirectly regulates mRNA splicing of MAG pre-mRNA during oligodendrocyte differentiation by acting as a negative regulator of MAG exon 12 alternative splicing: acts by binding to HNRNPA1 mRNA splicing factor, preventing its translation (By similarity). Involved in microglia differentiation and remyelination by regulating microexon alternative splicing of the Rho GTPase pathway (By similarity). Involved in macrophage differentiation: promotes monocyte differentiation by regulating pre-mRNA splicing in naive peripheral blood monocytes (PubMed:27029405). Acts as an important regulator of muscle development: required for the contractile function of cardiomyocytes by regulating alternative splicing of cardiomyocyte transcripts (By similarity). Acts as a negative regulator of thermogenesis by decreasing stability, nuclear export and translation of mRNAs encoding PPARGC1A and UCP1 (By similarity). Also required for visceral endoderm function and blood vessel development (By similarity). May also play a role in smooth muscle development (PubMed:31331967). In addition to its RNA-binding activity, also acts as a nuclear transcription coactivator for SREBF2/SREBP2 (By similarity). {ECO:0000250|UniProtKB:Q9QYS9, ECO:0000269|PubMed:16641098, ECO:0000269|PubMed:22398723, ECO:0000269|PubMed:23630077, ECO:0000269|PubMed:25768908, ECO:0000269|PubMed:27029405, ECO:0000269|PubMed:31331967, ECO:0000269|PubMed:31829086, ECO:0000269|PubMed:34428287, ECO:0000269|PubMed:37379838}.; FUNCTION: [Isoform QKI5]: Nuclear isoform that acts as an indirect regulator of mRNA splicing (By similarity). Regulates mRNA splicing of MAG pre-mRNA by inhibiting translation of HNRNPA1 mRNA, thereby preventing MAG exon 12 alternative splicing (By similarity). Involved in oligodendrocyte differentiation by promoting stabilization of SIRT2 mRNA (By similarity). Acts as a negative regulator of the interferon response by binding to MAVS mRNA, down-regulating its expression (PubMed:31829086). Also inhibits the interferon response by binding to fibrinectin FN1 pre-mRNA, repressing EDA exon inclusion in FN1 (PubMed:34428287). Delays macrophage differentiation by binding to CSF1R mRNA, promoting its degradation (PubMed:22398723). In addition to its RNA-binding activity, also acts as a nuclear transcription coactivator for SREBF2/SREBP2, promoting SREBF2/SREBP2-dependent cholesterol biosynthesis (By similarity). SREBF2/SREBP2-dependent cholesterol biosynthesis participates to myelinization and is required for eye lens transparency (By similarity). {ECO:0000250|UniProtKB:Q9QYS9, ECO:0000269|PubMed:22398723, ECO:0000269|PubMed:31829086, ECO:0000269|PubMed:34428287}.; FUNCTION: [Isoform QKI6]: Cytosolic isoform that specifically recognizes and binds mRNA transcripts modified by internal N(7)-methylguanine (m7G) (PubMed:37379838). Interaction with G3BP1 promotes localization of m7G-containing mRNAs into stress granules in response to stress, thereby suppressing their translation (PubMed:37379838). Acts as a translational repressor for HNRNPA1 and GLI1 (By similarity). Translation inhibition of HNRNPA1 during oligodendrocyte differentiation prevents inclusion of exon 12 in MAG pre-mRNA splicing (By similarity). Involved in astrocyte differentiation by regulating translation of target mRNAs (By similarity). {ECO:0000250|UniProtKB:Q9QYS9, ECO:0000269|PubMed:37379838}.; FUNCTION: [Isoform QKI7]: Cytosolic isoform that specifically recognizes and binds mRNA transcripts modified by internal N(7)-methylguanine (m7G) (PubMed:37379838). Interaction with G3BP1 promotes localization of m7G-containing mRNAs into stress granules in response to stress, thereby suppressing their translation (PubMed:37379838). Acts as a negative regulator of angiogenesis by binding to mRNAs encoding CDH5, NLGN1 and TNFAIP6, promoting their degradation (PubMed:32732889). Can also induce apoptosis in the cytoplasm (By similarity). Heterodimerization with other isoforms results in nuclear translocation of isoform QKI7 and suppression of apoptosis (By similarity). Also binds some microRNAs: promotes stabilitation of miR-122 by mediating recruitment of poly(A) RNA polymerase TENT2, leading to 3' adenylation and stabilization of miR-122 (PubMed:31792053). {ECO:0000250|UniProtKB:Q9QYS9, ECO:0000269|PubMed:31792053, ECO:0000269|PubMed:32732889, ECO:0000269|PubMed:37379838}.
uniprot
proteins
mass_dalton
37671
uniprot
proteins
mutagenesis
MUTAGEN 97; /note="N->A: Decrease in target mRNA abundance and 10-fold decrease in RNA binding affinity; when associated with A-130."; /evidence="ECO:0000269|PubMed:23630077"; MUTAGEN 120; /note="K->A: Decrease in target mRNA abundance and 20-fold decrease in RNA binding affinity; when associated with A-124."; /evidence="ECO:0000269|PubMed:23630077"; MUTAGEN 124; /note="R->A: Decrease in target mRNA abundance and 20-fold decrease in RNA binding affinity; when associated with A-120."; /evidence="ECO:0000269|PubMed:23630077"; MUTAGEN 130; /note="R->A: Decrease in target mRNA abundance and 10-fold decrease in RNA binding affinity; when associated with A-97."; /evidence="ECO:0000269|PubMed:23630077"; MUTAGEN 190; /note="K->A: Decrease in target mRNA abundance and 124-fold decrease in RNA binding affinity; when associated with A-193."; /evidence="ECO:0000269|PubMed:23630077"; MUTAGEN 193; /note="Q->A: Decrease in target mRNA abundance and 124-fold decrease in RNA binding affinity; when associated with A-190."; /evidence="ECO:0000269|PubMed:23630077
uniprot
proteins
post_translational_modification
PTM: Methylated by PRMT1. {ECO:0000250|UniProtKB:Q9QYS9}.; PTM: Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA (By similarity). {ECO:0000250|UniProtKB:Q9QYS9}.; PTM: Ubiquitinated by RNF6 in macrophages, leading to its degradation. {ECO:0000250|UniProtKB:Q9QYS9}.
uniprot
proteins
protein_family
Quaking family
uniprot
proteins
pubmed
11856480
uniprot
proteins
pubmed
12529443
uniprot
proteins
pubmed
14574404
uniprot
proteins
pubmed
14702039
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16342280
uniprot
proteins
pubmed
16641098
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
22398723
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
23455924
uniprot
proteins
pubmed
23630077
uniprot
proteins
pubmed
24129315
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
25768908
uniprot
proteins
pubmed
27029405
uniprot
proteins
pubmed
31331967
uniprot
proteins
pubmed
31792053
uniprot
proteins
pubmed
31829086
uniprot
proteins
pubmed
32732889
uniprot
proteins
pubmed
34428287
uniprot
proteins
pubmed
37379838
uniprot
proteins
sequence_length
341
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:37379838}. Cytoplasm {ECO:0000269|PubMed:37379838}.; SUBCELLULAR LOCATION: [Isoform QKI5]: Nucleus {ECO:0000269|PubMed:37379838}. Cytoplasm {ECO:0000269|PubMed:37379838}. Note=Localizes predominantly in the nucleus and at lower levels in cytoplasm (PubMed:37379838). It shuttles between the cytoplasm and the nucleus (By similarity). {ECO:0000250|UniProtKB:Q9QYS9, ECO:0000269|PubMed:37379838}.; SUBCELLULAR LOCATION: [Isoform QKI6]: Cytoplasm, cytosol {ECO:0000269|PubMed:37379838}. Nucleus {ECO:0000269|PubMed:37379838}. Note=Localizes predominantly in the cytoplasm and at lower levels in nucleus. {ECO:0000269|PubMed:37379838}.; SUBCELLULAR LOCATION: [Isoform QKI7]: Cytoplasm, cytosol {ECO:0000269|PubMed:37379838}. Cytoplasm, Stress granule {ECO:0000269|PubMed:37379838}. Nucleus {ECO:0000269|PubMed:37379838}. Note=Localizes predominantly in the cytoplasm and at much lower levels in nucleus (PubMed:37379838). Shuttles between the cytosol and stress granules in response to stress (PubMed:37379838). {ECO:0000269|PubMed:37379838}.
uniprot
proteins