OXSR1
entrez
9943
canonical
uniprot
O95747
canonical
ensembl
ENSG00000172939
resolver
ensp
ENSP00000311713
resolver
ensp
ENSP00000525402
resolver
enst
ENST00000311806
resolver
genesymbol
OXSR1
resolver
genesymbol_syn
KIAA1101
resolver
genesymbol_syn
OSR1
resolver
hgnc
HGNC:8508
resolver
uniprot
Q3LR53
resolver
uniprot
Q7Z501
resolver
uniprot
Q9UPQ1
resolver
uniprot_entry
OXSR1_HUMAN
resolver
uniprot_sec
Q3LR53
resolver
uniprot_sec
Q7Z501
resolver
uniprot_sec
Q9UPQ1
resolver
activity_regulation
ACTIVITY REGULATION: Activated following phosphorylation by WNK kinases (WNK1, WNK2, WNK3 or WNK4). {ECO:0000269|PubMed:16669787, ECO:0000269|PubMed:16832045, ECO:0000269|PubMed:17190791, ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:22989884, ECO:0000269|PubMed:23386621, ECO:0000269|PubMed:29581290, ECO:0000269|PubMed:34289367}.
uniprot
proteins
amino_acid_sequence
MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS
uniprot
proteins
ec_number
2.7.11.1
uniprot
proteins
function
FUNCTION: Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed:16669787, PubMed:18270262, PubMed:21321328, PubMed:34289367). Specifically recognizes and binds proteins with a RFXV motif (PubMed:16669787, PubMed:17721439, PubMed:21321328). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed:17721439). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (PubMed:16669787, PubMed:21321328). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (PubMed:16669787, PubMed:19665974, PubMed:21321328). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed:18270262). Also acts as a regulator of angiogenesis in endothelial cells downstream of WNK1 (PubMed:23386621, PubMed:25362046). Acts as an activator of inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1: recognizes and binds the RXFXV/I variant motif on KCNJ2/Kir2.1 and KCNJ4/Kir2.3 and regulates their localization to the cell membrane without mediating their phosphorylation (PubMed:29581290). Phosphorylates RELL1, RELL2 and RELT (PubMed:16389068, PubMed:28688764). Phosphorylates PAK1 (PubMed:14707132). Phosphorylates PLSCR1 in the presence of RELT (PubMed:22052202). {ECO:0000269|PubMed:14707132, ECO:0000269|PubMed:16389068, ECO:0000269|PubMed:16669787, ECO:0000269|PubMed:17721439, ECO:0000269|PubMed:18270262, ECO:0000269|PubMed:19665974, ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:22052202, ECO:0000269|PubMed:23386621, ECO:0000269|PubMed:25362046, ECO:0000269|PubMed:28688764, ECO:0000269|PubMed:29581290, ECO:0000269|PubMed:34289367}.
uniprot
proteins
mass_dalton
58022
uniprot
proteins
mutagenesis
MUTAGEN 46; /note="K->A: Loss of autophosphorylation and kinase activity."; /evidence="ECO:0000269|PubMed:14707132, ECO:0000269|PubMed:16389068"; MUTAGEN 46; /note="K->M: Loss of RELT, RELL1 and RELL2 phosphorylation. Retention of some autophosphorylation activity may be due to complex formation with other endogenous kinases in the assay."; /evidence="ECO:0000269|PubMed:14707132, ECO:0000269|PubMed:16389068"; MUTAGEN 185; /note="T->A: Prevents phosphorylation and activation by WNK kinases."; /evidence="ECO:0000269|PubMed:17190791"; MUTAGEN 185; /note="T->E,D: Mimics phosphorylation; promoting kinase activity independently of WNK kinases."; /evidence="ECO:0000269|PubMed:16669787, ECO:0000269|PubMed:16832045, ECO:0000269|PubMed:17190791, ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:23386621, ECO:0000269|PubMed:29581290"; MUTAGEN 325; /note="S->A: Does not affect activation by WNK kinases."; /evidence="ECO:0000269|PubMed:17190791"; MUTAGEN 325; /note="S->E,D: Mimics phosphorylation; slightly promoting kinase activity independently of WNK kinases."; /evidence="ECO:0000269|PubMed:17190791, ECO:0000269|PubMed:23386621, ECO:0000269|PubMed:29581290"; MUTAGEN 446; /note="E->A: Decreased interaction with WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 448; /note="N->A: Decreased interaction with WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 449; /note="D->A: Decreased interaction with WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 450; /note="I->A: Abolished interaction with WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 451; /note="R->A: Decreased interaction with WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 459; /note="D->A: Abolished interaction with SLC12A2/NKCC1 and WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 467; /note="E->A: Abolished interaction with WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 468; /note="L->F: Abolished interaction with WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 471; /note="A->L: Decreased interaction with WNK4."; /evidence="ECO:0000269|PubMed:17721439"; MUTAGEN 473; /note="L->A: Abolished interaction with SLC12A2/NKCC1 and WNK4."; /evidence="ECO:0000269|PubMed:17721439
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Thr-185 by WNK kinases (WNK1, WNK2, WNK3 or WNK4) is required for activation (PubMed:16669787, PubMed:16832045, PubMed:17190791, PubMed:21321328, PubMed:22989884, PubMed:29581290). Autophosphorylated; promoting its activity (PubMed:14707132, PubMed:22052202). {ECO:0000269|PubMed:14707132, ECO:0000269|PubMed:16669787, ECO:0000269|PubMed:16832045, ECO:0000269|PubMed:17190791, ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:22052202, ECO:0000269|PubMed:22989884, ECO:0000269|PubMed:29581290}.
uniprot
proteins
protein_family
Protein kinase superfamily
uniprot
proteins
protein_family
STE Ser/Thr protein kinase family
uniprot
proteins
protein_family
STE20 subfamily
uniprot
proteins
pubmed
10083736
uniprot
proteins
pubmed
10470851
uniprot
proteins
pubmed
14707132
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16389068
uniprot
proteins
pubmed
16669787
uniprot
proteins
pubmed
16832045
uniprot
proteins
pubmed
17081983
uniprot
proteins
pubmed
17190791
uniprot
proteins
pubmed
17344846
uniprot
proteins
pubmed
17525332
uniprot
proteins
pubmed
17721439
uniprot
proteins
pubmed
18270262
uniprot
proteins
pubmed
18318008
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19665974
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21321328
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
22052202
uniprot
proteins
pubmed
22223895
uniprot
proteins
pubmed
22361696
uniprot
proteins
pubmed
22989884
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
23386621
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
25362046
uniprot
proteins
pubmed
28688764
uniprot
proteins
pubmed
29581290
uniprot
proteins
pubmed
34289367
uniprot
proteins
sequence_length
527
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22361696, ECO:0000269|PubMed:22989884}.
uniprot
proteins