ENDOG
entrez
2021
canonical
uniprot
Q14249
canonical
ensembl
ENSG00000167136
resolver
ensp
ENSP00000361725
resolver
ensp
ENSP00000788934
resolver
enst
ENST00000372642
resolver
genesymbol
ENDOG
resolver
hgnc
HGNC:3346
resolver
uniprot
E5KNL5
resolver
uniprot
Q5T281
resolver
uniprot
Q9BSP2
resolver
uniprot_entry
E5KNL5_HUMAN
resolver
uniprot_entry
NUCG_HUMAN
resolver
uniprot_sec
Q5T281
resolver
uniprot_sec
Q9BSP2
resolver
amino_acid_sequence
MRALRAGLTLASGAGLGAVVEGWRRRREDARAAPGLLGRLPVLPVAAAAELPPVPGGPRGPGELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK
uniprot
proteins
ec_number
3.1.30.-
uniprot
proteins
function
FUNCTION: Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (PubMed:25355512). The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (By similarity). Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs (By similarity). Promotes conservative recombination when the DNA is 5hmC-modified (PubMed:25355512). Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response (PubMed:33473107). GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation (PubMed:33473107). Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication (PubMed:29719607). {ECO:0000250|UniProtKB:O08600, ECO:0000269|PubMed:25355512, ECO:0000269|PubMed:29719607, ECO:0000269|PubMed:33473107}.
uniprot
proteins
mass_dalton
32620
uniprot
proteins
mutagenesis
MUTAGEN 128; /note="T->A: Loss of phosphorylation. Suppresses interaction with YWHAG and induction of autophagy; when associated with A-288."; /evidence="ECO:0000269|PubMed:33473107"; MUTAGEN 128; /note="T->D: Phosphomimetic mutant. No effect on its interaction with YWHAG. Enhances interaction with YWHAG; when associated with T-288."; /evidence="ECO:0000269|PubMed:33473107"; MUTAGEN 288; /note="S->A: Loss of phosphorylation. Suppresses interaction with YWHAG and induction of autophagy; when associated with A-128."; /evidence="ECO:0000269|PubMed:33473107"; MUTAGEN 288; /note="S->D: Phosphomimetic mutant. No effect on its interaction with YWHAG. Enhances interaction with YWHAG; when associated with T-128."; /evidence="ECO:0000269|PubMed:33473107
uniprot
proteins
post_translational_modification
PTM: GSK3-beta-mediated dual phosphorylations at Thr-128 and Ser-288 is necessary for its interaction with YWHAG and the induction of autophagy. {ECO:0000269|PubMed:33473107}.
uniprot
proteins
protein_family
DNA/RNA non-specific endonuclease family
uniprot
proteins
pubmed
15164053
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
25355512
uniprot
proteins
pubmed
29719607
uniprot
proteins
pubmed
33473107
uniprot
proteins
pubmed
7789991
uniprot
proteins
sequence_length
297
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:33473107}.
uniprot
proteins