Rnf146
entrez
68031
canonical
uniprot
Q9CZW6
canonical
ensembl
ENSMUSG00000038876
resolver
ensp
ENSMUSP00000037224
resolver
ensp
ENSMUSP00000124215
resolver
ensp
ENSMUSP00000124288
resolver
ensp
ENSMUSP00000124772
resolver
ensp
ENSMUSP00000273714
resolver
enst
ENSMUST00000037548
resolver
enst
ENSMUST00000160144
resolver
enst
ENSMUST00000160372
resolver
enst
ENSMUST00000162335
resolver
genesymbol
Rnf146
resolver
genesymbol_syn
Echdc1
resolver
uniprot
E0CX97
resolver
uniprot
Q3TF93
resolver
uniprot
Q3U6Y7
resolver
uniprot
Q9CTM5
resolver
uniprot_entry
Q9CTM5_MOUSE
resolver
uniprot_entry
RN146_MOUSE
resolver
uniprot_sec
E0CX97
resolver
uniprot_sec
Q3TF93
resolver
uniprot_sec
Q3U6Y7
resolver
amino_acid_sequence
MEMAGCGEIDHSINMLPTNKKANESCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDTNTVNLARESSADGADSGSAQTGASVQLAVPSSTRPLTSVDGQLTSPVTPSPDAGISLEDSFAHLQLSGDSIAERSHRGEGEEDHESPSSGRVPDTSVEETESDASSDSEDAPVVVAQHSLTQQRPLVPNGNQTVADQSDRSGTDRSVAGGGTMSVNVRSRRPDGQCTVTEV
uniprot
proteins
ec_number
2.3.2.27
uniprot
proteins
function
FUNCTION: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein (By similarity). Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos (PubMed:21602803, PubMed:21825151). Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner (PubMed:21602803, PubMed:21825151). Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest (PubMed:21602803, PubMed:21825151). Promotes cell survival after gamma-irradiation. Facilitates DNA repair (By similarity). {ECO:0000250|UniProtKB:Q9NTX7, ECO:0000269|PubMed:21602803, ECO:0000269|PubMed:21825151}.
uniprot
proteins
mass_dalton
38934
uniprot
proteins
mutagenesis
MUTAGEN 55; /note="H->A: Loss of ubiquitination activity."; /evidence="ECO:0000269|PubMed:21825151"; MUTAGEN 61; /note="C->A: Loss of ubiquitination activity. Loss of protection against DNA damage. No effect on PAR-binding."; /evidence="ECO:0000269|PubMed:21825151"; MUTAGEN 156; /note="Y->A: Loss of PAR-binding and of protection against DNA damage; when associated with A-157."; /evidence="ECO:0000269|PubMed:21602803, ECO:0000269|PubMed:21825151"; MUTAGEN 157; /note="R->A: Loss of PAR-binding and of protection against DNA damage; when associated with A-156."; /evidence="ECO:0000269|PubMed:21602803, ECO:0000269|PubMed:21825151
uniprot
proteins
pathway_participation
PATHWAY: Protein modification; protein ubiquitination.
uniprot
proteins
post_translational_modification
PTM: Ubiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon PAR-binding (By similarity). {ECO:0000250}.
uniprot
proteins
pubmed
15489334
uniprot
proteins
pubmed
15813938
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
21602803
uniprot
proteins
pubmed
21825151
uniprot
proteins
sequence_length
359
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:21602803}. Nucleus {ECO:0000250}. Note=Translocates to the nucleus after DNA damage, such as laser-induced DNA breaks, and concentrates at DNA breaks. This translocation requires PARP1 activation and PAR-binding (By similarity). {ECO:0000250}.
uniprot
proteins