Ap2m1
entrez
116563
canonical
uniprot
P84092
canonical
ensembl
ENSRNOG00000001709
resolver
ensembl
ENSRNOG00055004882
resolver
ensembl
ENSRNOG00060011929
resolver
ensembl
ENSRNOG00065004219
resolver
ensp
ENSRNOP00000054900
resolver
ensp
ENSRNOP00000079141
resolver
ensp
ENSRNOP00055005873
resolver
ensp
ENSRNOP00060015872
resolver
ensp
ENSRNOP00065004397
resolver
enst
ENSRNOT00000058092
resolver
enst
ENSRNOT00055007791
resolver
enst
ENSRNOT00060020201
resolver
enst
ENSRNOT00065006162
resolver
genesymbol
Ap2m1
resolver
uniprot
A0A140TAH5
resolver
uniprot
P20172
resolver
uniprot
P53679
resolver
uniprot_entry
A0A140TAH5_RAT
resolver
uniprot_entry
AP2M1_RAT
resolver
uniprot_sec
P20172
resolver
uniprot_sec
P53679
resolver
amino_acid_sequence
MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC
uniprot
proteins
function
FUNCTION: Component of the adaptor protein complex 2 (AP-2) (PubMed:14745134, PubMed:15473838). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways (PubMed:14745134, PubMed:15473838). Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation (PubMed:14745134, PubMed:15473838). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome (PubMed:14745134, PubMed:15473838). The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components (PubMed:14745134, PubMed:15473838). Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation (PubMed:14745134, PubMed:15473838). AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis (PubMed:14745134, PubMed:15473838). AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface (By similarity). AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules (PubMed:15985462). AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway (By similarity). During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (By similarity). The AP-2 mu (AP2M1) subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs (PubMed:15985462). The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2 (PubMed:11516654, PubMed:15985462). The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (PubMed:20947020). {ECO:0000250|UniProtKB:Q96CW1, ECO:0000269|PubMed:11516654, ECO:0000269|PubMed:14745134, ECO:0000269|PubMed:15473838, ECO:0000269|PubMed:15985462, ECO:0000269|PubMed:20947020}.
uniprot
proteins
mass_dalton
49655
uniprot
proteins
mutagenesis
MUTAGEN 156; /note="T->A: Inhibits endocytosis by AP-2; no effect on membrane association of AP-2."; /evidence="ECO:0000269|PubMed:11516654"; MUTAGEN 176; /note="D->A: Abolishes interaction with TTGN1 and EGFR."; /evidence="ECO:0000269|PubMed:10228163"; MUTAGEN 421; /note="W->A: Abolishes interaction with TTGN1 and EGFR."; /evidence="ECO:0000269|PubMed:10228163
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Thr-156 increases the affinity of the AP-2 complex for cargo membrane proteins during the initial stages of endocytosis. {ECO:0000250|UniProtKB:Q96CW1}.
uniprot
proteins
protein_family
Adaptor complexes medium subunit family
uniprot
proteins
pubmed
10228163
uniprot
proteins
pubmed
11247301
uniprot
proteins
pubmed
11516654
uniprot
proteins
pubmed
12086608
uniprot
proteins
pubmed
12121421
uniprot
proteins
pubmed
14745134
uniprot
proteins
pubmed
15473838
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15985462
uniprot
proteins
pubmed
19140243
uniprot
proteins
pubmed
20947020
uniprot
proteins
pubmed
25061211
uniprot
proteins
pubmed
3148444
uniprot
proteins
pubmed
9812899
uniprot
proteins
sequence_length
435
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q96CW1}. Membrane, coated pit {ECO:0000250|UniProtKB:Q96CW1}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96CW1}; Cytoplasmic side {ECO:0000250|UniProtKB:Q96CW1}. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. {ECO:0000250|UniProtKB:P84091}.
uniprot
proteins