J3QMY9
uniprot
J3QMY9
canonical
amino_acid_sequence
MERTALAVCEILRYLIIHWKCEAGTAKGTLLDGQLVISIEALRSKHLPDSLHCIITIASTRSVYGGLNFKKFLQEIQPALPRLSAKLALASEEGGRSQDASGIAPCQVTFEVDENSQSLMTDCLVIKHFLRKIIIVHHKLKFSFSVAVNGTLSAETFGAENEPTLRLDNGVTLVVGFQRYVSKPKLNWSEAHCSRIHPVLGHPAPLFIPDAKADTGLLGELTLTPAAALCPSPKGFSSQLCRISSVSIFLYGPLGLPLLSSDQDQPSTAVFRDTSYFIDWKKYNLFMVPNLDLNLDTQSVLPDVNYKAESPEGNQSQNMNAQGPALLLFLFVDFQSDVPVQQAKIWGLHTLLTAHLSAILSESRSTVQQSIQSAVDQVWQLYHHDAKTQQRLQASLSVAVNSIMSVLTGSTRSSFRKTCLQALEAADTQEFGVKLHRIFYDITQHQFLKHCSCDTEQHLTPEKNISAQNTKDQHKNIAQEFPEESIGQAENKRPKRGSPNHGREESRVLGSARDRSPPKSATRDRELTEVSLTARGSQTQAAHGRAQAAEAASPAGGLEDLWLQEVSNLSEWLNPGHRS
uniprot
proteins
function
FUNCTION: Non-catalytic component of a topoisomerase 6 complex specifically required for double-strand breaks (DSB) formation that initiate meiotic recombination (PubMed:26917764, PubMed:36396648, PubMed:36732965, PubMed:38966985, PubMed:39972129, PubMed:39972130). Together with SPO11, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917764, PubMed:39972129, PubMed:39972130). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:26917764, PubMed:39972129, PubMed:39972130). Within the complex, TOP6BL may sense specific DNA architectures, such as bendable and underwound DNA for cleavage by SPO11 (PubMed:38966985). {ECO:0000269|PubMed:26917764, ECO:0000269|PubMed:36396648, ECO:0000269|PubMed:36732965, ECO:0000269|PubMed:38966985, ECO:0000269|PubMed:39972129, ECO:0000269|PubMed:39972130}.
uniprot
proteins
mass_dalton
63797
uniprot
proteins
mutagenesis
MUTAGEN 162..579; /note="Missing: Knockin mice display spermatogenic failure characterized by non-obstructive azoospermia due to defects in programmed meiotic DNA double-strand break formation, leading to meiotic arrest prior to pachytene stage of prophase I."; /evidence="ECO:0000269|PubMed:36732965"; MUTAGEN 562; /note="W->A: Abolished interaction with REC114; knocking mice are subfertile due to defects in programmed meiotic DNA double-strand break formation."; /evidence="ECO:0000269|PubMed:36396648
uniprot
proteins
protein_family
TOP6B-like family
uniprot
proteins
pubmed
16141072
uniprot
proteins
pubmed
19468303
uniprot
proteins
pubmed
26917764
uniprot
proteins
pubmed
36396648
uniprot
proteins
pubmed
36732965
uniprot
proteins
pubmed
38966985
uniprot
proteins
pubmed
39972129
uniprot
proteins
pubmed
39972130
uniprot
proteins
sequence_length
579
uniprot
proteins
subcellular_location
SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:39972129}. Nucleus {ECO:0000269|PubMed:39972129}. Note=Localizes to meiotic chromosomes together with SPO11. {ECO:0000269|PubMed:39972129}.
uniprot
proteins