AKAP8
entrez
10270
canonical
uniprot
O43823
canonical
ensembl
ENSG00000105127
resolver
ensp
ENSP00000269701
resolver
enst
ENST00000269701
resolver
genesymbol
AKAP8
resolver
genesymbol_syn
AKAP95
resolver
hgnc
HGNC:378
resolver
uniprot_entry
AKAP8_HUMAN
resolver
amino_acid_sequence
MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTSVTTGATYSYGPASWEAAKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE
uniprot
proteins
function
FUNCTION: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). May play a repressive role in the regulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro. In cells, associates with ribosomal RNA (rRNA) chromatin, preferentially with rRNA promoter and transcribed regions (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity). {ECO:0000250|UniProtKB:Q63014, ECO:0000250|UniProtKB:Q9DBR0, ECO:0000269|PubMed:10601332, ECO:0000269|PubMed:10791967, ECO:0000269|PubMed:11964380, ECO:0000269|PubMed:16980585, ECO:0000269|PubMed:22130794, ECO:0000269|PubMed:26683827, ECO:0000269|PubMed:26880274, ECO:0000305|PubMed:14641107, ECO:0000305|PubMed:9473338}.
uniprot
proteins
mass_dalton
76108
uniprot
proteins
mutagenesis
MUTAGEN 290; /note="R->S: No nuclear localization; when associated with 304-N-S-305."; /evidence="ECO:0000269|PubMed:16227597"; MUTAGEN 304..305; /note="KR->NS: No nuclear localization; when associated with S-290."; /evidence="ECO:0000269|PubMed:16227597"; MUTAGEN 392; /note="C->S: Abolishes chromosome-condensation activity; when associated with S-395."; /evidence="ECO:0000269|PubMed:11964380"; MUTAGEN 395; /note="C->S: Abolishes chromosome-condensation activity; when associated with S-392."; /evidence="ECO:0000269|PubMed:11964380"; MUTAGEN 481; /note="C->S: Abolishes chromosome-condensation activity and recruitment of condensin complex; when associated with S-484."; /evidence="ECO:0000269|PubMed:11964380"; MUTAGEN 484; /note="C->S: Abolishes chromosome-condensation activity and recruitment of condensin complex; when associated with S-481."; /evidence="ECO:0000269|PubMed:11964380"; MUTAGEN 582; /note="I->P: No effect on activity to regulate DNA replication and on condensin complex recruitment."; /evidence="ECO:0000269|PubMed:11964380, ECO:0000269|PubMed:12740381
uniprot
proteins
post_translational_modification
PTM: Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes. {ECO:0000269|PubMed:25770215}.
uniprot
proteins
protein_family
AKAP95 family
uniprot
proteins
pubmed
10601332
uniprot
proteins
pubmed
10764601
uniprot
proteins
pubmed
10791967
uniprot
proteins
pubmed
11591814
uniprot
proteins
pubmed
11964380
uniprot
proteins
pubmed
12740381
uniprot
proteins
pubmed
14641107
uniprot
proteins
pubmed
15057824
uniprot
proteins
pubmed
15470020
uniprot
proteins
pubmed
16227597
uniprot
proteins
pubmed
16959974
uniprot
proteins
pubmed
16980585
uniprot
proteins
pubmed
17081983
uniprot
proteins
pubmed
18669648
uniprot
proteins
pubmed
18691976
uniprot
proteins
pubmed
19277197
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
22130794
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
23995757
uniprot
proteins
pubmed
24129315
uniprot
proteins
pubmed
24275569
uniprot
proteins
pubmed
25770215
uniprot
proteins
pubmed
26683827
uniprot
proteins
pubmed
26880274
uniprot
proteins
pubmed
28112733
uniprot
proteins
pubmed
9473338
uniprot
proteins
sequence_length
692
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19277197, ECO:0000269|PubMed:26683827}. Nucleus matrix {ECO:0000269|PubMed:10601332}. Nucleus, nucleolus {ECO:0000269|PubMed:26683827}. Cytoplasm {ECO:0000250|UniProtKB:Q9DBR0}. Note=Associated with the nuclear matrix in interphase and redistributes mostly to chromatin at mitosis. However, mitotic chromatin localization has been questioned. Upon nuclear reassembly at the end of mitosis, is sequestered into the daughter nuclei where it re-acquires an interphase distribution. Exhibits partial localization to the nucleolus in interphase, where it colocalizes with UBTF/UBF, suggesting localization to the fibrillary center and/or to the dense fibrillary component. Colocalizes with GJA1 at the nuclear membrane specifically during cell cycle G1/S phase. {ECO:0000269|PubMed:10601332, ECO:0000269|PubMed:26683827, ECO:0000269|PubMed:26880274}.
uniprot
proteins