Pofut1
entrez
311551
canonical
uniprot
Q6EV70
canonical
ensembl
ENSRNOG00000010104
resolver
ensembl
ENSRNOG00055024966
resolver
ensembl
ENSRNOG00060020299
resolver
ensembl
ENSRNOG00065024520
resolver
ensp
ENSRNOP00000013730
resolver
ensp
ENSRNOP00055035117
resolver
ensp
ENSRNOP00060028940
resolver
ensp
ENSRNOP00065034392
resolver
enst
ENSRNOT00055043029
resolver
enst
ENSRNOT00060035198
resolver
enst
ENSRNOT00065042065
resolver
genesymbol
Pofut1
resolver
genesymbol_syn
Fut12
resolver
uniprot_entry
OFUT1_RAT
resolver
activity_regulation
ACTIVITY REGULATION: Activated by manganese and, to a lesser extent, by calcium. {ECO:0000269|PubMed:9023546}.
uniprot
proteins
amino_acid_sequence
MGAAAWAPSHLLLRASFLLLLLLLPLPLRGRAGGSWDLAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPFHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYKEQWIQRFPPKEHPVLALPGAPAQFPVLEEHRELQKYMVWSDEMVRTGEAQISTHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTATPLTTTMCLPDLKEIQRAVKLWVRALDARSVYIATDSESYVSEIQQLFKEKVKVVSLKPEVAQIDLYILGQADHFIGNCVSSFTAFVKRERDLHGRPSSFFGMDRPSQLRDEF
uniprot
proteins
ec_number
2.4.1.221
uniprot
proteins
function
FUNCTION: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs) (By similarity). Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. {ECO:0000250, ECO:0000269|PubMed:15653671, ECO:0000269|PubMed:9023546}.
uniprot
proteins
mass_dalton
44983
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein glycosylation. {ECO:0000269|PubMed:15653671, ECO:0000269|PubMed:9023546}.
uniprot
proteins
post_translational_modification
PTM: N-glycosylated. {ECO:0000250|UniProtKB:P83337}.
uniprot
proteins
protein_family
Glycosyltransferase 65 family
uniprot
proteins
pubmed
12966037
uniprot
proteins
pubmed
15653671
uniprot
proteins
pubmed
9023546
uniprot
proteins
sequence_length
395
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000269|PubMed:15653671}.
uniprot
proteins