Knl1
entrez
76464
canonical
uniprot
Q66JQ7
canonical
ensembl
ENSMUSG00000027326
resolver
ensp
ENSMUSP00000028799
resolver
ensp
ENSMUSP00000028802
resolver
ensp
ENSMUSP00000097140
resolver
genesymbol
Knl1
resolver
genesymbol_syn
Casc5
resolver
uniprot
A3KGI3
resolver
uniprot
A3KGI4
resolver
uniprot
Q8CCH6
resolver
uniprot
Q9CV38
resolver
uniprot_entry
KNL1_MOUSE
resolver
uniprot_sec
A3KGI3
resolver
uniprot_sec
A3KGI4
resolver
uniprot_sec
Q8CCH6
resolver
uniprot_sec
Q9CV38
resolver
amino_acid_sequence
MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEFSITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLENLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAEIPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPSTEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATAPAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTSKSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPLREKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSKDEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVIPTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKIMYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSRVVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITKSCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDITPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVTSLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQRRKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSAYGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQAKEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSSMKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDKYSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFPPCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQPHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHFAVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSSTKPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDEESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAADLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESVLIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDINKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLKEKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQIYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKSRYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETKNLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKEQTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD
uniprot
proteins
function
FUNCTION: Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore. Can bind either to microtubules or to the protein phosphatase 1 (PP1) catalytic subunits PPP1CA and PPP1CC (via overlapping binding sites), it has higher affinity for PP1. Recruits MAD2L1 to the kinetochore and also directly links BUB1 and BUB1B to the kinetochore (By similarity). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (PubMed:35167144, PubMed:36904774). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (PubMed:35167144, PubMed:36904774). {ECO:0000250|UniProtKB:Q8NG31, ECO:0000269|PubMed:35167144, ECO:0000269|PubMed:36904774}.
uniprot
proteins
mass_dalton
238185
uniprot
proteins
post_translational_modification
PTM: Phosphorylation by AURKB negatively regulates its interaction with protein phosphatase 1 (PP1) subunit PPP1CA and with microtubules. {ECO:0000250|UniProtKB:Q8NG31}.
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21183079
uniprot
proteins
pubmed
35167144
uniprot
proteins
pubmed
36904774
uniprot
proteins
sequence_length
2119
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8NG31}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:35167144}. Cytoplasm {ECO:0000269|PubMed:35167144}. Note=Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle (By similarity). Localizes to the cytoplasm during meiotic prophase I and then the nucleus as meiosis progresses (PubMed:35167144). {ECO:0000250|UniProtKB:Q8NG31, ECO:0000269|PubMed:35167144}.
uniprot
proteins