STK39
entrez
27347
canonical
uniprot
Q9UEW8
canonical
ensembl
ENSG00000198648
resolver
ensp
ENSP00000348278
resolver
ensp
ENSP00000513185
resolver
ensp
ENSP00000724683
resolver
ensp
ENSP00000724684
resolver
ensp
ENSP00000724685
resolver
enst
ENST00000697205
resolver
genesymbol
STK39
resolver
genesymbol_syn
PASK
resolver
genesymbol_syn
SPAK
resolver
hgnc
HGNC:17717
resolver
uniprot
A0A8V8TKT5
resolver
uniprot
O14774
resolver
uniprot
Q53S90
resolver
uniprot
Q53SL7
resolver
uniprot
Q53SS1
resolver
uniprot
Q9UER4
resolver
uniprot
X5D9C8
resolver
uniprot_entry
A0A8V8TKT5_HUMAN
resolver
uniprot_entry
STK39_HUMAN
resolver
uniprot_sec
O14774
resolver
uniprot_sec
Q53S90
resolver
uniprot_sec
Q53SL7
resolver
uniprot_sec
Q53SS1
resolver
uniprot_sec
Q9UER4
resolver
uniprot_sec
X5D9C8
resolver
activity_regulation
ACTIVITY REGULATION: Activated following phosphorylation at Thr-231 by WNK kinases (WNK1, WNK2, WNK3 or WNK4) (PubMed:16832045, PubMed:17190791, PubMed:21321328, PubMed:34289367). Specifically inhibited by YU239252 (YU252) (PubMed:34289367). {ECO:0000269|PubMed:16832045, ECO:0000269|PubMed:17190791, ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:34289367}.
uniprot
proteins
amino_acid_sequence
MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS
uniprot
proteins
ec_number
2.7.11.1
uniprot
proteins
function
FUNCTION: Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed:16669787, PubMed:18270262, PubMed:21321328, PubMed:34289367). Specifically recognizes and binds proteins with a RFXV motif (PubMed:16669787, PubMed:21321328). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed:21321328). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (PubMed:12740379, PubMed:16669787, PubMed:21321328). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (PubMed:16669787, PubMed:19665974, PubMed:21321328). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed:18270262). Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities (By similarity). Phosphorylates RELT (By similarity). {ECO:0000250|UniProtKB:Q9Z1W9, ECO:0000269|PubMed:12740379, ECO:0000269|PubMed:16669787, ECO:0000269|PubMed:18270262, ECO:0000269|PubMed:19665974, ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:34289367}.
uniprot
proteins
mass_dalton
59474
uniprot
proteins
mutagenesis
MUTAGEN 92; /note="K->R: Abolished serine/threonine-protein kinase activity."; /evidence="ECO:0000269|PubMed:12740379"; MUTAGEN 231; /note="T->A: Prevents phosphorylation and activation by WNK kinases."; /evidence="ECO:0000269|PubMed:17190791"; MUTAGEN 231; /note="T->E,D: Mimics phosphorylation; promoting kinase activity independently of WNK kinases."; /evidence="ECO:0000269|PubMed:16832045, ECO:0000269|PubMed:17190791, ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:34289367"; MUTAGEN 371; /note="S->A: Does not affect activation by WNK kinases."; /evidence="ECO:0000269|PubMed:17190791"; MUTAGEN 371; /note="S->E: Does not greatly affect the kinase activity."; /evidence="ECO:0000269|PubMed:17190791"; MUTAGEN 477; /note="D->A: Abolished binding to WNK1 and SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16669787"; MUTAGEN 478; /note="T->A: Decreased binding to SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16669787"; MUTAGEN 482; /note="V->A: Decreased binding to SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16669787"; MUTAGEN 485; /note="E->A: Decreased binding to SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16669787"; MUTAGEN 491; /note="L->A: Abolished binding to WNK1 and SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16669787"; MUTAGEN 492; /note="V->A: Decreased binding to SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16669787"; MUTAGEN 496; /note="D->A: Decreased binding to SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16669787"; MUTAGEN 500; /note="V->A: Decreased binding to SLC12A2/NKCC1."; /evidence="ECO:0000269|PubMed:16669787
uniprot
proteins
post_translational_modification
PTM: Phosphorylation at Thr-231 by WNK kinases (WNK1, WNK2, WNK3 or WNK4) is required for activation (PubMed:16832045, PubMed:17190791, PubMed:21321328, PubMed:34289367). Autophosphorylation at Thr-231 positively regulates its activity (By similarity). Phosphorylated at Ser-309 by PRKCQ (PubMed:14988727). {ECO:0000250|UniProtKB:Q9Z1W9, ECO:0000269|PubMed:14988727, ECO:0000269|PubMed:16832045, ECO:0000269|PubMed:17190791, ECO:0000269|PubMed:21321328, ECO:0000269|PubMed:34289367}.
uniprot
proteins
protein_family
Protein kinase superfamily
uniprot
proteins
protein_family
STE Ser/Thr protein kinase family
uniprot
proteins
protein_family
STE20 subfamily
uniprot
proteins
pubmed
10980603
uniprot
proteins
pubmed
12740379
uniprot
proteins
pubmed
14988727
uniprot
proteins
pubmed
15815621
uniprot
proteins
pubmed
16669787
uniprot
proteins
pubmed
16832045
uniprot
proteins
pubmed
17190791
uniprot
proteins
pubmed
17487921
uniprot
proteins
pubmed
18088087
uniprot
proteins
pubmed
18270262
uniprot
proteins
pubmed
19413330
uniprot
proteins
pubmed
19608861
uniprot
proteins
pubmed
19665974
uniprot
proteins
pubmed
19690332
uniprot
proteins
pubmed
20068231
uniprot
proteins
pubmed
20385770
uniprot
proteins
pubmed
21269460
uniprot
proteins
pubmed
21321328
uniprot
proteins
pubmed
21406692
uniprot
proteins
pubmed
23186163
uniprot
proteins
pubmed
24722188
uniprot
proteins
pubmed
34289367
uniprot
proteins
sequence_length
545
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}. Note=Nucleus when caspase-cleaved. {ECO:0000305}.
uniprot
proteins