P36370
uniprot
P36370
canonical
amino_acid_sequence
MAAHAWPTAALLLLLVDWLLLRPVLPGIFSLLVPEVPLLRVWAVGLSRWAILGLGVRGVLGVTAGARGWLAALQPLVAALGLALPGLASFRKLSAWGALREGDNAGLLHWNSRLDAFVLSYVAALPAAALWHKLGGFWAPSGHKGAGDMLCRMLGFLDSKKGRLHLVLVLLILSCLGEMAIPFFTGRITDWILQDKTAPSFARNMWLMCILTIASTVLEFAGDGIYNITMGHMHSRVHGEVFRAVLHQETGFFLKNPTGSITSRVTEDTSNVCESISDKLNLFLWYLGRGLCLLAFMIWGSFYLTVVTLLSLPLLFLLPRRLGKVYQSLAVKVQESLAKSTQVALEALSAMPTVRSFANEEGEAQKFRQKLEEMKPLNKKEALAYVTEVWTMSVSGMLLKVGILYLGGQLVVRGAVSSGNLVSFVLYQLQFTRAVEVLLSIYPSMQKSVGASEKIFEYLDRTPCSPLSGSLAPLNMKGLVKFQDVSFAYPNHPNVQVLQGLTFTLYPGKVTALVGPNGSGKSTVAALLQNLYQPTGGKVLLDGEPLVQYDHHYLHTQVAAVGQEPLLFGRSFRENIAYGLTRTPTMEEITAVAMESGAHDFISGFPQGYDTEVGETGNQLSGGQRQAVALARALIRKPRLLILDDATSALDAGNQLRVQRLLYESPEWASRTVLLITQQLSLAERAHHILFLKEGSVCEQGTHLQLMERGGCYRSMVEALAAPSD
uniprot
proteins
ec_number
7.4.2.14
uniprot
proteins
function
FUNCTION: ABC transporter associated with antigen processing (PubMed:17018292). In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules (By similarity). Uses the chemical energy of ATP to export peptides against the concentration gradient (By similarity). During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle (By similarity). As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation (By similarity). {ECO:0000250|UniProtKB:Q03518, ECO:0000269|PubMed:17018292}.
uniprot
proteins
mass_dalton
79150
uniprot
proteins
mutagenesis
MUTAGEN 621..622; /note="SG->AV: Complete loss of ATPase activity; when associated with N-645. Impairs peptide loading onto MHCI."; /evidence="ECO:0000269|PubMed:17018292"; MUTAGEN 645; /note="D->E: Increases ATPase activity by 8-fold; when associated with H-678."; /evidence="ECO:0000269|PubMed:17018292"; MUTAGEN 645; /note="D->N: Complete loss of ATPase activity; when associated with A-621 and V-622."; /evidence="ECO:0000269|PubMed:17018292"; MUTAGEN 645; /note="D->Q: Impairs ATPase activity; when associated with H-678."; /evidence="ECO:0000269|PubMed:17018292"; MUTAGEN 651; /note="D->A,N: Decreases ATP-driven nucleotide binding domain (NBD) dimerization."; /evidence="ECO:0000269|PubMed:25377891"; MUTAGEN 678; /note="Q->H: Increases ATPase activity by 8-fold; when associated with E-645. Impairs ATPase activity; when associated with Q-645."; /evidence="ECO:0000269|PubMed:17018292
uniprot
proteins
protein_family
ABC transporter superfamily
uniprot
proteins
protein_family
ABCB family
uniprot
proteins
protein_family
MHC peptide exporter (TC 3.A.1.209) subfamily
uniprot
proteins
pubmed
17018292
uniprot
proteins
pubmed
1979660
uniprot
proteins
pubmed
25377891
uniprot
proteins
sequence_length
725
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q03518}; Multi-pass membrane protein {ECO:0000255}. Note=The transmembrane segments seem to form a pore in the membrane.
uniprot
proteins
transmembrane_region
TRANSMEM 9..29; /note="Helical; Name=1"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 39..60; /note="Helical; Name=2"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 68..88; /note="Helical; Name=3"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 111..131; /note="Helical; Name=4"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 164..184; /note="Helical; Name=5"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 205..225; /note="Helical; Name=6"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 276..296; /note="Helical; Name=7"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 306..326; /note="Helical; Name=8"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 396..416; /note="Helical; Name=9"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"; TRANSMEM 421..441; /note="Helical; Name=10"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00441
uniprot
proteins