Ficd
entrez
288741
canonical
uniprot
Q6AY47
canonical
ensembl
ENSRNOG00000059799
resolver
ensembl
ENSRNOG00055002212
resolver
ensembl
ENSRNOG00060006941
resolver
ensembl
ENSRNOG00065007053
resolver
ensp
ENSRNOP00000073778
resolver
ensp
ENSRNOP00055002616
resolver
ensp
ENSRNOP00060008478
resolver
ensp
ENSRNOP00065008080
resolver
enst
ENSRNOT00055003510
resolver
enst
ENSRNOT00060011354
resolver
enst
ENSRNOT00065011001
resolver
genesymbol
Ficd
resolver
uniprot_entry
FICD_RAT
resolver
activity_regulation
ACTIVITY REGULATION: The side chain of Glu-234 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. In response to endoplasmic reticulum stress, mediates de-AMPylase activity (By similarity). Adenylyltransferase activity is inhibited by the inhibitory helix present at the N-terminus: Glu-234 binds ATP and competes with ATP-binding at Arg-374, thereby preventing adenylyltransferase activity (By similarity). In unstressed cells, disengagement of Glu-234 promotes adenylyltransferase activity (By similarity). Activation dissociates ATP-binding from Glu-234, allowing ordered binding of the entire ATP moiety with the alpha-phosphate in an orientation that is productive for accepting an incoming target hydroxyl side chain (By similarity). {ECO:0000250|UniProtKB:A0A061I403, ECO:0000250|UniProtKB:Q9BVA6}.
uniprot
proteins
amino_acid_sequence
MILMPMASVVAVAEPKWVSVWGRFLWMTLLSMALGSLLALLLPLGAVEEQCLAVLRGFHLLRSKLDRAQHVVTKCTSPSTELSVTSRDAGLLTVKTKASPAGKLEAKAALNQALEMKRQGKRGKAHKLFLHALKMDPGFVDALNELGIFSEEDKDIIQADYLYTRALTISPFHEKALINRDRTLPLVEEIDQRYFSVLDSKVRKVMSIPKGSSALRRVMEETYYHHIYHTVAIEGNTLTLAEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINSTLVSRIGSVTIDHMLEIHRRVLGYVDPVEAGRFRRTQVLVGHHIPPHPRDVEKQMQEFTQWLNSEDAMNLHPVEFAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSEYYHVLEVANEGDVRPFIRFIAKCTEVTLDTLLLATTEYSAALPEAQPNHSGFKETLPVRP
uniprot
proteins
ec_number
2.7.7.108
uniprot
proteins
ec_number
3.1.4.-
uniprot
proteins
function
FUNCTION: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (By similarity). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (By similarity). {ECO:0000250|UniProtKB:A0A061I403, ECO:0000250|UniProtKB:Q9BVA6}.
uniprot
proteins
mass_dalton
51754
uniprot
proteins
post_translational_modification
PTM: Auto-AMPylated in vitro. {ECO:0000250|UniProtKB:Q9BVA6}.
uniprot
proteins
protein_family
Fic family
uniprot
proteins
pubmed
15489334
uniprot
proteins
sequence_length
458
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9BVA6}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q9BVA6}.
uniprot
proteins
transmembrane_region
TRANSMEM 24..44; /note="Helical; Signal-anchor for type II membrane protein"; /evidence="ECO:0000255
uniprot
proteins