Gm12250
entrez
631323
canonical
uniprot
Q0GUM3
canonical
genesymbol
Gm12250
resolver
genesymbol_syn
Irgb10
resolver
uniprot
Q0GUM2
resolver
uniprot_entry
IRB10_MOUSE
resolver
uniprot_sec
Q0GUM2
resolver
amino_acid_sequence
MGQSSSKPDAKAHNMASSLTEFFKNFKMESKIISKETIDSIQSCIQEGDIQKVISIINAALTDIEKAPLNIAVTGETGAGKSTFINALRGIGHEESESAESGAVETTKDRKKYTHPKFPNVTIWDLPGVGTTNFKPEEYLKKMKFQEYDFFLIISSARFRDNEAQLAEAIKKMKKKFYFVRTKIDSDLWNEKKAKPSSYNREKILEVIRSDCVKNLQNANAASTRVFLVSSFEVAQFDFPSLESTLLEELPAHKRHIFVQCLPTITEPAIDRRRDVLKQTIWLEALKAGASATIPMMSFFNDDIEEFEKILSHYRACFGLDDESLENMAKEWSMSVEELESTIKSPHLLSSEPNESVADKLVKTMEKIFAVTGGFVATGLYFRKSYYMQNYFLDTVTEDAKVLLKKKVFLQDSVDSE
uniprot
proteins
ec_number
3.6.5.-
uniprot
proteins
function
FUNCTION: Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens by mediating cytosolic release of pathogenic ligands that activate the inflammasomes (PubMed:16959883, PubMed:20109161, PubMed:27693356, PubMed:30062052, PubMed:30510167, PubMed:34694641). Following infection, recruited to the membrane of pathogens in a GBP-dependent manner and mediates disruption of the pathogen membrane, liberating ligands that are detected by inflammasomes, such as lipopolysaccharide (LPS) that activates the non-canonical CASP4/CASP11 inflammasome or double-stranded DNA (dsDNA) that activates the AIM2 inflammasome (PubMed:27693356, PubMed:30062052, PubMed:30510167). Promotes AIM2 and NLRP3 inflammasome activation following A.fumigatus infection by liberating beta-glucan, which directly triggers inflammasome assembly (PubMed:30510167). Promotes NLRP3 inflammasome activation following influenza A virus infection (PubMed:34694641). {ECO:0000269|PubMed:16959883, ECO:0000269|PubMed:20109161, ECO:0000269|PubMed:27693356, ECO:0000269|PubMed:30062052, ECO:0000269|PubMed:30510167, ECO:0000269|PubMed:34694641}.
uniprot
proteins
mass_dalton
47264
uniprot
proteins
mutagenesis
MUTAGEN 81; /note="K->A: Abolished GTPase activity, promoting localization to lipid droplets."; /evidence="ECO:0000269|PubMed:23785284"; MUTAGEN 82; /note="S->N: Abolished localization to pathogen-containing vacuoles."; /evidence="ECO:0000269|PubMed:23785284"; MUTAGEN 132; /note="T->W: Does not affect formation of a head-to-head homodimer in presence of GDP."; /evidence="ECO:0000269|PubMed:33469160"; MUTAGEN 185; /note="D->R: Impaired formation of a head-to-head homodimer in presence of GDP."; /evidence="ECO:0000269|PubMed:33469160"; MUTAGEN 193; /note="K->E: Impaired formation of a head-to-head homodimer in presence of GDP."; /evidence="ECO:0000269|PubMed:33469160"; MUTAGEN 206; /note="E->K: Does not affect formation of a head-to-head homodimer in presence of GDP."; /evidence="ECO:0000269|PubMed:33469160
uniprot
proteins
post_translational_modification
PTM: Myristoylation is required for localization to pathogen-containing vacuoles (PubMed:23785284). {ECO:0000305|PubMed:23785284}.; PTM: (Microbial infection) Phosphorylated by Toxoplasma gondii ROP18. {ECO:0000269|PubMed:21147463}.
uniprot
proteins
protein_family
GB1 subfamily
uniprot
proteins
protein_family
GB1/RHD3 GTPase family
uniprot
proteins
protein_family
TRAFAC class dynamin-like GTPase superfamily
uniprot
proteins
pubmed
16959883
uniprot
proteins
pubmed
18424746
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
20109161
uniprot
proteins
pubmed
21147463
uniprot
proteins
pubmed
23785284
uniprot
proteins
pubmed
27693356
uniprot
proteins
pubmed
30062052
uniprot
proteins
pubmed
30510167
uniprot
proteins
pubmed
33469160
uniprot
proteins
pubmed
34694641
uniprot
proteins
sequence_length
417
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:27693356}; Multi-pass membrane protein {ECO:0000269|PubMed:27693356}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:23785284}; Multi-pass membrane protein {ECO:0000269|PubMed:27693356}. Note=Recruited to the membrane of pathogens in the cytosol in a GBP (Gbp1, Gbp2, Gbp3, Gbp5 ad/or Gbp7)-dependent manner (PubMed:27693356). Recruited to the membrane of pathogen-containing vacuoles: vacuoles may serve as a cellular transport mechanism to deliver Gm12250/Irgb10 to the pathogen membrane (Probable) (PubMed:23785284, PubMed:27693356). {ECO:0000269|PubMed:27693356, ECO:0000305|PubMed:23785284, ECO:0000305|PubMed:27693356}.
uniprot
proteins
transmembrane_region
TRANSMEM 284..302; /note="Helical"; /evidence="ECO:0000305|PubMed:27693356"; TRANSMEM 370..387; /note="Helical"; /evidence="ECO:0000305|PubMed:27693356
uniprot
proteins