Nhej1
entrez
75570
canonical
uniprot
Q3KNJ2
canonical
ensembl
ENSMUSG00000026162
resolver
ensp
ENSMUSP00000116797
resolver
genesymbol
Nhej1
resolver
genesymbol_syn
Xlf
resolver
uniprot
A2RT39
resolver
uniprot
Q99JK0
resolver
uniprot
Q9D9U0
resolver
uniprot_entry
NHEJ1_MOUSE
resolver
uniprot_sec
A2RT39
resolver
uniprot_sec
Q99JK0
resolver
uniprot_sec
Q9D9U0
resolver
amino_acid_sequence
MEELEQDLLLQPWAWLQLAENSLLAKVSITKHGYALLISDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAALLCHLDEALRPLFKDSAHPSKATFSCDRGEEGLILRVQSELSGLPFSWHFHCIPASSSLVSQHLIHPLMGVSLALQSHVRELAALLRMKDLEIQAYQESGAVLSRSRLKTEPFEENSFLEQFMAEKLPEACAVGDGKPFAMSLQSLYVAVTKQQIQARQAHKDSGETQASSSTSPRGTDNQPEEPVSLSSTLSEPEYEPVAASGPMHRARLVKSKRKKPRGLFS
uniprot
proteins
function
FUNCTION: DNA repair protein involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination (PubMed:17360556, PubMed:27601299, PubMed:27601633, PubMed:27798842, PubMed:27830975, PubMed:28051062, PubMed:29077092, PubMed:30017584). It is also involved in telomere maintenance (By similarity). Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends (PubMed:17360556). Interacts with POLL (DNA polymerase lambda); promoting POLL recruitment to double-strand breaks (DSBs) and stimulation of the end-filling activity of POLL (By similarity). May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary (PubMed:17360556). In some studies, has been shown to associate with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired (By similarity). Alternatively, it has also been shown that rather than forming filaments, a single NHEJ1 dimer interacts through both head domains with XRCC4 to promote the close alignment of DNA ends (By similarity). The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other (By similarity). The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors (By similarity). Binds DNA in a length-dependent manner (By similarity). {ECO:0000250|UniProtKB:A0A1L8ENT6, ECO:0000250|UniProtKB:Q9H9Q4, ECO:0000269|PubMed:17360556, ECO:0000269|PubMed:27601299, ECO:0000269|PubMed:27601633, ECO:0000269|PubMed:27798842, ECO:0000269|PubMed:27830975, ECO:0000269|PubMed:28051062, ECO:0000269|PubMed:29077092, ECO:0000269|PubMed:30017584}.
uniprot
proteins
mass_dalton
32739
uniprot
proteins
post_translational_modification
PTM: Phosphorylated by PRKDC at the C-terminus in response to DNA damage. Phosphorylation by PRKDC at the C-terminus of XRCC4 and NHEJ1/XLF are highly redundant and regulate ability of the XRCC4-NHEJ1/XLF subcomplex to bridge DNA. Phosphorylation does not prevent interaction with XRCC4 but disrupts ability to bridge DNA and promotes detachment from DNA. {ECO:0000250|UniProtKB:Q9H9Q4}.
uniprot
proteins
protein_family
XLF subfamily
uniprot
proteins
protein_family
XRCC4-XLF family
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
17360556
uniprot
proteins
pubmed
18775323
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
27601299
uniprot
proteins
pubmed
27601633
uniprot
proteins
pubmed
27798842
uniprot
proteins
pubmed
27830975
uniprot
proteins
pubmed
28051062
uniprot
proteins
pubmed
29077092
uniprot
proteins
pubmed
30017584
uniprot
proteins
sequence_length
295
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H9Q4}. Chromosome {ECO:0000250|UniProtKB:Q9H9Q4}. Note=Localizes to site of double-strand breaks; recruitment is dependent on XRCC5-XRCC6 (Ku) heterodimer. {ECO:0000250|UniProtKB:Q9H9Q4}.
uniprot
proteins